##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1SN_20A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2155177
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.843096877889844	12.0	12.0	12.0	2.0	32.0
2	14.52463254758194	12.0	12.0	12.0	12.0	22.0
3	13.661842623598897	12.0	12.0	12.0	12.0	22.0
4	13.772246548659345	12.0	12.0	12.0	12.0	22.0
5	22.423109099623836	22.0	22.0	22.0	22.0	27.0
6	26.826007330256402	27.0	22.0	32.0	22.0	32.0
7	34.91829209387443	37.0	37.0	37.0	27.0	41.0
8	37.55689161493464	41.0	37.0	41.0	27.0	41.0
9	38.501296645240735	41.0	37.0	41.0	32.0	41.0
10-11	38.86293237167992	41.0	41.0	41.0	34.5	41.0
12-13	39.04603983802723	41.0	41.0	41.0	37.0	41.0
14-15	38.99361050159685	41.0	41.0	41.0	37.0	41.0
16-17	38.91494619699449	41.0	41.0	41.0	37.0	41.0
18-19	38.8448320021975	41.0	41.0	41.0	37.0	41.0
20-21	38.851185076678156	41.0	41.0	41.0	37.0	41.0
22-23	38.83773165730703	41.0	41.0	41.0	34.5	41.0
24-25	38.84925089679409	41.0	41.0	41.0	37.0	41.0
26-27	38.82576001878268	41.0	41.0	41.0	37.0	41.0
28-29	38.76412470994262	41.0	41.0	41.0	34.5	41.0
30-31	38.663578443905074	41.0	41.0	41.0	32.0	41.0
32-33	38.71172135745695	41.0	41.0	41.0	32.0	41.0
34-35	38.67878485154584	41.0	41.0	41.0	32.0	41.0
36-37	38.591263965790276	41.0	41.0	41.0	32.0	41.0
38-39	38.4855870306708	41.0	41.0	41.0	32.0	41.0
40-41	38.54380498678299	41.0	41.0	41.0	32.0	41.0
42-43	38.315648552299876	41.0	41.0	41.0	32.0	41.0
44-45	38.4272031949116	41.0	41.0	41.0	32.0	41.0
46-47	38.41310551291147	41.0	41.0	41.0	32.0	41.0
48-49	38.369812781038405	41.0	41.0	41.0	32.0	41.0
50-51	38.26607512979213	41.0	41.0	41.0	32.0	41.0
52-53	38.2524377348125	41.0	41.0	41.0	32.0	41.0
54-55	38.118671923466145	41.0	39.0	41.0	32.0	41.0
56-57	37.914295438379305	41.0	37.0	41.0	29.5	41.0
58-59	37.8909774000001	41.0	37.0	41.0	29.5	41.0
60-61	37.893621962372464	41.0	37.0	41.0	29.5	41.0
62-63	37.88656128939758	41.0	37.0	41.0	29.5	41.0
64-65	37.86090399999628	41.0	37.0	41.0	29.5	41.0
66-67	37.76663981659047	41.0	37.0	41.0	27.0	41.0
68-69	37.761879650720104	41.0	37.0	41.0	27.0	41.0
70-71	37.73215169798118	41.0	37.0	41.0	27.0	41.0
72-73	37.59197434827858	41.0	37.0	41.0	27.0	41.0
74-75	37.49118448275942	41.0	37.0	41.0	27.0	41.0
76-77	36.44050651060215	39.0	34.5	41.0	24.5	41.0
78-79	36.964743499025836	41.0	37.0	41.0	27.0	41.0
80-81	37.63566008731534	41.0	37.0	41.0	27.0	41.0
82-83	37.820261166484244	41.0	37.0	41.0	27.0	41.0
84-85	37.730517957457785	41.0	37.0	41.0	27.0	41.0
86-87	37.59226527565949	41.0	37.0	41.0	27.0	41.0
88-89	37.49623534401119	41.0	37.0	41.0	27.0	41.0
90-91	37.339203694174536	41.0	37.0	41.0	27.0	41.0
92-93	37.25910679262075	41.0	37.0	41.0	27.0	41.0
94-95	37.110581404682776	41.0	37.0	41.0	27.0	41.0
96-97	37.04783017821738	41.0	37.0	41.0	27.0	41.0
98-99	36.93525079378631	41.0	37.0	41.0	27.0	41.0
100	36.808617111262784	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.280162459751251
1101	2	-0.5755915061502037
1101	3	0.09879553907281569
1101	4	0.050064377140117244
1101	5	-0.9757051969145465
1101	6	-0.7213651577742475
1101	7	-0.0845731876899265
1101	8	0.26991824279959786
1101	9	0.30370435110088323
1101	10-11	-0.13194294609168367
1101	12-13	-0.048227796571460146
1101	14-15	-0.025220977724444538
1101	16-17	-0.004278107978002765
1101	18-19	0.03709748011482361
1101	20-21	0.05557231252186057
1101	22-23	0.15901940287133698
1101	24-25	0.015504760087729608
1101	26-27	0.04771592765856525
1101	28-29	0.15502973025228073
1101	30-31	0.07586631802249855
1101	32-33	-0.08965440368693578
1101	34-35	-0.1165276052099955
