##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1SN_21A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3540618
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.821111173247155	12.0	12.0	12.0	2.0	32.0
2	14.660668278814603	12.0	12.0	12.0	12.0	22.0
3	13.698843252788073	12.0	12.0	12.0	12.0	22.0
4	13.818650020985038	12.0	12.0	12.0	12.0	22.0
5	22.441037412112802	22.0	22.0	22.0	22.0	27.0
6	26.886783606703688	27.0	22.0	32.0	22.0	32.0
7	34.99404426006985	37.0	37.0	37.0	27.0	41.0
8	37.61224509393558	41.0	37.0	41.0	27.0	41.0
9	38.54521470545537	41.0	41.0	41.0	32.0	41.0
10-11	38.900242838962015	41.0	41.0	41.0	37.0	41.0
12-13	39.08416736852154	41.0	41.0	41.0	37.0	41.0
14-15	39.04058980663828	41.0	41.0	41.0	37.0	41.0
16-17	38.94336652527892	41.0	41.0	41.0	37.0	41.0
18-19	38.88150147799057	41.0	41.0	41.0	37.0	41.0
20-21	38.8987960576374	41.0	41.0	41.0	37.0	41.0
22-23	38.88257275989672	41.0	41.0	41.0	37.0	41.0
24-25	38.90681231355656	41.0	41.0	41.0	37.0	41.0
26-27	38.873718655895665	41.0	41.0	41.0	37.0	41.0
28-29	38.823407241334706	41.0	41.0	41.0	37.0	41.0
30-31	38.696816911623905	41.0	41.0	41.0	32.0	41.0
32-33	38.744724790982815	41.0	41.0	41.0	32.0	41.0
34-35	38.71208613863456	41.0	41.0	41.0	32.0	41.0
36-37	38.639911732923466	41.0	41.0	41.0	32.0	41.0
38-39	38.550613904126344	41.0	41.0	41.0	32.0	41.0
40-41	38.59191742797444	41.0	41.0	41.0	32.0	41.0
42-43	38.33017470396411	41.0	41.0	41.0	32.0	41.0
44-45	38.44354092986027	41.0	41.0	41.0	32.0	41.0
46-47	38.454152071756965	41.0	41.0	41.0	32.0	41.0
48-49	38.401736928411935	41.0	41.0	41.0	32.0	41.0
50-51	38.282135915255466	41.0	41.0	41.0	32.0	41.0
52-53	38.264263046733646	41.0	41.0	41.0	32.0	41.0
54-55	38.12747463860829	41.0	39.0	41.0	32.0	41.0
56-57	37.914618577886685	41.0	37.0	41.0	29.5	41.0
58-59	37.921093859885474	41.0	37.0	41.0	29.5	41.0
60-61	37.92976042600472	41.0	37.0	41.0	32.0	41.0
62-63	37.925506648839274	41.0	37.0	41.0	32.0	41.0
64-65	37.896587968541084	41.0	37.0	41.0	29.5	41.0
66-67	37.779644965935326	41.0	37.0	41.0	27.0	41.0
68-69	37.78503201983382	41.0	37.0	41.0	27.0	41.0
70-71	37.76587816025338	41.0	37.0	41.0	27.0	41.0
72-73	37.62663255397786	41.0	37.0	41.0	27.0	41.0
74-75	37.53062219646401	41.0	37.0	41.0	27.0	41.0
76-77	36.49162886253191	39.0	34.5	41.0	24.5	41.0
78-79	37.011249024887746	41.0	37.0	41.0	27.0	41.0
80-81	37.649154469643435	41.0	37.0	41.0	27.0	41.0
82-83	37.84007594154467	41.0	37.0	41.0	32.0	41.0
84-85	37.75386825124879	41.0	37.0	41.0	27.0	41.0
86-87	37.609640887551265	41.0	37.0	41.0	27.0	41.0
88-89	37.53207843941368	41.0	37.0	41.0	27.0	41.0
90-91	37.373145450878916	41.0	37.0	41.0	27.0	41.0
92-93	37.27918388823646	41.0	37.0	41.0	27.0	41.0
94-95	37.11680955132692	41.0	37.0	41.0	27.0	41.0
96-97	37.06360287949731	41.0	37.0	41.0	27.0	41.0
98-99	36.93799119249803	41.0	37.0	41.0	27.0	41.0
100	36.79721732194775	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.454936004384578
1101	2	-0.5640350681156168
1101	3	-0.023205302485306945
1101	4	0.03502487550602673
1101	5	-1.0650081247652956
1101	6	-0.8051015112116993
1101	7	-0.3084966918815013
1101	8	-0.039313439518814164
1101	9	-0.2714364063552068
1101	10-11	-0.03141800327483679
1101	12-13	-0.013192488145286063
1101	14-15	-0.04288767573503094
1101	16-17	0.033774125030646474
1101	18-19	-0.029522028877416062
1101	20-21	0.030160176900942304
1101	22-23	0.08903182863748782
1101	24-25	-0.04742331629455521
