FastQCFastQC Report
Tue 7 Mar 2017
1SN_24A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_24A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1885265
Sequences flagged as poor quality0
Sequence length93
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA122070.6474951797227445No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG63410.33634528832816607No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA45400.24081495174418452No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG42830.2271829159295908No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC40590.21530129716512003No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG40500.21482391069690465No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG39960.2119595918876126No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT36300.19254587551352198No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA35750.18962851376331707No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA32680.1733443309030826No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA30800.16337225801147318No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT28950.15355931394260225No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT28540.15138455336517678No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA26970.14305681164186468No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA26800.1421550816463468No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA24600.1304856346455273No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTTAAAAAAAAGCCATGAGTGCTC22990.12194572115856392No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA22520.11945270293566156No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC22230.1179144576491899No Hit
CAGCACTTTGAACTTTGTTCACCATTTCTTCGTAGTCGTTGATACGTTGT20810.11038236003957003No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG20380.10810151358031896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC356.6393113E-1086.999823
GCTGCCA202.6990036E-586.999823
GCTGCAG202.6990036E-586.999823
AGCTGCT356.6393113E-1086.999822
AGCTGCA500.086.999822
CAGCTGC1800.086.999821
GCAGCTC202.6990036E-586.999823
TGCAGCT257.8027006E-786.999822
AGCAGCG500.086.999822
CAGCAGC1200.086.999811
GCTGCCT159.3881256E-486.999813
GCTGCAA159.3881256E-486.999813
AGCTGCC600.086.999812
GCAGCAT159.3881256E-486.999813
GCAGCAA159.3881256E-486.999813
AGCAGCT302.269735E-886.999812
AGCAGCA302.269735E-886.999812
CAGCCCT123750.082.0083161
CAGCGTA48750.081.021371
AGCCCTT106450.080.6252
Produced by FastQC (version 0.11.5)