##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1SN_25A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3037089
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.760330039718955	12.0	12.0	12.0	2.0	27.0
2	14.594922308829277	12.0	12.0	12.0	12.0	22.0
3	13.722478333693877	12.0	12.0	12.0	12.0	22.0
4	13.757776937060456	12.0	12.0	12.0	12.0	22.0
5	22.42264978076046	22.0	22.0	22.0	22.0	27.0
6	26.846771365607	27.0	22.0	32.0	22.0	32.0
7	34.94013807300346	37.0	37.0	37.0	27.0	41.0
8	37.53304628214715	41.0	37.0	41.0	27.0	41.0
9	38.47736105198103	41.0	37.0	41.0	32.0	41.0
10-11	38.8523764038525	41.0	41.0	41.0	34.5	41.0
12-13	39.02813829295091	41.0	41.0	41.0	37.0	41.0
14-15	38.982159067449125	41.0	41.0	41.0	37.0	41.0
16-17	38.89023930480799	41.0	41.0	41.0	37.0	41.0
18-19	38.81646668899067	41.0	41.0	41.0	37.0	41.0
20-21	38.83326764543285	41.0	41.0	41.0	37.0	41.0
22-23	38.820430682143325	41.0	41.0	41.0	34.5	41.0
24-25	38.83275169084607	41.0	41.0	41.0	37.0	41.0
26-27	38.79738772884166	41.0	41.0	41.0	34.5	41.0
28-29	38.74470306928772	41.0	41.0	41.0	32.0	41.0
30-31	38.631131158816885	41.0	41.0	41.0	32.0	41.0
32-33	38.68113265696198	41.0	41.0	41.0	32.0	41.0
34-35	38.645339171818804	41.0	41.0	41.0	32.0	41.0
36-37	38.57010446516385	41.0	41.0	41.0	32.0	41.0
38-39	38.481186260922875	41.0	41.0	41.0	32.0	41.0
40-41	38.523625089682916	41.0	41.0	41.0	32.0	41.0
42-43	38.268073309672516	41.0	41.0	41.0	32.0	41.0
44-45	38.371084778878725	41.0	41.0	41.0	32.0	41.0
46-47	38.37218188205877	41.0	41.0	41.0	32.0	41.0
48-49	38.33023480708007	41.0	41.0	41.0	32.0	41.0
50-51	38.21634861540113	41.0	41.0	41.0	32.0	41.0
52-53	38.19039563871853	41.0	41.0	41.0	32.0	41.0
54-55	38.05203304875162	41.0	37.0	41.0	32.0	41.0
56-57	37.8518591322151	41.0	37.0	41.0	27.0	41.0
58-59	37.840541222203235	41.0	37.0	41.0	27.0	41.0
60-61	37.85919131773879	41.0	37.0	41.0	29.5	41.0
62-63	37.85397069364777	41.0	37.0	41.0	29.5	41.0
64-65	37.83145423133797	41.0	37.0	41.0	27.0	41.0
66-67	37.709162622498056	41.0	37.0	41.0	27.0	41.0
68-69	37.71141560224281	41.0	37.0	41.0	27.0	41.0
70-71	37.68441277157173	41.0	37.0	41.0	27.0	41.0
72-73	37.56180144868985	41.0	37.0	41.0	27.0	41.0
74-75	37.44998895323779	41.0	37.0	41.0	27.0	41.0
76-77	36.40013825739055	39.0	34.5	41.0	24.5	41.0
78-79	36.93233191388201	41.0	37.0	41.0	27.0	41.0
80-81	37.60237977879476	41.0	37.0	41.0	27.0	41.0
82-83	37.78008991504694	41.0	37.0	41.0	27.0	41.0
84-85	37.700476179657564	41.0	37.0	41.0	27.0	41.0
86-87	37.56406414168304	41.0	37.0	41.0	27.0	41.0
88-89	37.47763466925072	41.0	37.0	41.0	27.0	41.0
90-91	37.32446151561577	41.0	37.0	41.0	27.0	41.0
92-93	37.206078254539136	41.0	37.0	41.0	27.0	41.0
94-95	37.05339142185165	41.0	37.0	41.0	27.0	41.0
96-97	36.98301910151464	41.0	37.0	41.0	27.0	41.0
98-99	36.87520698273906	41.0	37.0	41.0	27.0	41.0
100	36.748840748493045	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.3099639354371355
1101	2	-0.4935450269900876
1101	3	-0.0033632895932349527
1101	4	0.10348383138843253
1101	5	-1.144232389732455
