##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1SN_19A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3036463
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.817329241291596	12.0	12.0	12.0	2.0	32.0
2	14.647093015788435	12.0	12.0	12.0	12.0	22.0
3	13.694150398012424	12.0	12.0	12.0	12.0	22.0
4	13.793318410268789	12.0	12.0	12.0	12.0	22.0
5	22.459839293283007	22.0	22.0	22.0	22.0	27.0
6	26.871087512016448	27.0	22.0	32.0	22.0	32.0
7	35.03729470769115	37.0	37.0	37.0	27.0	41.0
8	37.66408581300019	41.0	37.0	41.0	32.0	41.0
9	38.60459159225717	41.0	41.0	41.0	32.0	41.0
10-11	38.955720026886546	41.0	41.0	41.0	37.0	41.0
12-13	39.13479614274898	41.0	41.0	41.0	37.0	41.0
14-15	39.09303637159419	41.0	41.0	41.0	37.0	41.0
16-17	39.003563521109925	41.0	41.0	41.0	37.0	41.0
18-19	38.93820952206564	41.0	41.0	41.0	37.0	41.0
20-21	38.952779928489164	41.0	41.0	41.0	37.0	41.0
22-23	38.9370114175605	41.0	41.0	41.0	37.0	41.0
24-25	38.95697757555419	41.0	41.0	41.0	37.0	41.0
26-27	38.93338137168146	41.0	41.0	41.0	37.0	41.0
28-29	38.8784396516605	41.0	41.0	41.0	37.0	41.0
30-31	38.74418443432375	41.0	41.0	41.0	32.0	41.0
32-33	38.79828949010741	41.0	41.0	41.0	37.0	41.0
34-35	38.77297335748863	41.0	41.0	41.0	32.0	41.0
36-37	38.700858367119906	41.0	41.0	41.0	32.0	41.0
38-39	38.61108697850098	41.0	41.0	41.0	32.0	41.0
40-41	38.65494343253977	41.0	41.0	41.0	32.0	41.0
42-43	38.390898719990986	41.0	41.0	41.0	32.0	41.0
44-45	38.4960780025971	41.0	41.0	41.0	32.0	41.0
46-47	38.52244140633361	41.0	41.0	41.0	32.0	41.0
48-49	38.45960843257434	41.0	41.0	41.0	32.0	41.0
50-51	38.35067873377676	41.0	41.0	41.0	32.0	41.0
52-53	38.32643835936746	41.0	41.0	41.0	32.0	41.0
54-55	38.20341726541703	41.0	41.0	41.0	32.0	41.0
56-57	37.9860393490716	41.0	37.0	41.0	32.0	41.0
58-59	37.9937985412633	41.0	37.0	41.0	29.5	41.0
60-61	37.99617614309807	41.0	37.0	41.0	32.0	41.0
62-63	37.9917063043416	41.0	37.0	41.0	32.0	41.0
64-65	37.967444852777724	41.0	37.0	41.0	32.0	41.0
66-67	37.856008125243086	41.0	37.0	41.0	32.0	41.0
68-69	37.87031836053988	41.0	37.0	41.0	32.0	41.0
70-71	37.84984371619216	41.0	37.0	41.0	29.5	41.0
72-73	37.71084333976735	41.0	37.0	41.0	27.0	41.0
74-75	37.6138176226748	41.0	37.0	41.0	27.0	41.0
76-77	36.59111653921026	39.0	34.5	41.0	24.5	41.0
78-79	37.10869439212663	41.0	37.0	41.0	27.0	41.0
80-81	37.742498261958076	41.0	37.0	41.0	29.5	41.0
82-83	37.932279925689855	41.0	37.0	41.0	32.0	41.0
84-85	37.844524698637855	41.0	37.0	41.0	29.5	41.0
86-87	37.69795630639992	41.0	37.0	41.0	27.0	41.0
88-89	37.6268760725884	41.0	37.0	41.0	27.0	41.0
90-91	37.47762660042292	41.0	37.0	41.0	27.0	41.0
92-93	37.37383314072986	41.0	37.0	41.0	27.0	41.0
94-95	37.21017611609297	41.0	37.0	41.0	27.0	41.0
96-97	37.15376344121434	41.0	37.0	41.0	27.0	41.0
98-99	37.02982680836223	41.0	37.0	41.0	27.0	41.0
100	36.90071112343539	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.3047615888174082
1101	2	-0.361696921183972
1101	3	0.04520881180685876
1101	4	0.07479605619475471
1101	5	-1.0423908680000729
1101	6	-0.9460824269187675
1101	7	-0.3616323756232305
1101	8	-0.2852257347830829
1101	9	-0.17291513474520315
1101	10-11	-0.05014562246429932
1101	12-13	-0.013327722621802707
1101	14-15	-0.08443974995545744
1101	16-17	-0.06924926264171205
1101	18-19	-0.028136913728985746
1101	20-21	-0.15871586535551074
1101	22-23	0.04999723303774317
1101	24-25	-0.021506075996839513
1101	26-27	-0.05678135225695513
1101	28-29	0.011407043799522398
1101	30-31	-0.132500323281306
1101	32-33	-0.12693412404390614
1101	34-35	0.14167345832808564
1101	36-37	0.1115213250317737
1101	38-39	0.0076112788382047825
1101	40-41	-0.11195251198570588
1101	42-43	0.022409682131012687
1101	44-45	-0.09025901127798619
1101	46-47	-0.04348207561922379
1101	48-49	0.09166990346363946
1101	50-51	-0.01828508009823082
1101	52-53	0.062110526818599965
1101	54-55	-0.03754485627139559
1101	56-57	0.12490116882973723
1101	58-59	-0.07950348855868583
1101	60-61	-0.054418866577499614
1101	62-63	-0.09487008205486092
1101	64-65	-0.09882337863459156
1101	66-67	-0.042211028116931004
1101	68-69	0.013813761443756789
1101	70-71	-0.18825716062747233
1101	72-73	0.052644761336260615
1101	74-75	0.04802028496383315
1101	76-77	-0.2552685013556868
1101	78-79	-0.04721183477097668
1101	80-81	0.04763360820429341
