##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1SN_1A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3040537
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.844948770562569	12.0	12.0	12.0	2.0	32.0
2	14.60623370148102	12.0	12.0	12.0	12.0	22.0
3	13.735260909503815	12.0	12.0	12.0	12.0	22.0
4	13.747521901558837	12.0	12.0	12.0	12.0	22.0
5	22.440063712429744	22.0	22.0	22.0	22.0	27.0
6	26.798098822675072	27.0	22.0	32.0	22.0	32.0
7	34.950839933866945	37.0	37.0	37.0	27.0	41.0
8	37.55678256834237	41.0	37.0	41.0	27.0	41.0
9	38.524873073407754	41.0	41.0	41.0	32.0	41.0
10-11	38.8865261300882	41.0	41.0	41.0	37.0	41.0
12-13	39.064491568430185	41.0	41.0	41.0	37.0	41.0
14-15	39.01846335038844	41.0	41.0	41.0	37.0	41.0
16-17	38.9247687168418	41.0	41.0	41.0	37.0	41.0
18-19	38.85876738220913	41.0	41.0	41.0	37.0	41.0
20-21	38.8745576850405	41.0	41.0	41.0	37.0	41.0
22-23	38.8596285787675	41.0	41.0	41.0	37.0	41.0
24-25	38.88249032983318	41.0	41.0	41.0	37.0	41.0
26-27	38.839815795696616	41.0	41.0	41.0	37.0	41.0
28-29	38.79142993490952	41.0	41.0	41.0	34.5	41.0
30-31	38.67688289930364	41.0	41.0	41.0	32.0	41.0
32-33	38.71981462485081	41.0	41.0	41.0	32.0	41.0
34-35	38.696693051260354	41.0	41.0	41.0	32.0	41.0
36-37	38.6232418812861	41.0	41.0	41.0	32.0	41.0
38-39	38.52217535915531	41.0	41.0	41.0	32.0	41.0
40-41	38.570116068312934	41.0	41.0	41.0	32.0	41.0
42-43	38.310022045447894	41.0	41.0	41.0	32.0	41.0
44-45	38.430472314594425	41.0	41.0	41.0	32.0	41.0
46-47	38.426970959406184	41.0	41.0	41.0	32.0	41.0
48-49	38.385995631692694	41.0	41.0	41.0	32.0	41.0
50-51	38.25581944899865	41.0	41.0	41.0	32.0	41.0
52-53	38.23384602785626	41.0	41.0	41.0	32.0	41.0
54-55	38.10478001089939	41.0	37.0	41.0	32.0	41.0
56-57	37.878642654241666	41.0	37.0	41.0	29.5	41.0
58-59	37.875527250613956	41.0	37.0	41.0	29.5	41.0
60-61	37.881429333042156	41.0	37.0	41.0	29.5	41.0
62-63	37.891031090889534	41.0	37.0	41.0	29.5	41.0
64-65	37.8790373213679	41.0	37.0	41.0	29.5	41.0
66-67	37.74303453633355	41.0	37.0	41.0	27.0	41.0
68-69	37.76017443629201	41.0	37.0	41.0	27.0	41.0
70-71	37.7290287866913	41.0	37.0	41.0	27.0	41.0
72-73	37.58892985022054	41.0	37.0	41.0	27.0	41.0
74-75	37.49863214951832	41.0	37.0	41.0	27.0	41.0
76-77	36.45352284810216	39.0	34.5	41.0	24.5	41.0
78-79	36.94928708316985	41.0	37.0	41.0	27.0	41.0
80-81	37.61883262726288	41.0	37.0	41.0	27.0	41.0
82-83	37.80269192580127	41.0	37.0	41.0	27.0	41.0
84-85	37.731348936059646	41.0	37.0	41.0	27.0	41.0
86-87	37.597474886837425	41.0	37.0	41.0	27.0	41.0
88-89	37.49993767548298	41.0	37.0	41.0	27.0	41.0
90-91	37.35299175770596	41.0	37.0	41.0	27.0	41.0
92-93	37.21945777999083	41.0	37.0	41.0	27.0	41.0
94-95	37.07218379516513	41.0	37.0	41.0	27.0	41.0
96-97	37.023001693450865	41.0	37.0	41.0	27.0	41.0
98-99	36.890382027911514	41.0	37.0	41.0	27.0	41.0
100	36.759854262585854	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.3682909681931026
1101	2	-0.5314691197345969
1101	3	-0.06735930617113617
1101	4	0.09896157224763336
1101	5	-1.0409759847015216
1101	6	-0.8323648878582475
1101	7	-0.3788686351074517
1101	8	-0.10364827736471227
1101	9	-0.17499146267091703
1101	10-11	-0.20471033829805663
1101	12-13	-0.2107997477859911
1101	14-15	-0.11052724700639516
1101	16-17	-0.1786809139828378
1101	18-19	-0.14719553213293324
1101	20-21	-0.0710901722098427
1101	22-23	-0.03536192103131697
1101	24-25	-0.1097684399421297
1101	26-27	-0.07453588200417727
1101	28-29	-0.16757865299933172
1101	30-31	-0.12780163896589158
1101	32-33	-0.18903687984967377
1101	34-35	-0.12151420664874735
1101	36-37	-0.09586165116660794
1101	38-39	-0.12460762121647662
1101	40-41	-0.06721558435356911
1101	42-43	0.07405489480806438
1101	44-45	-0.15580257663668107
1101	46-47	-0.0099623792258825
