##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1SN_18A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 3248512 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.852763665333544 12.0 12.0 12.0 2.0 32.0 2 14.428185581583199 12.0 12.0 12.0 12.0 22.0 3 13.64069887997951 12.0 12.0 12.0 12.0 22.0 4 13.726127223787383 12.0 12.0 12.0 12.0 22.0 5 22.39897497685094 22.0 22.0 22.0 22.0 27.0 6 26.810270979451516 27.0 22.0 32.0 22.0 32.0 7 34.852770437665 37.0 37.0 37.0 27.0 41.0 8 37.49043777581859 41.0 37.0 41.0 27.0 41.0 9 38.45199709897947 41.0 37.0 41.0 32.0 41.0 10-11 38.81551199441468 41.0 41.0 41.0 34.5 41.0 12-13 39.00052208518854 41.0 41.0 41.0 37.0 41.0 14-15 38.94502652291264 41.0 41.0 41.0 37.0 41.0 16-17 38.84280141184641 41.0 41.0 41.0 37.0 41.0 18-19 38.77676040599511 41.0 41.0 41.0 34.5 41.0 20-21 38.788780524744865 41.0 41.0 41.0 34.5 41.0 22-23 38.79018455218882 41.0 41.0 41.0 34.5 41.0 24-25 38.7791967522361 41.0 41.0 41.0 34.5 41.0 26-27 38.7567509370444 41.0 41.0 41.0 32.0 41.0 28-29 38.689683769061034 41.0 41.0 41.0 32.0 41.0 30-31 38.595882822658496 41.0 41.0 41.0 32.0 41.0 32-33 38.64391327475472 41.0 41.0 41.0 32.0 41.0 34-35 38.611363294948575 41.0 41.0 41.0 32.0 41.0 36-37 38.524859997438824 41.0 41.0 41.0 32.0 41.0 38-39 38.446913848555894 41.0 41.0 41.0 32.0 41.0 40-41 38.46106417338154 41.0 41.0 41.0 32.0 41.0 42-43 38.247617370660784 41.0 41.0 41.0 32.0 41.0 44-45 38.363053607313134 41.0 41.0 41.0 32.0 41.0 46-47 38.33889716276252 41.0 41.0 41.0 32.0 41.0 48-49 38.29344743070058 41.0 41.0 41.0 32.0 41.0 50-51 38.19628525306355 41.0 41.0 41.0 32.0 41.0 52-53 38.17901796268569 41.0 39.0 41.0 32.0 41.0 54-55 38.04099954071279 41.0 37.0 41.0 32.0 41.0 56-57 37.82214133732614 41.0 37.0 41.0 27.0 41.0 58-59 37.819353137682725 41.0 37.0 41.0 27.0 41.0 60-61 37.79179621315852 41.0 37.0 41.0 27.0 41.0 62-63 37.75757993198117 41.0 37.0 41.0 27.0 41.0 64-65 37.71667227949289 41.0 37.0 41.0 27.0 41.0 66-67 37.61835618892589 41.0 37.0 41.0 27.0 41.0 68-69 37.64248031098546 41.0 37.0 41.0 27.0 41.0 70-71 37.645972371350325 41.0 37.0 41.0 27.0 41.0 72-73 37.4908364198747 41.0 37.0 41.0 27.0 41.0 74-75 37.41490996493164 41.0 37.0 41.0 27.0 41.0 76-77 36.31332206868868 39.0 34.5 41.0 24.5 41.0 78-79 36.829541956440366 41.0 37.0 41.0 27.0 41.0 80-81 37.50766720270697 41.0 37.0 41.0 27.0 41.0 82-83 37.67440985288033 41.0 37.0 41.0 27.0 41.0 84-85 37.59957759121715 41.0 37.0 41.0 27.0 41.0 86-87 37.491700507801724 41.0 37.0 41.0 27.0 41.0 88-89 37.415468528360066 41.0 37.0 41.0 27.0 41.0 90-91 37.24271758885298 41.0 37.0 41.0 27.0 41.0 92-93 37.160414983844916 41.0 37.0 41.0 27.0 41.0 94-95 37.005274568787186 41.0 37.0 41.0 27.0 41.0 96-97 36.94285214276567 41.0 37.0 41.0 27.0 41.0 98-99 36.81812457519012 41.0 37.0 41.0 27.0 41.0 100 36.69457462370464 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.5401297398627918 1101 2 -0.44784886686019654 1101 3 -0.03515934218202155 1101 4 -0.021652152269764358 1101 5 -1.0143660248818875 1101 6 -0.905789110894144 1101 7 -0.21898797716709595 1101 8 -0.08251504334567983 1101 9 -0.10659823597030282 1101 10-11 -0.06961917122363559 1101 12-13 0.06321248605902241 1101 14-15 0.03366286269467622 1101 16-17 -0.06506026790003716 1101 18-19 -0.003186907289226326 1101 20-21 0.04520050338988568 1101 22-23 0.05170717611398601 1101 24-25 -0.0820200381510503 1101 26-27 0.050219280212331796 1101 28-29 0.020396802457852914 1101 30-31 -0.0659146720664765 1101 32-33 -0.1300738882415331 1101 34-35 -0.019217532540118043 1101 36-37 -0.021646631796585325 1101 38-39 0.07009548668220589 1101 40-41 0.005180330784995135 1101 42-43 0.11012999785457112 1101 44-45 -0.04444185161794678 1101 46-47 -0.09477031386902723 1101 48-49 -0.045586647245521306 1101 50-51 1.8231971978366346E-5 1101 52-53 0.09354622672645263 1101 54-55 -0.02546524199718192 1101 56-57 0.07601526977637008 1101 58-59 0.14207776655190685 1101 60-61 0.09930483778598642 1101 62-63 -0.12505297626094602 1101 64-65 -0.05681875406612136 1101 66-67 -0.014050361667855782 1101 68-69 0.03208016787171175 1101 70-71 -0.08341091507774934 1101 72-73 0.03646196654245415 1101 74-75 0.09678748472801857 1101 76-77 -0.4395810523549244 1101 78-79 -0.23901351742276233 1101 80-81 -0.08206812725256185 1101 82-83 -0.2883284404704014 1101 84-85 -0.08064911868530089 1101 86-87 -0.13187221123887838 1101 88-89 -0.31781669152661607 