1101	6	-0.6158025872889752
1101	7	-0.356768148810076
1101	8	-0.0037002571796307393
1101	9	-0.02804867426748814
1101	10-11	-0.027089043879946928
1101	12-13	0.09557801308920943
1101	14-15	0.03175693312981309
1101	16-17	-0.028253426873313003
1101	18-19	0.12142340384744443
1101	20-21	0.15949826800148514
1101	22-23	0.20436573568449035
1101	24-25	0.1515887105622511
1101	26-27	0.223240837586971
1101	28-29	0.07074229341358063
1101	30-31	-0.016479917715649606
1101	32-33	-0.015553422679836615
1101	34-35	0.16309188455200996
1101	36-37	0.17499393481874392
1101	38-39	0.09381080698084787
1101	40-41	0.14691375285188712
1101	42-43	0.08795470004626793
1101	44-45	0.11487675310603152
1101	46-47	0.2725974815508394
1101	48-49	0.24250433603839383
1101	50-51	0.22586865133724388
1101	52-53	0.2091937630528875
1101	54-55	0.02280578966716007
1101	56-57	0.24439873172096327
1101	58-59	0.32682026777274586
1101	60-61	0.29255115914102703
1101	62-63	0.055077655400957326
1101	64-65	0.23189793049261453
1101	66-67	0.2572434722049408
1101	68-69	0.11577425652230744
1101	70-71	0.02877520521467858
1101	72-73	0.0031124602965846293
1101	74-75	0.10802796564507133
1101	76-77	-0.27540245162473553
1101	78-79	0.03813088106770124
1101	80-81	0.15779735303214437
1101	82-83	0.20360987154622734
1101	84-85	0.2624129649666287
1101	86-87	0.05563286224071362
1101	88-89	-0.059884331428612825
1101	90-91	-0.038431611119349895
1101	92-93	0.3052192846368271
1101	94-95	0.17249184546867724
1101	96-97	0.10646675573739373
1101	98-99	-0.04138737255571101
1101	100	-0.37353049655466464
1102	1	1.388396433606255
1102	2	0.8041902537840446
1102	3	-0.11974825507680364
1102	4	0.059852009614266066
1102	5	-0.7687010997777222
1102	6	-0.21332418165487965
1102	7	0.07633808465764957
1102	8	-0.1062477179675625
1102	9	-0.23666407105873333
1102	10-11	-0.21185561070739567
1102	12-13	-0.17337007460136533
1102	14-15	-0.03907058058368307
1102	16-17	-0.09432287885741886
1102	18-19	-0.06758289304624299
1102	20-21	-0.10184873665458838
1102	22-23	-0.17085135053358158
1102	24-25	-0.003207139217423105
1102	26-27	-0.103634436209191
1102	28-29	-0.18371103945901268
1102	30-31	-0.1318379734257178
1102	32-33	-0.21315232091851755
1102	34-35	-0.08692009838429016
1102	36-37	-0.05248859517482174
1102	38-39	-0.0031403438826487218
1102	40-41	-0.013957724304177077
1102	42-43	-0.08164509217519367
1102	44-45	-0.00797453921184399
1102	46-47	-0.3725507111436954
1102	48-49	-0.09877958263815145
1102	50-51	-0.07008646671419427
1102	52-53	-0.1335400909158082
1102	54-55	-0.1526106034355763
1102	56-57	-0.19440970738732943
1102	58-59	-0.1134262630278613
1102	60-61	0.016443545684914795
1102	62-63	-0.12695122849164164
1102	64-65	-0.09410593302899173
1102	66-67	-0.16086639515788193
1102	68-69	-0.2591087008440809
1102	70-71	-0.17114782532007666
1102	72-73	-0.14712639027786878
1102	74-75	-0.26365485104721387
1102	76-77	-0.07720747197343059
1102	78-79	-0.3616092842856773
1102	80-81	-0.28315895773945243
1102	82-83	-0.28081008188195256
1102	84-85	-0.1223270721971943