1101	82-83	-0.15382310626421258
1101	84-85	-0.1129704739773274
1101	86-87	-0.06023629384022655
1101	88-89	-0.19404386450646882
1101	90-91	-0.27798858283284744
1101	92-93	-0.054848873062304904
1101	94-95	0.02155479909264102
1101	96-97	-0.1150620108319842
1101	98-99	-0.2946171235852759
1101	100	-0.3306548096719979
1102	1	1.7989717396205194
1102	2	0.7510486745390779
1102	3	-0.09278653461749542
1102	4	-0.0033627356001542097
1102	5	-0.7607118534566055
1102	6	-0.14352685451922653
1102	7	-0.23715854537999093
1102	8	-0.17939165853731964
1102	9	-0.08619743544184644
1102	10-11	-0.17814982820631542
1102	12-13	-0.20867873657557112
1102	14-15	-0.13693816098050604
1102	16-17	-0.2965638463024547
1102	18-19	-0.31560202234254575
1102	20-21	-0.05744934453611705
1102	22-23	-0.030154905228812368
1102	24-25	0.013409162446755829
1102	26-27	-0.06545291356913907
1102	28-29	-0.28861061920427744
1102	30-31	-0.22884449873195933
1102	32-33	-0.28074947194249233
1102	34-35	-0.1040442368029062
1102	36-37	-0.17870171559174963
1102	38-39	-0.04872626255511392
1102	40-41	-0.04606450099292658
1102	42-43	-0.0960140129971947
1102	44-45	0.039491328421910055
1102	46-47	-0.4345206046108814
1102	48-49	-0.35942172580243437
1102	50-51	-0.1804768641589689
1102	52-53	-0.09429328560864292
1102	54-55	-0.09314687958934087
1102	56-57	-0.042030141556523404
1102	58-59	-0.011479227658035995
1102	60-61	-0.025260098425981425
1102	62-63	-0.14578250832573048
1102	64-65	-0.28169096034684316
1102	66-67	-0.12864556304447206
1102	68-69	-0.20491799316009462
1102	70-71	-0.450943300176327
1102	72-73	-0.33745888016317593
1102	74-75	-0.3077578816163751
1102	76-77	-0.22812060360083564
1102	78-79	-0.20054798676737562
1102	80-81	-0.19065498580668816
1102	82-83	-0.2503487795097641
1102	84-85	-0.21991314709909915
1102	86-87	-0.09705246091276365
1102	88-89	-0.20929586828385283
1102	90-91	-0.1707784612362815
1102	92-93	-0.2852862618862204
1102	94-95	-0.056721444723571324
1102	96-97	-0.06359236546894209
1102	98-99	-0.2553944765546774
1102	100	-0.5397448590032354
1103	1	1.4077250926378913
1103	2	0.8034839783713963
1103	3	0.4187923473512356
1103	4	1.0447075952260771
1103	5	2.9317533304292596
1103	6	-0.2081928965174633
1103	7	-0.49968212396431966
1103	8	-0.18592900390840583
1103	9	-0.06272541078647009
1103	10-11	-0.09888778461582604
1103	12-13	-0.34085780594482884
1103	14-15	-0.3488676423202861
1103	16-17	-0.11142064816866082
1103	18-19	-0.12265793402011127
1103	20-21	-0.07104007305025561
1103	22-23	-0.15036974396971203
1103	24-25	-0.20978905804289383
1103	26-27	-0.12361932148244392
1103	28-29	-0.11464462727101932
1103	30-31	-0.07629887855107143
1103	32-33	-0.29556935720482613
1103	34-35	-0.2977508588903106
1103	36-37	-0.2052647653222408
1103	38-39	-0.10180511454264973
1103	40-41	-0.31741981716449175
1103	42-43	-0.1021397851935788
1103	44-45	-0.4802515412913735
1103	46-47	-0.2989270022366668
1103	48-49	-0.31624923856972487
1103	50-51	-0.14941367319176635
1103	52-53	-0.09910617044256753
1103	54-55	-0.2153329638357775
1103	56-57	-0.2504802871639882
1103	58-59	-0.41289224498716237
1103	60-61	-0.2373161509790478
1103	62-63	-0.013725675539156157
1103	64-65	-0.259312201770598
1103	66-67	-0.27148666776842134
1103	68-69	-0.2834515298357658
1103	70-71	-0.4028802659782613
1103	72-73	-0.30751727486163105
1103	74-75	-0.400428743723829
1103	76-77	-0.3214198426564323
1103	78-79	-0.47108933155762145
1103	80-81	-0.2879628189898824
1103	82-83	-0.1692877197359337
1103	84-85	-0.3684776980566511
1103	86-87	-0.23238858622983116
1103	88-89	-0.22006229599196558
1103	90-91	-0.5245993297373133
1103	92-93	-0.40706973119450396
1103	94-95	-0.41081734553565497
1103	96-97	-0.04804614060709156
1103	98-99	-0.21104325877632846
1103	100	-0.36012614755887284
1104	1	1.6981052266053158
1104	2	-0.603036710573539
1104	3	0.057511068797852616
1104	4	0.13844972137358447
1104	5	-1.003617728979311
1104	6	-0.4945186207897194
1104	7	-0.01765811573016407
1104	8	-0.03888881066277605
1104	9	0.15747669739733539
1104	10-11	0.038953736661710536
1104	12-13	-0.02555637636583441
1104	14-15	-0.16766004973096926
1104	16-17	0.020776708789718157
1104	18-19	0.13450523254169866
1104	20-21	0.01052560061087604
1104	22-23	0.05229339147398804
1104	24-25	-0.02509817731696984
1104	26-27	0.028188338977422234