1101	36-37	0.14828418850106573
1101	38-39	0.11436132083462525
1101	40-41	0.17435816086162248
1101	42-43	0.1714906930280904
1101	44-45	-0.010006685006615612
1101	46-47	0.04179103960782271
1101	48-49	-0.00899647433116968
1101	50-51	0.21511895862294494
1101	52-53	0.12748267709212513
1101	54-55	0.062227792692418404
1101	56-57	0.24049305261348053
1101	58-59	0.010452029308609667
1101	60-61	-0.02584329894275328
1101	62-63	-0.02877196553539818
1101	64-65	0.08982511972362062
1101	66-67	0.010005883924975478
1101	68-69	0.05089753837151534
1101	70-71	0.0991753942209499
1101	72-73	0.09434307468436742
1101	74-75	-0.1610583056886341
1101	76-77	-0.5416999614499076
1101	78-79	-0.10797096412547802
1101	80-81	0.06246050896613298
1101	82-83	-0.1857220622082565
1101	84-85	0.14794671396681736
1101	86-87	-0.05330162791234727
1101	88-89	-0.435537072592588
1101	90-91	-0.12786576997069687
1101	92-93	0.02568789507186864
1101	94-95	-0.021845341397359164
1101	96-97	-0.16433563017626085
1101	98-99	-0.16563942432415502
1101	100	-0.390551816241107
1102	1	1.8508501680329434
1102	2	0.788279710352592
1102	3	-0.012047502597294368
1102	4	0.013253944465022371
1102	5	-0.7736533640356456
1102	6	0.04719305093433945
1102	7	-0.024383978402973128
1102	8	-0.3583226892007332
1102	9	-0.34362330870797564
1102	10-11	-0.2874262973269168
1102	12-13	-0.39558409941648165
1102	14-15	-0.33351217274496747
1102	16-17	-0.23895367051070338
1102	18-19	-0.21008131853013623
1102	20-21	-0.1575636868171415
1102	22-23	-0.2601290176304687
1102	24-25	-0.127569546558
1102	26-27	-0.08136556728758393
1102	28-29	-0.1908858829565645
1102	30-31	-0.08952917620758427
1102	32-33	-0.2975721375821152
1102	34-35	-0.30352945048251456
1102	36-37	-0.20876296234308
1102	38-39	-0.32245723654784086
1102	40-41	-0.25856480589593644
1102	42-43	-0.16878761262615427
1102	44-45	-0.0954823246551797
1102	46-47	-0.49446002762068986
1102	48-49	-0.36550395181609474
1102	50-51	-0.08469968138366823
1102	52-53	-0.18206605738141945
1102	54-55	-0.2330215945118539
1102	56-57	-0.31676506520854986
1102	58-59	-0.2341962672569835
1102	60-61	-0.14176235482421617
1102	62-63	-0.31399394906564737
1102	64-65	-0.40456112060246596
1102	66-67	-0.10991286900129893
1102	68-69	-0.3317144861953949
1102	70-71	-0.42856345840512233
1102	72-73	-0.3908351180502123
1102	74-75	-0.5319580826034453
1102	76-77	-0.363769696226413
1102	78-79	-0.5167680943135835
1102	80-81	-0.4762519314756304
1102	82-83	-0.3816780235401538
1102	84-85	-0.5604933422175975
1102	86-87	-0.4676900715489083
1102	88-89	-0.35845521960848004
1102	90-91	-0.5265267080301328
1102	92-93	-0.39044515733937857
1102	94-95	-0.18412659397936437
1102	96-97	-0.32626187435813847
1102	98-99	-0.5383665718633353
1102	100	-0.5164613703461498
1103	1	1.8619112431807068
1103	2	0.7717866059337908
1103	3	0.47168455481897453
1103	4	1.079353015672453
1103	5	2.990742920794073
1103	6	0.023928204325837754
1103	7	-0.45147939073058296
1103	8	-0.21680847585989937
1103	9	-0.25467312576398626
1103	10-11	-0.22278655626303134
1103	12-13	-0.5099466099709389
1103	14-15	-0.3166348859450778
1103	16-17	-0.2696755956443937
1103	18-19	-0.3825538769314889
1103	20-21	-0.16665881769246482
1103	22-23	-0.08860636072081007
1103	24-25	-0.17754280859008276
1103	26-27	-0.14515954420849653
1103	28-29	-0.16917183848710238
1103	30-31	-0.12373548073896501
1103	32-33	-0.37792817473381746
1103	34-35	-0.2317168977102071
1103	36-37	-0.18421328601321818
1103	38-39	-0.268315666044046
1103	40-41	-0.47531121737918625
1103	42-43	-0.32027790252201527
1103	44-45	-0.552984435547387