1102	86-87	-0.12299649049123218
1102	88-89	-0.232106435988932
1102	90-91	-0.3281581103518789
1102	92-93	-0.11391080434901824
1102	94-95	-0.052194619609707615
1102	96-97	0.05603447827723329
1102	98-99	-0.07653778348682039
1102	100	-0.3385194220921406
1103	1	1.250374859247147
1103	2	0.6837391640001034
1103	3	0.6100591967861622
1103	4	0.9745648832349705
1103	5	2.8981437723598518
1103	6	0.02402366105292586
1103	7	-0.5018872187946499
1103	8	-0.35583856395491864
1103	9	-0.4076217530079589
1103	10-11	-0.28588934902703045
1103	12-13	-0.4623099819920071
1103	14-15	-0.47435483268118617
1103	16-17	-0.424798888664462
1103	18-19	-0.3645483700476788
1103	20-21	-0.17030708687433105
1103	22-23	-0.1770070561266408
1103	24-25	-0.44751577384734276
1103	26-27	-0.36095983077622407
1103	28-29	-0.31867049771437905
1103	30-31	-0.25428798247970263
1103	32-33	-0.29687511943423317
1103	34-35	-0.5027586844675938
1103	36-37	-0.3295950844408182
1103	38-39	-0.2905199474042206
1103	40-41	-0.3264348378354285
1103	42-43	-0.2486729123486242
1103	44-45	-0.5210025088479284
1103	46-47	-0.7058896493265152
1103	48-49	-0.5028354816703811
1103	50-51	-0.5052739992295017
1103	52-53	-0.23854766464832267
1103	54-55	-0.2611778314494728
1103	56-57	-0.28440749419034717
1103	58-59	-0.5990149015668038
1103	60-61	-0.3899044493598254
1103	62-63	-0.36952738977333865
1103	64-65	-0.5415084445764151
1103	66-67	-0.6767210772192058
1103	68-69	-0.6288604778687912
1103	70-71	-0.6059517849306673
1103	72-73	-0.5702152084594303
1103	74-75	-0.49196207153050153
1103	76-77	-0.745859261357559
1103	78-79	-0.69397930207748
1103	80-81	-0.3116300122846951
1103	82-83	-0.5161074119615989
1103	84-85	-0.46875103450960864
1103	86-87	-0.40516171570558157
1103	88-89	-0.5898698711005821
1103	90-91	-0.8174131827406654
1103	92-93	-0.5416252042133536
1103	94-95	-0.6126416279135114
1103	96-97	-0.4107908487441776
1103	98-99	-0.5158244729752823
1103	100	-0.49940964464777693
1104	1	1.8470554908939416
1104	2	-0.5810941514294097
1104	3	0.007566984948155664
1104	4	-0.00237499573520239
1104	5	-1.0665928624380143
1104	6	-0.5362033593932374
1104	7	-0.21000695954732862
1104	8	-0.05757022149063573
1104	9	0.013721749695520202
1104	10-11	0.10917277723071095
1104	12-13	0.1432233728259149
1104	14-15	-0.00707907803514729
1104	16-17	0.14188460759363153
1104	18-19	0.046877848307318004
1104	20-21	0.08298829035917521
1104	22-23	0.12527747139152723
1104	24-25	0.07272950849590387
1104	26-27	-0.07942833795565463
1104	28-29	0.12842979948904087
1104	30-31	0.15246677098468098
1104	32-33	0.08902490563111343
1104	34-35	0.07812361581528648
1104	36-37	0.21553364402983277
1104	38-39	0.0683252561622183
1104	40-41	-0.009527901895296509
1104	42-43	0.04245359604826149
1104	44-45	0.06278665575979403
1104	46-47	-0.12504315668498123
1104	48-49	-0.12326374106093851
1104	50-51	0.15260081725633512
1104	52-53	0.019181056660805496
1104	54-55	0.015591961224288298
1104	56-57	0.09476284275955038
1104	58-59	-0.012688765569379257
1104	60-61	-0.003318707962613132