1104	28-29	0.048905269435536525
1104	30-31	-0.050508934861198895
1104	32-33	0.021974444951247563
1104	34-35	-0.07562172749382512
1104	36-37	0.0604117560839299
1104	38-39	-0.0752515194520953
1104	40-41	-0.004746754108850837
1104	42-43	0.015692071502705573
1104	44-45	0.08328955897894019
1104	46-47	-0.13954614198831905
1104	48-49	-0.2722495220104548
1104	50-51	-0.08156152863642063
1104	52-53	-0.05498435302409632
1104	54-55	-0.01381832387807691
1104	56-57	0.02052519292954713
1104	58-59	0.028162289611643132
1104	60-61	-0.1359909793587022
1104	62-63	0.12121065019229604
1104	64-65	-0.011290717193752187
1104	66-67	-0.3565652384855227
1104	68-69	-0.3448114876243693
1104	70-71	-0.20305929932752775
1104	72-73	-0.025888588820670577
1104	74-75	0.14248873808644902
1104	76-77	-0.013295198304831501
1104	78-79	-0.09496456992020086
1104	80-81	-0.21703709612620514
1104	82-83	-0.14772718020392972
1104	84-85	-0.03334490420932923
1104	86-87	-0.08542306207937145
1104	88-89	-0.1484059083634932
1104	90-91	-0.19722479142444627
1104	92-93	-0.17206467252500346
1104	94-95	-0.13643062746445622
1104	96-97	-0.04810277125867657
1104	98-99	-0.08956504928805487
1104	100	-0.23479446657572112
1105	1	1.4402489089665345
1105	2	0.2837822271593726
1105	3	-0.03127414055950872
1105	4	-0.15525382841067525
1105	5	-1.0820004101756702
1105	6	0.5688918989750178
1105	7	0.13659218776789572
1105	8	0.03195592730529029
1105	9	-0.12458317241411265
1105	10-11	-0.06275595200627038
1105	12-13	-0.14380706746793948
1105	14-15	-0.02250295067424446
1105	16-17	-0.04728533242323607
1105	18-19	-0.02577142666632426
1105	20-21	-0.051590335306173074
1105	22-23	-0.01684779943942516
1105	24-25	-0.055441227364973145
1105	26-27	-0.1898314957039915
1105	28-29	-0.08549349732851397
1105	30-31	-0.013559351364946792
1105	32-33	-0.2712478753093208
1105	34-35	-0.10963125245368133
1105	36-37	-0.22132513310703672
1105	38-39	0.06359336591469145
1105	40-41	-0.03986661451679652
1105	42-43	0.12530335514765767
1105	44-45	-0.05349686232344908
1105	46-47	-0.11909723099871883
1105	48-49	-0.1919000915086002
1105	50-51	-0.09438146404028913
1105	52-53	-0.2683177273015218
1105	54-55	-0.1393604065537417
1105	56-57	0.06434232844014787
1105	58-59	-0.017330005052045294
1105	60-61	-0.1333917803615705
1105	62-63	-0.05515239558388174
1105	64-65	-0.13671426673234066
1105	66-67	-0.24308214532339179
1105	68-69	-0.2162062411853114
1105	70-71	-0.3890031936426439
1105	72-73	-0.19797659404513723
1105	74-75	-0.33826849553983607
1105	76-77	-0.3171201268403365
1105	78-79	-0.1838058044390749
1105	80-81	-0.2337585937517872
1105	82-83	-0.16785652258045047
1105	84-85	-0.05132754801009298
1105	86-87	-0.17720756242160007
1105	88-89	-0.08841067152582127
1105	90-91	-0.136134436691691
1105	92-93	-0.04338381600196328
1105	94-95	0.027847292821498115
1105	96-97	-0.07219086110092832
1105	98-99	-0.2891110475834182
1105	100	-0.13798599005749423
1106	1	0.9764575284174892
1106	2	-0.7470100954136196
1106	3	-0.23713500185023229
1106	4	-0.24402629350057659
1106	5	-1.1223199894140627
1106	6	-0.5165751663136504
1106	7	-0.1865644240541613
1106	8	-0.139498156866118
1106	9	-0.08311735043346147
1106	10-11	-0.15809422857958566
1106	12-13	-0.09763576089026316
1106	14-15	-0.027935470540590757
1106	16-17	-0.12671083414301165
1106	18-19	-0.05666054185908109
1106	20-21	-0.12737895658391096
1106	22-23	-0.07091758828156003
1106	24-25	-0.162984780635675
1106	26-27	-0.17516039293614227
1106	28-29	-0.07014396009266477
1106	30-31	0.04155436633051579
1106	32-33	-0.059087723058617314
1106	34-35	-0.24698270645606613
1106	36-37	-0.1682632144591878
1106	38-39	0.009145267889671516
1106	40-41	-0.08118663409344862
1106	42-43	-0.0090072428262431
1106	44-45	-0.11890052162978293
1106	46-47	-0.22461536461888443
1106	48-49	-0.24804206037955367
1106	50-51	-0.2586061619723452
1106	52-53	-0.30136831712302836
1106	54-55	-0.3206993347731597
1106	56-57	-0.24198865869460917
1106	58-59	-0.22559466334001854
1106	60-61	-0.10824764797107633
1106	62-63	-0.16824257495014905
1106	64-65	-0.2879006579626662
1106	66-67	-0.17087539628756332
1106	68-69	-0.35770028298608736
1106	70-71	-0.3577590812923219
1106	72-73	-0.3394870633257483
1106	74-75	-0.33878265520949213
1106	76-77	-0.20017521547374884
1106	78-79	-0.22827022088181792
1106	80-81	-0.2118417454285435
