##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1SN_22A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3060365
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.825232937901198	12.0	12.0	12.0	2.0	32.0
2	14.674782256364846	12.0	12.0	12.0	12.0	22.0
3	13.683008399324917	12.0	12.0	12.0	12.0	22.0
4	13.813393173690066	12.0	12.0	12.0	12.0	22.0
5	22.442373703790235	22.0	22.0	22.0	22.0	27.0
6	26.860982268454908	27.0	22.0	32.0	22.0	32.0
7	34.98547689572976	37.0	37.0	37.0	27.0	41.0
8	37.603928616357855	41.0	37.0	41.0	27.0	41.0
9	38.540731252644704	41.0	41.0	41.0	32.0	41.0
10-11	38.895984302526	41.0	41.0	41.0	37.0	41.0
12-13	39.08154321461656	41.0	41.0	41.0	37.0	41.0
14-15	39.036691538427604	41.0	41.0	41.0	37.0	41.0
16-17	38.94286416816294	41.0	41.0	41.0	37.0	41.0
18-19	38.88559207806912	41.0	41.0	41.0	37.0	41.0
20-21	38.89896662652984	41.0	41.0	41.0	37.0	41.0
22-23	38.88325983992105	41.0	41.0	41.0	37.0	41.0
24-25	38.897588522937625	41.0	41.0	41.0	37.0	41.0
26-27	38.871855808049034	41.0	41.0	41.0	37.0	41.0
28-29	38.8182053447873	41.0	41.0	41.0	34.5	41.0
30-31	38.689742726766255	41.0	41.0	41.0	32.0	41.0
32-33	38.72769604279228	41.0	41.0	41.0	32.0	41.0
34-35	38.70201756979968	41.0	41.0	41.0	32.0	41.0
36-37	38.631744416107225	41.0	41.0	41.0	32.0	41.0
38-39	38.54163751709355	41.0	41.0	41.0	32.0	41.0
40-41	38.583729064997144	41.0	41.0	41.0	32.0	41.0
42-43	38.325211045087755	41.0	41.0	41.0	32.0	41.0
44-45	38.43888180004673	41.0	41.0	41.0	32.0	41.0
46-47	38.447062196829464	41.0	41.0	41.0	32.0	41.0
48-49	38.39669483868754	41.0	41.0	41.0	32.0	41.0
50-51	38.27901100032186	41.0	41.0	41.0	32.0	41.0
52-53	38.250757180924495	41.0	41.0	41.0	32.0	41.0
54-55	38.12318710349909	41.0	39.0	41.0	32.0	41.0
56-57	37.90266536834659	41.0	37.0	41.0	29.5	41.0
58-59	37.89742873807536	41.0	37.0	41.0	29.5	41.0
60-61	37.898417182264204	41.0	37.0	41.0	29.5	41.0
62-63	37.89789747301384	41.0	37.0	41.0	29.5	41.0
64-65	37.87002988205655	41.0	37.0	41.0	29.5	41.0
66-67	37.758500701713686	41.0	37.0	41.0	27.0	41.0
68-69	37.76214177067115	41.0	37.0	41.0	27.0	41.0
70-71	37.73579524011025	41.0	37.0	41.0	27.0	41.0
72-73	37.60304391796403	41.0	37.0	41.0	27.0	41.0
74-75	37.505599005347406	41.0	37.0	41.0	27.0	41.0
76-77	36.468405892761155	39.0	34.5	41.0	24.5	41.0
78-79	36.975996490614676	41.0	37.0	41.0	27.0	41.0
80-81	37.617453146928554	41.0	37.0	41.0	27.0	41.0
82-83	37.81779232215765	41.0	37.0	41.0	27.0	41.0
84-85	37.71849321894611	41.0	37.0	41.0	27.0	41.0
86-87	37.578662022340474	41.0	37.0	41.0	27.0	41.0
88-89	37.48202158892812	41.0	37.0	41.0	27.0	41.0
90-91	37.340660999586646	41.0	37.0	41.0	27.0	41.0
92-93	37.230082196077916	41.0	37.0	41.0	27.0	41.0
94-95	37.06478034482815	41.0	37.0	41.0	27.0	41.0
96-97	37.01803935151526	41.0	37.0	41.0	27.0	41.0
98-99	36.88954520130768	41.0	37.0	41.0	27.0	41.0
100	36.77034438702573	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.5114685130214731
1101	2	-0.5199432942811235
1101	3	-0.032208151417435715
1101	4	0.11875206703611596
1101	5	-1.048750080649171
1101	6	-0.7509653567628227
1101	7	-0.18499507098791668
1101	8	-0.32581782979580964
1101	9	-0.17855625187366542
1101	10-11	-0.08980298394382658
1101	12-13	-0.09996465268427812
1101	14-15	-0.009101874053953907
1101	16-17	0.029605746278413392
1101	18-19	0.05448060451818293
1101	20-21	0.08492581318771641