1104	62-63	0.10106021362122419
1104	64-65	-0.07231698111811369
1104	66-67	-0.4971285681777715
1104	68-69	-0.19652546157632145
1104	70-71	-0.01978570909008681
1104	72-73	-0.004941100964884981
1104	74-75	-0.1332647535228375
1104	76-77	-0.29563116668214917
1104	78-79	-0.08945393236358257
1104	80-81	0.01014067558009657
1104	82-83	-0.11706419213849273
1104	84-85	-0.06050131201882891
1104	86-87	-0.15291263775234398
1104	88-89	0.028563544590326728
1104	90-91	-0.00660536235179876
1104	92-93	-0.03338335940367898
1104	94-95	-0.09812986510332422
1104	96-97	-0.23247201750558588
1104	98-99	-0.2252203980657015
1104	100	0.01454674164505576
1105	1	1.5643195352607897
1105	2	0.5357453953166793
1105	3	-0.040123953396989265
1105	4	-0.1515140187060009
1105	5	-1.1286550992666058
1105	6	0.47988236173921095
1105	7	0.08410073960710207
1105	8	0.03699963233155046
1105	9	0.01909712198678193
1105	10-11	0.016392660151531402
1105	12-13	-0.05363876814837454
1105	14-15	0.03203016163483596
1105	16-17	0.010692248550810746
1105	18-19	-0.09687964030997875
1105	20-21	-0.01967132416960027
1105	22-23	0.029647400527814227
1105	24-25	-0.007934282541711468
1105	26-27	-0.024306637226480632
1105	28-29	-0.06071504320720322
1105	30-31	0.003366432071267411
1105	32-33	-0.16011035163498377
1105	34-35	4.0118248537623913E-4
1105	36-37	-0.060835874302298976
1105	38-39	-0.025223749349400748
1105	40-41	0.053965987392977866
1105	42-43	0.1903612335820668
1105	44-45	0.03676769030158056
1105	46-47	-0.2525550528884679
1105	48-49	-0.13382996611314724
1105	50-51	-0.05940014128668025
1105	52-53	-0.07927226571775492
1105	54-55	-0.08532207800751479
1105	56-57	-0.07697622813839189
1105	58-59	-0.06486055017106906
1105	60-61	-0.1783996706136719
1105	62-63	-0.26109616582487405
1105	64-65	-0.14218210042248103
1105	66-67	-0.2559451098915133
1105	68-69	-0.20932235315825665
1105	70-71	-0.29541749163774966
1105	72-73	-0.1729456198105268
1105	74-75	-0.1968544971125823
1105	76-77	-0.18024518195037587
1105	78-79	-0.07367699901655556
1105	80-81	-0.3594553670066034
1105	82-83	-0.18588552656729718
1105	84-85	-0.26257655764548105
1105	86-87	-0.308574203600827
1105	88-89	-0.14994523634836554
1105	90-91	-0.28361881618768336
1105	92-93	0.02210053365030973
1105	94-95	0.038602530227201726
1105	96-97	0.02313614380350515
1105	98-99	-0.1755798516961775
1105	100	-0.10307264831077845
1106	1	1.2760288208544939
1106	2	-0.6429459450425465
1106	3	-0.11939703340159724
1106	4	-0.16302421053806526
1106	5	-1.1641945415361157
1106	6	-0.8421026271806724
1106	7	-0.15328735388926162
1106	8	-0.0186903494740136
1106	9	-0.1436712727494296
1106	10-11	-0.22792633467953038
1106	12-13	-0.09272518770297467
1106	14-15	-0.08392735073817903
1106	16-17	-0.17771141070142704
1106	18-19	-0.10891797992695729
1106	20-21	-0.07956330977482651
1106	22-23	-0.16801331476274584
1106	24-25	-0.22803538358648723
1106	26-27	-0.37163454706352894
1106	28-29	-0.25959015005597763
1106	30-31	-0.11753117799971591
1106	32-33	-0.17001091193788653
1106	34-35	-0.2006138473518888
