FastQCFastQC Report
Tue 7 Mar 2017
1SN_20A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_20A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2155177
Sequences flagged as poor quality0
Sequence length94
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA124160.5761011740567017No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG73340.3402968758482482No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT49840.2312571078848744No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA47900.22225552704023846No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA42830.19873077710090634No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG41310.19167799210923278No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG40980.19014679536761947No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC40270.18685240237808773No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG39320.18244441175829176No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA33860.15711006566978025No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG33580.15581086843447198No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT32750.15195967662980814No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC32030.1486188837390154No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT31220.1448604917368736No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA30590.14193729795742993No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC30380.14096290003094875No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA30300.14059170082086064No Hit
CAGCAGGCGCGCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACGAAA24730.11474695581847802No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA24580.11405095729956287No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG24550.11391175759577984No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA24540.11386535769451883No Hit
CAGCACTTTGAACTTTGTTCACCATTTCTTCGTAGTCGTTGATACGTTGT22600.10486377684988286No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTTAAAAAAAAGCCATGAGTGCTC22350.10370377931835761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCAT401.8189894E-1188.000013
GCTGCCT158.9715206E-488.03
GCTGCAG257.2899275E-788.03
AGCTGCA1200.088.02
GCAGCTA158.9715206E-488.03
GCAGCAT356.0572347E-1088.03
AGCAGCT356.0572347E-1088.02
CAGCTGC3350.086.686571
CAGCAGC2450.086.204081
AGCTGCC1200.084.333332
AGCAGCG1000.083.6000062
GCTGCCC900.083.1111153
CAGCCCT131200.082.51
CAGCGTA51100.081.455971
GCAGCGT550.080.03
GCAGCAA550.080.03
AGCCCTT113000.079.316822
GCTGCAA501.8189894E-1279.2000053
AGCTGCG501.8189894E-1279.2000052
AGCAGCA1000.079.2000052
Produced by FastQC (version 0.11.5)