1101 90-91 -0.2679912234429409 1101 92-93 0.021018329068155595 1101 94-95 0.13853265280656757 1101 96-97 -0.037903531309481764 1101 98-99 -0.072237826629582 1101 100 -0.2285066428578446 1102 1 1.740957783752128 1102 2 0.8176442859696067 1102 3 -0.052992095382787596 1102 4 -0.06958354808076628 1102 5 -0.734205956470408 1102 6 -0.04593605104614085 1102 7 0.02383255109479876 1102 8 -0.16929368161997616 1102 9 -0.23269384342329857 1102 10-11 -0.3289063910436951 1102 12-13 -0.38923563322747157 1102 14-15 -0.31259787176519893 1102 16-17 -0.29855870816646046 1102 18-19 -0.3036088784552291 1102 20-21 -0.1172963952093724 1102 22-23 -0.07231946582177073 1102 24-25 -0.24957005200196392 1102 26-27 -0.07277926243042288 1102 28-29 -0.314854922884507 1102 30-31 -0.21459907593509797 1102 32-33 -0.2488736160409175 1102 34-35 -0.24172623452863462 1102 36-37 -0.3654952593514835 1102 38-39 -0.3474074942757923 1102 40-41 -0.3914023471390493 1102 42-43 -0.23460261924589076 1102 44-45 -0.055031960859516005 1102 46-47 -0.5275274290243388 1102 48-49 -0.44129292351709637 1102 50-51 -0.3759799372982542 1102 52-53 -0.3106701727580585 1102 54-55 -0.30273516623869057 1102 56-57 -0.1643009526572925 1102 58-59 -0.05380339258909572 1102 60-61 -0.08989391683599735 1102 62-63 -0.3009495520736394 1102 64-65 -0.29377932111058414 1102 66-67 -0.15570735276713776 1102 68-69 -0.4103457297278652 1102 70-71 -0.49370846888554354 1102 72-73 -0.31239111739409964 1102 74-75 -0.5152101184613045 1102 76-77 -0.2276307831653881 1102 78-79 -0.4943615969631523 1102 80-81 -0.4066601274452708 1102 82-83 -0.39475887876156435 1102 84-85 -0.3203999828620141 1102 86-87 -0.3316333130491316 1102 88-89 -0.5604315035757068 1102 90-91 -0.5908125948040635 1102 92-93 -0.26582384275546644 1102 94-95 -0.28023913227539055 1102 96-97 -0.167296198755011 1102 98-99 -0.40639882245021397 1102 100 -0.6569418506818039 1103 1 1.7252675473031562 1103 2 0.8182245480452544 1103 3 0.26600244251702776 1103 4 0.9269574139148844 1103 5 2.8106926823236478 1103 6 0.0022272035292907333 1103 7 -0.6114759267406953 1103 8 -0.15984911824371295 1103 9 -0.13397680475043217 1103 10-11 -0.3559150555395405 1103 12-13 -0.47339979919619424 1103 14-15 -0.3609694448074876 1103 16-17 -0.38828669232223234 1103 18-19 -0.36953284722248725 1103 20-21 -0.27201492115590753 1103 22-23 -0.2069357604822315 1103 24-25 -0.3086453007885481 1103 26-27 -0.3391145474974664 1103 28-29 -0.45834315552014715 1103 30-31 -0.24945317163390257 1103 32-33 -0.41641117658659965 1103 34-35 -0.49797046790039445 1103 36-37 -0.4214068592097675 1103 38-39 -0.37863272985468654 1103 40-41 -0.43252947022847366 1103 42-43 -0.32598976484472786 1103 44-45 -0.7129988903029059 1103 46-47 -0.6460896810671457 1103 48-49 -0.5160000695666156 1103 50-51 -0.3622175856031049 1103 52-53 -0.5760156321290637 1103 54-55 -0.5795833979729537 1103 56-57 -0.4739729887825135 1103 58-59 -0.36343762091564713 1103 60-61 -0.34277463323118695 1103 62-63 -0.42680739475063234 1103 64-65 -0.46126505242232696 1103 66-67 -0.45161246771160535 1103 68-69 -0.5498794168520575 1103 70-71 -0.7260972332090034 1103 72-73 -0.5149068383024442 1103 74-75 -0.4716558473693695 1103 76-77 -0.6191219002105655 1103 78-79 -0.6079302149879595 1103 80-81 -0.35333867477449843 1103 82-83 -0.4567934015382562 1103 84-85 -0.6375438164976899 1103 86-87 -0.40520616254279673 1103 88-89 -0.472802478203171 1103 90-91 -0.7071235927421569 1103 92-93 -0.46996980589661774 1103 94-95 -0.2903135961119787 1103 96-97 -0.3596814326813842 1103 98-99 -0.4105340817278389 1103 100 -0.4429510873022906 1104 1 1.7713154627908008 1104 2 -0.5675705849485198 1104 3 0.06121089375264255 1104 4 0.04319963236423341 1104 5 -1.0813654199212124 1104 6 -0.6613607987344317 1104 7 -0.48483189731228293 1104 8 -0.3249651340897799 1104 9 -0.11026429767629509 1104 10-11 -0.12545099215527244 1104 12-13 -0.18027208744369716 1104 14-15 -0.2475531082672191 1104 16-17 -0.07640025580082721 1104 18-19 0.00770540971016942 1104 20-21 -0.1217262963681307 1104 22-23 0.0034434132585730026 1104 24-25 -0.14697769090361845 1104 26-27 -0.1754492013363631 1104 28-29 -0.16052576257542484 1104 30-31 -0.113422234074946 1104 32-33 -0.23971998624515578 1104 34-35 -0.1723149312092076 1104 36-37 -0.23243308067740998 1104 38-39 -0.17256029069226742 1104 40-41 -0.18654380714603747 1104 42-43 -0.28977018363363527 1104 44-45 -0.30469291029913137 1104 