FastQCFastQC Report
Tue 7 Mar 2017
1SN_17A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_17A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2983401
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG107700.078.753268
CACCGGC105450.077.4067849
CGCTGCA700.075.6277161
CAGCTGC850.074.7379761
CTTCACC118400.073.81666
GTGTGGC45700.069.328797
CCTTCAC132500.069.255935
CGGGGCA12000.066.4957394
TGCAGCC403.9677634E-666.174251
CGTAGTG24500.065.713424
TTCACCG134200.065.2657247
GGCAGCC250.003268965563.527281
GCAGCTC301.380481E-462.5842064
AACGGGC7900.060.0095445
GCGTAGT15150.059.7958073
ACCTTCA51250.059.7144974
TGTGGCA53650.059.1429568
GGCGCGC4700.058.922376
CAGCGTA11450.058.256461
GCAGCCA657.4578566E-1057.7777862
Produced by FastQC (version 0.11.5)