FastQCFastQC Report
Tue 7 Mar 2017
1SN_1A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_1A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3040537
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG120800.081.210288
CACCGGC118750.079.133829
CTTCACC135850.075.2195056
GTGTGGC51600.072.500397
CCTTCAC148950.072.354095
CGGGGCA14250.066.53776694
TTCACCG155050.065.329817
CAGCGTA12950.064.976351
CGTAGTG23200.064.3384864
ACCTTCA56200.063.893524
GGCAGCC250.003273480863.5051881
TGTGGCA60350.062.999848
CAGCTGC1100.062.5429841
GCCTTCA45250.060.5795364
CGGGCCC7600.060.5263067
TGTGTGG58800.059.7112246
TAGCTGC457.995546E-658.8010981
GGGCCCG7800.058.3725748
GCGTAGT15950.057.974533
GTGGCAC60800.057.052229
Produced by FastQC (version 0.11.5)