1103	46-47	-0.722062054077206
1103	48-49	-0.44914875902126994
1103	50-51	-0.2787737033369453
1103	52-53	-0.11490168924181887
1103	54-55	-0.30458928378852335
1103	56-57	-0.3258855267903158
1103	58-59	-0.6585855157793645
1103	60-61	-0.311749689470993
1103	62-63	-0.3414988744807914
1103	64-65	-0.4685943319731365
1103	66-67	-0.40777101704519936
1103	68-69	-0.4730682717287493
1103	70-71	-0.538177868762574
1103	72-73	-0.4746108005720302
1103	74-75	-0.5163374802955332
1103	76-77	-0.549173580268068
1103	78-79	-0.5595896535592928
1103	80-81	-0.40458854841906344
1103	82-83	-0.6131148375001914
1103	84-85	-0.4164179130028458
1103	86-87	-0.5051907751796421
1103	88-89	-0.4234721067461038
1103	90-91	-0.7722598912555725
1103	92-93	-0.5617581322901231
1103	94-95	-0.41417444086931
1103	96-97	-0.44525708966914834
1103	98-99	-0.33308934135390444
1103	100	-0.322217069755105
1104	1	1.7464828668011414
1104	2	-0.6393238730740602
1104	3	0.04542730143406182
1104	4	0.1546033285635655
1104	5	-1.0433622106201916
1104	6	-0.4466031416748457
1104	7	-0.17213873764149668
1104	8	-0.12937532077967973
1104	9	0.16412025232225602
1104	10-11	-0.09110490874908805
1104	12-13	-0.06760817556430254
1104	14-15	-0.12187723433511621
1104	16-17	-0.029538776893680563
1104	18-19	-0.15530248314379946
1104	20-21	0.12860372639674011
1104	22-23	0.01956863074578763
1104	24-25	-0.03411209974948548
1104	26-27	-0.3046970342528752
1104	28-29	-0.174004583964404
1104	30-31	-0.03703477531172439
1104	32-33	-0.20502454519689906
1104	34-35	-0.18223885697748443
1104	36-37	-0.2134942053442046
1104	38-39	-0.1290193866038365
1104	40-41	-0.1541555113830526
1104	42-43	-0.23679713110320222
1104	44-45	-0.09194649038866487
1104	46-47	-0.33776349906526093
1104	48-49	-0.4458818913457634
1104	50-51	-0.24202890870729732
1104	52-53	-0.08805989836686479
1104	54-55	-0.1945753459116233
1104	56-57	-0.06199742803050157
1104	58-59	-0.19451821574522654
1104	60-61	-0.026647573189279683
1104	62-63	-0.12026494570619661
1104	64-65	-0.11998385296528369
1104	66-67	-0.6576060380942437
1104	68-69	-0.43342781206325753
1104	70-71	-0.33981485818115686
1104	72-73	-0.22422537560900224
1104	74-75	-0.28064229312975897
1104	76-77	-0.4215552505377218
1104	78-79	-0.2890469296999214
1104	80-81	-0.23786167391683932
1104	82-83	-0.24197477605975592
1104	84-85	-0.14296814624894694
1104	86-87	-0.09087316225439679
1104	88-89	-0.1117642901571898
1104	90-91	-0.20915828697750527
1104	92-93	-0.3054647542038822
1104	94-95	-0.20706612365461297
1104	96-97	-0.19001047458210252
1104	98-99	-0.2526862807099164
1104	100	-0.4227603625074039
1105	1	1.3667056374274331
1105	2	0.3670290916428076
1105	3	-0.008461735672144144
1105	4	-0.15394343770513608
1105	5	-1.0688796743254798
1105	6	0.5919348427178726
1105	7	0.04314228239007889
1105	8	-0.08930217553412234
1105	9	-0.014203520994179542
1105	10-11	0.08816986392260162
1105	12-13	-0.12973577955727222
1105	14-15	-0.023617490899283666
1105	16-17	-0.013556596320526637
1105	18-19	-0.13332528267247312
1105	20-21	-0.05035173059430065
1105	22-23	0.06234637146923916
1105	24-25	-0.132423256066879
1105	26-27	3.5032117965982934E-4
1105	28-29	-0.09199131110373315
1105	30-31	-0.033720728756662766
1105	32-33	0.018549789492624313
1105	34-35	0.030969807065737598
1105	36-37	-0.06238035221274174
1105	38-39	-0.075965183531288
1105	40-41	0.017008531837113594
1105	42-43	0.16862123079901892
1105	44-45	0.09017433266208741
1105	46-47	-0.18647289678420265
1105	48-49	-0.06705705086465485
1105	50-51	-0.054973838563448396
1105	52-53	-0.09570874560668585
