##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1SN_24A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1885265
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	14.03902369162956	12.0	12.0	12.0	2.0	32.0
2	14.667426064770735	12.0	12.0	12.0	12.0	22.0
3	13.784860483804664	12.0	12.0	12.0	12.0	22.0
4	13.83913932524075	12.0	12.0	12.0	12.0	22.0
5	22.427918621520053	22.0	22.0	22.0	22.0	27.0
6	26.934349282461618	27.0	22.0	32.0	22.0	32.0
7	34.924847170026496	37.0	37.0	37.0	27.0	41.0
8	37.483715021495655	41.0	37.0	41.0	27.0	41.0
9	38.39665670343427	41.0	37.0	41.0	32.0	41.0
10-11	38.7743158123659	41.0	41.0	41.0	34.5	41.0
12-13	38.94608105491801	41.0	41.0	41.0	37.0	41.0
14-15	38.913867016042836	41.0	41.0	41.0	37.0	41.0
16-17	38.852735026640815	41.0	41.0	41.0	37.0	41.0
18-19	38.77781266824558	41.0	41.0	41.0	34.5	41.0
20-21	38.8142430904939	41.0	41.0	41.0	37.0	41.0
22-23	38.80573924620677	41.0	41.0	41.0	34.5	41.0
24-25	38.819622440346585	41.0	41.0	41.0	34.5	41.0
26-27	38.780803229254246	41.0	41.0	41.0	34.5	41.0
28-29	38.71808552113363	41.0	41.0	41.0	32.0	41.0
30-31	38.61430939417005	41.0	41.0	41.0	32.0	41.0
32-33	38.653816306991324	41.0	41.0	41.0	32.0	41.0
34-35	38.61521085895086	41.0	41.0	41.0	32.0	41.0
36-37	38.54743391512599	41.0	41.0	41.0	32.0	41.0
38-39	38.46932738898775	41.0	41.0	41.0	32.0	41.0
40-41	38.52599395840903	41.0	41.0	41.0	32.0	41.0
42-43	38.28843557802219	41.0	41.0	41.0	32.0	41.0
44-45	38.39824003522051	41.0	41.0	41.0	32.0	41.0
46-47	38.39358657801423	41.0	41.0	41.0	32.0	41.0
48-49	38.35209082012343	41.0	41.0	41.0	32.0	41.0
50-51	38.238529861849656	41.0	41.0	41.0	32.0	41.0
52-53	38.22168607596279	41.0	41.0	41.0	32.0	41.0
54-55	38.08860717193604	41.0	37.0	41.0	32.0	41.0
56-57	37.885593272033375	41.0	37.0	41.0	29.5	41.0
58-59	37.887254576942766	41.0	37.0	41.0	29.5	41.0
60-61	37.92610773551729	41.0	37.0	41.0	32.0	41.0
62-63	37.92452493416045	41.0	37.0	41.0	32.0	41.0
64-65	37.90680143109854	41.0	37.0	41.0	29.5	41.0
66-67	37.766066574195136	41.0	37.0	41.0	27.0	41.0
68-69	37.754912439365285	41.0	37.0	41.0	27.0	41.0
70-71	37.70941750894437	41.0	37.0	41.0	27.0	41.0
72-73	37.59004490084948	41.0	37.0	41.0	27.0	41.0
74-75	37.4650879319353	41.0	37.0	41.0	27.0	41.0
76-77	36.379617189095434	39.0	34.5	41.0	24.5	41.0
78-79	36.90307012542003	41.0	37.0	41.0	27.0	41.0
80-81	37.58131774578109	41.0	37.0	41.0	27.0	41.0
82-83	37.77832400219597	41.0	37.0	41.0	27.0	41.0
84-85	37.69801698965398	41.0	37.0	41.0	27.0	41.0
86-87	37.57370634897481	41.0	37.0	41.0	27.0	41.0
88-89	37.48350894967021	41.0	37.0	41.0	27.0	41.0
90-91	37.331417599117366	41.0	37.0	41.0	27.0	41.0
92-93	37.24385139489674	41.0	37.0	41.0	27.0	41.0
94-95	37.10635640082429	41.0	37.0	41.0	27.0	41.0
96-97	37.04628739195816	41.0	37.0	41.0	27.0	41.0
98-99	36.918437461046594	41.0	37.0	41.0	27.0	41.0
100	36.79216290548013	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.449389971686042
1101	2	-0.457852276533373
1101	3	0.010658883177681489
1101	4	0.012141793440324733
1101	5	-1.1126167365702315
1101	6	-1.018859959383871
1101	7	-0.3552935650597533
1101	8	-0.26105883153327625
1101	9	-0.005940240317983125
1101	10-11	-0.0611485709108166
1101	12-13	-0.08252984405979902
1101	14-15	-0.06292010622609467
1101	16-17	-0.2515620562775638
1101	18-19	-0.033923907132816566
1101	20-21	-0.2009366145889686
1101	22-23	-0.007971235615428895
1101	24-25	0.01065161618493704
1101	26-27	-0.05311103993534516
1101	28-29	-0.2301020739726951
1101	30-31	-0.277735171950539
1101	32-33	-0.37550920418027545
1101	34-35	-0.13438913712809608
1101	36-37	-0.1416920642750057
1101	38-39	0.021060650407719095
1101	40-41	-0.040459382523415854
1101	42-43	-0.16913838785784918
1101	44-45	-0.27748998739788533
1101	46-47	-0.2732721035146355
1101	48-49	-0.30365125290805395
1101	50-51	-0.29263776646983786
1101	52-53	-0.16028138498491984
1101	54-55	-0.08284230221466515
1101	56-57	-0.1085793281376155