46-47 -0.4045918504691457 1104 48-49 -0.4167753949769164 1104 50-51 -0.41946150184532627 1104 52-53 -0.3216655095106944 1104 54-55 -0.4009340865222839 1104 56-57 -0.3276477671262299 1104 58-59 -0.18117389708995546 1104 60-61 -0.25343865888672923 1104 62-63 -0.32419090729543143 1104 64-65 -0.24768082303164363 1104 66-67 -0.5393770404337346 1104 68-69 -0.4268460269256238 1104 70-71 -0.3992155127788948 1104 72-73 -0.3014357951030391 1104 74-75 -0.5156777300330262 1104 76-77 -0.6378296911933958 1104 78-79 -0.4696638501511359 1104 80-81 -0.5098902688133577 1104 82-83 -0.442956389653709 1104 84-85 -0.3922976365316444 1104 86-87 -0.414264830718615 1104 88-89 -0.2811197768260669 1104 90-91 -0.35682364933525434 1104 92-93 -0.4586398681490209 1104 94-95 -0.4770783574777582 1104 96-97 -0.77673293239841 1104 98-99 -0.7015753946876515 1104 100 -0.4255241867174959 1105 1 1.4581169034786132 1105 2 0.3263762025500263 1105 3 -0.08591353984791539 1105 4 -0.15668418313477872 1105 5 -1.1500188415547647 1105 6 0.37071309460819535 1105 7 0.05617193677624499 1105 8 -0.022204590346312614 1105 9 0.043238665028482615 1105 10-11 -0.06258704420593375 1105 12-13 -0.04302217458207025 1105 14-15 -0.10760190575762607 1105 16-17 -0.020692111267301527 1105 18-19 -0.018799760500122886 1105 20-21 0.006232341949520048 1105 22-23 -0.06843296643237551 1105 24-25 -0.16144847050162525 1105 26-27 -0.03795994840270822 1105 28-29 -0.02232546049571482 1105 30-31 0.022682099606939232 1105 32-33 -0.07489252022912751 1105 34-35 -0.1126658084021912 1105 36-37 -0.19349384517143164 1105 38-39 -0.18815573625982296 1105 40-41 -0.07383206089298255 1105 42-43 0.1897174427170114 1105 44-45 0.04785036842748269 1105 46-47 0.006880207158367568 1105 48-49 -0.1284728505363475 1105 50-51 -0.11243233953557308 1105 52-53 -0.21539387014738764 1105 54-55 -0.19937024313898632 1105 56-57 0.05078955133549101 1105 58-59 -0.15928008972385754 1105 60-61 -0.10073620739375855 1105 62-63 -0.21874650157915454 1105 64-65 -0.3369941726308099 1105 66-67 -0.19345163088336648 1105 68-69 -0.5254982867141678 1105 70-71 -0.4967872567082594 1105 72-73 -0.2578515032475792 1105 74-75 -0.3755743258271451 1105 76-77 -0.5224828804579502 1105 78-79 -0.42709965821434537 1105 80-81 -0.34709138418457286 1105 82-83 -0.2695161665794714 1105 84-85 -0.2849024930437807 1105 86-87 -0.3971345878929995 1105 88-89 -0.2586171626747955 1105 90-91 -0.31732440453451005 1105 92-93 -0.22044072791432967 1105 94-95 -0.16099493700605194 1105 96-97 -0.2422923607706693 1105 98-99 -0.34676884188179713 1105 100 -0.3893396857299223 1106 1 1.2927374387834139 1106 2 -0.6058438998146336 1106 3 -0.10125448020857597 1106 4 -0.27949196505035445 1106 5 -1.1190847181668246 1106 6 -0.7607236796208596 1106 7 -0.15751887792170294 1106 8 0.03072738668583952 1106 9 -0.11691249712227858 1106 10-11 -0.05889512881270775 1106 12-13 -0.08261280766530632 1106 14-15 -0.05328718028937374 1106 16-17 -0.05470588830313261 1106 18-19 -0.01864038899712739 1106 20-21 -0.10364227051402253 1106 22-23 -0.1213580641190859 1106 24-25 -0.18663604732098094 1106 26-27 -0.2471551108469896 1106 28-29 -0.3146139435541855 1106 30-31 0.027494201751466107 1106 32-33 -0.10596652364127834 1106 34-35 -0.09015888319444088 1106 36-37 -0.3207525635952351 1106 38-39 -0.024183097709808976 1106 40-41 -0.221952394403651 1106 42-43 -0.06805965731784624 1106 44-45 -0.25521460928343487 1106 46-47 -0.33290671658179605 1106 48-49 -0.29271722323176164 1106 50-51 -0.25075189889057015 1106 52-53 -0.2786299601108837 1106 54-55 -0.33598487137916777 1106 56-57 -0.3396029841160768 1106 58-59 -0.29072145018321294 1106 60-61 -0.38581169674453264 1106 62-63 -0.34097068568878797 1106 64-65 -0.5766212662449419 1106 66-67 -0.3432482315694685 1106 68-69 -0.3010034599819562 1106 70-71 -0.49923859680475147 1106 72-73 -0.5207617415481067 1106 74-75 -0.6613102323414282 1106 76-77 -0.5644709654316316 1106 78-79 -0.4436135269747723 1106 80-81 -0.3713376372344115 1106 82-83 -0.411550572479193 1106 84-85 -0.7398622718040357 1106 86-87 -0.6426951169605317 1106 88-89 -0.493335843308067 1106 90-91 -0.5937880999354448 1106 92-93 -0.6995378367547431 1106 94-95 -0.3866397609865331 1106 96-97 -0.40186451134577794 1106 98-99 -0.5300449237735307 1106 100 -0.6687282495060458 1107 1 1.6821547790613458 1107 2 -0.47299937733907704 1107 3 -0.2797726828563558 1107 4 -0.28988037762255203 1107 5 -1.1069589942770932 1107 6 -0.28262497966576205 1107 7 -0.3630660630807512 1107 8 -0.16235310244871926 1107 9 -0.20355427374454393 1107 10-11 -0.1974552913447596 1107 12-13 -0.3354837281386338 1107 14-15 -0.3393593159071031 1107 16-17 -0.2417736337093217 1107 18-19 -0.4067494274869503 1107 20-21 -0.33051686007348025 1107 22-23 -0.11450929565707924 1107 24-25 -0.056247437641168574 1107 26-27 -0.347270815420174 1107 28-29 -0.23630543215896438 1107 30-31 -0.29693886146431225 1107 32-33 -0.10171801324602114 1107 34-35 -0.41846240680552427 1107 36-37 -0.19370901435054577 1107 38-39 -0.2623051404946608 1107 40-41 -0.30138934688918084 1107 42-43 -0.3022448360752179 1107 44-45 -0.15690346097293428 1107 46-47 -0.3478514399148125 1107 48-49 -0.32416610361244125 1107 50-51 -0.497516149049936 1107 52-53 -0.19819424892256876 1107 54-55 -0.38719823700608913 1107 56-57 -0.3146709742241498 1107 58-59 -0.5053264513322091 1107 60-61 -0.1432849598178123 1107 62-63 -0.2533891023665831 1107 64-65 -0.34305048634299595 1107 66-67 -0.346392879232404 1107 68-69 -0.551830650929233 1107 70-71 -0.28110837405391464 1107 72-73 -0.26382379390950206 1107 74-75 -0.31654810277154155 1107 76-77 -0.4654968023938224 1107 78-79 -0.7165332363900774 1107 80-81 -0.3668447254165059 1107 82-83 -0.34530937381490645 1107 84-85 -0.3953646149808847 1107 86-87 -0.4160725607343849 1107 88-89 -0.2483120894368156 1107 90-91 -0.2711621770150927 1107 92-93 -0.2057362101842557 1107 94-95 -0.10934888976126445 1107 96-97 -0.5432045522672411 1107 98-99 -0.4535230005993469 1107 100 -0.45657675740140746 1108 1 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0.2923089775441454 2223 26-27 0.21235826148311787 2223 28-29 0.2107833477638792 2223 30-31 0.06650028796619267 2223 32-33 0.19325623603643294 2223 34-35 0.29782488533723495 2223 36-37 0.2169880610092889 2223 38-39 0.12761692900672728 2223 40-41 0.23651879860692304 2223 42-43 0.34156386214070267 2223 44-45 0.3980749229896716 2223 46-47 0.27082715389455814 2223 48-49 0.16904168579608125 2223 50-51 0.20359487004887455 2223 52-53 0.36157965924876834 2223 54-55 0.2933147334973114 2223 56-57 0.4230486439522281 2223 58-59 0.3687931525137529 2223 60-61 0.36629328518665005 2223 62-63 0.4008175529414473 2223 64-65 0.2759063174082854 2223 66-67 0.46891042951016004 2223 68-69 0.40960423578699334 2223 70-71 0.5594985312143379 2223 72-73 0.4413382720445256 2223 74-75 0.5011795146014038 2223 76-77 0.5791023597461304 2223 78-79 0.5881908118846653 2223 80-81 0.37653505846422775 2223 82-83 0.4396778609474197 2223 84-85 0.3880879721601431 2223 86-87 0.5074005001211788 2223 88-89 0.5662628930465772 2223 90-91 0.5148648358370664 2223 92-93 0.5298773640176861 2223 94-95 0.6494431493349566 2223 96-97 0.652834259693492 2223 98-99 0.6614079520039482 2223 100 0.9092767750778563 2224 1 1.0738502357421371 2224 2 -0.7750988843700597 2224 3 0.003423059237444903 2224 4 -0.3878044180461515 2224 5 -1.0775264385816072 2224 6 0.4031918150274585 2224 7 0.28275460448723777 2224 8 0.20093159826242868 2224 9 0.30438488452090695 2224 10-11 0.32395914456092356 2224 12-13 0.10591577557878651 2224 14-15 0.16721152844304044 2224 16-17 0.15301166262081978 2224 18-19 0.08341886396191 2224 20-21 0.12352165764010437 2224 22-23 0.18192243070740233 2224 24-25 0.14829128722890772 2224 26-27 0.2387526540129059 2224 28-29 0.34548131673422944 2224 30-31 0.1521991653952952 2224 32-33 0.21689655510822803 2224 34-35 0.22450413066348318 2224 36-37 0.30018163145837207 2224 38-39 0.1112242892409796 2224 40-41 0.33746431743081473 2224 42-43 0.4959637008405835 2224 44-45 0.48550153315547817 2224 46-47 0.37021637863441725 2224 48-49 0.3794163877975478 2224 50-51 0.34607734527246947 2224 52-53 0.3529577785673226 2224 54-55 0.43848638408551466 2224 56-57 0.48778738473446737 2224 58-59 0.44147134159918977 2224 60-61 0.35889335598456995 2224 62-63 0.3600744955481687 2224 64-65 0.2887646745986885 2224 66-67 0.19388989467655193 2224 68-69 0.4179145712522825 2224 70-71 0.419245331274368 2224 72-73 0.3773317398480813 2224 74-75 0.39502307411003557 2224 76-77 0.5023855388247753 2224 78-79 0.5345077162055105 2224 80-81 0.4391223100271091 2224 82-83 0.2750245817321044 2224 84-85 0.21492777296366228 2224 86-87 0.49724237331042076 2224 88-89 0.38091279274953393 2224 90-91 0.4570616563460064 2224 92-93 0.34628844790177027 2224 94-95 0.37309280502565656 2224 96-97 0.3334698256816182 