1101	48-49	-0.015776168669141555
1101	50-51	-0.022729883342805124
1101	52-53	-0.08641095536606258
1101	54-55	-0.13818014526577116
1101	56-57	-0.025537006034006993
1101	58-59	-0.1418591456695637
1101	60-61	-0.16010090625263018
1101	62-63	-0.1273085285931046
1101	64-65	-0.05066758126902471
1101	66-67	-0.052209109536299536
1101	68-69	0.03818022829632639
1101	70-71	-0.051290962291972164
1101	72-73	-0.03082984927966237
1101	74-75	0.05849725796425531
1101	76-77	-0.3782409515079266
1101	78-79	-0.1468321290486685
1101	80-81	-0.006263055176319199
1101	82-83	-0.1612034595999745
1101	84-85	-0.134350535180765
1101	86-87	-0.2066635789791107
1101	88-89	-0.3618795834411088
1101	90-91	-0.15311410495331046
1101	92-93	0.1565811487211448
1101	94-95	-0.07153306897051692
1101	96-97	-0.2906926643399643
1101	98-99	-0.3808135683056193
1101	100	-0.38336587335526673
1102	1	1.7625514738353356
1102	2	0.45148440450441996
1102	3	-0.12064390917654322
1102	4	0.06652845944146613
1102	5	-0.7517192828274979
1102	6	-0.16415982057987577
1102	7	0.0993044927033111
1102	8	-0.1274432222716939
1102	9	-0.11005254036000878
1102	10-11	0.01743256374861346
1102	12-13	-0.2013577621054452
1102	14-15	-0.04888979333495769
1102	16-17	-0.16544561457075702
1102	18-19	0.12045314923204131
1102	20-21	-0.042071104073507115
1102	22-23	-0.03817514479619177
1102	24-25	0.020921461420222442
1102	26-27	0.04582035674341256
1102	28-29	-0.09196487823503219
1102	30-31	-0.13965777815361946
1102	32-33	-0.08257443413333476
1102	34-35	-0.09576482830937039
1102	36-37	-0.11524979144105174
1102	38-39	-0.13470553882301317
1102	40-41	-0.05883614746635146
1102	42-43	-0.04558490066634846
1102	44-45	-0.0037864410159400563
1102	46-47	-0.14868824288343774
1102	48-49	-0.16688324938296972
1102	50-51	-0.2129934829565343
1102	52-53	-0.07488208979180655
1102	54-55	-0.26418921005944895
1102	56-57	-0.176365693009771
1102	58-59	0.013351629061233439
1102	60-61	-0.02621574266566995
1102	62-63	-0.07168302265870352
1102	64-65	-0.25532838124288304
1102	66-67	-0.2696132253602812
1102	68-69	-0.374715141353235
1102	70-71	-0.24658351180811167
1102	72-73	-0.308066394366989
1102	74-75	-0.5380994841712763
1102	76-77	-0.23148715680950005
1102	78-79	-0.44360539261532494
1102	80-81	-0.4687210953342529
1102	82-83	-0.4041241166230378
1102	84-85	-0.28561069437130016
1102	86-87	-0.2858473847579077
1102	88-89	-0.08212046291330921
1102	90-91	-0.5120766247510531
1102	92-93	-0.32631823378617497
1102	94-95	-0.21701626384778905
1102	96-97	-0.390229814845398
1102	98-99	-0.4006677982703408
1102	100	-0.6545821625114385
1103	1	1.8822251486281285
1103	2	0.62721668754339
1103	3	0.36744000058120996
1103	4	0.9204224713127509
1103	5	2.7457453031639325
1103	6	-0.03816147908912271
1103	7	-0.6183876475843775
1103	8	-0.35708957291127064
1103	9	-0.38627754003997694
1103	10-11	-0.40347795213880033
1103	12-13	-0.42360312190664473
1103	14-15	-0.4059587978453649
1103	16-17	-0.5473335130005736
1103	18-19	-0.6560255340879948
1103	20-21	-0.4323310604629711
1103	22-23	-0.25456273812289254
1103	24-25	-0.35159401065040186
1103	26-27	-0.27423281227518004
1103	28-29	-0.4333557075038783
1103	30-31	-0.3111535372914247
1103	32-33	-0.4953913253246043
1103	34-35	-0.5307012687161148
1103	36-37	-0.3844517298770711
1103	38-39	-0.14534389419630855
1103	40-41	-0.38418637597364835
1103	42-43	-0.3103139847453704
1103	44-45	-0.5830286189259013
1103	46-47	-0.6212616063145902
1103	48-49	-0.5023723602598622
1103	50-51	-0.36976385623490415
1103	52-53	-0.43033234091514316
1103	54-55	-0.36935896545066527
1103	56-57	-0.5147522878171884
1103	58-59	-0.6208625917310115
1103	60-61	-0.41828932016684206
1103	62-63	-0.43194543978657407
1103	64-65	-0.4318771884959389
1103	66-67	-0.6060486537350087
1103	68-69	-0.38190092425920596