1101	26-27	0.08309124410395441
1101	28-29	0.007500074223315778
1101	30-31	-0.08334030644424928
1101	32-33	-0.1699858568478163
1101	34-35	-0.01969858051803186
1101	36-37	-0.06806189634259141
1101	38-39	-0.06700262451964534
1101	40-41	0.021399688754719648
1101	42-43	0.05944416878540437
1101	44-45	-0.01569808059341682
1101	46-47	-0.1288866359394305
1101	48-49	0.03742663096344501
1101	50-51	0.05141004849343034
1101	52-53	0.03464011274019185
1101	54-55	-0.18395933196141812
1101	56-57	0.1223974519935851
1101	58-59	0.052984353078002755
1101	60-61	0.0024308214465591504
1101	62-63	-0.1827655643530548
1101	64-65	-0.03857521873786851
1101	66-67	-0.04767189413490769
1101	68-69	-0.08691697245679819
1101	70-71	-0.21715632467931556
1101	72-73	-0.05891938010455533
1101	74-75	0.030586101463619286
1101	76-77	-0.46745168583751706
1101	78-79	-0.08259516382477727
1101	80-81	-0.032837998178465
1101	82-83	-0.11327423403918147
1101	84-85	-0.03463234609463939
1101	86-87	-0.18920189859655068
1101	88-89	-0.4179791138443889
1101	90-91	-0.1646567475409384
1101	92-93	0.02420021327201738
1101	94-95	0.1527221961368852
1101	96-97	-0.16278061813372346
1101	98-99	-0.3406657850903301
1101	100	-0.48731897291481374
1102	1	1.639659540959057
1102	2	0.7852308035810953
1102	3	-0.12290690396182669
1102	4	-0.1095483495695575
1102	5	-0.8200285706667643
1102	6	-0.2427930329613197
1102	7	-0.11240731358064693
1102	8	-0.45293693626402387
1102	9	-0.3856510457573563
1102	10-11	-0.42545913575180805
1102	12-13	-0.31579980401463104
1102	14-15	-0.3515789495614001
1102	16-17	-0.4103040613061566
1102	18-19	-0.28145461860668775
1102	20-21	-0.2350911420942623
1102	22-23	-0.27477488691376806
1102	24-25	-0.3479976925459596
1102	26-27	-0.24207869309917385
1102	28-29	-0.3421681808505639
1102	30-31	-0.29887105172392836
1102	32-33	-0.23988633751238808
1102	34-35	-0.18596099972290148
1102	36-37	-0.39555613994571814
1102	38-39	-0.3524524395299551
1102	40-41	-0.2941691770554158
1102	42-43	-0.14222985169508462
1102	44-45	-0.2200586094715007
1102	46-47	-0.47008143547473225
1102	48-49	-0.4606924040952265
1102	50-51	-0.21888802070842672
1102	52-53	-0.3015331017308256
1102	54-55	-0.3244522311453011
1102	56-57	-0.24868633923142625
1102	58-59	-0.347419107325841
1102	60-61	-0.2492621816289713
1102	62-63	-0.38619068521305877
1102	64-65	-0.4192927028367066
1102	66-67	-0.34508198674470236
1102	68-69	-0.4464247464145856
1102	70-71	-0.597354350530857
1102	72-73	-0.28653643581603916
1102	74-75	-0.4841674033779313
1102	76-77	-0.5028961674946189
1102	78-79	-0.5337265285604786
1102	80-81	-0.32355152213308713
1102	82-83	-0.3838349320518759
1102	84-85	-0.3547906917982431
1102	86-87	-0.3319293692180523
1102	88-89	-0.4140558370818823
1102	90-91	-0.46443645416301393
1102	92-93	-0.29867135937539757
1102	94-95	-0.29376806391741894
1102	96-97	-0.38193695621819046
1102	98-99	-0.4026837250407098
1102	100	-0.5289932771504056
1103	1	1.322451853858201
1103	2	0.6384765248688424
1103	3	0.4112749035181942
1103	4	1.1860165581675748
1103	5	2.831359318644548
1103	6	-0.05002391620739033
1103	7	-0.46181606604540804
1103	8	-0.21379618355312147
1103	9	-0.10554595410984291
1103	10-11	-0.2006116497377164
1103	12-13	-0.32724344968882946
1103	14-15	-0.4880543661957404
1103	16-17	-0.49467804630759105
1103	18-19	-0.23439758605449157
1103	20-21	-0.14436735280195023
1103	22-23	-0.11549078363072596