2224 98-99 0.47554857637744874 2224 100 0.5858535467156187 2225 1 1.3302767764825756 2225 2 1.4749081278320837 2225 3 0.6469140367566677 2225 4 0.9493609602376392 2225 5 3.196718050796676 2225 6 0.08816287572622272 2225 7 0.07631149670660875 2225 8 0.28494747160768696 2225 9 0.16446226274631925 2225 10-11 0.030217996753513887 2225 12-13 0.12100124577145976 2225 14-15 0.16271049280349814 2225 16-17 0.11612200640572468 2225 18-19 0.09116153233400581 2225 20-21 0.1144773991286101 2225 22-23 0.16569214725252834 2225 24-25 0.14360422869834366 2225 26-27 0.11432306530193159 2225 28-29 0.042016353914867466 2225 30-31 0.15266978107575824 2225 32-33 0.015530053640681274 2225 34-35 0.05523584198514442 2225 36-37 0.1681321164177092 2225 38-39 0.1629601456292562 2225 40-41 0.16673002242621493 2225 42-43 0.4106579243594837 2225 44-45 0.3790062500301232 2225 46-47 0.34295892272520945 2225 48-49 0.2302555032521525 2225 50-51 0.19668842163295608 2225 52-53 0.17904900834339088 2225 54-55 0.17434579387929716 2225 56-57 0.25402359720578715 2225 58-59 0.21868227753319758 2225 60-61 0.23460971229383887 2225 62-63 0.2861223294846269 2225 64-65 0.2892878532903538 2225 66-67 0.3277010117192134 2225 68-69 0.36019964383599046 2225 70-71 0.2819004634196034 2225 72-73 0.4499452594194153 2225 74-75 0.22775605652160635 2225 76-77 0.3765765416335327 2225 78-79 0.329726044254798 2225 80-81 0.28547464228554276 2225 82-83 0.20084357843762035 2225 84-85 0.13133930189054155 2225 86-87 0.23613054134239775 2225 88-89 0.2406780994746569 2225 90-91 0.2851606563261626 2225 92-93 0.16342509554009155 2225 94-95 0.3916946950592717 2225 96-97 0.31185599529300134 2225 98-99 0.4709062135093234 2225 100 0.5315371429286131 2226 1 1.487303478096555 2226 2 1.4223110120480857 2226 3 0.8305849461676313 2226 4 1.07329132249491 2226 5 3.088824458065936 2226 6 -0.17382704665877213 2226 7 0.13148172625280097 2226 8 0.24816893565247966 2226 9 0.14353326206668982 2226 10-11 0.15124455329270603 2226 12-13 0.1566136969762013 2226 14-15 0.1923216300188315 2226 16-17 0.1183972317451989 2226 18-19 0.06368764925965564 2226 20-21 0.149788034727095 2226 22-23 0.22160311291924728 2226 24-25 0.29103941977430736 2226 26-27 0.24966703575843496 2226 28-29 0.26394322883314203 2226 30-31 0.2587241813463237 2226 32-33 0.23561103678567008 2226 34-35 0.10166889667325307 2226 36-37 0.1440794454939862 2226 38-39 0.2198561413343043 2226 40-41 0.1110144918937479 2226 42-43 0.3368614675106869 2226 44-45 0.2297176015772706 2226 46-47 0.40036591431317703 2226 48-49 0.23160270046745524 2226 50-51 0.22994467821387587 2226 52-53 0.3616508575386135 2226 54-55 0.5052986764551193 2226 56-57 0.16903333893109362 2226 58-59 0.274130413068157 2226 60-61 0.2119667270059935 2226 62-63 0.27930017638083626 2226 64-65 0.35370463650196626 2226 66-67 0.2662301359434238 2226 68-69 0.1511681958741775 2226 70-71 0.34636061890834924 2226 72-73 0.45387717868946 2226 74-75 0.44069978783571884 2226 76-77 0.5028890294765844 2226 78-79 0.4403462574223198 2226 80-81 0.3612493600942983 2226 82-83 0.17508743389694104 2226 84-85 0.1212519266767984 2226 86-87 0.48895840226095544 2226 88-89 0.22026026793604814 2226 90-91 0.26802906888003264 2226 92-93 0.299237902409061 2226 94-95 0.3846222417569507 2226 96-97 0.24964530743986302 2226 98-99 0.4749829765490716 2226 100 0.4102039195564231 2227 1 1.385259920239589 2227 2 1.5499462656795888 2227 3 0.752852502049814 2227 4 -0.11539853942514355 2227 5 3.1694301457977403 2227 6 0.30324766049094976 2227 7 0.4014640855459888 2227 8 0.4372701324892603 2227 9 0.38020511910891486 2227 10-11 0.251730646512037 2227 12-13 0.28023550209120884 2227 14-15 0.2542287668790877 2227 16-17 0.2678089500739347 2227 18-19 0.1333304284664294 2227 20-21 0.2333842305478555 2227 22-23 0.37345667768888546 2227 24-25 0.20653948383273502 2227 26-27 0.15249139082141028 2227 28-29 0.2161646720297412 2227 30-31 0.30989525396606155 2227 32-33 0.2706329316294145 2227 34-35 0.3065952247828889 2227 36-37 0.25226750931964403 2227 38-39 0.3467194087273384 2227 40-41 0.26258038033257947 2227 42-43 0.48443350039683253 2227 44-45 0.4239943912595905 2227 46-47 0.3548383192075235 2227 48-49 0.2216588020448853 2227 50-51 0.32456080160402934 2227 52-53 0.32018070712566526 2227 54-55 0.3052154474220572 2227 56-57 0.5103300974805478 2227 58-59 0.5340767166150258 2227 60-61 0.38613573896215314 2227 62-63 