1103	70-71	-0.630040806617373
1103	72-73	-0.4097374206655999
1103	74-75	-0.5618916954363371
1103	76-77	-0.5625627982016752
1103	78-79	-0.6954289704720935
1103	80-81	-0.3905081603845133
1103	82-83	-0.3532074560015417
1103	84-85	-0.5437450706025615
1103	86-87	-0.29286639218421584
1103	88-89	-0.4059686453094997
1103	90-91	-0.6814298345922936
1103	92-93	-0.4924166547886486
1103	94-95	-0.3601748420814985
1103	96-97	-0.3447570757021623
1103	98-99	-0.454580016761156
1103	100	-0.6004706668803195
1104	1	1.9812127151977066
1104	2	-0.6365420915362563
1104	3	-0.06483871755763637
1104	4	0.05832555894678748
1104	5	-1.0957119298592701
1104	6	-0.5273173295694633
1104	7	-0.07709798043404703
1104	8	-1.132660289897558E-4
1104	9	0.00788118614658373
1104	10-11	-0.1835450069250868
1104	12-13	-0.19209057152847464
1104	14-15	-0.30047519221452745
1104	16-17	-0.11345691172274996
1104	18-19	-0.10744380111420782
1104	20-21	0.026440412246969913
1104	22-23	-0.13476017721585976
1104	24-25	-0.24869107586600592
1104	26-27	-0.06000194105349976
1104	28-29	-0.08150754194851118
1104	30-31	-0.20473122152679224
1104	32-33	-0.07810772666086763
1104	34-35	-0.16612364206596197
1104	36-37	-0.20607227742006984
1104	38-39	-0.22886648857385694
1104	40-41	-0.19027505294261715
1104	42-43	-0.114879070614883
1104	44-45	-0.17543583030133192
1104	46-47	-0.3930555137986005
1104	48-49	-0.3115626054662499
1104	50-51	-0.1466250495570094
1104	52-53	-0.22141878912292867
1104	54-55	-0.1608920190098928
1104	56-57	-0.3090057096943113
1104	58-59	-0.14305486806043177
1104	60-61	-0.22247128660958282
1104	62-63	-0.238969912082041
1104	64-65	-0.32156289406770355
1104	66-67	-0.8183049918412806
1104	68-69	-0.5990040181494152
1104	70-71	-0.356016076254825
1104	72-73	-0.018775456057049666
1104	74-75	-0.14451802211798537
1104	76-77	-0.3904626226734038
1104	78-79	-0.29354685807570036
1104	80-81	-0.218681265813089
1104	82-83	-0.2623031477030864
1104	84-85	-0.26904097686235673
1104	86-87	-0.345758129843027
1104	88-89	-0.3632099863767877
1104	90-91	-0.24237027939188494
1104	92-93	-0.392846126760638
1104	94-95	-0.2462882735845895
1104	96-97	-0.36677609872985073
1104	98-99	-0.37208279471469297
1104	100	-0.3183980824913135
1105	1	1.5545449091411339
1105	2	0.48453088349748263
1105	3	-0.007599511591307007
1105	4	-0.2210176185502064
1105	5	-1.1672822664760574
1105	6	0.3913885766948013
1105	7	0.1216011610466694
1105	8	0.012248151201426083
1105	9	0.13214775549724322
1105	10-11	-0.08262306435337052
1105	12-13	-0.00976995353880028
1105	14-15	-0.087645070158068
1105	16-17	-0.08957767989722498
1105	18-19	-0.05219570653053296
1105	20-21	-0.027102519290423288
1105	22-23	-0.03706650604516426
1105	24-25	-0.1715338068955461
1105	26-27	-0.11265448341355011
1105	28-29	-0.1871151567059215
1105	30-31	-0.09658110222799365
1105	32-33	-0.2727415992641369
1105	34-35	-0.17893586138971074
1105	36-37	-0.22822976449837995
1105	38-39	-0.05514000792773288
1105	40-41	-0.14032305731083028
1105	42-43	0.04790582674439037
1105	44-45	-0.09843680992895543
1105	46-47	-0.13677569339837703
1105	48-49	-0.22768844034801106
1105	50-51	-0.1513305372193301
1105	52-53	-0.25932455744945315
1105	54-55	-0.3013123988423061
1105	56-57	-0.2798180630477418
1105	58-59	-0.1856478910586361
1105	60-61	-0.2174934049473407
1105	62-63	-0.3844118639505254
1105	64-65	-0.30045012295173734
1105	66-67	-0.44976526724612853
1105	68-69	-0.41562595550581705
1105	70-71	-0.4112703631749426
1105	72-73	-0.26531287352523947
1105	74-75	-0.4775495134514003
1105	76-77	-0.4212721110391797
1105	78-79	-0.1750641373777313
1105	80-81	-0.2953976404285328
1105	82-83	-0.06067489347547195
1105	84-85	-0.21063231375615032
1105	86-87	-0.3450132605721947
1105	88-89	-0.30907919569936126
1105	90-91	-0.15708653208709933
