FastQCFastQC Report
Tue 7 Mar 2017
1SN_20A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_20A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2155177
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG84000.079.627188
CACCGGC82900.077.229929
GCAGCCT1100.077.054761
CTTCACC92050.074.346326
CGTAGTG19050.071.947154
GTGTGGC33500.070.477247
CCTTCAC102700.070.247225
CGGGGCA9400.068.90916494
GCTGCCA900.067.802662
TTCACCG103100.066.60577
CGGGCCC5450.065.4557
AAGCTGC250.003260094263.5701831
TGTGGCA35700.063.1100858
GGGCCCG5700.062.5841678
GCGTAGT12950.061.980073
TGTGTGG37150.061.2785766
CAGCGTA7500.060.0385061
ACCTTCA40850.059.0604554
ATGCAGC408.097075E-658.675382
AGGCGCG3400.057.9823885
Produced by FastQC (version 0.11.5)