1103	24-25	-0.16062768593759813
1103	26-27	-0.24251468431300083
1103	28-29	-0.058523148758553134
1103	30-31	-0.04448591009902003
1103	32-33	-0.4022501504556786
1103	34-35	-0.23482519124226542
1103	36-37	-0.2801825268317373
1103	38-39	-0.13913308987816464
1103	40-41	-0.2032330105996678
1103	42-43	-0.15703182831700957
1103	44-45	-0.6358833568422071
1103	46-47	-0.4381787897229472
1103	48-49	-0.3227311499516219
1103	50-51	-0.21655979124049907
1103	52-53	-0.2049653820965034
1103	54-55	-0.28872358591254965
1103	56-57	-0.37803195109329124
1103	58-59	-0.5565396603947121
1103	60-61	-0.40245145093702206
1103	62-63	-0.3299078474175232
1103	64-65	-0.4366736520326029
1103	66-67	-0.46799512293310386
1103	68-69	-0.31074516892153525
1103	70-71	-0.45987333953208065
1103	72-73	-0.2465139085331458
1103	74-75	-0.4178028306070516
1103	76-77	-0.4204227681490309
1103	78-79	-0.49960229674886136
1103	80-81	-0.33821143239852347
1103	82-83	-0.4322866228020459
1103	84-85	-0.6030878485983067
1103	86-87	-0.44399216736344016
1103	88-89	-0.2972373743415275
1103	90-91	-0.644352712600714
1103	92-93	-0.21780451381035704
1103	94-95	-0.4191478038102403
1103	96-97	-0.25037826622516945
1103	98-99	-0.07393730679842747
1103	100	-0.21632432921732914
1104	1	1.5633667057996696
1104	2	-0.7044494511024144
1104	3	-0.031217947564355697
1104	4	-0.0031469399312840096
1104	5	-1.1651798720875206
1104	6	-0.5980067169070935
1104	7	-0.28627049627930035
1104	8	-0.016589760525754116
1104	9	0.06815074324741488
1104	10-11	0.018266782004531024
1104	12-13	0.05485995560503909
1104	14-15	0.09511561568319848
1104	16-17	0.10304351776032661
1104	18-19	-0.01883373810385791
1104	20-21	-0.010899839787256838
1104	22-23	-0.08123186188649356
1104	24-25	-0.0704148943507974
1104	26-27	-0.03567440107970299
1104	28-29	-0.10277672845457175
1104	30-31	-0.12720155601549266
1104	32-33	-0.15287407026210786
1104	34-35	-0.12743383907560002
1104	36-37	-0.24370424592380147
1104	38-39	-0.14234916001611708
1104	40-41	-0.05683596024824311
1104	42-43	-0.0675457647780604
1104	44-45	-0.13269881526326088
1104	46-47	-0.3438009812196654
1104	48-49	-0.4105215845429555
1104	50-51	-0.29991114089326487
1104	52-53	-0.1684550488993466
1104	54-55	-0.22796836694094225
1104	56-57	-0.18317807182358337
1104	58-59	-0.07021394209872511
1104	60-61	-0.03875721511177943
1104	62-63	-0.11856861688195153
1104	64-65	-0.29979717904137004
1104	66-67	-0.6164726915679424
1104	68-69	-0.4996436071580277
1104	70-71	-0.27285074581056534
1104	72-73	-0.1506299566744218
1104	74-75	-0.23081040374596995
1104	76-77	-0.4090931742494419
1104	78-79	-0.3409717988909051
1104	80-81	-0.34498541138646743
1104	82-83	-0.31738686476540323
1104	84-85	-0.23615690766459352
1104	86-87	-0.2349101616862015
1104	88-89	-0.23209182971447717
1104	90-91	-0.3349879449798081
1104	92-93	-0.4560090095105309
1104	94-95	-0.43325515802993664
1104	96-97	-0.2963938314871939
1104	98-99	-0.5270657568712238
1104	100	-0.4306447892619474
1105	1	1.4730963160459503
1105	2	0.40192934899236477
1105	3	-0.03227943765791075
1105	4	-0.12797340419127146
1105	5	-1.1437493014908746
1105	6	0.664165036165663
1105	7	0.10395671825937569
1105	8	0.055504499794558626
1105	9	0.11875281088699552
1105	10-11	0.009944620158691464
1105	12-13	-0.03831840017464572
1105	14-15	-0.14088662974603494
1105	16-17	-0.0029983486811602234
1105	18-19	0.06512873682986964
1105	20-21	-0.04246942980559254