1105	92-93	-0.15848986072850835
1105	94-95	0.06360190601656512
1105	96-97	-0.3264693551516089
1105	98-99	-0.35545582092465366
1105	100	-0.41363442814863305
1106	1	1.2715168404821124
1106	2	-0.6442402866939148
1106	3	-0.11726416345025292
1106	4	-0.19969744943892032
1106	5	-1.125025411293997
1106	6	-0.602564024260321
1106	7	-0.26318950929115204
1106	8	-0.10496387975045707
1106	9	-0.18896876856378242
1106	10-11	-0.26196482619754846
1106	12-13	-0.22551724159678344
1106	14-15	-0.10171125513757318
1106	16-17	-0.09717050883200073
1106	18-19	0.0356229918860933
1106	20-21	-0.13593811733797168
1106	22-23	-0.13541035979947225
1106	24-25	-0.2555249793459069
1106	26-27	-0.1373330711949876
1106	28-29	-0.1927468790723168
1106	30-31	-0.0022912120448168594
1106	32-33	-0.2551371576242403
1106	34-35	-0.03863064405898342
1106	36-37	-0.23331209837481026
1106	38-39	-0.18371713705329995
1106	40-41	-0.31736270350545226
1106	42-43	0.0169288512825716
1106	44-45	-0.16147188543859414
1106	46-47	-0.3735721423589524
1106	48-49	-0.3458160286049079
1106	50-51	-0.23748064479072184
1106	52-53	-0.32095636909232894
1106	54-55	-0.33669942927002694
1106	56-57	-0.38483918843181897
1106	58-59	-0.3545186721533895
1106	60-61	-0.2894852780827577
1106	62-63	-0.3926976000722604
1106	64-65	-0.5647648955706401
1106	66-67	-0.5360295404916613
1106	68-69	-0.5144762503548961
1106	70-71	-0.6187150743215852
1106	72-73	-0.4342932212029993
1106	74-75	-0.48650759611735594
1106	76-77	-0.5012250109352223
1106	78-79	-0.2802910720902503
1106	80-81	-0.4052358403442611
1106	82-83	-0.5579903092908012
1106	84-85	-0.5844028177556666
1106	86-87	-0.5424926880935601
1106	88-89	-0.6631189900117747
1106	90-91	-0.7858248323035966
1106	92-93	-0.4557786288687069
1106	94-95	-0.5337300800536013
1106	96-97	-0.5816970000179111
1106	98-99	-0.7225333622775807
1106	100	-0.627250172731344
1107	1	1.358798273678337
1107	2	-0.5876515311752186
1107	3	0.14764717939610605
1107	4	-0.35348339178657007
1107	5	-1.2254599554327932
1107	6	-0.22833274890003707
1107	7	-0.3140291575683065
1107	8	-0.16145211522041336
1107	9	-0.18880929899820842
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2224	54-55	0.3044579126973659
2224	56-57	0.46897881717119105
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2224	64-65	0.41940412358079726
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2224	68-69	0.4448651612970522
2224	70-71	0.514965460259603
2224	72-73	0.6783395884759429
2224	74-75	0.4596050654188133
2224	76-77	0.55456465209231
2224	78-79	0.4414860237689666
2224	80-81	0.4049779035362633
2224	82-83	0.41351851605080014
2224	84-85	0.4609951045405154
2224	86-87	0.5162381514045578
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2224	92-93	0.5479823935795665
2224	94-95	0.636618342741265
2224	96-97	0.6743632826561239
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2224	100	0.6078335972043689
2225	1	1.2901575916662527
2225	2	1.7349456866505104
2225	3	0.7791019026745971
2225	4	1.061905014410378
2225	5	3.1702199474833996
2225	6	-0.16384577317608873
2225	7	0.16495253186466385
2225	8	0.13217389355995834
2225	9	0.16645544551529667
2225	10-11	0.11715605590059397
2225	12-13	0.1376102268414101
2225	14-15	0.16252249355603254
2225	16-17	0.06738517398601118
2225	18-19	-0.00979139415985486
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2225	22-23	0.1775845893636543
2225	24-25	0.21297668247899537
2225	26-27	0.06117917727070221
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2225	30-31	0.12485292137801451
2225	32-33	0.04229373626619548
2225	34-35	0.15268878462817526
2225	36-37	0.21773433992837
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2225	40-41	0.01730180564491235
2225	42-43	0.268091519116048
2225	44-45	0.2715830885553956
2225	46-47	0.11391611446225625
