FastQCFastQC Report
Tue 7 Mar 2017
1SN_26A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_26A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2758873
Sequences flagged as poor quality0
Sequence length93
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA155060.5620410943164111No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG86820.3146937173258791No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA65690.2381044723696959No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT58080.21052074524633793No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA55250.2002629334514492No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG53050.19228866279817883No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG49980.1811609305683879No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG48290.17503524083928473No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA40760.1477414871942275No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT40550.14698030681368804No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA40470.1466903333353873No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT40120.1454216993678216No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC39310.14248571790002657No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA38620.13998469664968266No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC32810.11892537278809137No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA31460.11403207034176638No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC28760.10424546544911636No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA28250.10239688452494913No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCG159.388564E-486.999883
GCTGCCC950.086.999883
GCTGCAG302.2700988E-886.999883
AGCTGCA1200.086.999882
TGCAGCT356.6393113E-1086.999882
TGCTGCA450.086.999872
AGCTGCG900.086.999872
GCAGCCT202.6992171E-586.999873
GCAGCCG257.8036464E-786.999873
GCAGCCC402.0008883E-1186.999873
GCAGCAC257.8036464E-786.999873
CAGCTGC5500.085.418061
AGCTGCC1950.084.769112
AGCAGCA1750.084.514162
CAGCAGC4950.084.363511
AGCAGCC950.082.420942
AGCAGCG1700.081.882232
CAGCCCT160550.081.201691
CAGCGTA63400.080.413131
AGCCCTT134600.080.1807862
Produced by FastQC (version 0.11.5)