1101	22-23	0.005503390389598906
1101	24-25	0.0253971009756242
1101	26-27	0.08135254427642025
1101	28-29	0.046029516116611546
1101	30-31	0.004112902814057406
1101	32-33	-0.08770035571031798
1101	34-35	-0.11793613481616916
1101	36-37	-0.0194125476456648
1101	38-39	0.009366541342764378
1101	40-41	0.0279192398200081
1101	42-43	0.08987264824395425
1101	44-45	0.040233861781992175
1101	46-47	-0.006265123602354095
1101	48-49	0.11269681566646028
1101	50-51	0.056857725548944416
1101	52-53	-0.007085377218452038
1101	54-55	0.026388997758296284
1101	56-57	0.1459342997541384
1101	58-59	0.10423821078079953
1101	60-61	-0.13081646131076496
1101	62-63	-0.10191620879452756
1101	64-65	-0.1444442272640032
1101	66-67	0.01983954033948976
1101	68-69	-0.022929573419489202
1101	70-71	0.02730077286029342
1101	72-73	0.009384551229054239
1101	74-75	0.10134401876975119
1101	76-77	-0.2067401086117755
1101	78-79	0.07555956238846306
1101	80-81	-0.05742940807650854
1101	82-83	-0.07368843549254223
1101	84-85	0.0927613927314681
1101	86-87	-0.0527286272106835
1101	88-89	-0.18509816286905334
1101	90-91	-0.1553985098693076
1101	92-93	0.07880090430694509
1101	94-95	0.10148760204164375
1101	96-97	-0.11967969793644073
1101	98-99	-0.056715480133583185
1101	100	-0.18919981526193652
1102	1	1.5001187431362872
1102	2	0.8746712589790206
1102	3	0.03819529394333898
1102	4	-0.04333700441049437
1102	5	-0.7777348211205819
1102	6	-0.24615655243628964
1102	7	-0.057204181371375284
1102	8	-0.2871113275981898
1102	9	-0.19012724146952564
1102	10-11	-0.19669773757672004
1102	12-13	-0.23606810450183247
1102	14-15	-0.24509351728868012
1102	16-17	-0.344567165803916
1102	18-19	-0.12190840053526841
1102	20-21	-0.15780192937139503
1102	22-23	-0.22423886412803995
1102	24-25	-0.1617816775156058
1102	26-27	-0.24419773294293634
1102	28-29	-0.2136944796017488
1102	30-31	-0.2252007385141468
1102	32-33	-0.2931128691721696
1102	34-35	-0.020910382062979238
1102	36-37	-0.17284244756009315
1102	38-39	-0.3086901906155006
1102	40-41	-0.21691478954718946
1102	42-43	-0.1742754692498636
1102	44-45	-0.021455515776032996
1102	46-47	-0.4613081429353727
1102	48-49	-0.3445810131329097
1102	50-51	-0.21402601450264314
1102	52-53	-0.18558802490780835
1102	54-55	-0.34649609957973837
1102	56-57	-0.1517193410128428
1102	58-59	-0.2226837239980739
1102	60-61	-0.06551557662263008
1102	62-63	-0.3850557115242239
1102	64-65	-0.2040523823161351
1102	66-67	-0.1327772681629753
1102	68-69	-0.48739329679241195
1102	70-71	-0.34421327990387596
1102	72-73	-0.2861252062765516
1102	74-75	-0.3977304410059972
1102	76-77	-0.14743361060969562
1102	78-79	-0.26345950800380535
1102	80-81	-0.3514533564723834
1102	82-83	-0.29880598570764505
1102	84-85	-0.5765007005900173
1102	86-87	-0.28292639817689746
1102	88-89	-0.31487172052062107
1102	90-91	-0.46188963288353335
1102	92-93	-0.3493657591358641
1102	94-95	-0.2794625475132335
1102	96-97	-0.15923423172815632
1102	98-99	-0.2945663088493191
1102	100	-0.6303773034864761
1103	1	1.5900812101043797
1103	2	0.7230412282031491
1103	3	0.32552503899471397
1103	4	1.1045391581946955
1103	5	2.7913997298481696
1103	6	0.046730053117435943
1103	7	-0.399609868911746
1103	8	-0.16524345296667775
1103	9	-0.10674170634097635
1103	10-11	-0.26200412550863206
1103	12-13	-0.43318940443035103
1103	14-15	-0.3847715380282466
1103	16-17	-0.39333214077459644