2225	48-49	0.0072204678739993255
2225	50-51	0.16613080178603212
2225	52-53	0.3219603689760291
2225	54-55	0.21432797610890475
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2225	60-61	0.32377037472960524
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2225	64-65	0.32919069472432483
2225	66-67	0.3317169680504648
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2225	70-71	0.2676471919916992
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2225	74-75	0.2793613584738779
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2225	80-81	0.3433966677548881
2225	82-83	0.23227709313498224
2225	84-85	0.2452359156675712
2225	86-87	0.2713202026825101
2225	88-89	0.1607853468533733
2225	90-91	0.3965313759856812
2225	92-93	0.39382306824499125
2225	94-95	0.46519940262339077
2225	96-97	0.4251984049194064
2225	98-99	0.48721576210630957
2225	100	0.580982616865434
2226	1	1.5470909813330636
2226	2	1.2560623977365228
2226	3	0.8573460335471452
2226	4	0.9763275195014156
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2226	84-85	0.3555996737513283
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2226	90-91	0.3817222790634034
2226	92-93	0.378382288813917
2226	94-95	0.5962508296158049
2226	96-97	0.5580829393105304
2226	98-99	0.6695160368988766
2226	100	0.6632581957019354
2227	1	0.9731916700832546
2227	2	1.6671552872368043
2227	3	0.6398567579329342
2227	4	-0.05205614352293075
2227	5	3.2178564443687065
2227	6	0.13589772260991495
2227	7	0.3181221910804979
2227	8	0.3648598591151071
2227	9	0.2038317738406903
2227	10-11	0.23826268836670295
2227	12-13	0.18231524699531576
2227	14-15	0.27556756501077473
2227	16-17	0.2548164675001203
2227	18-19	0.27199680664629966
2227	20-21	0.2753421075892035
2227	22-23	0.24042812179310857
2227	24-25	0.1466520214309881
2227	26-27	0.22937265473496637
2227	28-29	0.23516229662595833
2227	30-31	0.14005995692216544
2227	32-33	0.09506031743146082
2227	34-35	0.13198523021263497
2227	36-37	0.0654807671911044
2227	38-39	0.16556872189038074
2227	40-41	0.1558140812037223
2227	42-43	0.33331687638978025
2227	44-45	0.3578784348360742
2227	46-47	0.28430819947507757
2227	48-49	0.22763087461640197
2227	50-51	0.21706520999416057
2227	52-53	0.43244039829079384
2227	54-55	0.20351815566185394
2227	56-57	0.20894903404884246
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2227	60-61	0.30288360301835837
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2227	66-67	0.3692765136715721
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2227	72-73	0.46454150324969845
2227	74-75	0.3227838575577877
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2227	80-81	0.21679351641741107
2227	82-83	0.2593932529004661
2227	84-85	0.19393507286800826
2227	86-87	0.31602522283642287
2227	88-89	0.2798765890666459
2227	90-91	0.2931968212037148
2227	92-93	0.212621162785112
2227	94-95	0.5291422388271272
2227	96-97	0.25517275449801247
2227	98-99	0.3849805110986111
2227	100	0.350128824267415
2228	1	1.3258584839673322
2228	2	2.003008746651526
2228	3	-0.16859475491366283
2228	4	-0.1295430484806861
2228	5	3.329607598141582
2228	6	0.306500175670287
2228	7	0.43590778380553985
2228	8	0.4348606649226099
2228	9	0.3388802838543583
2228	10-11	0.31986504395252524
2228	12-13	0.20702477752596593
2228	14-15	0.21330626537065456
2228	16-17	0.2876634470694768
2228	18-19	0.21436119065205617
2228	20-21	0.2328682088471581
2228	22-23	0.2984622169698383
2228	24-25	0.36359242801114533
2228	26-27	0.26257420301162426
2228	28-29	0.3154461312560244
2228	30-31	0.36408483264211355
2228	32-33	0.26238463564256165
2228	34-35	0.28593790370359073
2228	36-37	0.3795272790435149
2228	38-39	0.19769570374442935
2228	40-41	0.2603355595936705
2228	42-43	0.4930210847026828
2228	44-45	0.3726572259290748