1106	36-37	-0.45603873289213936
1106	38-39	-0.3004014733566791
1106	40-41	-0.26931069934384055
1106	42-43	-0.20019132529529315
1106	44-45	-0.07867024898420283
1106	46-47	-0.40213194515006734
1106	48-49	-0.32996987838327385
1106	50-51	-0.372174699086834
1106	52-53	-0.4120909832109376
1106	54-55	-0.35629887005233485
1106	56-57	-0.3356156379848301
1106	58-59	-0.21650474845023382
1106	60-61	-0.3057646124263016
1106	62-63	-0.40205562090283564
1106	64-65	-0.6900211426287584
1106	66-67	-0.4687624072003942
1106	68-69	-0.5456489258783961
1106	70-71	-0.6120049763305815
1106	72-73	-0.46318734589716826
1106	74-75	-0.7105800424767565
1106	76-77	-0.4424342486749211
1106	78-79	-0.5066934704748718
1106	80-81	-0.3521379343782698
1106	82-83	-0.4040167248009112
1106	84-85	-0.5788577648557265
1106	86-87	-0.40167840493472795
1106	88-89	-0.5330139598706367
1106	90-91	-0.5343905389784211
1106	92-93	-0.5306814198265926
1106	94-95	-0.44706258495510554
1106	96-97	-0.4642382837684309
1106	98-99	-0.6274143010800088
1106	100	-0.8538110101683856
1107	1	1.0770686016482642
1107	2	-0.39423435236749427
1107	3	-0.07473700226360158
1107	4	-0.13707222144349807
1107	5	-1.231941909980275
1107	6	-0.39749522441556806
1107	7	-0.5260625154923915
1107	8	-0.3395560450493278
1107	9	-0.2820504176453795
1107	10-11	-0.2570544104073207
1107	12-13	-0.48694846920001567
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2227	100	0.6377223939499999
2228	1	1.360588420109469
2228	2	1.6278862949246378
2228	3	-0.28293738691320414
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2228	92-93	0.6061404566360622
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2228	98-99	0.7457570105630822
2228	100	0.5348265681389464
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	23.0
13	39.0
14	182.0
15	532.0
16	2017.0
17	5358.0
18	8720.0
19	11079.0
20	13129.0
21	14787.0
22	16983.0
23	19580.0
24	21793.0
25	24855.0
26	28162.0
27	31583.0
28	35339.0
29	40188.0
30	45255.0
31	52537.0
32	61361.0
33	73109.0
34	89367.0
35	115291.0
36	162195.0
37	266386.0
38	656185.0
39	1239561.0
40	1492.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.721858754202064	20.158466691947453	18.824317242398774	36.29535731145172
2	19.87120533354934	50.80102353464538	12.730500272150106	16.597270859655172
3	38.07030350444126	16.332086415643403	25.418155345464026	20.179454734451312
4	20.83765078995051	16.530236683877224	20.572726054455433	42.059386471716834
5	17.960421969853368	35.376605690514836	26.002464860265867	20.660507479365933
6	17.543805927320534	27.816339922866927	30.765776044100125	23.87407810571241
7	20.620403287490095	26.686540960768685	35.77903051244136	16.914025239299868
8	21.066784674403678	27.715552622922807	34.03074457152886	17.186918131144658
9	20.953781729807723	28.039382448127135	27.703073568143704	23.303762253921438
10-11	19.700048302832087	30.236502782763363	28.58380179178154	21.47964712262301
12-13	24.31871440053288	26.550868282095124	27.183925133573627	21.946492183798366
