FastQCFastQC Report
Tue 7 Mar 2017
1SN_19A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_19A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3036463
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG109050.080.058948
CACCGGC107700.077.924569
CTTCACC121800.074.375966
CCTTCAC139300.070.3224265
CGTAGTG25200.068.171544
TTCACCG136300.066.635817
CGGGCCC7200.065.843287
GTGTGGC46850.065.7229167
CAGCTGC1050.065.53221
CGCTGCA652.564775E-1065.144441
ATGCAGC452.2231507E-762.5898932
CAGCAGC850.062.270421
CGCTGCT850.062.270421
ACCTTCA57450.060.541164
GGGCCCG7950.059.6316458
TGTGGCA52050.059.2471168
CAGCGTA12100.059.053971
GCGTAGT17650.058.771953
GCCTTCA41600.057.769584
TGTGTGG51150.056.710756
Produced by FastQC (version 0.11.5)