1105	54-55	-0.09662936000709266
1105	56-57	-0.10274950122020954
1105	58-59	-0.0900041647436467
1105	60-61	0.08509707431288405
1105	62-63	-0.1900113829324539
1105	64-65	-0.3239204122485049
1105	66-67	-0.2488030411401141
1105	68-69	-0.43549989014414336
1105	70-71	-0.4658900821745817
1105	72-73	-0.1413358605019539
1105	74-75	-0.22089830148922118
1105	76-77	-0.36601850005416026
1105	78-79	-0.30555129840531237
1105	80-81	-0.2576372914272369
1105	82-83	-0.15356907562176758
1105	84-85	-0.27275204540188724
1105	86-87	-0.14796230385380937
1105	88-89	-0.11200551383935675
1105	90-91	-0.21051669868074185
1105	92-93	-0.17855080547225555
1105	94-95	-0.09594037608352579
1105	96-97	0.02757467626607024
1105	98-99	-0.17264309388940546
1105	100	-0.345777214816259
1106	1	1.1431946656405998
1106	2	-0.5065938371525345
1106	3	-0.0049388218044015275
1106	4	-0.2670946543046302
1106	5	-1.1924503565168507
1106	6	-0.6951856230232849
1106	7	-0.27933271387802705
1106	8	0.011274029869667856
1106	9	-0.22177778580982022
1106	10-11	-0.15884461585049792
1106	12-13	-0.11941185061265713
1106	14-15	-0.05154703262192584
1106	16-17	-0.1309666575236932
1106	18-19	-0.03955158510567003
1106	20-21	-0.012625204247086685
1106	22-23	-0.029630042920963717
1106	24-25	-0.4095825335709833
1106	26-27	-0.34392195826503524
1106	28-29	-0.3275981180898526
1106	30-31	-0.19556130197654653
1106	32-33	-0.312435432182248
1106	34-35	-0.3216634217743817
1106	36-37	-0.38850663971833654
1106	38-39	-0.18736837803793804
1106	40-41	-0.2303761524441157
1106	42-43	-0.2292694991606723
1106	44-45	-0.2857728236298982
1106	46-47	-0.5031544910519941
1106	48-49	-0.3886139996629794
1106	50-51	-0.41412893633923886
1106	52-53	-0.350463186568021
1106	54-55	-0.3572936382575804
1106	56-57	-0.2432346482570793
1106	58-59	-0.1869201223492638
1106	60-61	-0.2803440363006331
1106	62-63	-0.40575403108888963
1106	64-65	-0.7167073953803182
1106	66-67	-0.5211705586800406
1106	68-69	-0.35118133513593364
1106	70-71	-0.7760617495876261
1106	72-73	-0.6029301283031216
1106	74-75	-0.7547332022206703
1106	76-77	-0.5283561623918231
1106	78-79	-0.47262250306273046
1106	80-81	-0.5747413368071435
1106	82-83	-0.6598222477370186
1106	84-85	-0.5799396853481511
1106	86-87	-0.6664665582201366
1106	88-89	-0.6535714191983573
1106	90-91	-0.6687686765878951
1106	92-93	-0.6284731819463474
1106	94-95	-0.5205380772329491
1106	96-97	-0.6586337130457665
1106	98-99	-0.7597814727519179
1106	100	-0.8482522110843647
1107	1	1.2408983133442462
1107	2	-0.4327838633425607
1107	3	-0.13921130607688603
1107	4	-0.24812038533035974
1107	5	-1.231832595899089
1107	6	-0.26060101843867756
1107	7	-0.3029002274455408
1107	8	-0.36263935404371495
1107	9	-0.3250523490843875
1107	10-11	-0.2026555346614174
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2225	4	0.8970121470520436
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2226	1	1.4089318106366218
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2226	3	1.0167414022432943
2226	4	0.758578591646538
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2226	62-63	-6.52534541174532E-4
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2226	90-91	0.23888597447385962
2226	92-93	-0.05961180143288658
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2226	100	0.13241177752610156
2227	1	1.1874901550973789
2227	2	1.4999136338710635
2227	3	0.7162732994077192
2227	4	-0.08900034717806449
2227	5	3.206730391050993
2227	6	0.13228912445146435
2227	7	0.35949426559411535
2227	8	0.4042396305772016
2227	9	0.30951114031781657
2227	10-11	0.31795098997736204