1106	82-83	-0.4414855778163158
1106	84-85	-0.4747768552612257
1106	86-87	-0.38103196152347607
1106	88-89	-0.34282864114384637
1106	90-91	-0.4318827867865025
1106	92-93	-0.40681201973543324
1106	94-95	-0.19485650676598
1106	96-97	-0.2314590270989001
1106	98-99	-0.5945238681067195
1106	100	-0.4107804475212262
1107	1	1.4284213301205693
1107	2	-0.49288746818485585
1107	3	-0.21485852571971975
1107	4	-0.30932913301171006
1107	5	-1.1491371604411356
1107	6	-0.3093777044141852
1107	7	-0.5666791945132488
1107	8	-0.2304008034744669
1107	9	-0.2361220234141186
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2222	28-29	0.15248297228602326
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2222	58-59	0.5265109729533819
2222	60-61	0.5752730935265404
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2223	4	-0.11683714739701045
2223	5	-1.138340231031357
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2223	9	0.10988150818153741
2223	10-11	0.009378539537266306
2223	12-13	0.06906369634386778
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2223	34-35	0.1521775544366193
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2223	40-41	0.176810848683985
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2223	44-45	0.27747670816295766
2223	46-47	0.1467268817371945
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2223	50-51	0.25083308106834323
2223	52-53	0.21549450545076354
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2223	66-67	0.27950509725965844
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2223	82-83	0.13210656600092818
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2223	86-87	0.5102586998048935
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2223	94-95	0.4730221129369454
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2223	100	0.6013213212839545
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2224	3	-0.024560278158135773
2224	4	-0.3101929996078532
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2224	9	0.3208508628743729
2224	10-11	0.19759298542516035
2224	12-13	0.18305655599755966
2224	14-15	0.18977059851544453
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2224	18-19	0.182321093454334
2224	20-21	0.14806084687986498
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2224	24-25	0.1664740210040776
2224	26-27	0.1571773699436605
2224	28-29	0.02965379496406939
2224	30-31	0.20719029127788247
2224	32-33	0.21724538989522557
2224	34-35	0.17259219695510097
2224	36-37	0.18405751983552676
2224	38-39	0.06795182755436713
2224	40-41	0.14436913289630127
2224	42-43	0.15351633005390397
2224	44-45	0.3311735616606555
2224	46-47	0.27658045841720025
2224	48-49	0.036069594768015634
2224	50-51	0.08037632720995447
2224	52-53	0.18029627179602414
2224	54-55	0.24888000062409077
2224	56-57	0.30787830040181774
2224	58-59	0.36937364999245403
2224	60-61	0.2168926101575508
2224	62-63	0.3583879689968583
2224	64-65	0.33482754256153413
2224	66-67	0.322999924218216
2224	68-69	0.2956370838611875
2224	70-71	0.4508063620648599
2224	72-73	0.4265990131630062
2224	74-75	0.30134840959753006
2224	76-77	0.5346535675516648
2224	78-79	0.37155010070959804
2224	80-81	0.2314959223058608
2224	82-83	0.16592584141947953
2224	84-85	0.10457155333048718
2224	86-87	0.38631316495890644
2224	88-89	0.19961209832286642
2224	90-91	0.26371092450985856
2224	92-93	0.23588980030641693
2224	94-95	0.5006987804307883
2224	96-97	0.34601737562469737
2224	98-99	0.2693265356919383
2224	100	0.4021137044527947
2225	1	1.079028291553156
2225	2	1.6532831872232485
2225	3	1.012012377024556
2225	4	1.1596864450192488
2225	5	3.2490246821150954
2225	6	0.011258274812199431
2225	7	0.24477595752318138
2225	8	0.3489598881936047
2225	9	0.3578063702542451
2225	10-11	0.21207451224343288
2225	12-13	0.14150132264391146
2225	14-15	0.18419856843272697
2225	16-17	0.2093243124697608
2225	18-19	0.14985061541386813
2225	20-21	0.11442140406565215
2225	22-23	0.2447932999586726
2225	24-25	0.17960960170233164
2225	26-27	0.19990991687287618
2225	28-29	0.13858054589002222
2225	30-31	0.32371412054727244
2225	32-33	0.17495062497826552
2225	34-35	0.23003274851670596
2225	36-37	0.1994199777651815
2225	38-39	0.1673101227449436
2225	40-41	0.20709520486589383
2225	42-43	0.48852134529841607
2225	44-45	0.26562970282060405
