FastQCFastQC Report
Tue 7 Mar 2017
1SN_22A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_22A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3060365
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG115450.079.89128
CACCGGC113700.077.6117869
CTTCACC130650.074.3329546
CGGGGCA13300.071.2902394
CCTTCAC145650.070.1581655
GTGTGGC48600.067.703647
TTCACCG143500.066.597237
CGTAGTG27700.066.595224
CGGGCCC9300.065.1084067
GGGCCCG9550.062.4209948
CAGCAGC1200.061.7388231
GGCCCGA9850.060.996389
TGTGGCA54250.060.912058
ACCTTCA55350.060.125534
AGCTGCT1100.059.745922
GCGTAGT17450.059.1776283
CAGCGTA10650.058.1363561
AACGGGC10200.057.9835
CGCTGCA553.8870348E-757.729811
ACGGGCC10650.056.855236
Produced by FastQC (version 0.11.5)