0.47407799451777066 2227 64-65 0.39632191388248117 2227 66-67 0.41566338306771655 2227 68-69 0.37968955595906095 2227 70-71 0.48103309025451324 2227 72-73 0.42905312819733865 2227 74-75 0.41487913596021286 2227 76-77 0.7334343309900078 2227 78-79 0.5450653694081922 2227 80-81 0.4472664015145327 2227 82-83 0.5206408518249432 2227 84-85 0.44608068182669314 2227 86-87 0.5245994699578134 2227 88-89 0.5042463754687958 2227 90-91 0.4707256121180663 2227 92-93 0.44647227930822453 2227 94-95 0.5820995479426543 2227 96-97 0.63337438344508 2227 98-99 0.5687240453253111 2227 100 0.5211307160200249 2228 1 1.2907751772268394 2228 2 1.3232728628643926 2228 3 -0.06156165291910298 2228 4 -0.10669060512193163 2228 5 3.1706112124560093 2228 6 0.22660922974939268 2228 7 0.38817269275647703 2228 8 0.600897454128436 2228 9 0.37139175559027393 2228 10-11 0.3375688376674759 2228 12-13 0.39858946664836026 2228 14-15 0.36579292486110404 2228 16-17 0.2890301765364782 2228 18-19 0.26800204063598443 2228 20-21 0.2384631120028402 2228 22-23 0.3416592734882542 2228 24-25 0.3246682025269578 2228 26-27 0.317952166612983 2228 28-29 0.4000219614398972 2228 30-31 0.3056737219474783 2228 32-33 0.3485506645286236 2228 34-35 0.3347661116253846 2228 36-37 0.43284658695080225 2228 38-39 0.4720194368064199 2228 40-41 0.4250487471455955 2228 42-43 0.6132786742919478 2228 44-45 0.5249081347125681 2228 46-47 0.37774600036269135 2228 48-49 0.4286524974013872 2228 50-51 0.3922313808267148 2228 52-53 0.5171026103753675 2228 54-55 0.5322671689431644 2228 56-57 0.5458907586089978 2228 58-59 0.5726311429143962 2228 60-61 0.4369905941839036 2228 62-63 0.3140648665756487 2228 64-65 0.52792758786115 2228 66-67 0.48367236176877526 2228 68-69 0.5409835781107546 2228 70-71 0.5706569449942833 2228 72-73 0.5957450318350368 2228 74-75 0.6555570528911971 2228 76-77 0.6006258323102927 2228 78-79 0.687703845198925 2228 80-81 0.5666998362320896 2228 82-83 0.6300148180967966 2228 84-85 0.6334094699107808 2228 86-87 0.6830696637388343 2228 88-89 0.6533685121486954 2228 90-91 0.5534611889237837 2228 92-93 0.5747344673795567 2228 94-95 0.6720899873761041 2228 96-97 0.5448587147649988 2228 98-99 0.7310238904953081 2228 100 0.8678497859598053 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 33.0 13 49.0 14 174.0 15 569.0 16 2272.0 17 5581.0 18 9156.0 19 11622.0 20 13839.0 21 16127.0 22 18380.0 23 21203.0 24 23664.0 25 26890.0 26 30414.0 27 34310.0 28 38366.0 29 43519.0 30 49666.0 31 57086.0 32 66499.0 33 79827.0 34 98534.0 35 127834.0 36 179942.0 37 296681.0 38 722606.0 39 1272537.0 40 1132.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.159566968047013 22.41699537892727 18.253931870954474 29.169505782071237 2 20.914155607910647 47.795529458050915 12.360008878405557 18.93030605563288 3 35.50579465305962 20.182563586035698 22.72209553173884 21.589546229165848 4 18.298531758540527 18.078492552898066 24.93212276882462 38.69085291973679 5 17.99220689349462 35.576688650065016 26.079940600496478 20.35116385594389 6 17.687575111312505 27.52518691634816 31.189572333425275 23.597665638914062 7 20.495476082587967 26.764869577209506 36.01411969541747 16.72553464478506 8 20.720717670120965 27.722046278419164 34.18946890145396 17.36776715000591 9 21.010604239725758 27.973607608652824 27.76926789865637 23.24652025296505 10-11 19.637914220418455 30.22980983293274 28.71448527818275 21.417790668466054 12-13 24.24780945860751 26.661160555971474 27.239763928838805 21.851266056582215 14-15 21.793224097679182 27.258403232002838 28.689381476811537 22.258991193506443 16-17 21.02271747803302 29.473278842744 28.252535314630205 21.251468364592775 18-19 21.479465059695023 29.467737844280705 29.634244848102764 19.41855224792151 20-21 22.89371256747705 28.7849637003034 29.839708149454275 18.48161558276528 22-23 21.850835090035066 30.197410383584856 28.724351333779897 19.227403192600182 24-25 22.182386889751367 28.233726703179794 26.69299051381063 22.890895893258207 26-27 20.27988506737854 26.521419653059613 29.931596373970603 23.267098905591237 28-29 19.933861833260096 27.983170707118127 30.5169381892118 21.56602927040998 30-31 20.37676024792862 29.9034109118516 28.104656958499696 21.615171881720084 32-33 20.793889633161275 29.939369163481615 28.765077672485123 20.50166353087198 34-35 21.44335621970921 29.14526712537925 29.874724181409828 19.536652473501714 36-37 22.028716532369284 27.90750041865322 