1103	18-19	-0.3726493364556589
1103	20-21	-0.27717273377716367
1103	22-23	-0.10191159015780471
1103	24-25	-0.10148058838959173
1103	26-27	-0.28685485069479455
1103	28-29	-0.1571052382610887
1103	30-31	-0.06873469105610752
1103	32-33	-0.3346840247977383
1103	34-35	-0.4014272172655282
1103	36-37	-0.2498390479343584
1103	38-39	-0.31630859240863884
1103	40-41	-0.3223036815042235
1103	42-43	-0.3130865988468088
1103	44-45	-0.6270732246212276
1103	46-47	-0.520786056243999
1103	48-49	-0.3748957903111716
1103	50-51	-0.22638191459554236
1103	52-53	-0.3760946805837335
1103	54-55	-0.30105913912678517
1103	56-57	-0.3722703603285211
1103	58-59	-0.39147827002042135
1103	60-61	-0.2536657714630337
1103	62-63	-0.3267509085023761
1103	64-65	-0.45103507147108246
1103	66-67	-0.7078390946274027
1103	68-69	-0.4165655714738108
1103	70-71	-0.702067144162065
1103	72-73	-0.4490205845567701
1103	74-75	-0.483948573840415
1103	76-77	-0.6306355554967453
1103	78-79	-0.6981405492675492
1103	80-81	-0.3318998234195689
1103	82-83	-0.510643742669366
1103	84-85	-0.5052285479019147
1103	86-87	-0.5283212817465355
1103	88-89	-0.2627239279750526
1103	90-91	-0.45970153607930087
1103	92-93	-0.2379703532422468
1103	94-95	-0.5008577881502134
1103	96-97	-0.16622896625607808
1103	98-99	-0.4200709595525751
1103	100	-0.24108986046294234
1104	1	1.3975014707238316
1104	2	-0.7072202032806025
1104	3	0.11110591949355886
1104	4	0.08232795301136875
1104	5	-1.1533208528772079
1104	6	-0.5165628353364724
1104	7	-0.29321333813930295
1104	8	-0.2068387107012839
1104	9	0.004249357035959633
1104	10-11	-0.21295113972488622
1104	12-13	-0.061535295763405884
1104	14-15	-0.16166602946130837
1104	16-17	0.007471272038124255
1104	18-19	-0.04099519929638262
1104	20-21	-0.055759527861582114
1104	22-23	-0.018625197957874207
1104	24-25	-0.20470228872707708
1104	26-27	-0.2498821192906746
1104	28-29	-0.1768277798349729
1104	30-31	-0.09571494443850526
1104	32-33	-0.16534591614789207
1104	34-35	-0.2011813995851739
1104	36-37	-0.18964812075081028
1104	38-39	-0.21110218676031423
1104	40-41	-0.07649490500813272
1104	42-43	-0.20950482653766045
1104	44-45	-0.07421116887026358
1104	46-47	-0.38914025309474454
1104	48-49	-0.3573650933742414
1104	50-51	-0.34157531348974857
1104	52-53	-0.1596801711435134
1104	54-55	-0.2301545927462314
1104	56-57	-0.24947541828906594
1104	58-59	-0.25792775381042077
1104	60-61	-0.31957033921284506
1104	62-63	-0.1727106944109167
1104	64-65	-0.12808270924223564
1104	66-67	-0.5585776161312808
1104	68-69	-0.4339538067008917
1104	70-71	-0.4770834204124199
1104	72-73	-0.39014144479755686
1104	74-75	-0.33594937732445374
1104	76-77	-0.5310348044386117
1104	78-79	-0.38364243474828186
1104	80-81	-0.40534296623885524
1104	82-83	-0.3291705318815943
1104	84-85	-0.19857985990303462
1104	86-87	-0.18575172837824283
1104	88-89	-0.35800011136886667
1104	90-91	-0.32360658250045304
1104	92-93	-0.37748054139142795
1104	94-95	-0.3208518617430798
1104	96-97	-0.24144953259835944
1104	98-99	-0.16435147805621142
1104	100	-0.051713657551879066
1105	1	1.5709448874474035
1105	2	0.46847868140083726
1105	3	-0.047604532220804074
1105	4	-0.23072686106771734
1105	5	-1.119364679309637
1105	6	0.429568726730281
1105	7	0.08721375230702932
1105	8	-0.03186612808701028
1105	9	-0.033456273356982535
1105	10-11	0.06908374760516978
1105	12-13	-0.0398083108481444