2225	46-47	0.3946667725709432
2225	48-49	0.2314766140960245
2225	50-51	0.24461938972008568
2225	52-53	0.2156664344770718
2225	54-55	0.2627630641254868
2225	56-57	0.4086674211974497
2225	58-59	0.5910042557987438
2225	60-61	0.37775155874125943
2225	62-63	0.2923046847955604
2225	64-65	0.38583613745421275
2225	66-67	0.4015208027825068
2225	68-69	0.40621100037555635
2225	70-71	0.34793010707760885
2225	72-73	0.48170030997047064
2225	74-75	0.41466371481978825
2225	76-77	0.5827576407995494
2225	78-79	0.5422758753028134
2225	80-81	0.2376076427911613
2225	82-83	0.34102705076330153
2225	84-85	0.2285445678827216
2225	86-87	0.3520934804190432
2225	88-89	0.43607905502425837
2225	90-91	0.250390278704856
2225	92-93	0.3579617396787427
2225	94-95	0.45663228050161564
2225	96-97	0.4954341588101556
2225	98-99	0.37371465267004567
2225	100	0.4270548884692644
2226	1	1.5686650800933908
2226	2	1.5180905757273209
2226	3	0.9267852020752354
2226	4	1.029874268464205
2226	5	3.1035040712566904
2226	6	-0.3238183220895081
2226	7	0.05453942489282326
2226	8	0.23603614832998687
2226	9	0.3067755692002123
2226	10-11	0.17418755313633483
2226	12-13	0.17726761333539542
2226	14-15	0.13718128458800294
2226	16-17	0.15384143150597396
2226	18-19	0.17111918549295524
2226	20-21	0.16375706033839066
2226	22-23	0.20178642248009737
2226	24-25	0.3195653112380157
2226	26-27	0.23968707724755944
2226	28-29	0.16627969214227534
2226	30-31	0.21286954912747547
2226	32-33	0.239396970346462
2226	34-35	0.14614528796815307
2226	36-37	0.19180000048624635
2226	38-39	0.20082131688246818
2226	40-41	0.16617047429800635
2226	42-43	0.27963941146062865
2226	44-45	0.2521911912174275
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2226	50-51	0.2816038071756495
2226	52-53	0.31791151014468966
2226	54-55	0.3228295408586277
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2226	60-61	0.24516020783841697
2226	62-63	0.21785598577665866
2226	64-65	0.23355440678757589
2226	66-67	0.36656617740877806
2226	68-69	0.29545948949859735
2226	70-71	0.44105094731768446
2226	72-73	0.3520062130800099
2226	74-75	0.5047810690622256
2226	76-77	0.5530428005420092
2226	78-79	0.3917297303788274
2226	80-81	0.3676935735461697
2226	82-83	0.2985326019636787
2226	84-85	0.18197378931677122
2226	86-87	0.2783835073735048
2226	88-89	0.25299415230363564
2226	90-91	0.22531747835559202
2226	92-93	0.16714751685076834
2226	94-95	0.3047621077690721
2226	96-97	0.39814379588462856
2226	98-99	0.48796261890667836
2226	100	0.5539273844059736
2227	1	1.1746246435524856
2227	2	1.9344510949533777
2227	3	0.4964833020135
2227	4	-0.13823762755825264
2227	5	3.2335293581014994
2227	6	-0.06048643478591487
2227	7	0.19465036899016752
2227	8	0.24388446141090014
2227	9	0.24616771379038482
2227	10-11	0.21207966864582772
2227	12-13	0.19026200476763933
2227	14-15	0.3100985615485925
2227	16-17	0.1674031628357895
2227	18-19	0.14682655282773283
2227	20-21	0.16170353802871773
2227	22-23	0.30824129114483867
2227	24-25	0.3048785047386673
2227	26-27	0.19670464357417217
2227	28-29	0.20210494558433822
2227	30-31	0.19530202193971036
2227	32-33	0.21391963379451084
2227	34-35	0.20782472611890057
2227	36-37	0.282654022449897
2227	38-39	0.09296350589403346
2227	40-41	0.20526970448726445
2227	42-43	0.44026063330773724
2227	44-45	0.31439741640213725
2227	46-47	0.23901212777742842
2227	48-49	0.2636615384897709
2227	50-51	0.316326129446054
2227	52-53	0.33168267567134535
2227	54-55	0.29773452176409165
2227	56-57	0.2637688308908963
2227	58-59	0.33507211619712507
2227	60-61	0.15803638233738582
2227	62-63	0.19138176912725413
2227	64-65	0.2568554952584492
2227	66-67	0.41319556745636277
2227	68-69	0.18109921358971803
2227	70-71	0.32640346642421747
2227	72-73	0.27639433173128936
2227	74-75	0.3717191197607761
2227	76-77	0.5542868663507647
2227	78-79	0.29964631633995964
2227	80-81	0.27416533034424617
2227	82-83	0.21787642461533352
2227	84-85	0.2779358713221285
2227	86-87	0.2038382730553252
2227	88-89	0.26538345232386007
2227	90-91	0.4073958339583186
2227	92-93	0.24876895907067365
2227	94-95	0.41799957626723483
2227	96-97	0.2389112428204072
2227	98-99	0.41038832579377527
2227	100	0.4236969320868056
2228	1	1.567422244173537
2228	2	1.6933383398075712
2228	3	-0.34423175894869296
2228	4	-0.16349046227486674
2228	5	3.130972851587021
