FastQCFastQC Report
Tue 7 Mar 2017
1SN_22A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_22A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3060365
Sequences flagged as poor quality0
Sequence length94
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA155060.5066715898267037No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG82290.26888949520727107No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA66100.21598730870337363No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG62560.20442006100579507No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT60990.19928995397607802No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG55760.18220048915733908No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG53880.17605743105806007No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC48730.15922937296695003No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA47620.15560235462109911No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT45980.1502435167047068No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT44740.14619171242645893No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA44200.14442721701496392No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA43240.14129033628341717No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC42630.13929710998524686No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA41680.13619290509465373No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC40770.1332194035678751No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG39140.12789324149243636No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA38430.12557325678472991No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG33810.11047701826416129No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA31720.10364776750485644No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA31150.10178524457050057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCT257.290728E-787.9998863
GCTGCAT500.087.9998863
GCAGCGG257.290728E-787.9998863
GCAGCCA257.290728E-787.9998863
GCAGCAC257.290728E-787.9998863
GCAGCAA500.087.9998863
AGCAGCC302.0971129E-887.9998862
CAGCTGC4650.087.053651
AGCTGCC1850.085.6215062
AGCTGCT1300.084.615282
AGCAGCA1150.084.17382
CTGCAGC900.083.1111
CAGCCCT180500.083.051421
CAGCGTA73800.082.097461
AGCCCTT156750.080.477092
AGCTGCA1150.080.347722
AGCTGCG550.079.999912
AGCAGCG1050.079.618942
GCCCTTC141350.079.315073
GCTGCTT455.0931703E-1178.222123
Produced by FastQC (version 0.11.5)