1105	22-23	0.04255716477081961
1105	24-25	0.01630725604525196
1105	26-27	-0.06984802110282118
1105	28-29	-0.044173728930061884
1105	30-31	-0.036000474011210315
1105	32-33	-0.14399989381144707
1105	34-35	-0.1067204700253015
1105	36-37	-0.10940746124487788
1105	38-39	-0.014798602077846112
1105	40-41	0.09886177667078044
1105	42-43	0.1076418446508356
1105	44-45	0.06025583247310351
1105	46-47	-0.24986864942410136
1105	48-49	-0.08893183018845718
1105	50-51	-0.09419555344795327
1105	52-53	-0.14479356007798572
1105	54-55	-0.11955464660502457
1105	56-57	8.83714055973428E-4
1105	58-59	-0.08913669265411528
1105	60-61	-0.06103995949701613
1105	62-63	-0.3258903541317508
1105	64-65	-0.17127935316390364
1105	66-67	-0.1197378669006639
1105	68-69	-0.10888525001073646
1105	70-71	-0.3000070019230776
1105	72-73	-0.08699541459409943
1105	74-75	-0.27997409267451445
1105	76-77	-0.2993826072149517
1105	78-79	-0.09819609373548843
1105	80-81	-0.31389726874763824
1105	82-83	-0.12183892119374917
1105	84-85	0.026861652112003753
1105	86-87	-0.11637491464922789
1105	88-89	-0.024441507609878954
1105	90-91	-0.06193870138095292
1105	92-93	-0.1424193919173078
1105	94-95	-0.015171580991200528
1105	96-97	0.07540042867408658
1105	98-99	-0.07835143208228601
1105	100	-0.12279000110674332
1106	1	1.3223376823154585
1106	2	-0.6877997962126727
1106	3	-0.025999510379596913
1106	4	-0.23470557684027504
1106	5	-1.1114485228645918
1106	6	-0.3302937762175091
1106	7	-0.1706672148965538
1106	8	-0.12910684691573238
1106	9	-0.21343027757858124
1106	10-11	-0.03754562485350732
1106	12-13	-0.0023223967158330083
1106	14-15	-0.030619074214996544
1106	16-17	-0.0284839806330055
1106	18-19	-0.08907511631644383
1106	20-21	-0.09754300831527019
1106	22-23	-0.20271888020946705
1106	24-25	-0.13934507244719185
1106	26-27	-0.2089614842662968
1106	28-29	-0.07420564807740959
1106	30-31	-0.030039836691905464
1106	32-33	-0.13447792268345182
1106	34-35	-0.0661113077231903
1106	36-37	-0.2969714521920892
1106	38-39	-0.0813179180370156
1106	40-41	-0.10346991655085702
1106	42-43	-0.08330866836008255
1106	44-45	-0.1389582040728925
1106	46-47	-0.3087025516752817
1106	48-49	-0.24750533680372655
1106	50-51	-0.18287996457366518
1106	52-53	-0.17309543386291892
1106	54-55	-0.2419934329486324
1106	56-57	-0.20138470223825067
1106	58-59	-0.24816180448438985
1106	60-61	-0.12050352872936543
1106	62-63	-0.12940367626399762
1106	64-65	-0.29994700205919145
1106	66-67	-0.37170102741822575
1106	68-69	-0.48359777086503897
1106	70-71	-0.5452570467339726
1106	72-73	-0.49229851353852894
1106	74-75	-0.3503775286362938
1106	76-77	-0.41756588477266376
1106	78-79	-0.35306364059981377
1106	80-81	-0.34057841606420425
1106	82-83	-0.46700618895018664
1106	84-85	-0.5477582431388299
1106	86-87	-0.5363296999607101
1106	88-89	-0.4701198165746163
1106	90-91	-0.668087938413862
1106	92-93	-0.5138780506631164
1106	94-95	-0.5546281676022957
1106	96-97	-0.38835691095706437
1106	98-99	-0.6492887086433399
1106	100	-0.6003387563486342
1107	1	1.3662658018765477
1107	2	-0.6411477788528384
1107	3	-0.10503164069614712
1107	4	-0.10796791789969618
1107	5	-1.220691294508029
1107	6	-0.2916053901256852
1107	7	-0.3196109619644929
1107	8	-0.317907032130627
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2225	1	1.379298076284936
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2225	4	1.0268896995785202
