FastQCFastQC Report
Tue 7 Mar 2017
1SN_19A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_19A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3036463
Sequences flagged as poor quality0
Sequence length94
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA150770.49653165541618655No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG94470.31111856129977544No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA68300.22493275893696055No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG62960.207346508091816No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT60160.19812525296702116No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA56620.18646695184495907No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG52690.17352426161622914No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG52240.17204227418545853No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC43600.14358811551466294No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC42090.13861522435807716No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA42030.1384176260339744No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT41870.1378906971697004No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA41250.13584884782063866No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT41100.1353548520103818No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG40600.1337081993095256No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC39450.12992089809755628No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA39450.12992089809755628No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA35580.11717580619292908No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG35310.11628661373446672No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA34000.1119723836582234No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA32430.10680189417753484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTGCC1300.087.999772
AGCAGCC900.087.999772
GCTGCTT257.290764E-787.999763
GCAGCTA302.0971129E-887.999763
AGCAGCG1550.087.999762
CAGCTGC4200.084.856921
AGCTGCA1300.084.6151662
CAGCAGC4350.083.95381
GCTGCCC900.083.110893
CAGCCCT166100.082.039521
AGCAGCA1350.081.481272
CAGCGTA65900.080.855631
GCTGCAT550.079.9997943
AGCCCTT145400.079.435842
CTGCAGC950.078.736641
AGCTGCG900.078.2220152
GCCCTTC132250.077.819083
CAGCTAG51200.077.773231
GCTGCAA850.077.646863
CAGCACT76500.076.5540541
Produced by FastQC (version 0.11.5)