FastQCFastQC Report
Tue 7 Mar 2017
1SN_18A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_18A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3248512
Sequences flagged as poor quality0
Sequence length94
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA178890.5506828972772765No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG95250.2932111686827692No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT77010.23706238425469875No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA67880.20895720871586745No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA66990.20621749280901533No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC56750.1746953682178179No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG56740.17466458489302178No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG54960.16918515307931756No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG51420.15828785610150126No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT50340.15496325702352337No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG49520.1524390243902439No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC45710.14071057764293313No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA45070.1387404448559833No Hit
CTGCGCCGAGCGACAGAGCAGGCACGGACGAAACAGGACGTCCGAGCTCA44470.13689344536821782No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG43100.13267612987115332No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC42210.1299364139643012No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT42210.1299364139643012No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA42170.12981328066511683No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA40690.12525734859529533No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA40600.12498029867213051No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAA36340.11186660230899563No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA34240.10540210410181645No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA33040.1017081051262855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTC257.2908733E-787.999793
GCTGCCA850.087.999793
GCTGCAT700.087.999793
CTGCAGC1650.087.999791
AGCTGCC2400.087.999792
GCAGCTC450.087.999793
GCAGCCC158.9720194E-487.999793
GCAGCAA1250.087.999793
GCTGCCC2100.087.999783
AGCTGCG1050.087.999782
GCAGCGG401.8189894E-1187.999783
TGCAGCG202.5504887E-587.999782
AGCAGCC800.087.999782
CAGCTGC7050.087.375671
AGCAGCA2850.086.4559252
CAGCAGC6400.085.249791
AGCAGCG1500.085.066462
TGCTGCC1050.083.809322
AGCTGCA1900.083.368222
CAGCCCT185950.082.652121
Produced by FastQC (version 0.11.5)