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2226	1	1.5021911291123224
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2226	3	0.8021103817136908
2226	4	1.028720285268017
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2226	100	0.36006520211506654
2227	1	1.3993258570479945
2227	2	1.7983431335392783
2227	3	0.877883573954616
2227	4	-0.20154325044486576
2227	5	3.2940532435394303
2227	6	0.28685298679391735
2227	7	0.3478809036515642
2227	8	0.5465923911895203
2227	9	0.4231476808038863
2227	10-11	0.3150386329939039
2227	12-13	0.3414588846594384
2227	14-15	0.3003376883207167
2227	16-17	0.3041678287105327
2227	18-19	0.3319675002148941
2227	20-21	0.35586173230751683
2227	22-23	0.3600667051325104
2227	24-25	0.49813699435819103
2227	26-27	0.3899023085824638
2227	28-29	0.39441871002063067
2227	30-31	0.4014993008622696
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2227	48-49	0.27810689557541934
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2227	86-87	0.29048659151723655
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2227	90-91	0.4877134031017718
2227	92-93	0.5134467548571777
2227	94-95	0.5426732778194534
2227	96-97	0.47608541363563717
2227	98-99	0.60579881035396
2227	100	0.6183458172231582
2228	1	1.2368328412264447
2228	2	1.6627139823840569
2228	3	-0.05197122949932442
2228	4	0.032457341506088255
2228	5	3.1725431637648676
2228	6	0.346186402425289
2228	7	0.3712491839051424
2228	8	0.4215419092750068
2228	9	0.17554391710634576
2228	10-11	0.33928214793323264
2228	12-13	0.2941579380832593
2228	14-15	0.2955072396327978
2228	16-17	0.22350464533315062
2228	18-19	0.18708904103248614
2228	20-21	0.18055109439360706
2228	22-23	0.25377070684985625
2228	24-25	0.22922066617938697
2228	26-27	0.21677668360074165
2228	28-29	0.2692547460513026
2228	30-31	0.25986997817017254
2228	32-33	0.22259456653078757
2228	34-35	0.2881230409025619
2228	36-37	0.41497840797482866
2228	38-39	0.287248503773327
2228	40-41	0.1900820486028607
2228	42-43	0.44202627468435196
2228	44-45	0.2147376981420379
2228	46-47	0.3490012481440701
2228	48-49	0.36393750340592845
2228	50-51	0.30921711193658297
2228	52-53	0.30641383523291665
2228	54-55	0.31118398158142213
2228	56-57	0.4355041959766339
2228	58-59	0.41763508790781856
2228	60-61	0.2673045200538624
2228	62-63	0.4747010870396693
2228	64-65	0.2831178481844816
2228	66-67	0.38405213633262747
2228	68-69	0.44357791325811036
2228	70-71	0.5587662225053336
2228	72-73	0.6284041019329436
2228	74-75	0.43765240847664444
2228	76-77	0.47784238829003556
2228	78-79	0.5253497529252655
2228	80-81	0.46031308996073506
2228	82-83	0.3041143153955943
2228	84-85	0.38120489372875
2228	86-87	0.3049932073375956
2228	88-89	0.5395494785602821
2228	90-91	0.42862955875214226
2228	92-93	0.5579514520425377
2228	94-95	0.7996566878238198
2228	96-97	0.5691261229059137
2228	98-99	0.6026268654579496
2228	100	0.8394720212657703
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	19.0
13	39.0
14	204.0
15	567.0
16	2234.0
17	5776.0
18	9482.0
19	12199.0
20	14108.0
21	16298.0
22	18840.0
23	21617.0
24	24554.0
25	27816.0
26	31139.0
27	35496.0
28	40203.0
29	45852.0
30	52296.0
31	60441.0
32	70991.0
33	84998.0
34	103959.0
35	134585.0
36	187870.0
37	310446.0
38	761509.0
39	1465227.0
40	1853.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.51320561142562	18.695401177065843	19.10176687243932	34.68962633906922
2	18.534639202920552	49.749027306043764	15.180352641278944	16.535980849756736