2227	12-13	0.2312970820317588
2227	14-15	0.3485460642453546
2227	16-17	0.37635354915135366
2227	18-19	0.21188071019759747
2227	20-21	0.07306301434435625
2227	22-23	0.4127569607563615
2227	24-25	0.32176225067702546
2227	26-27	0.30439345392134953
2227	28-29	0.20819111060259843
2227	30-31	0.3521108108568498
2227	32-33	0.37242422429030597
2227	34-35	0.24642204114979194
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2227	54-55	0.35754972626320836
2227	56-57	0.5320816483495392
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2227	82-83	0.3360759042901691
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2227	86-87	0.4018814378698039
2227	88-89	0.30785854868322104
2227	90-91	0.41989957272551237
2227	92-93	0.47741333297241795
2227	94-95	0.7234436757810627
2227	96-97	0.5350645600073491
2227	98-99	0.6525017279183771
2227	100	0.7214708257354516
2228	1	1.705516907157472
2228	2	1.5408379257154685
2228	3	-0.14170541091998423
2228	4	-0.20931350927516235
2228	5	3.161838307838927
2228	6	0.1124089300746256
2228	7	0.12083597649201039
2228	8	0.14752194303033406
2228	9	0.15994813927180473
2228	10-11	0.0982573425937403
2228	12-13	0.027892791054398458
2228	14-15	0.28587795849433917
2228	16-17	0.09850373785274513
2228	18-19	-0.003329015728738227
2228	20-21	0.05261170649318103
2228	22-23	0.17152383675640692
2228	24-25	0.29927867233720207
2228	26-27	0.158178491620653
2228	28-29	0.035126942084872326
2228	30-31	-0.04080719702110258
2228	32-33	0.1522443175981678
2228	34-35	0.17947329385897604
2228	36-37	0.2235706789361629
2228	38-39	0.16884279334102104
2228	40-41	0.20622067354867823
2228	42-43	0.3109166234415568
2228	44-45	0.15888131417860762
2228	46-47	0.15866824996538043
2228	48-49	0.18007140888819606
2228	50-51	0.09088940102809318
2228	52-53	0.18618204739264854
2228	54-55	0.17591436179606035
2228	56-57	0.3463057442363251
2228	58-59	0.2321691105654793
2228	60-61	0.23613170272544437
2228	62-63	0.3681617175919598
2228	64-65	0.2807332847449189
2228	66-67	0.2952636111769422
2228	68-69	0.27373669984922344
2228	70-71	0.3304749352040943
2228	72-73	0.4030117150883896
2228	74-75	0.3196029314443294
2228	76-77	0.37373838735373255
2228	78-79	0.47017604067541185
2228	80-81	0.29824661483852566
2228	82-83	0.3222905388052979
2228	84-85	0.3234495428732913
2228	86-87	0.3501239653502566
2228	88-89	0.36453597676138116
2228	90-91	0.4080074332083541
2228	92-93	0.3135497615531051
2228	94-95	0.633934161937276
2228	96-97	0.5580399488627918
2228	98-99	0.5850866792303293
2228	100	0.9169964999361611
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	17.0
13	21.0
14	109.0
15	367.0
16	1477.0
17	3603.0
18	5892.0
19	7597.0
20	8973.0
21	10267.0
22	11918.0
23	13474.0
24	15286.0
25	17254.0
26	19422.0
27	21844.0
28	24677.0
29	28087.0
30	32102.0
31	36693.0
32	43175.0
33	51790.0
34	63396.0
35	81684.0
36	115115.0
37	190015.0
38	466793.0
39	883167.0
40	962.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.056654765277973	20.643994844186434	19.142136310618387	31.157214079917207
2	20.302637989837823	49.6467831372822	12.540544302916407	17.510034569963572
3	37.547542498829564	18.80077599194869	23.076109294039423	20.575572215182326
4	17.480420401665388	17.167174668252304	23.82481810078708	41.527586829295224
5	18.41723440812518	34.699098960317414	26.28368806831179	20.599978563245617
6	17.383955006943747	27.465957552442326	31.10347781179922	24.04660962881471
7	20.644940067567536	26.71813034381863	35.84550132077319	16.791428267840647
8	21.125132645717727	27.90466861886518	33.777457721569974	17.192741013847122