1105	14-15	0.008697328495941292
1105	16-17	0.08127577055861934
1105	18-19	0.09659041537037183
1105	20-21	0.06495456570929292
1105	22-23	-0.013121028446008154
1105	24-25	0.06559692289375363
1105	26-27	-0.023276908952773567
1105	28-29	-0.04162385656227485
1105	30-31	0.022217060444887693
1105	32-33	0.01178694953795656
1105	34-35	-0.05792504185554037
1105	36-37	-0.18892595715696103
1105	38-39	-0.03772084713650514
1105	40-41	0.07809259266397817
1105	42-43	0.227828341256739
1105	44-45	0.1887753603990845
1105	46-47	-0.148122809429708
1105	48-49	-0.20288933448169644
1105	50-51	-0.2067997222053819
1105	52-53	-0.22485181432433876
1105	54-55	-0.06190806389072634
1105	56-57	0.18465974424947973
1105	58-59	0.034146035168824085
1105	60-61	0.06437755740809337
1105	62-63	-0.3156049357252968
1105	64-65	-0.19895923789081849
1105	66-67	-0.21969355546126934
1105	68-69	-0.3310082483173815
1105	70-71	-0.2596221668688514
1105	72-73	-0.2268103876245462
1105	74-75	-0.21019772710822338
1105	76-77	-0.2131597035633277
1105	78-79	-0.03966184418813157
1105	80-81	-0.057651199731516556
1105	82-83	-0.06303669365451725
1105	84-85	-0.13373619073259135
1105	86-87	-0.08574552066826868
1105	88-89	-0.13647694626447304
1105	90-91	-0.014684034028583426
1105	92-93	0.09076495284574548
1105	94-95	0.07866393687881157
1105	96-97	-0.02078716994393659
1105	98-99	-0.10896008194223583
1105	100	-0.02481565022678467
1106	1	1.3856424497680493
1106	2	-0.5513706529264937
1106	3	-0.06557480077062117
1106	4	-0.18054355003126865
1106	5	-1.1422164339553902
1106	6	-0.5841647931479876
1106	7	-0.13757493789561437
1106	8	-1.698286891951284E-4
1106	9	0.11221421611922722
1106	10-11	0.09280252771022646
1106	12-13	0.034846768246232784
1106	14-15	0.11682054346855608
1106	16-17	0.09507260678373797
1106	18-19	0.17803600245928664
1106	20-21	0.11711668279007625
1106	22-23	0.12416004507244338
1106	24-25	0.04863665219934887
1106	26-27	-0.012579510029148366
1106	28-29	0.049791672282403
1106	30-31	0.11894095011105321
1106	32-33	6.803772330741253E-4
1106	34-35	0.13780348760528938
1106	36-37	-0.24457830275528636
1106	38-39	0.02757566740072548
1106	40-41	0.11750531652640461
1106	42-43	0.11822229829013509
1106	44-45	0.07003031762651091
1106	46-47	-0.17418701765198108
1106	48-49	-0.14188709798492738
1106	50-51	-0.10007150932629116
1106	52-53	0.027397788246439347
1106	54-55	-0.06681405954028463
1106	56-57	-0.10629452841249787
1106	58-59	-6.115755581888038E-4
1106	60-61	0.16017621142511018
1106	62-63	-0.0901354245887731
1106	64-65	-0.3547004306210084
1106	66-67	-0.3121207842136968
1106	68-69	-0.23572089082170322
1106	70-71	-0.34155862585109276
1106	72-73	-0.09399822271610248
1106	74-75	-0.3368362077882239
1106	76-77	-0.21085750757540467
1106	78-79	-0.05575781270697178
1106	80-81	-0.08304343769205502
1106	82-83	-0.34464091202102054
1106	84-85	-0.5747822040154063
1106	86-87	-0.19942525569275915
1106	88-89	-0.17467508558085854
1106	90-91	-0.4367685034818507
1106	92-93	-0.3366711095582957
1106	94-95	-0.21759461167903993
1106	96-97	-0.14053812564632295
1106	98-99	-0.20570643869921668
1106	100	-0.28678465244966134
1107	1	1.416193176881002
1107	2	-0.5572093189724274
1107	3	-0.1441729699721055
1107	4	-0.30754796587541655
1107	5	-1.1775328568737038
1107	6	-0.3864381402366419
1107	7	-0.4776392885626777
1107	8	-0.5040012552850826
1107	9	-0.265714138668919