1101	58-59	-0.22321399680788545
1101	60-61	-0.29334237730254387
1101	62-63	-0.4970883432721678
1101	64-65	-0.23221559943802816
1101	66-67	-0.30748950287489407
1101	68-69	-0.35845719326692915
1101	70-71	-0.17978605674360182
1101	72-73	-0.09721918073453395
1101	74-75	-0.23584599818068597
1101	76-77	-0.5105717060665711
1101	78-79	-0.27195222991043266
1101	80-81	-0.06796741231801917
1101	82-83	-0.08080651280318563
1101	84-85	-0.20294627675726673
1101	86-87	-0.2905476316881135
1101	88-89	-0.3862855878157134
1101	90-91	-0.3656903850622939
1101	92-93	-0.10503365314181679
1101	94-95	-0.10239572982595746
1101	96-97	-0.47121336912046274
1101	98-99	-0.41456462939851946
1101	100	-0.5225924227083638
1102	1	1.651440635135982
1102	2	0.8509535135751918
1102	3	-0.0044195245424631935
1102	4	0.07848678740896275
1102	5	-0.9256444808404396
1102	6	-0.17627853598218124
1102	7	-0.245522756857099
1102	8	-0.504725900290822
1102	9	-0.5083527855532068
1102	10-11	-0.49962866741262246
1102	12-13	-0.5472463699946601
1102	14-15	-0.35694905676891864
1102	16-17	-0.3875692939132662
1102	18-19	-0.21335695900253882
1102	20-21	-0.33362660252623755
1102	22-23	-0.2624947579314636
1102	24-25	-0.1464653922589676
1102	26-27	-0.3622183981983156
1102	28-29	-0.42732764695218606
1102	30-31	-0.18636002116646466
1102	32-33	-0.43310872167123193
1102	34-35	-0.29391869080723154
1102	36-37	-0.36002740806269884
1102	38-39	-0.33961486225814497
1102	40-41	-0.3999286225716645
1102	42-43	-0.23789592706170737
1102	44-45	-0.24522219487677432
1102	46-47	-0.6432962289669888
1102	48-49	-0.5982833397596892
1102	50-51	-0.5709851729837041
1102	52-53	-0.29478078184860834
1102	54-55	-0.5671607581857145
1102	56-57	-0.10224639689515413
1102	58-59	-0.2690523562771219
1102	60-61	-0.28761258236888665
1102	62-63	-0.49135854833851056
1102	64-65	-0.6574266971461142
1102	66-67	-0.4501312399074635
1102	68-69	-0.5149710654020367
1102	70-71	-0.6930550555373358
1102	72-73	-0.5233349829058227
1102	74-75	-0.5736023311119354
1102	76-77	-0.15894323803279775
1102	78-79	-0.38715126489233853
1102	80-81	-0.3906453375291221
1102	82-83	-0.4016750290878619
1102	84-85	-0.6592188943688413
1102	86-87	-0.40665137113322913
1102	88-89	-0.45986821749001905
1102	90-91	-0.5237120979694083
1102	92-93	-0.4608840753372334
1102	94-95	-0.34877691197311833
1102	96-97	-0.31439792883095663
1102	98-99	-0.556301661967872
1102	100	-0.7234368135406939
1103	1	1.27804036286728
1103	2	0.7464372229305898
1103	3	0.302460553470123
1103	4	0.9757172809071673
1103	5	2.7426730447052563
1103	6	-0.1170794771432142
1103	7	-0.7908578814580522
1103	8	-0.327086228385042
1103	9	-0.3184960621117128
1103	10-11	-0.3692009997832173
1103	12-13	-0.5332240099647834
1103	14-15	-0.5642982242320684
1103	16-17	-0.5544427217702577
1103	18-19	-0.4912521996680965
1103	20-21	-0.24783735878013857
1103	22-23	-0.2633830606656389
1103	24-25	-0.32799861097900873
1103	26-27	-0.3401647824371494
1103	28-29	-0.2571809558519149
1103	30-31	-0.2051713133095916
1103	32-33	-0.4277177715488918
1103	34-35	-0.4786528560561436
1103	36-37	-0.4490276630350323
1103	38-39	-0.40275219989966615
1103	40-41	-0.4746821120395168
1103	42-43	-0.2300156417909065
1103	44-45	-0.6303869800663122
1103	46-47	-0.754931615548287
1103	48-49	-0.7378087482745741
1103	50-51	-0.4483233512883942
1103	52-53	-0.415517636981086
1103	54-55	-0.19754555765966586
1103	56-57	-0.3132076724128936
1103	58-59	-0.6433953551990825
1103	60-61	-0.5661650104957658
1103	62-63	-0.41729132212437037
1103	64-65	-0.5055653245599601
1103	66-67	-0.6098710339315687
1103	68-69	-0.5797873436707874
1103	70-71	-0.5910318277068782
1103	72-73	-0.32250065677086326
1103	74-75	-0.393075954895032
1103	76-77	-0.4794270592181604
1103	78-79	-0.6926744725933958
1103	80-81	-0.5153398553257347
1103	82-83	-0.4568780303060649
1103	84-85	-0.51419592449286
1103	86-87	-0.5234454742643138
1103	88-89	-0.41764643927420764
1103	90-91	-0.7540665542446305