9	20.925566670394126	28.21290316294207	27.634806793131144	23.22672337353266
10-11	19.502899297830297	30.264521197098894	28.80549950189706	21.427080003173753
12-13	24.18620373175846	26.63899531221798	27.217230881732686	21.95757007429088
14-15	21.682604259418138	27.278432351495958	28.623751088657684	22.415212300428227
16-17	21.181508525749855	29.421365391334447	28.267608646528803	21.129517436386898
18-19	21.426801603766187	29.636684133136164	29.640396125237046	19.296118137860603
20-21	22.815481048656327	29.050119781345106	29.681158438494844	18.453240731503723
22-23	22.025360330033216	30.1909077537483	28.59405515185064	19.189676764367846
24-25	22.15685765020692	28.21095436708911	26.757593459841118	22.874594522862857
26-27	20.256781693568556	26.515757174468735	30.08885117092471	23.138609961038004
28-29	19.942965082585093	28.077331218729277	30.52852882671491	21.451174871970714
30-31	20.236866351534744	30.10027283436131	28.150130129391567	21.512730684712388
32-33	20.70423914137911	30.072750405187136	28.599924739360155	20.6230857140736
34-35	21.402418455653528	29.253536948473375	29.77386544121434	19.57017915465876
36-37	21.908200579349167	27.845926343868737	28.900619299482134	21.34525377729996
38-39	21.43036778260908	29.066509709956435	29.05507203876837	20.448050468666118
40-41	21.001202701624205	29.47983154753177	29.94434720956519	19.574618541278834
42-43	20.525813693001123	29.890185200823694	29.41658074939657	20.167420356778617
44-45	20.78703976517938	29.95292266018058	28.67699497535469	20.58304259928535
46-47	19.928199127012007	27.959674766266673	28.855584844883854	23.25654126183747
48-49	20.925316325600246	28.11509869377288	30.293876162810797	20.665708817816082
50-51	21.730767356927064	30.648433980132488	28.300622176276008	19.320176486664437
52-53	20.402896792207475	30.146104027923908	28.383273959842565	21.067725220026055
54-55	22.19567127211434	28.335337416992186	28.1499577448513	21.319033566042176
56-57	21.613360242160887	28.675419305768955	29.1978026210584	20.51341783101176
58-59	21.053653728488285	27.874011874788707	29.158525343269748	21.913809053453257
60-61	19.599428816022847	30.316088387356466	29.635377214584913	20.449105582035777
62-63	20.419830944608027	31.06702584556699	27.44064151022198	21.072501699603
64-65	19.877569135321	30.07096173838279	28.945508150032328	21.105960976263884
66-67	20.366215860692648	30.182346971965647	29.460666107702522	19.990771059639183
68-69	20.853855622995233	29.990877779412084	29.216277827760784	19.938988769831898
70-71	19.819973023097408	30.773829713290368	29.146585176066747	20.259612087545477
72-73	20.081390066801937	30.795127267969175	28.442211474973984	20.681271190254908
74-75	20.13899554421748	28.89537611063964	30.431792841144834	20.533835503998045
76-77	19.640289405464145	30.395809717716922	28.55159924219681	21.41230163462212
78-79	19.599387892502566	29.33274157992592	28.548560048664218	22.5193104789073
80-81	20.603766335996927	29.401372466181925	28.463372868470096	21.53148832935105
82-83	20.261839282806008	28.17548164257506	29.897892377285025	21.664786697333906
84-85	20.670181613853526	28.806334700119756	28.558605627287225	21.96487805873949
86-87	21.417985622526594	29.33680157128626	28.784828345885284	20.46038446030187
88-89	21.5330573776539	28.910827277759555	28.872918558429305	20.68319678615724
90-91	21.06028878370547	30.044748064776115	28.3824484021498	20.512514749368613
92-93	21.231875618568683	29.316060815422585	29.501706820367886	19.950356745640846
94-95	21.022356864424594	30.01034253799108	28.292293393999657	20.675007203584673
