FastQCFastQC Report
Tue 7 Mar 2017
1SN_25A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_25A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3037089
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG113500.078.7399448
CACCGGC114250.075.347219
CTTCACC128500.074.3701556
CCTTCAC142300.070.918185
CGTAGTG30600.069.026394
GTGTGGC52200.068.248857
CAGCGTA12450.066.369591
TTCACCG144250.064.623057
GCCTTCA45550.064.0952764
TGTGGCA59000.061.973998
CGGGGCA11200.061.6060594
ACCTTCA56550.060.4258544
TGTGTGG57550.058.560286
GCAGCCA1050.058.117472
GCGTAGT19350.057.732463
ATGCAGC900.057.3723752
GGGCCCG7950.057.270198
GCAGCCC1000.056.325534
CGGGCCC8100.056.2096337
TAGTGTG32550.055.80646
Produced by FastQC (version 0.11.5)