FastQCFastQC Report
Tue 7 Mar 2017
1SN_21A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_21A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3540618
Sequences flagged as poor quality0
Sequence length94
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA177180.5004211129243539No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG99860.28204115778657846No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG81970.23151325559549207No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT73210.2067718121525677No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA70160.1981574967985815No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG61550.17383970820913186No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG59180.16714596152423108No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA58950.1664963574155698No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT56210.15875759542543139No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC49550.13994731993115325No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC48830.13791377663447454No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA48690.1375183654378981No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA46870.13237801988240472No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA44490.12565602954060562No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT42550.12017676010233243No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA40480.1143303231243811No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA38880.10981133802065064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTA600.087.999613
GCTGCCG158.9721585E-487.999613
AGCTGCA1600.087.999612
GCAGCTA158.9721585E-487.999613
GCAGCCT158.9721585E-487.999613
GCTGCAT500.087.99963
GCAGCAT550.087.99963
GCAGCAC356.0754246E-1087.99963
AGCAGCG1300.087.99962
CAGCAGC3900.086.871411
AGCAGCA1600.085.249622
CAGCTGC5900.085.016571
CTGCTGC1450.084.965131
AGCTGCC2050.083.706942
CAGCCCT196150.082.48141
CTGCAGC1500.082.1329651
CAGCGTA79950.081.7257161
AGCTGCT1400.081.713922
GCTGCCC1250.080.959633
AGCCCTT167300.080.530422
Produced by FastQC (version 0.11.5)