28.82789104673155 21.23589200224595 38-39 21.527472507175656 29.201005761456727 28.843192701580183 20.428329029787434 40-41 21.00713876729281 29.544768905881558 29.849590267388237 19.598502059437394 42-43 20.616565938404356 29.820526894030465 29.223930116862846 20.338977050702336 44-45 20.65493370503172 30.144755506521136 28.72068811812916 20.47962267031798 46-47 20.048897384602526 28.050610824810896 28.82964737948468 23.0708444111019 48-49 21.038327861853038 28.130545328221864 30.152686853655897 20.6784399562692 50-51 22.023945117025885 30.48244857953426 28.207976451987864 19.285629851451986 52-53 20.492271216003456 30.255256815239527 28.24271153820402 21.009760430552994 54-55 22.36594596966191 28.425204305246705 28.047467630982815 21.161382094108575 56-57 21.447205229963892 28.61563863140309 29.326524696752394 20.610631441880628 58-59 21.235392793406056 27.864679666312757 29.02557457077969 21.874352969501494 60-61 19.713526727906107 30.247539984155793 29.472583623453925 20.566349664484175 62-63 20.616174369385053 30.986712699179435 27.301387040394516 21.095725891040992 64-65 19.983484723369116 30.124157016722297 28.7948234689315 21.097534790977086 66-67 20.273035777611412 30.02211474053351 29.424317964655817 20.28053151719926 68-69 20.97632700756531 29.888468935931282 29.183207573190433 19.951996483312975 70-71 19.783565521691163 30.68511059832933 29.29247298455416 20.23885089542535 72-73 20.240497803301942 30.461515918672923 28.47175876216557 20.826227515859568 74-75 20.14899437034556 28.993259067536155 30.450218438472753 20.407528123645534 76-77 19.687675464951337 30.3553442314512 28.594276394854013 21.36270390874345 78-79 19.656184123684938 29.40597110307735 28.330755742937075 22.607089030300642 80-81 20.719863777274675 29.39485827858889 28.41325244070077 21.47202550343566 82-83 20.52270393337011 28.044578563970212 29.80772427499113 21.624993227668547 84-85 20.725119685566806 28.84600703337405 28.50735967729225 21.921513603766893 86-87 21.5292570875527 28.896861085937193 28.751379092950863 20.822502733559244 88-89 21.31646735489972 28.21839660743134 29.080976151542615 21.384159886126326 90-91 21.83767521868474 28.262616853500926 28.246178558059814 21.65352936975452 92-93 22.062178006422634 27.8816578174869 29.365152414397734 20.69101176169274 94-95 21.86499541944127 28.28705881338902 28.495123305685805 21.352822461483903 96-97 21.60809318235549 28.68818092714449 28.642436906497498 21.06128898400252 98-99 22.409675568383307 29.07257230387328 28.10460604732259 20.413146080420823 100 22.2065671913787 29.950266460459435 26.89782275700382 20.945343591158043 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2.0 1 11.0 2 18.0 3 23.5 4 31.0 5 38.0 6 42.0 7 39.5 8 44.0 9 63.0 10 81.5 11 96.5 12 116.0 13 149.0 14 183.5 15 220.5 16 300.0 17 390.0 18 565.0 19 926.0 20 1432.0 21 2252.5 22 3587.0 23 5486.0 24 8171.0 25 12181.5 26 17412.0 27 23468.0 28 30137.5 29 37764.5 30 46870.5 31 57191.0 32 68869.0 33 81753.0 34 95647.5 35 110855.0 36 125303.0 37 135266.5 38 142872.0 39 150164.0 40 153927.0 41 154183.5 42 152692.0 43 150804.0 44 149646.0 45 145811.5 46 136115.0 47 125932.5 48 119707.0 49 115607.5 50 111402.0 51 103129.0 52 90150.0 53 76599.0 54 64421.0 55 52935.5 56 41175.0 57 30489.5 58 22130.0 59 15845.0 60 12088.0 61 10158.0 62 8563.5 63 6612.0 64 4923.0 65 4637.0 66 5533.5 67 5196.0 68 3448.5 69 2916.0 70 3511.5 71 3292.0 72 2004.0 73 915.0 74 442.5 75 264.5 76 185.5 77 136.5 78 111.5 79 106.0 80 77.0 81 49.0 82 43.5 83 35.0 84 20.5 85 19.5 86 23.0 87 23.5 88 19.0 89 13.5 90 15.0 91 16.5 92 14.0 93 11.5 94 10.0 95 12.5 96 19.0 97 20.0 98 48.0 99 109.5 100 107.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.361940482288507 2 0.005571781788092517 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.9244158556286697E-4 30-31 0.00981988060995311 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.619330942905551E-4 40-41 0.0016007328893967454 42-43 9.234997438827377E-5 44-45 0.0 46-47 0.0023549243469009815 48-49 1.5391662398045627E-5 50-51 0.0 52-53 0.0011851580046495134 54-55 0.018916353087198075 56-57 0.011774621734504906 58-59 0.015407054060443676 60-61 2.0009161117459317E-4 62-63 0.011482180148942037 64-65 1.3852496158241065E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.616582607667757E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3248512.