1107	10-11	-0.21130230657854554
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2226	100	0.6665247652454767
2227	1	1.2616032533196346
2227	2	1.674233880873734
2227	3	0.6483970987745895
2227	4	-0.1392567779517595
2227	5	3.2735452162958545
2227	6	0.11259435820093344
2227	7	0.35325826443958164
2227	8	0.44340511940843896
2227	9	0.4135560887239933
2227	10-11	0.241716779042342
2227	12-13	0.24003971494535392
2227	14-15	0.3398500830959392
2227	16-17	0.32819051594511706
2227	18-19	0.22937875591356516
2227	20-21	0.23340101559399073
2227	22-23	0.2750025437579424
2227	24-25	0.4682695005685602
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2227	28-29	0.25030696925065854
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2227	34-35	0.24490509969541563
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2227	92-93	0.21474915865360344
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2227	98-99	0.618627368034268
2227	100	0.4584739378658682
2228	1	1.3261291893293716
2228	2	1.661657094890609
2228	3	-0.23328463352991236
2228	4	-0.012109314937317706
2228	5	3.11590335439082
2228	6	-0.09123359666788744
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2228	10-11	0.15286450286937026
2228	12-13	0.15442374134043746
2228	14-15	0.22058676734095428
2228	16-17	0.14088016168528128
2228	18-19	0.12929735800049258
2228	20-21	0.18373530099930235
2228	22-23	0.195262539983581
2228	24-25	0.1369727053827745
2228	26-27	0.08860813556970726
2228	28-29	0.01579735523208825
2228	30-31	0.053162678706101474
2228	32-33	0.2073656618416635
2228	34-35	0.20748604701763185
2228	36-37	0.13997929725433522
2228	38-39	0.14931889751341032
2228	40-41	0.19786185078248764
2228	42-43	0.4712738097114908
2228	44-45	0.19687269863808154
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2228	48-49	0.19361957600595758
2228	50-51	0.14765831800666973
2228	52-53	0.04893365484949186
2228	54-55	0.12564821339901044
2228	56-57	0.32929447617582497
2228	58-59	0.16145934637075499
2228	60-61	0.09993781355714759
2228	62-63	0.24330155708599221
2228	64-65	0.2808772134141009
2228	66-67	0.3524430440521584
2228	68-69	0.3496906886560538
2228	70-71	0.3402348179068184
2228	72-73	0.4269639359493951
2228	74-75	0.3968128152710193
2228	76-77	0.26970004164586214
2228	78-79	0.4255413133323458
2228	80-81	0.10353774415298034
2228	82-83	0.11408586400099097
2228	84-85	0.22203035888530565
2228	86-87	0.24343697554662924
2228	88-89	0.22298163325078946
2228	90-91	0.3214932249528104
2228	92-93	0.42527117081213817
2228	94-95	0.6698551178729701
2228	96-97	0.5188456244856567
2228	98-99	0.4547472082388495
2228	100	0.48798021751209575
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	24.0
13	28.0
14	169.0
15	520.0
16	2025.0
17	5059.0
18	8180.0
19	10435.0
20	12362.0
21	14045.0
22	16292.0
23	18633.0
24	21482.0
25	24455.0
26	27556.0
27	31012.0
28	34966.0
29	39954.0
30	45404.0
31	52725.0
32	62180.0
33	73468.0
34	89826.0
35	116980.0
36	163285.0
37	269377.0
38	661420.0
39	1256963.0
40	1540.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.88074403488133	18.43789064888322	20.419007644103168	35.26235767213228
2	18.38662441591782	51.21440403275271	13.903435231621527	16.495536319707934
3	41.62451210884976	16.062365110044063	23.006569477823724	19.30655330328245
4	17.00901689831115	15.90771035481062	22.2848581786813	44.79841456819693