14-15	21.75102540623604	27.27611867811579	28.598141180584435	22.37471473506374
16-17	21.03216270580151	29.42674712529004	28.28810746079552	21.25298270811293
18-19	21.442341663349346	29.395779972203645	29.74308622500032	19.418792139446687
20-21	22.875687870852648	28.892073956344383	29.693466342277098	18.538771830525874
22-23	22.021662848866136	30.288888471822855	28.578846388762397	19.110602290548613
24-25	22.20382741500167	28.222518339106955	26.750072190838004	22.823582055053375
26-27	20.342489140094347	26.36702447639829	29.97696149174423	23.313524891763134
28-29	19.852269212007524	27.963584082531945	30.545983582577797	21.63816312288273
30-31	20.327125461811736	29.908392555164266	28.074169117561105	21.690312865462896
32-33	20.73643544854958	29.96069591638572	28.63814659366255	20.664722041402143
34-35	21.438950257960833	29.124994361376967	29.80479004731175	19.631265333350456
36-37	21.975977654918903	27.956194237310793	28.814911252189184	21.252916855581116
38-39	21.497551336166882	29.267307296239508	28.804409652807834	20.430731714785775
40-41	21.070952720960367	29.546728586534897	29.88223579938845	19.500082893116282
42-43	20.41722236664065	29.832301395532003	29.329253325268585	20.42122291255877
44-45	20.617077734633394	30.14113185356109	28.68152694899623	20.56026346280929
46-47	20.08811261688051	27.912387873320004	28.77627676449549	23.223222745303996
48-49	20.95108522520829	28.085236897113795	30.234581573767112	20.7290963039108
50-51	21.94499403869956	30.491845974879233	28.18868660088657	19.374473385534635
52-53	20.47604512809166	30.15703756679192	28.272787618486923	21.094129686629497
54-55	22.402965649289875	28.215179835218287	28.112133632712904	21.269720882778934
56-57	21.658950361924575	28.57586470279178	29.1635980092543	20.601586926029345
58-59	21.16441432689629	27.854321179545916	29.083272796007765	21.897991697550033
60-61	19.654303158655857	30.33909511198739	29.364097062872503	20.642504666484253
62-63	20.604805560225266	30.964762375352418	27.359579597864336	21.070852466557984
64-65	19.73892729377018	30.161273062821753	28.86043689919775	21.239362744210318
66-67	20.35294322952011	30.194110215406923	29.388174004778918	20.064772550294048
68-69	20.95569145323038	29.874708972967206	29.203951547024143	19.965648026778275
70-71	19.843985474248534	30.86037320605356	29.040291542328855	20.25534977736905
72-73	20.165000103717738	30.723334087344824	28.28814038706143	20.823525421876013
74-75	20.135810968990373	29.073036713774275	30.29494690474991	20.496205412485445
76-77	19.679469386639642	30.48583693135104	28.555369961170058	21.279323720839262
78-79	19.61564182017715	29.352251448673382	28.457677730221278	22.57442900092819
80-81	20.91258214495443	29.29262281223232	28.404616860473634	21.390178182339614
82-83	20.419289655324555	28.005419004843123	29.63948372932107	21.935807610511247
84-85	20.943837997503532	28.945463896514063	28.330104912961062	21.780593193021343
86-87	21.639224270345718	29.476284692348496	28.57354525995122	20.310945777354565