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.71708161513762 #Duplication Level Percentage of deduplicated Percentage of total 1 86.92043568576867 65.81361722842665 2 8.343098225455622 12.634300985198662 3 2.2346159331429902 5.07595790964824 4 0.8894751108001078 2.6939383823634135 5 0.45927861999445346 1.7387618377103904 6 0.27388650416440863 1.2442732073460754 7 0.1814837995119766 0.9618996561631523 8 0.1263473654755572 0.7653323026856308 9 0.09526371688589948 0.6491781563769913 >10 0.4436907726699368 5.816551873712426 >50 0.023288534345510335 1.2048162066018757 >100 0.008683848564788666 1.1753958909852338 >500 4.5188322026599163E-4 0.22597636278117544 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 1.5391662398045627E-5 0.0 0.0 0.0 1.5391662398045627E-5 12-13 3.0783324796091254E-5 0.0 0.0 0.0 3.0783324796091254E-5 14-15 3.0783324796091254E-5 0.0 0.0 0.0 3.0783324796091254E-5 16-17 3.0783324796091254E-5 0.0 0.0 0.0 3.0783324796091254E-5 18-19 4.6174987194136884E-5 0.0 0.0 0.0 3.0783324796091254E-5 20-21 6.156664959218251E-5 0.0 0.0 0.0 3.0783324796091254E-5 22-23 6.156664959218251E-5 1.5391662398045627E-5 0.0 0.0 3.0783324796091254E-5 24-25 6.156664959218251E-5 3.0783324796091254E-5 0.0 0.0 3.0783324796091254E-5 26-27 6.156664959218251E-5 3.0783324796091254E-5 0.0 0.0 3.0783324796091254E-5 28-29 6.156664959218251E-5 3.0783324796091254E-5 0.0 0.0 3.0783324796091254E-5 30-31 6.156664959218251E-5 3.0783324796091254E-5 0.0 0.0 3.0783324796091254E-5 32-33 6.156664959218251E-5 3.0783324796091254E-5 0.0 0.0 3.0783324796091254E-5 34-35 6.156664959218251E-5 3.0783324796091254E-5 0.0 0.0 3.0783324796091254E-5 36-37 6.156664959218251E-5 3.0783324796091254E-5 0.0 0.0 3.0783324796091254E-5 38-39 7.695831199022813E-5 3.0783324796091254E-5 0.0 0.0 3.0783324796091254E-5 40-41 9.234997438827377E-5 3.0783324796091254E-5 0.0 0.0 3.0783324796091254E-5 42-43 9.234997438827377E-5 3.0783324796091254E-5 0.0 0.0 3.0783324796091254E-5 44-45 9.234997438827377E-5 3.0783324796091254E-5 0.0 0.0 3.0783324796091254E-5 46-47 9.234997438827377E-5 3.0783324796091254E-5 0.0 0.0 3.0783324796091254E-5 48-49 9.234997438827377E-5 3.0783324796091254E-5 0.0 0.0 3.0783324796091254E-5 50-51 9.234997438827377E-5 3.0783324796091254E-5 0.0 0.0 3.0783324796091254E-5 52-53 9.234997438827377E-5 3.0783324796091254E-5 0.0 0.0 3.0783324796091254E-5 54-55 1.0774163678631939E-4 3.0783324796091254E-5 0.0 0.0 3.0783324796091254E-5 56-57 1.2313329918436501E-4 3.0783324796091254E-5 0.0 0.0 3.0783324796091254E-5 58-59 1.3852496158241065E-4 3.0783324796091254E-5 0.0 0.0 3.0783324796091254E-5 60-61 2.1548327357263878E-4 3.0783324796091254E-5 0.0 0.0 3.0783324796091254E-5 62-63 3.078332479609125E-4 3.0783324796091254E-5 0.0 0.0 3.0783324796091254E-5 64-65 4.0018322234918635E-4 3.0783324796091254E-5 0.0 0.0 3.0783324796091254E-5 66-67 4.1557488474723195E-4 3.0783324796091254E-5 0.0 0.0 3.0783324796091254E-5 68-69 5.079248591355057E-4 3.0783324796091254E-5 0.0 0.0 3.0783324796091254E-5 70-71 5.233165215335514E-4 3.0783324796091254E-5 0.0 0.0 3.0783324796091254E-5 72-73 6.156664959218252E-4 3.0783324796091254E-5 0.0 0.0 3.0783324796091254E-5 74-75 6.772331455140076E-4 3.0783324796091254E-5 0.0 0.0 3.0783324796091254E-5 76-77 7.849747823003271E-4 3.0783324796091254E-5 0.0 0.0 3.0783324796091254E-5 78-79 0.0010004580558729658 3.0783324796091254E-5 0.0 0.0 3.0783324796091254E-5 80-81 0.001492991252610426 3.0783324796091254E-5 0.0 0.0 3.0783324796091254E-5 82-83 0.0016469078765908823 3.0783324796091254E-5 0.0 0.0 3.0783324796091254E-5 84-85 0.001785432838173293 3.0783324796091254E-5 0.0 0.0 3.0783324796091254E-5 86-87 0.0018777828125615666 3.0783324796091254E-5 0.0 0.0 3.0783324796091254E-5 88 0.0018777828125615666 3.0783324796091254E-5 0.0 0.0 3.0783324796091254E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 12065 0.0 80.53017 8 CACCGGC 12040 0.0 76.95489 9 CTTCACC 13705 0.0 74.38688 6 CCTTCAC 15375 0.0 70.00104 5 GTGTGGC 5485 0.0 68.71546 7 CGTAGTG 2620 0.0 66.28511 4 GGCGCGC 590 0.0 66.03005 6 TTCACCG 15395 0.0 65.27585 7 CGGGGCA 1595 0.0 62.09224 94 ACCTTCA 6250 0.0 61.656475 4 TGTGGCA 6360 0.0 60.81149 8 GGGCCCG 770 0.0 58.518887 8 GGCCCGA 775 0.0 57.535713 9 AGGCGCG 695 0.0 57.404995 5 TGTGTGG 6175 0.0 57.312576 6 CGGGCCC 780 0.0 57.16689 7 GCCTTCA 4515 0.0 56.553135 4 GCGCGCA 695 0.0 56.054287 7 ATGTGTG 6110 0.0 54.849487 5 GCGTAGT 1640 0.0 54.664463 3 >>END_MODULE