96-97	20.866801195447056	29.72134075298688	28.992885503139647	20.418972548426417
98-99	21.69476103354852	30.57660693298045	28.117481765998804	19.61115026747223
100	21.187911712123878	31.49509297844214	27.072254390242655	20.244740919191322
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	9.0
2	14.0
3	15.0
4	25.0
5	29.0
6	27.0
7	31.5
8	41.0
9	43.0
10	43.0
11	61.0
12	78.0
13	90.5
14	110.0
15	134.0
16	174.5
17	258.0
18	376.0
19	578.5
20	954.5
21	1479.0
22	2354.5
23	3688.5
24	5521.0
25	8121.5
26	11742.5
27	16097.5
28	20810.5
29	26079.5
30	32449.5
31	39807.0
32	47867.5
33	56247.5
34	65724.0
35	76465.0
36	86430.0
37	94108.5
38	99141.5
39	102724.5
40	104545.0
41	104213.5
42	102199.0
43	100030.0
44	99016.0
45	96397.5
46	89469.0
47	80629.5
48	74316.0
49	71547.0
50	69326.0
51	64417.5
52	56788.5
53	48059.0
54	39808.0
55	32096.5
56	24632.0
57	18005.5
58	13111.5
59	10126.0
60	8392.5
61	7150.0
62	6028.0
63	4970.0
64	4155.0
65	4108.0
66	4097.5
67	3421.5
68	2600.0
69	2427.0
70	2646.5
71	2270.5
72	1441.5
73	812.5
74	481.0
75	312.5
76	217.5
77	166.0
78	122.5
79	89.5
80	74.5
81	57.0
82	36.0
83	24.0
84	20.0
85	18.5
86	15.0
87	14.0
88	14.0
89	12.0
90	14.0
91	11.0
92	9.0
93	13.0
94	10.5
95	10.0
96	7.0
97	11.0
98	34.0
99	75.5
100	76.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.408622122452124
2	0.005892787460148285
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	2.783994075660607E-4
30-31	0.009581579610398589
32-33	0.0
34-35	0.0
36-37	0.0
38-39	8.35198222698182E-4
40-41	0.0013455971365692934
42-43	1.3919970378303034E-4
44-45	0.0
46-47	0.002319995063050506
48-49	2.3199950630505056E-5
50-51	0.0
52-53	0.001600796593504849
54-55	0.019023959517014147
56-57	0.011414375710208488
58-59	0.012783172797408286
60-61	2.087995556745455E-4
62-63	0.012156774130384651
64-65	6.959985189151517E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	2.5519945693555564E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2155177.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.3836181799127
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.89940157868503	68.0197373000778
2	7.692477980959852	11.905435578719661
3	2.03625460387721	4.727182463505701
4	0.8587173386705726	2.658026186406175
5	0.4554045730069912	1.7620426797479594
6	0.24370352902569967	1.131519650353321
7	0.17231218132048584	0.9333898032936655
8	0.12308790687371163	0.7619990070463962
9	0.08932081167406063	0.6220770827494856
>10	0.4025055035379051	5.277375505205698
>50	0.01858797828536108	0.9611048804750969
>100	0.007860913635314595	1.0712052443675477
>500	3.651004478317661E-4	0.16890461805145038
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	9.279980252202024E-5	0.0
3	0.0	0.0	0.0	9.279980252202024E-5	0.0
4	0.0	0.0	0.0	9.279980252202024E-5	0.0
5	0.0	0.0	0.0	9.279980252202024E-5	0.0
6	0.0	0.0	0.0	9.279980252202024E-5	0.0
7	0.0	4.639990126101012E-5	0.0	9.279980252202024E-5	0.0
8	0.0	4.639990126101012E-5	0.0	9.279980252202024E-5	0.0
9	0.0	4.639990126101012E-5	0.0	9.279980252202024E-5	0.0
10-11	0.0	4.639990126101012E-5	0.0	9.279980252202024E-5	0.0
12-13	0.0	4.639990126101012E-5	0.0	9.279980252202024E-5	0.0
14-15	0.0	4.639990126101012E-5	0.0	9.279980252202024E-5	0.0
16-17	0.0	4.639990126101012E-5	0.0	9.279980252202024E-5	0.0
18-19	0.0	4.639990126101012E-5	0.0	9.279980252202024E-5	0.0
20-21	0.0	4.639990126101012E-5	0.0	9.279980252202024E-5	0.0
22-23	0.0	1.1599975315252529E-4	2.319995063050506E-5	9.279980252202024E-5	0.0
24-25	0.0	1.3919970378303034E-4	4.639990126101012E-5	9.279980252202024E-5	0.0