5	17.538365521759662	34.780295814388154	26.558498741163227	21.12283992268896
6	17.68756341155385	28.413538907940723	29.802588906878754	24.096308773626678
7	20.397795687769268	26.19034004113888	36.07223321401206	17.339631057079792
8	21.67470220055451	28.20029636987745	33.041189531314075	17.083811898253966
9	21.248119096905107	28.39527311284765	26.961685942689844	23.3949218475574
10-11	19.62234896817863	30.374661192374113	28.576117554605414	21.426872284841842
12-13	24.024814033620174	26.803387831190072	27.355054054009898	21.81674408117986
14-15	21.63985341617748	27.525719971310615	28.707899221171328	22.126527391340574
16-17	20.83390379905665	29.619538845856624	28.38765964190546	21.158897713181272
18-19	21.473108599791203	29.539303318395028	29.66085744674246	19.326730635071307
20-21	22.820513239433858	28.81051769968615	29.92255825693994	18.446410803940054
22-23	21.577181153228455	30.46371919689318	28.755769328168373	19.20333032170999
24-25	22.15157342343152	28.20271438210802	26.79827406208083	22.847438132379636
26-27	20.12255074149652	26.78169107279687	29.985818685026132	23.109939500680472
28-29	19.88428137982054	28.077613230754807	30.541870231046765	21.496235158377893
30-31	20.171918422407956	29.92103739277974	28.38546091966944	21.52158326514286
32-33	20.804724273085075	30.040730435748674	28.808459121706072	20.34608616946018
34-35	21.213237636687126	29.25004043635318	30.099416246101363	19.43730568085833
36-37	21.75915291149912	28.025447944934673	28.83219812015887	21.383201023407338
38-39	21.40177343590831	29.321114173779595	28.94259296504087	20.334519425271225
40-41	20.88392664731242	29.53834259628231	30.108301102126727	19.469429654278546
42-43	20.62255022973578	29.817618070533126	29.36115268660079	20.198679013130302
44-45	20.562530939936902	30.158722897432167	28.820859603347966	20.45788655928296
46-47	19.798692313687212	28.261549026146376	28.948411923639277	22.991346736527138
48-49	20.919596191957496	28.152034152788964	30.172577453996503	20.75579220125704
50-51	21.872472727926244	30.65103345515976	28.258802463104892	19.217691353809105
52-53	20.30519020717108	30.443375057714533	28.27216491341921	20.979269821695176
54-55	22.126802813221204	28.572980977470014	28.138977271631678	21.16123893767711
56-57	21.309304622278518	28.665630067392023	29.44837363063994	20.576691679689517
58-59	21.12036728231707	28.120591637483884	29.03404234250325	21.724998737695802
60-61	19.669724914536143	30.379860787528777	29.53618498227985	20.414229315655227
62-63	20.453395350752395	31.031155060268784	27.57321444882117	20.942235140157656
64-65	19.694970775520872	30.287670169791976	28.952566893627797	21.06479216105935
66-67	20.27431368480557	30.16582989283958	29.50135032912741	20.05850609322744
68-69	20.84903271341817	30.094776276685952	29.213672225371813	19.842518784524067
70-71	19.652116659287373	30.82432977765724	29.321126728347764	20.202426834707623
72-73	20.112780665051393	30.8094949458643	28.509393487378137	20.56833090170617
74-75	19.992615259944486	29.374224969897384	30.3801670715748	20.252992698583338
76-77	19.47672908296886	30.58045363869996	28.822869167566612	21.119948110764568
78-79	19.568891292378524	29.474719518750213	28.48844827332687	22.46794091554439
80-81	20.66400401521652	29.695300353749467	28.31634403556708	21.324351595466936
82-83	20.31999451045872	28.182880146649175	29.959040833364647	21.538084509527458