3	40.26565418805418	16.210672826043364	24.001826799728182	19.521846186174276
4	18.346740597262965	16.14401779576334	22.171835538315626	43.33740606865807
5	17.521404455380388	34.31920642102593	26.687855058071786	21.471534065521894
6	17.495194341778753	28.832311195390183	29.63378144719368	24.038713015637384
7	20.370991730822134	26.169019080849726	35.768303725507806	17.691685462820335
8	21.503929539984263	28.26579992532377	33.120715084202814	17.109555450489154
9	21.000712305026976	28.531996391590397	27.135827700135966	23.331463603246664
10-11	19.658220683507793	30.390937401323725	28.64988541548396	21.300956499684517
12-13	24.121692879604634	26.69478887584032	27.378087667181266	21.80543057737378
14-15	21.835524193798935	27.446846849900215	28.580970892652076	22.136658063648778
16-17	20.722865330289796	29.67241876983058	28.479491433416428	21.125224466463198
18-19	21.284899980737826	29.502406077131166	29.82832375590928	19.384370186221727
20-21	22.740606300933905	28.86087965434283	29.877340622456305	18.52117342226696
22-23	21.737617557160924	30.21397676902733	28.83050360134869	19.217902072463055
24-25	22.269742174953638	28.300610232450946	26.793076801846453	22.63657079074896
26-27	20.212657790250177	26.692472895974657	30.031748694719397	23.06312061905577
28-29	19.604200523865124	28.29123037129802	30.594437788333412	21.510131316503443
30-31	20.28033804847467	29.933932846486055	28.31536596943474	21.47036313560453
32-33	20.67617856543688	30.02698963853203	28.670121995651606	20.62670980037948
34-35	21.268631634364397	29.18206652059047	30.019971089792797	19.52933075525233
36-37	21.70667663102882	28.1739656749189	28.78946556787544	21.32989212617684
38-39	21.329874211660456	29.22174987477371	29.074246230960625	20.374129682605204
40-41	21.049419189237433	29.48182325547765	30.030859455319032	19.43789809996588
42-43	20.35776845484992	29.872191659412746	29.399590014613285	20.37044987112405
44-45	20.480788947014336	30.260804752164734	28.793377314355855	20.465028986465075
46-47	20.054745793445417	28.256051467767968	28.7926353731882	22.896567365598415
48-49	20.84377936899425	28.1330022234822	30.295026220708678	20.72819218681487
50-51	21.68251418255231	30.741003406749893	28.236708958718502	19.339773451979287
52-53	20.389776112384133	30.182292630200042	28.37296582952298	21.054965427892846
54-55	22.088067178653613	28.50202856889422	28.254410957944415	21.155493294507753
56-57	21.510861131017435	28.61588337133032	29.348738896438697	20.52451660121355
58-59	21.04663269808431	28.097481620752205	29.239119197182934	21.616766483980552
60-61	19.663755728002275	30.307245035849046	29.60242308494538	20.426576151203303
62-63	20.53866875243013	31.005153536229198	27.545862291873842	20.910315419466833
64-65	19.60069360841176	30.288640573550158	28.904092778245133	21.20657303979295
66-67	20.15878301471664	30.29944207480163	29.54888101455734	19.992893895924386
68-69	20.778293506952743	29.98876184892016	29.301763138525533	19.931181505601565
70-71	19.68275029952398	31.00505335509225	29.003255928767235	20.308940416616533
72-73	20.10812801606951	30.826129788641417	28.516335283840277	20.54940691144879
74-75	20.169515604338002	29.232650910095355	30.342626626199156	20.25520685936749
76-77	19.508388083662233	30.67402357441554	28.719731978993497	21.09785636292873
78-79	19.650849089057335	29.43031414289822	28.564956739190727	22.35388002885372