2228	46-47	0.3994482363491514
2228	48-49	0.3915943990226438
2228	50-51	0.23139906395154242
2228	52-53	0.41868495590583166
2228	54-55	0.4414611101221979
2228	56-57	0.7207307843726696
2228	58-59	0.47333491172219055
2228	60-61	0.4456972280566873
2228	62-63	0.42861411482736855
2228	64-65	0.4362860643881916
2228	66-67	0.4540034850407366
2228	68-69	0.4727750690093657
2228	70-71	0.5100615065652647
2228	72-73	0.6437614038256569
2228	74-75	0.5025090302734512
2228	76-77	0.459262100450033
2228	78-79	0.40506758600316317
2228	80-81	0.3773063478627279
2228	82-83	0.33998372342902883
2228	84-85	0.5325070934886398
2228	86-87	0.4980163771885131
2228	88-89	0.44540305823404935
2228	90-91	0.5362907090398679
2228	92-93	0.5656228359418165
2228	94-95	0.6790428134442976
2228	96-97	0.720731523004261
2228	98-99	0.6783897700010755
2228	100	0.6785719384814399
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	27.0
13	41.0
14	137.0
15	543.0
16	2048.0
17	5055.0
18	8305.0
19	10658.0
20	12474.0
21	14190.0
22	16539.0
23	19031.0
24	21502.0
25	23979.0
26	27276.0
27	30857.0
28	34634.0
29	39689.0
30	45271.0
31	52501.0
32	61408.0
33	73145.0
34	89970.0
35	116297.0
36	162889.0
37	267476.0
38	656848.0
39	1246200.0
40	1547.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.010591975600832	18.510325276512653	20.538758105224375	35.94032464266214
2	19.077601645871262	52.53230318369522	11.05743281579168	17.33266235464184
3	41.535919477381796	14.929665384765913	23.903113167180663	19.63130197067163
4	17.649513885211725	16.229271342529298	22.16628181140371	43.95493296085527
5	16.703398116845808	35.380164753791846	26.62477055862172	21.291666570740627
6	17.31046193484901	27.848501761366496	30.942692031045834	23.898344272738665
7	20.23968134576228	26.491142847464115	36.69450495093465	16.574670855838953
8	20.444645139986786	27.620219717766958	34.84545657559832	17.08967856664793
9	20.797049994787105	28.01498551078313	27.612425042023826	23.57553945240594
10-11	19.328131839869076	30.441399002873503	28.655267145244412	21.57520201201301
12-13	23.983460816296596	26.571506941043637	27.507394253054642	21.937637989605125
14-15	21.70501460761701	27.407395469944944	28.70499520314997	22.182594719288073
16-17	20.659196056486078	30.13900176185983	28.047660659942636	21.154141521711463
18-19	21.416776049757	29.54333395712665	29.752343089395062	19.287546903721285
20-21	22.699279765383547	28.965195950583727	30.065281231571923	18.2702430524608
22-23	21.958851347640234	30.556658248197603	28.649758249940717	18.83473215422144
24-25	22.21420097831403	28.264398690099807	26.464404149661718	23.056996181924443
26-27	19.998029953261547	26.562561152848986	30.13503864613389	23.304370247755575
28-29	19.31044542263712	28.29839093736351	30.88600072092742	21.505162919071953
30-31	20.014975921921273	30.14459346358405	28.333273304654426	21.507157309840245
32-33	20.337032570233482	30.413640748328337	28.901095431497794	20.348231249940387
34-35	21.128718380996514	29.44922229198329	30.14515199124365	19.276907335776542
36-37	21.72897419107217	28.156210564120748	28.750875256574744	21.363939988232342
38-39	21.563482872791532	29.21334327463819	28.93694254883962	20.286231303730652
40-41	20.66408594653173	29.89748379786844	29.99801019241933	19.440420063180504
42-43	20.540352362388916	30.08711132861673	29.367928566506972	20.004607742487384
44-45	20.444431361960074	30.033855861645492	29.09915255101319	20.42256022538124
46-47	19.70658541623088	28.308067552580585	28.900162343001558	23.08518468818698
48-49	20.735094075518266	27.904405670579134	30.4830628547072	20.877437399195404
50-51	21.833330757034037	30.74251357572692	28.27271958867792	19.151436078561122
52-53	20.357447905754945	30.511291074261166	28.322439836355805	20.808821183628083