1103	92-93	-0.6454739799239064
1103	94-95	-0.5517673199437638
1103	96-97	-0.3015646941565038
1103	98-99	-0.5545407126954416
1103	100	-0.7022047752978224
1104	1	1.5088009000646068
1104	2	-0.5892864888126024
1104	3	0.035887234151324776
1104	4	0.06153927591952524
1104	5	-1.0255186848788682
1104	6	-0.4105756983659852
1104	7	-0.12863006270508492
1104	8	-0.005639886574108743
1104	9	0.07517449535397702
1104	10-11	0.023478332681285963
1104	12-13	-0.030707622581743976
1104	14-15	-0.2229341345141691
1104	16-17	-0.06410823788237963
1104	18-19	0.021455435394003075
1104	20-21	0.10722745595869299
1104	22-23	-0.0304177705117894
1104	24-25	-0.0682098211649631
1104	26-27	0.05686251233731099
1104	28-29	0.024872071608463386
1104	30-31	0.05651208700059129
1104	32-33	0.0033686856506989216
1104	34-35	0.07712989278702764
1104	36-37	-0.23588743239199061
1104	38-39	-0.08183960897365239
1104	40-41	-0.13490827172614672
1104	42-43	0.02225419818826424
1104	44-45	-0.05803445532392004
1104	46-47	-0.15279571682891202
1104	48-49	-0.30119903244190027
1104	50-51	-0.013841738652033087
1104	52-53	-0.08751391750950432
1104	54-55	-0.3088469773400391
1104	56-57	-0.29911325482797935
1104	58-59	-0.03964962501967051
1104	60-61	0.01995836167593268
1104	62-63	-0.0921756760993091
1104	64-65	-0.10053605591430426
1104	66-67	-0.5962682191882536
1104	68-69	-0.5232597307345515
1104	70-71	-0.3246019236328408
1104	72-73	-0.22424853239758136
1104	74-75	-0.28398115312884187
1104	76-77	-0.19712282195974495
1104	78-79	-0.13548162925656015
1104	80-81	-0.30875420490683325
1104	82-83	-0.1268457963777081
1104	84-85	-0.13987448934079083
1104	86-87	-0.2550592295952967
1104	88-89	-0.31832755911414523
1104	90-91	-0.3343218371102239
1104	92-93	-0.28357819823435904
1104	94-95	-0.29278624329624137
1104	96-97	-0.2496836232530555
1104	98-99	-0.24980122117241876
1104	100	-0.34861253113458446
1105	1	1.5824047253280575
1105	2	0.4884007837588449
1105	3	0.062310905647184
1105	4	-0.18859910062895402
1105	5	-1.2565735962643103
1105	6	0.3842970487745312
1105	7	-0.08523767680770789
1105	8	-0.35415595432326796
1105	9	-0.149151923318108
1105	10-11	-0.27638936148332505
1105	12-13	-0.21171414681281675
1105	14-15	-0.12026694886105105
1105	16-17	-0.09774623768954172
1105	18-19	-0.07885542082651398
1105	20-21	-0.164600328623834
1105	22-23	-0.09571971706809279
1105	24-25	-0.2177218429623906
1105	26-27	-0.32159484804520844
1105	28-29	-0.07906603568603288
1105	30-31	-0.10118289894749921
1105	32-33	-0.1465868553033971
1105	34-35	-0.2592915289739821
1105	36-37	-0.25375407668034455
1105	38-39	-0.24658233572371557
1105	40-41	-0.20817660081593203
1105	42-43	-0.0770676674489934
1105	44-45	-0.12092793573516047
1105	46-47	-0.2795679186765554
1105	48-49	-0.31727642332083406
1105	50-51	-0.3032179907366128
1105	52-53	-0.29514658937318217
1105	54-55	-0.07061041236377719
1105	56-57	0.008208203714410445
1105	58-59	-0.14618278361169246
1105	60-61	-0.3051520717372753
1105	62-63	-0.3598630558009859
1105	64-65	-0.44030797565233115
1105	66-67	-0.3236417787330197
1105	68-69	-0.347812372744599
1105	70-71	-0.5198946212791853
1105	72-73	-0.4146130608638714
1105	74-75	-0.5205688052984101
1105	76-77	-0.41194071842193836
1105	78-79	-0.27208070865076905
1105	80-81	-0.45348286188654185
1105	82-83	-0.4144236889832271
1105	84-85	-0.20727672407740982
1105	86-87	-0.26524410066407
1105	88-89	-0.3223249451365078
1105	90-91	-0.40225008128144424
1105	92-93	-0.3090892987117968
1105	94-95	-0.3738356196197401
1105	96-97	-0.3412685436017284
1105	98-99	-0.5097805807479006
1105	100	-0.528574535612627
1106	1	1.2752851028421457
1106	2	-0.6165680093026769
1106	3	-0.1786626944458547
1106	4	-0.32668143337844135
1106	5	-1.1279537852328083
1106	6	-0.4854873748926991
1106	7	-0.09395341701655013
1106	8	-0.20734413691997844
1106	9	-0.4178219896791049
1106	10-11	-0.34391352879903536
1106	12-13	-0.13747793266731634
1106	14-15	-0.28656028351009155
1106	16-17	-0.3142961353110465