80-81	20.58433973905355	29.58700778100054	28.555589903043582	21.273062576902323
82-83	20.479292033198725	28.383392390819907	29.612400998921657	21.52491457705971
84-85	20.597308153548337	29.169625189726766	28.427254789982992	21.80581186674191
86-87	21.345426137470916	28.299946506513834	30.066784951101756	20.28784240491349
88-89	20.65057569045856	27.959440978947743	29.9981104993535	21.391872831240196
90-91	20.197956966834603	28.97059213956434	29.546889836746015	21.284561056855047
92-93	20.06168132230023	29.44889846913731	29.541156374395655	20.948263834166802
94-95	20.253611092752735	29.47397883646301	29.27467747156005	20.997732599224204
96-97	20.0580237687319	29.286257370888357	29.627483111705356	21.028235748674383
98-99	21.217129326010316	29.681654445636322	28.74536309762872	20.355853130724636
100	20.354186754967635	30.85899128344261	28.02685858796402	20.759963373625734
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	11.5
2	16.0
3	16.0
4	22.5
5	30.5
6	46.0
7	57.0
8	70.5
9	85.5
10	95.0
11	106.5
12	121.0
13	153.5
14	198.0
15	233.5
16	297.5
17	425.0
18	611.0
19	960.5
20	1525.5
21	2435.0
22	3827.0
23	6000.0
24	9227.5
25	13424.0
26	19074.0
27	25673.5
28	33240.0
29	42652.5
30	53131.5
31	64786.0
32	77870.5
33	92085.0
34	107963.0
35	125712.5
36	142383.0
37	154591.0
38	162879.0
39	168768.0
40	172277.5
41	172938.0
42	171307.5
43	167154.0
44	163492.5
45	159275.5
46	148804.0
47	135616.0
48	125309.5
49	118499.5
50	112787.5
51	105297.5
52	93505.5
53	78912.5
54	65377.0
55	53093.5
56	41629.0
57	31134.0
58	22316.5
59	16459.0
60	13253.5
61	11363.5
62	9366.0
63	7101.5
64	5406.0
65	4406.0
66	4111.0
67	3712.5
68	2849.0
69	2638.0
70	2890.5
71	2595.0
72	1724.5
73	918.5
74	474.0
75	298.0
76	233.0
77	172.0
78	140.0
79	114.5
80	85.0
81	61.0
82	45.0
83	35.5
84	35.5
85	28.0
86	24.0
87	23.5
88	19.0
89	16.0
90	20.5
91	21.0
92	15.5
93	16.5
94	14.5
95	15.5
96	21.5
97	18.0
98	42.0
99	114.0
100	133.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.300202394045334
2	0.00545102578137489
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	3.389238827797859E-4
30-31	0.010068863684249472
32-33	0.0
34-35	0.0
36-37	0.0
38-39	7.767005647036759E-4
40-41	0.0014121828449157746
42-43	1.1297462759326197E-4
44-45	0.0
46-47	0.002033543296678715
48-49	1.4121828449157746E-5
50-51	0.0
52-53	0.001327451874220828
54-55	0.019318661318447797
56-57	0.012003554181784084
58-59	0.013669929938784698
60-61	2.118274267373662E-4
62-63	0.012074163324029873
64-65	9.885279914410421E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.835837698390507E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3540618.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.58920749592022
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.1752409811362	61.828032410947046
2	9.089266422486153	13.19565292655096
3	2.5981687372381352	5.657970287303761
4	1.105805004797531	3.2107803577310015
5	0.5815548117715589	2.110730145096826
6	0.3515906917839361	1.5313013807722966
7	0.23221490293166572	1.179940704078616
8	0.1706821697089447	0.9911746746285166
9	0.11501568738698263	0.751400783731762
>10	0.5428468651599924	6.592412619633957
>50	0.026876112646711733	1.3283498861757557
>100	0.01006460812529859	1.2726395772762096
>500	5.938277910431485E-4	0.2760188110711473
>1k	7.917703582068368E-5	0.07359543500228431
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.824365689831549E-5	0.0