26-27	0.0	1.3919970378303034E-4	4.639990126101012E-5	9.279980252202024E-5	0.0
28-29	0.0	1.3919970378303034E-4	4.639990126101012E-5	9.279980252202024E-5	0.0
30-31	0.0	1.3919970378303034E-4	4.639990126101012E-5	9.279980252202024E-5	0.0
32-33	0.0	1.3919970378303034E-4	4.639990126101012E-5	9.279980252202024E-5	0.0
34-35	0.0	1.3919970378303034E-4	4.639990126101012E-5	9.279980252202024E-5	0.0
36-37	0.0	1.3919970378303034E-4	9.279980252202024E-5	9.279980252202024E-5	0.0
38-39	0.0	1.3919970378303034E-4	1.3919970378303034E-4	9.279980252202024E-5	0.0
40-41	0.0	1.3919970378303034E-4	1.3919970378303034E-4	9.279980252202024E-5	0.0
42-43	4.639990126101012E-5	1.3919970378303034E-4	1.3919970378303034E-4	9.279980252202024E-5	0.0
44-45	9.279980252202024E-5	1.3919970378303034E-4	1.3919970378303034E-4	9.279980252202024E-5	0.0
46-47	1.3919970378303034E-4	1.3919970378303034E-4	1.3919970378303034E-4	9.279980252202024E-5	0.0
48-49	1.3919970378303034E-4	1.3919970378303034E-4	1.623996544135354E-4	9.279980252202024E-5	0.0
50-51	1.3919970378303034E-4	1.3919970378303034E-4	1.8559960504404047E-4	9.279980252202024E-5	0.0
52-53	1.3919970378303034E-4	1.3919970378303034E-4	2.3199950630505058E-4	9.279980252202024E-5	0.0
54-55	2.087995556745455E-4	1.3919970378303034E-4	2.3199950630505058E-4	9.279980252202024E-5	0.0
56-57	2.3199950630505058E-4	1.3919970378303034E-4	4.175991113490911E-4	9.279980252202024E-5	0.0
58-59	2.5519945693555564E-4	1.3919970378303034E-4	6.031987163931316E-4	9.279980252202024E-5	0.0
60-61	2.783994075660607E-4	1.3919970378303034E-4	6.031987163931316E-4	9.279980252202024E-5	0.0
62-63	3.015993581965658E-4	1.3919970378303034E-4	6.031987163931316E-4	9.279980252202024E-5	0.0
64-65	3.4799925945757585E-4	1.3919970378303034E-4	6.031987163931316E-4	9.279980252202024E-5	0.0
66-67	4.6399901261010116E-4	1.3919970378303034E-4	6.031987163931316E-4	9.279980252202024E-5	0.0
68-69	5.799987657626265E-4	1.3919970378303034E-4	6.031987163931316E-4	9.279980252202024E-5	0.0
70-71	6.959985189151517E-4	1.3919970378303034E-4	6.031987163931316E-4	9.279980252202024E-5	0.0
72-73	6.959985189151517E-4	1.3919970378303034E-4	6.031987163931316E-4	9.279980252202024E-5	0.0
74-75	6.959985189151517E-4	1.3919970378303034E-4	6.031987163931316E-4	9.279980252202024E-5	0.0
76-77	7.65598370806667E-4	1.3919970378303034E-4	6.031987163931316E-4	9.279980252202024E-5	0.0
78-79	0.0010207978277422226	1.3919970378303034E-4	6.031987163931316E-4	9.279980252202024E-5	0.0
80-81	0.001531196741613334	1.3919970378303034E-4	6.031987163931316E-4	9.279980252202024E-5	0.0
82-83	0.0017399962972878794	1.3919970378303034E-4	6.031987163931316E-4	9.279980252202024E-5	0.0
84-85	0.0018095961491793947	1.3919970378303034E-4	6.031987163931316E-4	9.279980252202024E-5	0.0
86-87	0.002041595655484445	2.3199950630505058E-4	6.031987163931316E-4	9.279980252202024E-5	0.0
88	0.002041595655484445	2.3199950630505058E-4	6.031987163931316E-4	9.279980252202024E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	8400	0.0	79.62718	8
CACCGGC	8290	0.0	77.22992	9
GCAGCCT	110	0.0	77.05476	1
CTTCACC	9205	0.0	74.34632	6
CGTAGTG	1905	0.0	71.94715	4
GTGTGGC	3350	0.0	70.47724	7
CCTTCAC	10270	0.0	70.24722	5
CGGGGCA	940	0.0	68.909164	94
GCTGCCA	90	0.0	67.80266	2
TTCACCG	10310	0.0	66.6057	7
CGGGCCC	545	0.0	65.455	7
AAGCTGC	25	0.0032600942	63.570183	1
TGTGGCA	3570	0.0	63.110085	8
GGGCCCG	570	0.0	62.584167	8
GCGTAGT	1295	0.0	61.98007	3
TGTGTGG	3715	0.0	61.278576	6
CAGCGTA	750	0.0	60.038506	1
ACCTTCA	4085	0.0	59.060455	4
ATGCAGC	40	8.097075E-6	58.67538	2
AGGCGCG	340	0.0	57.982388	5
>>END_MODULE