1106	18-19	-0.2218542120202045
1106	20-21	-0.23916482355072333
1106	22-23	-0.086546533537458
1106	24-25	-0.3921825497087994
1106	26-27	-0.26648191452251524
1106	28-29	-0.4512666622917507
1106	30-31	-0.2121019794088852
1106	32-33	-0.3274531782414698
1106	34-35	-0.3054598244161113
1106	36-37	-0.5706877614955488
1106	38-39	-0.457724146677684
1106	40-41	-0.33406391046418094
1106	42-43	-0.18604596179861943
1106	44-45	-0.2205478489689412
1106	46-47	-0.30288307879933285
1106	48-49	-0.36191802810812845
1106	50-51	-0.5512830195823284
1106	52-53	-0.5846944676185402
1106	54-55	-0.3634946665161749
1106	56-57	-0.3611052083969142
1106	58-59	-0.4930111648332556
1106	60-61	-0.3007431004913528
1106	62-63	-0.30604967716972453
1106	64-65	-0.5940697245096018
1106	66-67	-0.39228224167412407
1106	68-69	-0.3735650573166396
1106	70-71	-0.5781435789921119
1106	72-73	-0.37164422551174425
1106	74-75	-0.5116298059644819
1106	76-77	-0.28816600044738294
1106	78-79	-0.44125984810754915
1106	80-81	-0.5101008121316539
1106	82-83	-0.43754924624997926
1106	84-85	-0.49103977689484424
1106	86-87	-0.36076200192256636
1106	88-89	-0.43407327377676097
1106	90-91	-0.590247480409559
1106	92-93	-0.5372465563073661
1106	94-95	-0.4519974586969653
1106	96-97	-0.5933518842771832
1106	98-99	-0.8256857229324268
1106	100	-0.8173978349137769
1107	1	1.6771683953929326
1107	2	-0.6894348190072481
1107	3	-0.012598198223921386
1107	4	-0.27924571073802795
1107	5	-1.2624016421757673
1107	6	-0.5549166105966812
1107	7	-0.8608133522907053
1107	8	-0.6709735204457417
1107	9	-0.5431777306896066
1107	10-11	-0.3602169796482926
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2222	5	3.246426531842719
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2223	2	-0.918001922018945
2223	3	-0.013057663202479475
2223	4	-0.09197060212121144
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2223	8	0.21810705668451646
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2223	12-13	-0.09338858890176738
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2223	50-51	0.031940866501102505
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2223	66-67	0.18598545057680127
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2223	94-95	0.518752887049601
2223	96-97	0.16030982677581562
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2224	2	-0.8661368766439708
2224	3	-0.0023386326456940054
2224	4	-0.44696885986660995
2224	5	-1.0338508594725084
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2224	7	0.23252293823959036
2224	8	0.3298206300278821
2224	9	0.4105769555136405
2224	10-11	0.2934260962181128
2224	12-13	0.19888052071589613
2224	14-15	0.11090671485874992
2224	16-17	0.2752280838047838
2224	18-19	0.15113973019458626
2224	20-21	0.14239801288482568
2224	22-23	0.31717224205993944
2224	24-25	0.3176637562730136
2224	26-27	0.3626368347612896
2224	28-29	0.21271494507265487
2224	30-31	0.07675389566662716
2224	32-33	0.39122639576126517
2224	34-35	0.24585857854685855
2224	36-37	0.2532751858532194
2224	38-39	0.37269305816798237
2224	40-41	0.25529301927711145
2224	42-43	0.39049617743989273
2224	44-45	0.45939645392597583
2224	46-47	0.3105380561087543
2224	48-49	0.2093258479689979
2224	50-51	0.154458153692687
2224	52-53	0.07756420065900471
2224	54-55	0.30922029502494297
2224	56-57	0.5565528747568678
2224	58-59	0.3923915463156433
2224	60-61	0.3681051068597583
2224	62-63	0.44652951472003366
2224	64-65	0.31646724255171677
2224	66-67	0.31493606494714044
2224	68-69	0.39452553783742417
2224	70-71	0.24269445235501763
2224	72-73	0.20514336579005032
2224	74-75	0.22452142787422957
2224	76-77	0.3967888491053557
2224	78-79	0.29994419682927287
2224	80-81	0.24264814487391817
2224	82-83	0.06584694025963245
2224	84-85	0.1122207389930523
2224	86-87	0.28878055182577356
2224	88-89	0.2296480270767276
2224	90-91	0.27093682225901006
2224	92-93	0.18656735141679803
2224	94-95	0.3838893659738716
2224	96-97	0.18499535329071648