88-89	21.411539141592492	28.95805819322384	28.236133350059877	21.39426931512379
90-91	21.298223397470405	30.57645001512962	27.413503522616555	20.711823064783417
92-93	21.651044799806655	30.010710914299842	28.14830253575052	20.18994175014298
94-95	21.427310822962383	30.824351871150302	27.61705369845928	20.13128360742803
96-97	21.32295102316725	30.096385058192237	27.936454940898997	20.644208977741517
98-99	22.68925935328204	30.878993009424484	27.01035432284006	19.42139331445341
100	21.737986604936506	31.776348997345817	26.961969175088385	19.5236952226293
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	5.5
2	8.5
3	14.0
4	23.5
5	30.5
6	44.0
7	59.0
8	63.5
9	65.5
10	79.5
11	100.0
12	111.5
13	120.5
14	150.5
15	185.0
16	250.0
17	345.0
18	498.0
19	825.0
20	1339.5
21	2057.0
22	3216.5
23	5036.0
24	7619.5
25	11321.0
26	16203.0
27	21870.5
28	28108.0
29	35277.5
30	43977.0
31	54001.0
32	64849.5
33	76868.5
34	90404.5
35	105016.0
36	118859.0
37	129192.5
38	136721.5
39	142817.5
40	146194.0
41	146798.0
42	144804.5
43	142091.5
44	140361.5
45	136717.0
46	127301.0
47	115168.5
48	107377.0
49	104113.5
50	100611.5
51	93272.0
52	82788.0
53	70390.0
54	57859.5
55	46601.0
56	36059.5
57	26862.0
58	19551.0
59	14828.5
60	12267.5
61	10523.0
62	8869.5
63	7161.5
64	5767.0
65	5068.5
66	5142.5
67	4855.0
68	3701.5
69	3214.5
70	3587.5
71	3337.0
72	2187.0
73	1154.5
74	681.0
75	458.0
76	322.5
77	239.5
78	175.0
79	125.5
80	88.0
81	65.0
82	54.0
83	39.5
84	29.0
85	29.5
86	19.5
87	12.5
88	18.0
89	23.5
90	19.5
91	13.5
92	13.0
93	16.0
94	14.0
95	15.0
96	18.0
97	16.5
98	30.5
99	85.5
100	104.5
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.348432660353385
2	0.005334055077082035
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	3.457257920330949E-4
30-31	0.008972407459906509
32-33	0.0
34-35	0.0
36-37	0.0
38-39	8.06693514743888E-4
40-41	0.0014981450988100777
42-43	1.1524193067769828E-4
44-45	0.0
46-47	0.002189596682876267
48-49	1.6463132953956897E-5
50-51	0.0
52-53	0.0014816819658561209
54-55	0.019327718087945398
56-57	0.011112614743920906
58-59	0.01300587503362595
60-61	1.9755759544748275E-4
62-63	0.012429665380237457
64-65	1.3170506363165518E-4
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.1524193067769828E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3037089.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.05470717885981
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.18231092099406	63.82205799251667
2	8.60636391091847	12.746835185955483
3	2.355934296856298	5.234040734589785
4	0.9975486413821679	2.9549269013690354
5	0.5160506012761312	1.9107988083489218
6	0.3198814222801625	1.4213235035348781
7	0.22519430640208873	1.1673688893267182
8	0.15042592914170907	0.8911798507757744
9	0.10421666874623534	0.6945961398442818
>10	0.5067573109737723	6.330490012035023
>50	0.025067898492545804	1.2597451043698784
>100	0.009705952871989828	1.2794448929963909
>500	4.969613590686607E-4	0.2524639694884337
>1k	4.5178305307561486E-5	0.03472801484863361
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	6.585253181582759E-5	0.0
