FastQCFastQC Report
Tue 7 Mar 2017
1SN_23A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_23A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3696257
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG135550.079.090538
CACCGGC130850.077.2680369
CTTCACC149650.073.583286
CCTTCAC166700.069.745845
CGGGGCA16600.068.9937394
GTGTGGC56200.068.068977
TTCACCG165750.066.2942357
CGTAGTG30100.062.220614
CGGGCCC10900.061.579687
AACGGGC11350.059.5517465
TGTGGCA65650.058.3422668
ACCTTCA68650.057.7755434
GCGTAGT19850.056.9881363
ACGGGCC11700.056.967926
GCCTTCA50650.056.8079224
TGTGTGG63350.056.4593966
ATCGGAA57300.055.78537894
GGGCCCG12000.055.152578
GGCCCGA12100.053.5339
ATGTGTG62950.052.791675
Produced by FastQC (version 0.11.5)