2224	98-99	0.23725416324007398
2224	100	0.4154113250459872
2225	1	1.3053635846505536
2225	2	1.995107363892
2225	3	0.9286508593997258
2225	4	1.0914460290665264
2225	5	3.252452342831557
2225	6	0.16450914437124098
2225	7	0.09988279712395354
2225	8	0.23997369225393328
2225	9	0.2150419247918265
2225	10-11	0.19790452644691925
2225	12-13	0.1560775020977161
2225	14-15	0.2615526301613258
2225	16-17	0.23250218521621946
2225	18-19	0.1287156794019353
2225	20-21	0.3226152529973376
2225	22-23	0.19302091293127432
2225	24-25	0.08812650449434756
2225	26-27	0.04096077814677557
2225	28-29	0.07643999674308333
2225	30-31	0.1366582808517265
2225	32-33	0.21520869180683633
2225	34-35	0.23601631004014934
2225	36-37	0.2983564002553081
2225	38-39	0.32785301913122566
2225	40-41	0.18301472534042773
2225	42-43	0.33561335781944024
2225	44-45	0.347680379027004
2225	46-47	0.232538157065882
2225	48-49	0.26584115839814615
2225	50-51	0.25739726813039
2225	52-53	0.2392316198302069
2225	54-55	0.1645785245734146
2225	56-57	0.3413792284938211
2225	58-59	0.30395134799096724
2225	60-61	0.28752203737040105
2225	62-63	0.31409794822994286
2225	64-65	0.27903107690350026
2225	66-67	0.3065766042825615
2225	68-69	0.4513426680188388
2225	70-71	0.48760242712695145
2225	72-73	0.5754814599998852
2225	74-75	0.375640579722166
2225	76-77	0.6801881317488636
2225	78-79	0.6555895232152693
2225	80-81	0.49219418833595796
2225	82-83	0.3146098648755
2225	84-85	0.3452483732029279
2225	86-87	0.44147470500578834
2225	88-89	0.5326445608819483
2225	90-91	0.5252797315630531
2225	92-93	0.5059694187944643
2225	94-95	0.5567984316436778
2225	96-97	0.47879111701738
2225	98-99	0.6108386601510674
2225	100	0.764746129736011
2226	1	1.7262725411551312
2226	2	1.5227656906406892
2226	3	1.0203430334493273
2226	4	1.5307949311584963
2226	5	3.0710044808183063
2226	6	-0.4282052113748698
2226	7	0.004565238164587981
2226	8	0.15804176177360318
2226	9	0.14596012691216487
2226	10-11	0.11890261436586513
2226	12-13	0.12444221652702936
2226	14-15	0.08391973416141241
2226	16-17	0.10450699831260124
2226	18-19	-0.024876139749011372
2226	20-21	0.11248069285733209
2226	22-23	0.20151527819889026
2226	24-25	0.021980207303336385
2226	26-27	0.040964491955705284
2226	28-29	0.04736122650194119
2226	30-31	0.25023080234407047
2226	32-33	0.10333603371705635
2226	34-35	0.02746984533866481
2226	36-37	0.04878065871377402
2226	38-39	-0.0019692910277058218
2226	40-41	0.1539803439584304
2226	42-43	0.32456283091837435
2226	44-45	0.2281924672982285
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2226	50-51	0.3394082496865565
2226	52-53	0.3691777943594161
2226	54-55	0.2891692097746983
2226	56-57	0.2041000498044383
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2226	60-61	0.25786638880850177
2226	62-63	0.35381448203657584
2226	64-65	0.3749605300964234
2226	66-67	0.20303755658805756
2226	68-69	0.36973684533234064
2226	70-71	0.49370633737868985
2226	72-73	0.26099771127407223
2226	74-75	0.4833627910584042
2226	76-77	0.5513019843976608
2226	78-79	0.3607921450936331
2226	80-81	0.43581980668514575
2226	82-83	0.37345233783366893
2226	84-85	0.27871371770645936
2226	86-87	0.32667998667873377
2226	88-89	0.3568713989167307
2226	90-91	0.34236562241385116
2226	92-93	0.3253626460802792
2226	94-95	0.44778275371084675
2226	96-97	0.46058850150554065
2226	98-99	0.6174050280388528
2226	100	0.6337098638612417
2227	1	1.5297767893767862
2227	2	1.7797472481937682
2227	3	0.7567714218279722
2227	4	-0.08483760440748789
2227	5	3.3871473636857097
2227	6	0.4272449089066441
2227	7	0.5647827333114535
2227	8	0.5883336542638915
2227	9	0.5065588280227473
2227	10-11	0.4874171079920018
2227	12-13	0.3986790408218468
2227	14-15	0.40935896027664853
2227	16-17	0.38941047298015974
2227	18-19	0.2085043261590016
2227	20-21	0.3406645947228526
2227	22-23	0.49187589990319225
2227	24-25	0.574875140599481
2227	26-27	0.474462094412452
2227	28-29	0.4427057046404599