22-23	0.0	0.0	0.0	6.585253181582759E-5	0.0
24-25	0.0	0.0	0.0	6.585253181582759E-5	0.0
26-27	0.0	0.0	0.0	6.585253181582759E-5	0.0
28-29	0.0	0.0	0.0	6.585253181582759E-5	0.0
30-31	0.0	0.0	0.0	6.585253181582759E-5	0.0
32-33	0.0	0.0	0.0	6.585253181582759E-5	0.0
34-35	0.0	0.0	0.0	6.585253181582759E-5	0.0
36-37	0.0	0.0	0.0	6.585253181582759E-5	0.0
38-39	0.0	0.0	0.0	9.877879772374139E-5	0.0
40-41	1.6463132953956897E-5	0.0	0.0	9.877879772374139E-5	0.0
42-43	3.2926265907913794E-5	0.0	0.0	9.877879772374139E-5	0.0
44-45	3.2926265907913794E-5	0.0	0.0	9.877879772374139E-5	0.0
46-47	3.2926265907913794E-5	0.0	0.0	9.877879772374139E-5	0.0
48-49	4.9389398861870694E-5	0.0	0.0	9.877879772374139E-5	0.0
50-51	8.231566476978448E-5	0.0	0.0	9.877879772374139E-5	0.0
52-53	9.877879772374139E-5	0.0	0.0	9.877879772374139E-5	0.0
54-55	9.877879772374139E-5	3.2926265907913794E-5	0.0	9.877879772374139E-5	0.0
56-57	1.1524193067769828E-4	3.2926265907913794E-5	0.0	9.877879772374139E-5	0.0
58-59	1.3170506363165518E-4	3.2926265907913794E-5	0.0	9.877879772374139E-5	0.0
60-61	1.8109446249352587E-4	3.2926265907913794E-5	0.0	9.877879772374139E-5	0.0
62-63	2.3048386135539656E-4	3.2926265907913794E-5	0.0	9.877879772374139E-5	0.0
64-65	2.7987326021726726E-4	3.2926265907913794E-5	0.0	9.877879772374139E-5	0.0
66-67	3.4572579203309483E-4	3.2926265907913794E-5	0.0	9.877879772374139E-5	0.0
68-69	4.7743085566475003E-4	3.2926265907913794E-5	0.0	9.877879772374139E-5	0.0
70-71	5.597465204345345E-4	3.2926265907913794E-5	0.0	9.877879772374139E-5	0.0
72-73	5.597465204345345E-4	3.2926265907913794E-5	0.0	1.3170506363165518E-4	0.0
74-75	5.597465204345345E-4	3.2926265907913794E-5	0.0	1.3170506363165518E-4	0.0
76-77	6.091359192964053E-4	3.2926265907913794E-5	0.0	1.3170506363165518E-4	0.0
78-79	6.914515840661897E-4	3.2926265907913794E-5	0.0	1.3170506363165518E-4	1.6463132953956897E-5
80-81	7.737672488359743E-4	3.2926265907913794E-5	0.0	1.3170506363165518E-4	3.2926265907913794E-5
82-83	0.0010701036420071983	3.2926265907913794E-5	0.0	1.3170506363165518E-4	3.2926265907913794E-5
84-85	0.0011688824397309396	3.2926265907913794E-5	0.0	1.3170506363165518E-4	3.2926265907913794E-5
86-87	0.0012182718385928104	3.2926265907913794E-5	0.0	1.3170506363165518E-4	3.2926265907913794E-5
88	0.0012182718385928104	3.2926265907913794E-5	0.0	1.3170506363165518E-4	3.2926265907913794E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	11350	0.0	78.739944	8
CACCGGC	11425	0.0	75.34721	9
CTTCACC	12850	0.0	74.370155	6
CCTTCAC	14230	0.0	70.91818	5
CGTAGTG	3060	0.0	69.02639	4
GTGTGGC	5220	0.0	68.24885	7
CAGCGTA	1245	0.0	66.36959	1
TTCACCG	14425	0.0	64.62305	7
GCCTTCA	4555	0.0	64.095276	4
TGTGGCA	5900	0.0	61.97399	8
CGGGGCA	1120	0.0	61.60605	94
ACCTTCA	5655	0.0	60.425854	4
TGTGTGG	5755	0.0	58.56028	6
GCAGCCA	105	0.0	58.11747	2
GCGTAGT	1935	0.0	57.73246	3
ATGCAGC	90	0.0	57.372375	2
GGGCCCG	795	0.0	57.27019	8
GCAGCCC	100	0.0	56.32553	4
CGGGCCC	810	0.0	56.209633	7
TAGTGTG	3255	0.0	55.8064	6
>>END_MODULE