84-85	20.555048172358525	29.12204916733788	28.499705100535394	21.823197559768197
86-87	21.427264394933285	28.38051997065709	29.634455367251945	20.557760267157676
88-89	21.33137713965491	27.580991156283645	29.620470107323797	21.467161596737643
90-91	21.090425488462976	27.76660627082064	30.039129319541953	21.103838921174432
92-93	21.113756038903855	28.33398957313915	30.721221161528117	19.831033226428872
94-95	20.77296335567816	28.81234754677955	30.339305932462302	20.075383165079984
96-97	20.80684820274706	28.69147961109214	30.222212056405034	20.279460129755762
98-99	21.76800806439755	29.143370153560117	29.483852416296745	19.60476936574559
100	21.217403806408715	30.124413264430878	28.300219091513597	20.357963837646817
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	6.0
2	17.0
3	23.5
4	26.5
5	29.5
6	34.0
7	44.5
8	59.0
9	86.0
10	107.5
11	121.5
12	137.5
13	155.5
14	177.5
15	212.5
16	291.5
17	385.0
18	557.0
19	893.0
20	1379.0
21	2077.0
22	3153.5
23	4888.5
24	7619.5
25	11386.5
26	16418.0
27	22310.0
28	28588.5
29	36072.5
30	45251.0
31	55768.5
32	66992.0
33	79080.0
34	92507.5
35	107654.0
36	122779.0
37	133594.5
38	140546.5
39	145862.5
40	149150.5
41	149348.0
42	146860.0
43	143845.0
44	141478.0
45	137988.5
46	129669.5
47	118084.5
48	108979.5
49	103906.0
50	99240.5
51	91981.0
52	81281.0
53	68310.0
54	56444.5
55	46655.5
56	37579.0
57	28453.5
58	20146.5
59	14116.5
60	10606.5
61	8732.5
62	7040.5
63	5121.5
64	3705.0
65	3302.0
66	3703.5
67	3518.5
68	2441.0
69	2191.5
70	2830.0
71	2769.5
72	1604.5
73	579.5
74	242.5
75	155.5
76	118.0
77	88.0
78	72.0
79	77.0
80	62.0
81	41.5
82	30.5
83	26.5
84	25.5
85	18.0
86	17.5
87	16.0
88	10.5
89	13.0
90	14.5
91	13.5
92	16.0
93	19.0
94	16.0
95	12.5
96	22.5
97	23.5
98	38.0
99	89.0
100	94.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.283980832351697
2	0.007254036691701807
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	3.430963300129233E-4
30-31	0.010096834854666028
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0011273165128996051
40-41	0.00178083333197184
42-43	4.901376143041761E-5
44-45	0.0
46-47	0.002434350151044075
48-49	0.0
50-51	0.0
52-53	0.0014377370019589167
54-55	0.019556490810736626
56-57	0.011730626902346617
58-59	0.01583144494202489
60-61	2.287308866752822E-4
62-63	0.011910344027591481
64-65	8.168960238402936E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	2.287308866752822E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3060365.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.93033388642604
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.56051127443985	62.399566547382165
2	8.85722468589948	12.91920707299488
3	2.4928021950795154	5.454026891998943
4	1.0887750015849442	3.176188975711361
5	0.5928224116942094	2.16173682101075
6	0.3417071715440132	1.4952490867254682
7	0.21801603682260998	1.112998765064781
8	0.16855590688477626	0.9834270854108855
9	0.1222123207649118	0.8021686822578012
>10	0.5195277764481616	6.4350773349910835
>50	0.025743945282550088	1.268211776892478
>100	0.011553736554223815	1.5045968778127137
>500	5.019089173204037E-4	0.24811117624133097
>1k	4.5628083313773365E-5	0.0394329055054532
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	6.535168190722348E-5	0.0
3	0.0	0.0	0.0	6.535168190722348E-5	0.0
4	0.0	0.0	0.0	6.535168190722348E-5	0.0