2227	30-31	0.34246316473917204
2227	32-33	0.29953220490823185
2227	34-35	0.42476905535885123
2227	36-37	0.29786133988131525
2227	38-39	0.3774203193665713
2227	40-41	0.44515326575513114
2227	42-43	0.34301659972123844
2227	44-45	0.34144201630994075
2227	46-47	0.5151715949591775
2227	48-49	0.39756652524921776
2227	50-51	0.36891882450389346
2227	52-53	0.44608859554249847
2227	54-55	0.5397778058505764
2227	56-57	0.6460292193929291
2227	58-59	0.6145211702720914
2227	60-61	0.4614543111281719
2227	62-63	0.49183630846817294
2227	64-65	0.4203116755653511
2227	66-67	0.5137804409650499
2227	68-69	0.6086695261418953
2227	70-71	0.5845444908983737
2227	72-73	0.6225700942210892
2227	74-75	0.5813942932061522
2227	76-77	0.6208240895632073
2227	78-79	0.5842648861355997
2227	80-81	0.5648180933070392
2227	82-83	0.5311214031817784
2227	84-85	0.5303533238774989
2227	86-87	0.47745564341078506
2227	88-89	0.47546721240831147
2227	90-91	0.6149339966958536
2227	92-93	0.5465169173231033
2227	94-95	0.6680264221730852
2227	96-97	0.749738580417258
2227	98-99	0.9578908553790768
2227	100	0.825600664850235
2228	1	1.4392525314598732
2228	2	1.6066804295765849
2228	3	-0.11312440807525981
2228	4	-0.11812629273187447
2228	5	3.1488471716954685
2228	6	0.27607657455442336
2228	7	0.19633792585289456
2228	8	0.29733886992720215
2228	9	0.15496184431686544
2228	10-11	0.10795523187081812
2228	12-13	0.2870109806391312
2228	14-15	0.2491791823897458
2228	16-17	0.23441790347496294
2228	18-19	0.0408110151791945
2228	20-21	0.22817939486544958
2228	22-23	0.27543589434821314
2228	24-25	0.38721559613605194
2228	26-27	0.17984172254409714
2228	28-29	0.35172437823270997
2228	30-31	0.2934111612973851
2228	32-33	0.23761016210914931
2228	34-35	0.09546373503867756
2228	36-37	0.3224911394583998
2228	38-39	0.25710070787121
2228	40-41	0.21612329609273928
2228	42-43	0.4284300659540037
2228	44-45	0.4155406214099244
2228	46-47	0.264419597387338
2228	48-49	0.3123153773880958
2228	50-51	0.24622242106381975
2228	52-53	0.22375054525279836
2228	54-55	0.2219401800583114
2228	56-57	0.49068373717671676
2228	58-59	0.48144533913760057
2228	60-61	0.3394095076783614
2228	62-63	0.4331472109654726
2228	64-65	0.22543710632645997
2228	66-67	0.4500367457900012
2228	68-69	0.42549297529454577
2228	70-71	0.35379272955613317
2228	72-73	0.3552136609731349
2228	74-75	0.2568026482893089
2228	76-77	0.5154268515883658
2228	78-79	0.4721574746823265
2228	80-81	0.29552117703396164
2228	82-83	0.28385200264660426
2228	84-85	0.2131162939565101
2228	86-87	0.20260446806113208
2228	88-89	0.3930007104819708
2228	90-91	0.4221387848551643
2228	92-93	0.33725131221022764
2228	94-95	0.5280263792222399
2228	96-97	0.48502398157091875
2228	98-99	0.7070144793416304
2228	100	0.6706508672202673
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	12.0
13	20.0
14	95.0
15	319.0
16	1302.0
17	3148.0
18	5298.0
19	6760.0
20	7911.0
21	9095.0
22	10414.0
23	11942.0
24	13568.0
25	15183.0
26	17377.0
27	19335.0
28	21733.0
29	24886.0
30	28562.0
31	32824.0
32	38355.0
33	46279.0
34	56113.0
35	72343.0
36	100623.0
37	165467.0
38	400013.0
39	775313.0
40	975.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.1324490165633	21.274781725900464	21.405659491806837	33.187109765729396
2	18.597139513689743	53.789491887579345	11.157509227460753	16.455859371270158
3	39.54117856110414	17.01707717482688	24.94195776190615	18.499786502162827
4	16.764380604318227	18.650640626118875	22.29203851978369	42.29294024977921
5	16.85153015623798	34.230625402794836	27.067547533105422	21.85029690786176
6	16.991510477306903	28.194497855739115	29.848695011046196	24.96529665590779
7	20.0757453196235	26.311579539215973	36.59013454341963	17.0225405977409
8	21.492681400227553	28.10199096678716	33.551940973815356	16.85338665916993
9	20.725892646391884	28.4559995544393	27.02055148745667	23.797556311712146
10-11	19.2858563650203	30.523586869750407	28.6944275738424	21.49612919138689