2228	6	0.12642690102323684
2228	7	0.34812254718102764
2228	8	0.3486012721422469
2228	9	0.24962249552479676
2228	10-11	0.17291206644230783
2228	12-13	0.13893046986736124
2228	14-15	0.1662249381464349
2228	16-17	0.110290170053851
2228	18-19	0.17984944954820037
2228	20-21	0.0903610164536417
2228	22-23	0.21192646270374382
2228	24-25	0.1975102662457715
2228	26-27	0.029321694019635913
2228	28-29	0.08988754475879546
2228	30-31	0.18210988673647677
2228	32-33	0.19078154748996923
2228	34-35	0.27008512523038064
2228	36-37	0.20028419882812187
2228	38-39	0.21394626981532383
2228	40-41	0.25813348623712784
2228	42-43	0.4503377904550163
2228	44-45	0.37110320422717535
2228	46-47	0.28107305640186553
2228	48-49	0.2922330203428416
2228	50-51	0.22973133793978917
2228	52-53	0.1346045344121194
2228	54-55	0.1347996569742591
2228	56-57	0.4216721769248011
2228	58-59	0.4415033969972413
2228	60-61	0.27779939624549144
2228	62-63	0.4362009285667128
2228	64-65	0.355090594114543
2228	66-67	0.3979781970013505
2228	68-69	0.3603066917748805
2228	70-71	0.5360051914706929
2228	72-73	0.5243147492036186
2228	74-75	0.2604099045407722
2228	76-77	0.4181449421694765
2228	78-79	0.5592492668642208
2228	80-81	0.5528179334988366
2228	82-83	0.516287592660845
2228	84-85	0.38045508068648815
2228	86-87	0.45010722600177644
2228	88-89	0.3545068574044734
2228	90-91	0.48263177839409366
2228	92-93	0.48344675698299255
2228	94-95	0.6517421481401726
2228	96-97	0.4981678626649355
2228	98-99	0.6025228630080761
2228	100	0.5561702819410002
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	25.0
13	29.0
14	154.0
15	502.0
16	1934.0
17	4658.0
18	7626.0
19	9774.0
20	11458.0
21	13207.0
22	15382.0
23	17550.0
24	20027.0
25	22911.0
26	25642.0
27	29741.0
28	33254.0
29	37976.0
30	43901.0
31	50735.0
32	60082.0
33	71697.0
34	88475.0
35	114489.0
36	160490.0
37	265218.0
38	654813.0
39	1273086.0
40	1627.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.170275005939654	18.221742100261345	18.662315870753147	35.94566702304585
2	19.75680807302345	49.24946110131295	15.141431058019917	15.852299767643682
3	40.82420895627577	16.391406712349205	22.970311181134104	19.814073150240922
4	17.244175213068626	16.38323931495296	21.591338343329063	44.781247128649355
5	17.92147640198481	34.80253176146062	26.35372141863741	20.92227041791716
6	17.732868801628737	28.612500794509927	29.958639377459896	23.69599102640144
7	20.42590342777106	26.317824389758744	35.73401026128097	17.522261921189227
8	21.432370491588404	28.191418765846976	33.0733817602915	17.302828982273123
9	21.123557244069826	28.40515428641811	27.255560169842347	23.215728299669713
10-11	19.848850455283003	30.138881982095615	28.56790943937074	21.444358123250638
12-13	24.1947785960178	26.6687096137842	27.32455491800822	21.811956872189782
14-15	21.743340854145103	27.43110981428063	28.498041965273412	22.327507366300857
16-17	20.79956185864936	29.498646945475706	28.383072673699626	21.318718522175306
18-19	21.4381337760414	29.37776617070585	29.74124828789285	19.442851765359894
20-21	22.74839179663971	28.79292453094274	29.827417623728657	18.631266048688886
22-23	21.7600708455858	30.172457230666073	28.66698853238126	19.400483391366862
24-25	22.170367299058146	28.34044083527446	26.752870033324953	22.736321832342433
26-27	20.350503200598855	26.51249167205397	29.91982777330071	23.217177354046466
28-29	19.829630051662285	28.027292388383675	30.565584624945373	21.577492935008667
30-31	20.257037903382784	29.795742460806668	28.32609339341347	21.621126242397075
32-33	20.873364832701732	29.82488177856934	28.746423058670565	20.55533033005836
34-35	21.450533070878848	29.008883032660037	29.917901189640713	19.622682706820402
36-37	21.87128576900163	28.01927769249946	28.75790022799553	21.35153631050337
38-39	21.371808471573754	29.366770988497375	28.906050084358743	20.355370455570128
40-41	21.10117818003139	29.510356026116934	29.846804815077487	19.541660978774186
42-43	20.606074378083157	29.652137572086435	29.246599410695158	20.495188639135257
44-45	20.722860775843476	30.11184394474756	28.675649925587766	20.489645353821206
46-47	19.97356411952496	27.997300095326594	28.866552156193997	23.162583628954454
48-49	20.932199733703325	28.124383534395115	30.091458384310954	20.851958347590603
50-51	21.929544341557925	30.49865913070569	28.119114245752375	19.452682281984004
