FastQCFastQC Report
Tue 7 Mar 2017
1SN_21A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_21A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3540618
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG131800.079.809988
CACCGGC126900.079.488729
CTTCACC146850.074.091826
CCTTCAC160550.071.248545
TTCACCG158350.068.2070547
GTGTGGC50400.067.614037
CGTAGTG30000.065.557674
GGCCCGA11800.064.0434349
CGGGGCA16450.063.6311494
CGGGCCC12250.063.223517
GGGCCCG12200.063.097888
AACGGGC12400.062.837255
CGCTGCC950.061.2547341
CGCTGCA1150.059.802051
ACCTTCA64250.059.1755944
ACGGGCC13150.058.896436
GCCTTCA51050.057.374594
CAGCGTA12850.057.224531
TGTGGCA60650.057.1932038
TGTGTGG56600.055.5634426
Produced by FastQC (version 0.11.5)