12-13	24.294833882769794	26.58464459903515	27.376230927747557	21.744290590447495
14-15	21.465868193596126	27.579226262620903	28.749194410334887	22.205711133448084
16-17	20.666033687571776	30.294759622652517	28.179062359933482	20.86014432984222
18-19	21.334242135720974	29.89659278669047	29.80283938862706	18.9663256889615
20-21	22.56552792313017	29.31227174959489	29.985492755660342	18.1367075716146
22-23	21.84029831349969	30.87862979475034	28.563278902435467	18.7177929893145
24-25	22.290102452440372	28.30808931370391	26.602970935120528	22.79883729873519
26-27	19.85914447040602	26.58342461139415	30.280835850662903	23.276595067536924
28-29	19.186694440068383	28.418990089945297	30.89631811471858	21.497997355267742
30-31	19.80336575735641	30.524910567147646	28.299692293165222	21.372031382330718
32-33	20.25487133108608	30.431186066680283	29.116569819097048	20.19737278313659
34-35	21.199274372568315	29.530516929980667	30.103990685659575	19.166218011791447
36-37	21.605689385842307	28.398554049430718	28.86848798444781	21.127268580279164
38-39	21.50622332906333	29.33417142069068	29.118072186904087	20.041533063341898
40-41	20.657026842807397	30.135818611472843	30.128180306433944	19.078974239285817
42-43	20.336144349205668	30.278478608935682	29.599899006053004	19.785478035805642
44-45	20.35811941557288	30.462110101232454	28.996772337045456	20.182998146149213
46-47	19.391068739421	28.381579666256634	29.181220663253804	23.04613093106856
48-49	20.491177632852676	28.32625652096655	30.539738445258358	20.642827400922418
50-51	21.518725484215746	31.376305187864833	28.117187769358686	18.98778155856073
52-53	20.17434398883749	30.68274915879344	28.340923442842907	20.801983409526166
54-55	22.303407297372573	28.738252185100205	28.220857593803466	20.737482923723753
56-57	21.36962203804606	28.854750929825556	29.624287345937546	20.151339686190838
58-59	21.151531323822827	28.238787948878596	28.98952945963481	21.620151267663765
60-61	19.245759005590475	30.685802140235868	30.045015023130766	20.023423831042887
62-63	20.284905740952002	31.67514082558794	27.43036039732749	20.609593036132566
64-65	19.453880199271826	30.550257152313947	29.1813242071137	20.814538441300527
66-67	20.060283302347415	30.758911877110112	29.72632494636033	19.454479874182145
68-69	20.575489387433596	30.228455946511502	29.582949877072984	19.613104788981918
70-71	19.389210535388923	31.397628450111785	29.34375273502664	19.869408279472648
72-73	19.604909654610893	31.42929508583674	28.749459625039453	20.21633563451292
74-75	19.553325394573175	29.40278952826261	30.809276149506832	20.234608927657387
76-77	18.979055994780573	30.90899687842293	28.860266328606322	21.251680798190176
78-79	19.036581064200522	29.826019153805966	28.790780076010535	22.346619705982977
80-81	20.731124813321454	29.797542460335038	28.333261283128397	21.13807144321511
82-83	19.78796084370102	28.491458760439514	30.1469289463285	21.573651449530967
84-85	20.438373385173968	29.42222976610715	28.41908166756398	21.720315181154902
86-87	20.819142136516618	30.20970526689882	29.085804913367618	19.88534768321695
88-89	20.379681371053938	30.94684301676422	28.594441630221745	20.079033981960094
90-91	19.668932484292657	32.32465992844507	28.384073326561516	19.62233426070075
92-93	19.619496463361916	32.645331027733505	29.08527448395849	18.649898024946097
94-95	19.887655051146655	32.09129750989914	28.984148117108205	19.036899321846
96-97	19.30893004431738	32.26917701225027	29.139457848100932	19.282435095331426
98-99	20.993414718885674	32.30834922411438	27.967500590102716	18.730735466897226
100	19.67659718925456	34.39972629842489	27.614048953330165	18.30962755899038
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	4.5
2	7.0
3	7.0
4	9.5
5	19.5
6	27.5
7	31.0
8	47.5
9	47.0
10	49.5
11	72.5
12	89.0
13	104.5
14	116.0
15	141.5
16	184.0
17	259.5
18	385.5
19	612.0
20	978.0
21	1509.0
22	2270.5
23	3394.0
24	5170.5
25	7840.0
