FastQCFastQC Report
Tue 7 Mar 2017
1SN_1A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_1A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3040537
Sequences flagged as poor quality0
Sequence length95
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA181820.5979864740998054No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA79140.26028296975172477No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG69870.2297949342501012No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT63630.20927224368590155No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG62620.20595046204009357No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA61020.20068823369029878No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG56910.18717088461676343No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG55970.18407932546125896No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT54360.17878420818427798No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC51680.16996997569837172No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA50100.16477352520294933No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT49250.16197796639212086No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA45420.1493815072797996No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA44970.1479015055564198No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC40240.13234504299733896No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA38280.12589881326884034No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA37700.12399125549203974No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTTAAAAAAAAGCCATGAGTGCTC32710.10757968082611724No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC32120.1056392341221304No Hit
CAGCCTTATTCAGGACACATCAGTACAAGTGACAGCCCTTCAACCGCTGA31650.10409345454437818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGCA1050.088.999892
GCTGCCG158.5780624E-488.9998863
TGCTGCC301.9390427E-888.9998862
GCAGCTT301.9390427E-888.9998863
GCTGCAT550.088.999883
CAGCAGC3100.087.56441
CAGCTGC4750.086.189361
GCTGCCC1300.085.576813
AGCTGCT1200.085.291562
AGCAGCG1100.084.954432
AGCTGCC2050.084.6584242
CAGCCCT191350.082.441751
CTGCTGC1350.082.4072951
CAGCGTA66050.082.262571
GCTGCAA600.081.583233
AGCTGCG600.081.583232
AGCTGCA1050.080.523712
GCTGCTA501.8189894E-1280.09993
AGCAGCT501.8189894E-1280.09992
AGCCCTT165200.080.0298542
Produced by FastQC (version 0.11.5)