52-53	20.40975953550486	30.17385634045412	28.24855956777977	21.16782455626125
54-55	22.042487971326846	28.428151262026613	28.180495811580926	21.348864955065615
56-57	21.421250027584648	28.61522758240893	29.24674311758899	20.716779272417426
58-59	21.111732539135144	27.94250561096805	29.213594912810137	21.73216693708667
60-61	19.68328574775985	30.15339081584984	29.650947098092367	20.51237633829794
62-63	20.444943184428816	31.03131966698676	27.47108910111688	21.052648047467546
64-65	19.974585545475474	30.133056212478937	28.748961373124832	21.14339686892076
66-67	20.23979873952029	30.171666837369663	29.394759626578686	20.193774796531358
68-69	20.917791522570834	29.850026165311416	29.204949969750988	20.02723234236676
70-71	19.707518253968516	30.798135857410415	29.1293850773087	20.364960811312372
72-73	20.09146826422716	30.710533933724864	28.35730255893123	20.840695243116745
74-75	20.086396573908523	29.062745042505046	30.33264360540537	20.51821477818106
76-77	19.575143843346684	30.49457543200757	28.65289318526193	21.277387539383817
78-79	19.616474826138173	29.236532768553413	28.524849471243353	22.622142934065064
80-81	20.668683599660593	29.55582959556338	28.439644408115573	21.33584239666045
82-83	20.574316235699232	28.140635996552565	29.60549823923427	21.679549528513935
84-85	20.699708838869434	28.9149579626032	28.481904768805023	21.903428429722343
86-87	21.51455492788814	28.115590408972547	29.52367606652872	20.846178596610596
88-89	20.807597523829536	27.351428290086194	30.34234238981341	21.49863179627086
90-91	20.316071692623954	27.12188490358684	30.105092668674043	22.456950735115168
92-93	20.201036534942133	27.38607386291221	30.940818313939605	21.47207128820605
94-95	20.298633640521885	27.62019494391995	29.707705972376413	22.373465443181757
96-97	20.199291083079228	27.94605104689239	30.26793015426172	21.586727715766667
98-99	21.278227332261253	28.31985767651376	29.325237949548537	21.07667704167645
100	20.549237715065193	29.43157878096983	28.456398118468755	21.562785385496216
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	5.5
2	8.0
3	15.5
4	28.5
5	37.0
6	46.0
7	47.0
8	51.5
9	56.5
10	64.5
11	81.5
12	96.0
13	111.0
14	141.5
15	185.0
16	246.5
17	340.0
18	475.5
19	747.0
20	1265.5
21	2014.0
22	3065.5
23	4745.0
24	7297.0
25	10905.0
26	15674.5
27	21367.5
28	27682.5
29	34988.0
30	43493.5
31	53072.5
32	63776.0
33	75483.0
34	88824.5
35	103733.5
36	118124.5
37	128842.0
38	136521.5
39	142816.5
40	145919.0
41	145889.0
42	144993.0
43	143445.5
44	141241.0
45	137138.5
46	128416.0
47	117888.5
48	109498.0
49	104676.5
50	101031.5
51	93759.0
52	83461.0
53	71452.0
54	59260.0
55	47973.0
56	37435.5
57	28396.0
58	20523.5
59	14590.0
60	11445.5
61	10004.5
62	8427.5
63	6467.0
64	5005.5
65	4555.5
66	4871.0
67	4607.0
68	3317.5
69	2739.5
70	3240.5
71	3153.5
72	2008.5
73	936.5
74	489.5
75	348.5
76	254.0
77	186.0
78	118.0
79	86.5
80	75.5
81	51.5
82	41.0
83	33.0
84	27.0
85	26.5
86	26.5
87	20.5
88	15.5
89	18.0
90	16.5
91	16.0
92	15.5
93	12.5
94	15.5
95	19.5
96	18.0
97	20.0
98	41.5
99	94.5
100	99.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.286256410830628
2	0.005862083615048167
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	3.9519664820549433E-4
30-31	0.00974818398906886
32-33	0.0
34-35	0.0
36-37	0.0
38-39	8.562594044452378E-4
40-41	0.0013996547957277924
42-43	1.3173221606849813E-4
44-45	0.0
46-47	0.0019595167140189092
48-49	0.0
50-51	0.0
52-53	0.0015807865928219773
54-55	0.01949636797813772
56-57	0.011658301122062084
58-59	0.014655209037620414
60-61	1.1526568905993585E-4
62-63	0.012102897351293265
64-65	6.586610803424906E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.4819874307706038E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3036463.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.40304144849877
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.80991634372258	62.98708846070481
2	8.70879762404415	12.785044659286008
3	2.467841348523142	5.434411823818898
4	1.0523377073446476	3.0897915340014936
5	0.5572835933236356	2.0453155349651584
6	0.35182747991982727	1.5495124254765573
7	0.23417359206249905	1.203233771901522
8	0.15912640630333522	0.9344289757947498
9	0.11464315827623013	0.7573640848863235
>10	0.507807042853281	6.3066156232799475
