FastQCFastQC Report
Tue 7 Mar 2017
1SN_18A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_18A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3248512
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG120650.080.530178
CACCGGC120400.076.954899
CTTCACC137050.074.386886
CCTTCAC153750.070.001045
GTGTGGC54850.068.715467
CGTAGTG26200.066.285114
GGCGCGC5900.066.030056
TTCACCG153950.065.275857
CGGGGCA15950.062.0922494
ACCTTCA62500.061.6564754
TGTGGCA63600.060.811498
GGGCCCG7700.058.5188878
GGCCCGA7750.057.5357139
AGGCGCG6950.057.4049955
TGTGTGG61750.057.3125766
CGGGCCC7800.057.166897
GCCTTCA45150.056.5531354
GCGCGCA6950.056.0542877
ATGTGTG61100.054.8494875
GCGTAGT16400.054.6644633
Produced by FastQC (version 0.11.5)