FastQCFastQC Report
Tue 7 Mar 2017
1SN_25A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_25A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3037089
Sequences flagged as poor quality0
Sequence length93
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA160810.5294872820651618No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG96180.31668482550231486No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA71290.23473134965751744No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA69200.22784976008276345No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT67230.22136328569890446No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG57570.1895565128318597No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG54880.1806993473026309No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG54860.18063349477081508No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA45350.14932061589238904No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT43610.14359144562441206No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT43510.1432621829653329No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA41240.1357879206042365No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC40200.13236358894981345No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC38880.12801732184996883No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA38870.12798439558406094No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC38070.12535029431142783No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA35970.11843577847076592No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA35950.11836992593895009No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG34830.11468218415726375No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG34300.11293709206414433No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC31980.10529819837350832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTC257.8039375E-786.999663
GCAGCCC257.8039375E-786.999663
AGCAGCC1000.086.999662
GCTGCAC302.2702807E-886.999653
TGCTGCC302.2702807E-886.999652
AGCTGCA1550.086.999652
GCAGCTC302.2702807E-886.999653
GCAGCCG302.2702807E-886.999653
AGCAGCG900.086.999652
CAGCAGC4700.085.148591
CAGCTGC4150.084.903281
CTGCTGC1150.083.2170641
CAGCCCT176900.082.499671
AGCTGCT900.082.1663362
CAGCGTA72100.081.268051
GCTGCCC750.081.199683
AGCCCTT150750.080.074312
GCTGCAA600.079.749683
AGCAGCA2150.078.906662
GCTGCTT501.8189894E-1278.299693
Produced by FastQC (version 0.11.5)