5	0.0	0.0	0.0	6.535168190722348E-5	0.0
6	0.0	0.0	0.0	6.535168190722348E-5	0.0
7	0.0	0.0	0.0	6.535168190722348E-5	0.0
8	0.0	0.0	0.0	6.535168190722348E-5	0.0
9	3.267584095361174E-5	0.0	0.0	6.535168190722348E-5	0.0
10-11	6.535168190722348E-5	0.0	0.0	6.535168190722348E-5	0.0
12-13	6.535168190722348E-5	0.0	0.0	6.535168190722348E-5	0.0
14-15	6.535168190722348E-5	0.0	0.0	9.802752286083522E-5	0.0
16-17	6.535168190722348E-5	0.0	0.0	9.802752286083522E-5	0.0
18-19	6.535168190722348E-5	0.0	0.0	9.802752286083522E-5	0.0
20-21	6.535168190722348E-5	0.0	0.0	9.802752286083522E-5	0.0
22-23	6.535168190722348E-5	0.0	0.0	9.802752286083522E-5	0.0
24-25	6.535168190722348E-5	0.0	0.0	1.143654433376411E-4	0.0
26-27	6.535168190722348E-5	0.0	0.0	1.3070336381444696E-4	0.0
28-29	6.535168190722348E-5	0.0	0.0	1.3070336381444696E-4	0.0
30-31	6.535168190722348E-5	0.0	0.0	1.3070336381444696E-4	0.0
32-33	8.168960238402935E-5	0.0	0.0	1.3070336381444696E-4	0.0
34-35	9.802752286083522E-5	0.0	0.0	1.3070336381444696E-4	0.0
36-37	9.802752286083522E-5	0.0	0.0	1.3070336381444696E-4	0.0
38-39	1.143654433376411E-4	0.0	0.0	1.3070336381444696E-4	0.0
40-41	1.3070336381444696E-4	0.0	0.0	1.3070336381444696E-4	0.0
42-43	1.3070336381444696E-4	0.0	0.0	1.3070336381444696E-4	0.0
44-45	1.3070336381444696E-4	0.0	0.0	1.3070336381444696E-4	0.0
46-47	1.3070336381444696E-4	0.0	0.0	1.3070336381444696E-4	0.0
48-49	1.6337920476805872E-4	0.0	0.0	1.3070336381444696E-4	0.0
50-51	1.9605504572167045E-4	0.0	0.0	1.3070336381444696E-4	0.0
52-53	1.9605504572167045E-4	0.0	0.0	1.3070336381444696E-4	0.0
54-55	2.287308866752822E-4	0.0	0.0	1.3070336381444696E-4	0.0
56-57	2.287308866752822E-4	0.0	0.0	1.3070336381444696E-4	0.0
58-59	3.1042048905931155E-4	0.0	0.0	1.3070336381444696E-4	0.0
60-61	3.921100914433409E-4	0.0	0.0	1.3070336381444696E-4	0.0
62-63	4.247859323969527E-4	0.0	0.0	1.3070336381444696E-4	0.0
64-65	4.574617733505644E-4	0.0	0.0	1.3070336381444696E-4	0.0
66-67	5.554892962113995E-4	0.0	0.0	1.3070336381444696E-4	0.0
68-69	6.208409781186231E-4	0.0	0.0	1.3070336381444696E-4	0.0
70-71	6.535168190722349E-4	0.0	0.0	1.3070336381444696E-4	0.0
72-73	7.188685009794583E-4	0.0	0.0	1.3070336381444696E-4	0.0
74-75	7.5154434193307E-4	0.0	0.0	1.3070336381444696E-4	0.0
76-77	9.475993876547405E-4	0.0	0.0	1.3070336381444696E-4	0.0
78-79	0.0010783027514691874	0.0	0.0	1.3070336381444696E-4	0.0
80-81	0.0013397094790980814	0.0	0.0	1.3070336381444696E-4	0.0
82-83	0.0015684403657733638	0.0	0.0	1.3070336381444696E-4	0.0
84-85	0.0016664678886341988	0.0	0.0	1.3070336381444696E-4	0.0
86-87	0.0017318195705414224	6.535168190722348E-5	0.0	1.3070336381444696E-4	0.0
88	0.0017318195705414224	6.535168190722348E-5	0.0	1.3070336381444696E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	11545	0.0	79.8912	8
CACCGGC	11370	0.0	77.611786	9
CTTCACC	13065	0.0	74.332954	6
CGGGGCA	1330	0.0	71.29023	94
CCTTCAC	14565	0.0	70.158165	5
GTGTGGC	4860	0.0	67.70364	7
TTCACCG	14350	0.0	66.59723	7
CGTAGTG	2770	0.0	66.59522	4
CGGGCCC	930	0.0	65.108406	7
GGGCCCG	955	0.0	62.420994	8
CAGCAGC	120	0.0	61.738823	1
GGCCCGA	985	0.0	60.99638	9
TGTGGCA	5425	0.0	60.91205	8
ACCTTCA	5535	0.0	60.12553	4
AGCTGCT	110	0.0	59.74592	2
GCGTAGT	1745	0.0	59.177628	3
CAGCGTA	1065	0.0	58.136356	1
AACGGGC	1020	0.0	57.983	5
CGCTGCA	55	3.8870348E-7	57.72981	1
ACGGGCC	1065	0.0	56.85523	6
>>END_MODULE