>50	0.025366210176167286	1.2722510164301941
>100	0.010285324293951096	1.327296890219316
>500	5.027585171651091E-4	0.23675596195907506
>1k	9.141063945228586E-5	0.07088923727594987
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.293305401712453E-5
2	0.0	0.0	0.0	0.0	3.293305401712453E-5
3	0.0	0.0	0.0	0.0	3.293305401712453E-5
4	0.0	0.0	0.0	0.0	3.293305401712453E-5
5	0.0	0.0	0.0	0.0	3.293305401712453E-5
6	0.0	0.0	0.0	0.0	3.293305401712453E-5
7	0.0	0.0	0.0	0.0	3.293305401712453E-5
8	0.0	0.0	0.0	0.0	3.293305401712453E-5
9	0.0	0.0	0.0	0.0	3.293305401712453E-5
10-11	0.0	0.0	0.0	0.0	3.293305401712453E-5
12-13	3.293305401712453E-5	0.0	0.0	0.0	3.293305401712453E-5
14-15	3.293305401712453E-5	0.0	0.0	3.293305401712453E-5	3.293305401712453E-5
16-17	3.293305401712453E-5	0.0	0.0	3.293305401712453E-5	3.293305401712453E-5
18-19	3.293305401712453E-5	0.0	0.0	3.293305401712453E-5	3.293305401712453E-5
20-21	3.293305401712453E-5	0.0	0.0	3.293305401712453E-5	3.293305401712453E-5
22-23	3.293305401712453E-5	0.0	0.0	3.293305401712453E-5	3.293305401712453E-5
24-25	3.293305401712453E-5	0.0	0.0	3.293305401712453E-5	3.293305401712453E-5
26-27	3.293305401712453E-5	0.0	0.0	3.293305401712453E-5	3.293305401712453E-5
28-29	3.293305401712453E-5	0.0	0.0	3.293305401712453E-5	3.293305401712453E-5
30-31	3.293305401712453E-5	0.0	0.0	3.293305401712453E-5	3.293305401712453E-5
32-33	3.293305401712453E-5	0.0	0.0	3.293305401712453E-5	3.293305401712453E-5
34-35	3.293305401712453E-5	0.0	0.0	3.293305401712453E-5	3.293305401712453E-5
36-37	3.293305401712453E-5	0.0	0.0	3.293305401712453E-5	3.293305401712453E-5
38-39	3.293305401712453E-5	0.0	0.0	4.93995810256868E-5	3.293305401712453E-5
40-41	3.293305401712453E-5	0.0	0.0	6.586610803424906E-5	3.293305401712453E-5
42-43	3.293305401712453E-5	0.0	0.0	9.879916205137358E-5	3.293305401712453E-5
44-45	3.293305401712453E-5	0.0	0.0	9.879916205137358E-5	3.293305401712453E-5
46-47	3.293305401712453E-5	0.0	0.0	9.879916205137358E-5	3.293305401712453E-5
48-49	3.293305401712453E-5	0.0	0.0	9.879916205137358E-5	3.293305401712453E-5
50-51	3.293305401712453E-5	0.0	0.0	9.879916205137358E-5	3.293305401712453E-5
52-53	1.1526568905993585E-4	0.0	0.0	9.879916205137358E-5	3.293305401712453E-5
54-55	1.975983241027472E-4	0.0	0.0	9.879916205137358E-5	3.293305401712453E-5
56-57	2.799309591455585E-4	0.0	0.0	9.879916205137358E-5	3.293305401712453E-5
58-59	3.622635941883698E-4	0.0	0.0	9.879916205137358E-5	3.293305401712453E-5
60-61	4.281297022226189E-4	0.0	0.0	9.879916205137358E-5	3.293305401712453E-5
62-63	4.7752928324830564E-4	0.0	0.0	9.879916205137358E-5	3.293305401712453E-5
64-65	4.939958102568679E-4	0.0	0.0	9.879916205137358E-5	3.293305401712453E-5
66-67	5.59861918291117E-4	0.0	0.0	9.879916205137358E-5	3.293305401712453E-5
68-69	6.915941343596151E-4	0.0	0.0	2.799309591455585E-4	3.293305401712453E-5
70-71	7.574602423938642E-4	0.0	0.0	4.610627562397434E-4	3.293305401712453E-5
72-73	8.068598234195509E-4	0.0	0.0	4.939958102568679E-4	3.293305401712453E-5
74-75	8.397928774366755E-4	0.0	0.0	4.939958102568679E-4	3.293305401712453E-5
76-77	8.891924584623623E-4	0.0	0.0	4.939958102568679E-4	3.293305401712453E-5
78-79	0.0010209246745308603	0.0	0.0	4.939958102568679E-4	3.293305401712453E-5
80-81	0.0013831882687192303	0.0	0.0	4.939958102568679E-4	3.293305401712453E-5
82-83	0.0015313870117962904	0.0	0.0	4.939958102568679E-4	3.293305401712453E-5
84-85	0.0016301861738476642	0.0	0.0	5.269288642739925E-4	6.586610803424906E-5
86-87	0.0017454518629076	9.879916205137358E-5	0.0	5.269288642739925E-4	6.586610803424906E-5
88	0.0017454518629076	9.879916205137358E-5	0.0	5.269288642739925E-4	6.586610803424906E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	10905	0.0	80.05894	8
CACCGGC	10770	0.0	77.92456	9
CTTCACC	12180	0.0	74.37596	6
CCTTCAC	13930	0.0	70.322426	5
CGTAGTG	2520	0.0	68.17154	4
TTCACCG	13630	0.0	66.63581	7
CGGGCCC	720	0.0	65.84328	7
GTGTGGC	4685	0.0	65.722916	7
CAGCTGC	105	0.0	65.5322	1
CGCTGCA	65	2.564775E-10	65.14444	1
ATGCAGC	45	2.2231507E-7	62.589893	2
CAGCAGC	85	0.0	62.27042	1
CGCTGCT	85	0.0	62.27042	1
ACCTTCA	5745	0.0	60.54116	4
GGGCCCG	795	0.0	59.631645	8
TGTGGCA	5205	0.0	59.247116	8
CAGCGTA	1210	0.0	59.05397	1
GCGTAGT	1765	0.0	58.77195	3
GCCTTCA	4160	0.0	57.76958	4
TGTGTGG	5115	0.0	56.71075	6
>>END_MODULE