54-55	22.359270583707378	28.503913696374795	28.298138300934383	20.838677418983444
56-57	21.585131192458366	28.638029202122233	29.688703666547156	20.08813593887224
58-59	21.246251827213676	28.24119088939091	28.82905700643521	21.683500276960203
60-61	19.594863595394425	30.345237337616794	29.640590798023307	20.419308268965473
62-63	20.573942343937798	31.363188720615913	27.36414722090833	20.698721714537957
64-65	19.619711537512817	30.359551973436243	29.060750512903326	20.95998597614761
66-67	20.16601014886515	30.302657063538447	29.568691319987227	19.962641467609178
68-69	20.73482414455078	30.138179538680177	29.35310440228157	19.773891914487475
70-71	19.506718714490237	31.118976680763954	29.283823877163805	20.090480727582
72-73	19.984150825988962	30.735014900328462	28.774423728440073	20.506410545242503
74-75	19.97604370543756	29.44690362261666	30.611582756598587	19.96546991534719
76-77	19.25584197791377	30.62493566103619	28.91670122744765	21.202521133602385
78-79	19.54843831862595	29.64972963657407	28.440831339990268	22.36100070480971
80-81	20.510897351039663	29.682629605176068	28.484878882235265	21.321594161549005
82-83	19.963743246669914	28.549118132750895	30.339377550741858	21.147761069837333
84-85	20.399011753515907	29.147482829513336	28.24912178342181	22.20438363354894
86-87	21.34371329801282	28.996867987464057	29.41662936514175	20.242789349381376
88-89	20.743769932745433	29.485761890087183	28.854771377556006	20.915696799611386
90-91	21.21926159754017	29.992169146436957	27.770341225908453	21.018228030114415
92-93	21.17535487974657	29.776976895857544	28.774308617194922	20.273359607200963
94-95	20.988035994957468	30.73940557210782	28.2808760426201	19.991682390314605
96-97	20.767861071909337	29.949035318432237	28.71616099392969	20.566942615728735
98-99	21.6969239315292	30.333177987967257	27.972147683123076	19.99775039738046
100	21.900111723685654	31.875389117119774	26.459437921656605	19.765061237537974
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	8.0
2	12.0
3	17.0
4	24.0
5	31.5
6	45.0
7	49.5
8	59.0
9	65.5
10	63.0
11	89.5
12	118.5
13	131.5
14	157.5
15	207.0
16	270.5
17	348.0
18	498.0
19	806.0
20	1311.5
21	2052.5
22	3267.5
23	5143.0
24	8006.5
25	12009.0
26	17111.5
27	23150.5
28	29883.5
29	37499.0
30	46277.0
31	56281.0
32	67482.0
33	79641.5
34	92885.5
35	107854.0
36	123188.0
37	134302.5
38	141085.5
39	145823.5
40	148106.0
41	148287.0
42	146264.0
43	142996.5
44	141114.0
45	137113.0
46	126982.0
47	115290.5
48	107110.0
49	102253.0
50	97583.0
51	90051.0
52	78633.0
53	66793.0
54	56624.0
55	46671.5
56	36919.0
57	27516.0
58	19002.0
59	12999.5
60	9926.5
61	8519.0
62	7048.0
63	5186.0
64	3837.0
65	3284.0
66	3089.0
67	2632.5
68	1996.5
69	1876.0
70	2203.0
71	2060.5
72	1247.0
73	571.0
74	285.0
75	180.0
76	138.0
77	106.5
78	78.5
79	65.0
80	47.0
81	34.5
82	26.0
83	25.5
84	25.5
85	14.5
86	15.5
87	22.0
88	17.0
89	11.0
90	14.0
91	12.0
92	8.5
93	10.0
94	11.5
95	15.0
96	16.0
97	15.5
98	35.0
99	104.0
100	120.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.272350903804163
2	0.006643563291615921
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	2.960003446759569E-4
30-31	0.01063956794474134
32-33	0.0
34-35	0.0
36-37	0.0
38-39	7.893342524692185E-4
40-41	0.0013813349418211323
42-43	8.222231796554358E-5
44-45	0.0
46-47	0.0020062245583592634
48-49	3.288892718621743E-5
50-51	0.0
52-53	0.0012497792330762624
54-55	0.018812466350516373
56-57	0.011412457733617449
58-59	0.012793792675438584
60-61	1.808890995241959E-4
62-63	0.012201791986086669
64-65	9.866678155865232E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.4800017233797846E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3040537.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.12432981262218