26	11182.0
27	15138.5
28	19855.5
29	24959.5
30	30830.0
31	37915.0
32	45489.5
33	53471.0
34	63157.5
35	74394.0
36	84666.5
37	90931.0
38	93434.0
39	94949.0
40	95398.0
41	94143.0
42	91782.5
43	89321.0
44	87516.5
45	84100.0
46	76450.5
47	67337.5
48	61119.0
49	57952.5
50	55449.5
51	50662.5
52	43292.5
53	35786.5
54	29542.0
55	24106.5
56	18543.5
57	13448.5
58	9553.0
59	6964.0
60	5515.5
61	4502.0
62	3523.0
63	2649.5
64	2003.5
65	1648.0
66	1616.5
67	1507.5
68	1149.5
69	1065.0
70	1224.5
71	1052.0
72	603.5
73	266.5
74	115.5
75	66.0
76	51.0
77	39.0
78	30.0
79	29.5
80	31.5
81	23.5
82	21.0
83	19.5
84	14.5
85	9.0
86	7.5
87	10.5
88	8.5
89	5.5
90	5.0
91	5.5
92	8.0
93	8.0
94	7.0
95	11.0
96	10.5
97	11.0
98	31.5
99	65.5
100	58.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.282870047446911
2	0.006948625259578892
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	4.2434352730252775E-4
30-31	0.01015772318480426
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0010608588182563195
40-41	0.0015912882273844792
42-43	1.856502931948559E-4
44-45	0.0
46-47	0.002333889400163903
48-49	0.0
50-51	0.0
52-53	0.0014056379341896232
54-55	0.01803459991035743
56-57	0.012412048173598937
58-59	0.013897250519157784
60-61	2.9173617502048786E-4
62-63	0.011695968471275922
64-65	5.304294091281597E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	2.9173617502048786E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1885265.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.30466922729293
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.69748996976134	67.79425454176238
2	7.8388088090057275	12.119530442323558
3	2.0836754938443187	4.832335344859538
4	0.8788262711917594	2.7174949681093676
5	0.4378973696556927	1.6925755658367472
6	0.26712039132058096	1.2389792096941534
7	0.18276667036844488	0.9890101899024416
8	0.1163154100472043	0.7193379439788858
9	0.08462986461491924	0.5888055321726222
>10	0.3871491509936141	5.097257961508332
>50	0.01687754406925466	0.877300983467427
>100	0.008094376329052183	1.153115170995202
>500	3.486787981793205E-4	0.18000214538929382
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	1.0608588182563194E-4	0.0	0.0	0.0	0.0
56-57	2.386932341076719E-4	0.0	0.0	0.0	0.0
58-59	3.447791159333038E-4	0.0	0.0	0.0	0.0
60-61	4.2434352730252775E-4	0.0	0.0	0.0	0.0
62-63	4.773864682153437E-4	0.0	0.0	0.0	0.0
64-65	5.039079386717517E-4	0.0	0.0	0.0	0.0
66-67	5.304294091281597E-4	0.0	5.304294091281597E-5	0.0	0.0
68-69	6.099938204973837E-4	0.0	1.0608588182563194E-4	0.0	0.0
70-71	6.630367614101995E-4	0.0	1.0608588182563194E-4	0.0	0.0
72-73	6.895582318666076E-4	0.0	1.0608588182563194E-4	0.0	0.0
74-75	6.895582318666076E-4	0.0	1.0608588182563194E-4	0.0	0.0
76-77	6.895582318666076E-4	0.0	1.0608588182563194E-4	0.0	0.0
78-79	7.956441136922395E-4	0.0	1.0608588182563194E-4	1.591288227384479E-4	0.0
80-81	9.812944068870954E-4	0.0	1.0608588182563194E-4	1.591288227384479E-4	0.0
82-83	0.001326073522820399	0.0	1.0608588182563194E-4	1.591288227384479E-4	0.0
84-85	0.001485202345558847	0.0	1.0608588182563194E-4	1.591288227384479E-4	0.0
86-87	0.001485202345558847	5.304294091281597E-5	1.0608588182563194E-4	1.591288227384479E-4	0.0
88	0.001485202345558847	5.304294091281597E-5	1.0608588182563194E-4	1.591288227384479E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCAGC	60	0.0	88.2101	1
TCACCGG	8130	0.0	82.389206	8
CACCGGC	7930	0.0	80.79721	9
CTTCACC	9080	0.0	76.043785	6
CCTTCAC	9900	0.0	73.9176	5
GTGTGGC	3010	0.0	72.82614	7
CGGGCCC	580	0.0	71.21829	7
CGTAGTG	1405	0.0	71.1607	4
GCTGCTA	20	0.0021736193	70.4146	2
AGGCAGC	20	0.0021736193	70.4146	2
TTCACCG	10195	0.0	66.89833	7
AACGGGC	615	0.0	65.63875	5
TGCTGCC	65	2.564775E-10	65.13976	1
ACCTTCA	4320	0.0	64.976204	4
ACGGGCC	630	0.0	64.82098	6
GGGCCCG	640	0.0	64.54157	8
GCAGCCG	25	0.003272021	63.511272	1
AAGCTGC	25	0.003272021	63.511272	1
AGCAGCG	30	1.3796357E-4	62.59076	2
GCTGCCA	75	0.0	62.590755	2
>>END_MODULE