FastQCFastQC Report
Tue 7 Mar 2017
1SN_24A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_24A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1885265
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCAGC600.088.21011
TCACCGG81300.082.3892068
CACCGGC79300.080.797219
CTTCACC90800.076.0437856
CCTTCAC99000.073.91765
GTGTGGC30100.072.826147
CGGGCCC5800.071.218297
CGTAGTG14050.071.16074
GCTGCTA200.002173619370.41462
AGGCAGC200.002173619370.41462
TTCACCG101950.066.898337
AACGGGC6150.065.638755
TGCTGCC652.564775E-1065.139761
ACCTTCA43200.064.9762044
ACGGGCC6300.064.820986
GGGCCCG6400.064.541578
GCAGCCG250.00327202163.5112721
AAGCTGC250.00327202163.5112721
AGCAGCG301.3796357E-462.590762
GCTGCCA750.062.5907552
Produced by FastQC (version 0.11.5)