3	0.0	0.0	0.0	2.824365689831549E-5	0.0
4	0.0	0.0	0.0	2.824365689831549E-5	0.0
5	0.0	0.0	0.0	2.824365689831549E-5	0.0
6	0.0	0.0	0.0	2.824365689831549E-5	0.0
7	0.0	0.0	0.0	2.824365689831549E-5	0.0
8	0.0	0.0	0.0	2.824365689831549E-5	0.0
9	5.648731379663098E-5	0.0	0.0	2.824365689831549E-5	0.0
10-11	5.648731379663098E-5	0.0	0.0	2.824365689831549E-5	0.0
12-13	5.648731379663098E-5	0.0	0.0	2.824365689831549E-5	0.0
14-15	5.648731379663098E-5	0.0	0.0	2.824365689831549E-5	0.0
16-17	5.648731379663098E-5	0.0	0.0	2.824365689831549E-5	0.0
18-19	5.648731379663098E-5	0.0	0.0	2.824365689831549E-5	0.0
20-21	5.648731379663098E-5	0.0	0.0	2.824365689831549E-5	0.0
22-23	5.648731379663098E-5	0.0	0.0	2.824365689831549E-5	0.0
24-25	5.648731379663098E-5	0.0	0.0	2.824365689831549E-5	0.0
26-27	5.648731379663098E-5	0.0	0.0	2.824365689831549E-5	0.0
28-29	5.648731379663098E-5	0.0	0.0	2.824365689831549E-5	0.0
30-31	5.648731379663098E-5	0.0	0.0	2.824365689831549E-5	0.0
32-33	5.648731379663098E-5	0.0	0.0	2.824365689831549E-5	0.0
34-35	5.648731379663098E-5	0.0	0.0	2.824365689831549E-5	0.0
36-37	5.648731379663098E-5	0.0	0.0	2.824365689831549E-5	0.0
38-39	5.648731379663098E-5	0.0	0.0	2.824365689831549E-5	0.0
40-41	5.648731379663098E-5	0.0	0.0	2.824365689831549E-5	0.0
42-43	5.648731379663098E-5	0.0	0.0	2.824365689831549E-5	0.0
44-45	5.648731379663098E-5	0.0	0.0	2.824365689831549E-5	0.0
46-47	5.648731379663098E-5	0.0	0.0	2.824365689831549E-5	0.0
48-49	8.473097069494647E-5	0.0	0.0	2.824365689831549E-5	0.0
50-51	8.473097069494647E-5	0.0	0.0	2.824365689831549E-5	0.0
52-53	1.1297462759326197E-4	0.0	0.0	2.824365689831549E-5	0.0
54-55	1.2709645604241972E-4	0.0	0.0	2.824365689831549E-5	0.0
56-57	1.835837698390507E-4	0.0	0.0	2.824365689831549E-5	0.0
58-59	2.5419291208483944E-4	0.0	0.0	2.824365689831549E-5	0.0
60-61	2.965583974323127E-4	0.0	0.0	2.824365689831549E-5	0.0
62-63	3.389238827797859E-4	0.0	0.0	4.2365485347473235E-5	0.0
64-65	3.671675396781014E-4	0.0	0.0	5.648731379663098E-5	0.0
66-67	4.236548534747324E-4	0.0	0.0	5.648731379663098E-5	0.0
68-69	4.660203388222056E-4	0.0	0.0	5.648731379663098E-5	0.0
70-71	4.942639957205211E-4	0.0	0.0	5.648731379663098E-5	0.0
72-73	5.366294810679943E-4	0.0	0.0	5.648731379663098E-5	0.0
74-75	6.354822802120986E-4	0.0	0.0	5.648731379663098E-5	0.0
76-77	7.76700564703676E-4	0.0	0.0	5.648731379663098E-5	0.0
78-79	8.614315353986225E-4	0.0	0.0	5.648731379663098E-5	0.0
80-81	0.001059137133686831	0.0	0.0	5.648731379663098E-5	0.0
82-83	0.0012709645604241971	0.0	0.0	5.648731379663098E-5	0.0
84-85	0.0012992082173225127	0.0	0.0	5.648731379663098E-5	0.0
86-87	0.0013274518742208282	8.473097069494647E-5	0.0	5.648731379663098E-5	0.0
88	0.0013274518742208282	8.473097069494647E-5	0.0	5.648731379663098E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	13180	0.0	79.80998	8
CACCGGC	12690	0.0	79.48872	9
CTTCACC	14685	0.0	74.09182	6
CCTTCAC	16055	0.0	71.24854	5
TTCACCG	15835	0.0	68.207054	7
GTGTGGC	5040	0.0	67.61403	7
CGTAGTG	3000	0.0	65.55767	4
GGCCCGA	1180	0.0	64.043434	9
CGGGGCA	1645	0.0	63.63114	94
CGGGCCC	1225	0.0	63.22351	7
GGGCCCG	1220	0.0	63.09788	8
AACGGGC	1240	0.0	62.83725	5
CGCTGCC	95	0.0	61.254734	1
CGCTGCA	115	0.0	59.80205	1
ACCTTCA	6425	0.0	59.175594	4
ACGGGCC	1315	0.0	58.89643	6
GCCTTCA	5105	0.0	57.37459	4
CAGCGTA	1285	0.0	57.22453	1
TGTGGCA	6065	0.0	57.193203	8
TGTGTGG	5660	0.0	55.563442	6
>>END_MODULE