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.0930216801136	61.37277160409123
2	9.131319808107135	13.171806429288976
3	2.5517465631959007	5.521290321664988
4	1.1186152408387158	3.2271749825470932
5	0.5924266870052773	2.1364188881683854
6	0.373259887826578	1.615267155325588
7	0.2493508979322481	1.2588986481077262
8	0.16749814738893795	0.966455330022637
9	0.11744636908994292	0.7623666593584199
>10	0.5664026964485667	6.853524665803418
>50	0.027687618268744673	1.3565070298803443
>100	0.010481338724255792	1.3572785441987816
>500	6.037403617107466E-4	0.28183255403182
>1k	1.3932469836549791E-4	0.11840718751069655
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	3.288892718621743E-5	0.0	0.0	0.0	0.0
14-15	3.288892718621743E-5	0.0	0.0	0.0	0.0
16-17	3.288892718621743E-5	0.0	0.0	0.0	0.0
18-19	3.288892718621743E-5	0.0	0.0	0.0	0.0
20-21	3.288892718621743E-5	0.0	0.0	0.0	0.0
22-23	3.288892718621743E-5	0.0	1.6444463593108716E-5	0.0	0.0
24-25	3.288892718621743E-5	0.0	3.288892718621743E-5	0.0	0.0
26-27	3.288892718621743E-5	0.0	3.288892718621743E-5	0.0	0.0
28-29	3.288892718621743E-5	0.0	3.288892718621743E-5	0.0	0.0
30-31	3.288892718621743E-5	0.0	3.288892718621743E-5	0.0	0.0
32-33	3.288892718621743E-5	0.0	3.288892718621743E-5	0.0	0.0
34-35	4.9333390779326145E-5	0.0	3.288892718621743E-5	0.0	0.0
36-37	6.577785437243486E-5	0.0	3.288892718621743E-5	0.0	0.0
38-39	6.577785437243486E-5	0.0	3.288892718621743E-5	0.0	0.0
40-41	6.577785437243486E-5	0.0	3.288892718621743E-5	0.0	0.0
42-43	6.577785437243486E-5	0.0	3.288892718621743E-5	0.0	0.0
44-45	6.577785437243486E-5	0.0	3.288892718621743E-5	0.0	0.0
46-47	1.3155570874486973E-4	0.0	3.288892718621743E-5	0.0	0.0
48-49	1.3155570874486973E-4	0.0	3.288892718621743E-5	0.0	0.0
50-51	1.3155570874486973E-4	0.0	3.288892718621743E-5	0.0	0.0
52-53	2.137780267104133E-4	0.0	3.288892718621743E-5	0.0	0.0
54-55	2.6311141748973946E-4	0.0	3.288892718621743E-5	0.0	0.0
56-57	2.6311141748973946E-4	0.0	3.288892718621743E-5	0.0	0.0
58-59	2.795558810828482E-4	0.0	3.288892718621743E-5	0.0	0.0
60-61	3.617781990483918E-4	0.0	3.288892718621743E-5	0.0	0.0
62-63	4.7688944420015277E-4	0.0	3.288892718621743E-5	0.0	0.0
64-65	5.920006893519138E-4	0.0	3.288892718621743E-5	0.0	0.0
66-67	6.577785437243487E-4	0.0	3.288892718621743E-5	0.0	0.0
68-69	7.56445325283001E-4	0.0	3.288892718621743E-5	1.973335631173046E-4	0.0
70-71	8.386676432485446E-4	0.0	3.288892718621743E-5	3.946671262346092E-4	0.0
72-73	8.880010340278707E-4	0.0	3.288892718621743E-5	3.946671262346092E-4	0.0
74-75	9.537788884003056E-4	0.0	3.288892718621743E-5	3.946671262346092E-4	0.0
76-77	0.0010853345971451752	0.0	3.288892718621743E-5	3.946671262346092E-4	0.0
78-79	0.0012662236966693712	0.0	3.288892718621743E-5	3.946671262346092E-4	0.0
80-81	0.0014142238690073496	0.0	3.288892718621743E-5	3.946671262346092E-4	0.0
82-83	0.0014964461869728931	0.0	3.288892718621743E-5	3.946671262346092E-4	0.0
84-85	0.0015293351141591107	0.0	3.288892718621743E-5	3.946671262346092E-4	0.0
86-87	0.0015457795777522195	0.0	3.288892718621743E-5	3.946671262346092E-4	0.0
88	0.0015457795777522195	0.0	3.288892718621743E-5	3.946671262346092E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	12080	0.0	81.21028	8
CACCGGC	11875	0.0	79.13382	9
CTTCACC	13585	0.0	75.219505	6
GTGTGGC	5160	0.0	72.50039	7
CCTTCAC	14895	0.0	72.35409	5
CGGGGCA	1425	0.0	66.537766	94
TTCACCG	15505	0.0	65.32981	7
CAGCGTA	1295	0.0	64.97635	1
CGTAGTG	2320	0.0	64.338486	4
ACCTTCA	5620	0.0	63.89352	4
GGCAGCC	25	0.0032734808	63.505188	1
TGTGGCA	6035	0.0	62.99984	8
CAGCTGC	110	0.0	62.542984	1
GCCTTCA	4525	0.0	60.579536	4
CGGGCCC	760	0.0	60.526306	7
TGTGTGG	5880	0.0	59.711224	6
TAGCTGC	45	7.995546E-6	58.801098	1
GGGCCCG	780	0.0	58.372574	8
GCGTAGT	1595	0.0	57.97453	3
GTGGCAC	6080	0.0	57.05222	9
>>END_MODULE