FastQCFastQC Report
Tue 7 Mar 2017
1SN_26A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_26A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2758873
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG106800.080.2520458
CACCGGC107750.076.8436369
CTTCACC121300.073.715836
CGTAGTG23650.069.6630554
CCTTCAC137350.069.134335
GGGCCCG7500.068.842728
CGGGCCC7550.068.386817
GTGTGGC47300.068.3737
GGCGCGC2850.065.87826
TTCACCG136100.064.596097
CGGGGCA10650.062.5842994
CAGCGTA9950.061.1626931
TGTGGCA53850.060.5795028
CAGCTGC704.965841E-1060.478891
AGGCTGC551.3003955E-859.741712
ACCTTCA56850.059.1165584
TGTGTGG51400.058.992396
GCTGCCT1800.058.798921
AACGGGC8900.058.540925
GGCTGCT1200.057.328941
Produced by FastQC (version 0.11.5)