FastQCFastQC Report
Tue 7 Mar 2017
1SN_23A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_23A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3696257
Sequences flagged as poor quality0
Sequence length94
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA172470.46660716503208516No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG97780.26453788251195737No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA83570.2260935860249977No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT78490.21234995293887846No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA76860.2079400864171512No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG66050.1786942845153895No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG65970.17847784934867894No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG61510.16641158880456636No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC52740.14268488365392343No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT49190.1330805731311432No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA48150.1302669159639062No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA47040.12726387802579744No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA44360.12001329994099437No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT43730.11830887300314887No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC43000.11633390210691519No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA37140.10048002614536813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTC500.087.999723
GCTGCCA750.087.999723
GCTGCAA950.087.999723
GCAGCCG302.0972948E-887.999723
GCAGCCC302.0972948E-887.999723
GCAGCAA1600.087.999723
AGCAGCC950.087.999722
AGCAGCA2750.087.999722
GCAGCTC356.0754246E-1087.999713
AGCAGCG1550.087.999712
CAGCAGC6450.086.635381
AGCTGCA1900.085.683942
CAGCTGC6750.085.392321
AGCTGCC1600.085.2497252
CTGCTGC3550.084.281421
GCTGCCC1050.083.809253
TGCTGCC1000.083.599732
TGCTGCA1000.083.599732
AGCTGCT1850.083.242972
GCTGCAT900.083.110853
Produced by FastQC (version 0.11.5)