##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1SN_26A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2758873 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.799700094930067 12.0 12.0 12.0 2.0 27.0 2 14.67441089169382 12.0 12.0 12.0 12.0 22.0 3 13.729191593813852 12.0 12.0 12.0 12.0 22.0 4 13.7913927172436 12.0 12.0 12.0 12.0 22.0 5 22.44495161611281 22.0 22.0 22.0 22.0 27.0 6 26.85171481253396 27.0 22.0 32.0 22.0 32.0 7 34.981583421926274 37.0 37.0 37.0 27.0 41.0 8 37.596355468337975 41.0 37.0 41.0 27.0 41.0 9 38.55106487322903 41.0 41.0 41.0 32.0 41.0 10-11 38.9125137329627 41.0 41.0 41.0 37.0 41.0 12-13 39.08938921798865 41.0 41.0 41.0 37.0 41.0 14-15 39.04542724511059 41.0 41.0 41.0 37.0 41.0 16-17 38.94455942698341 41.0 41.0 41.0 37.0 41.0 18-19 38.88871869056676 41.0 41.0 41.0 37.0 41.0 20-21 38.90948187901364 41.0 41.0 41.0 37.0 41.0 22-23 38.88626841467513 41.0 41.0 41.0 37.0 41.0 24-25 38.90656402088824 41.0 41.0 41.0 37.0 41.0 26-27 38.876629152556134 41.0 41.0 41.0 37.0 41.0 28-29 38.824166244694844 41.0 41.0 41.0 37.0 41.0 30-31 38.693953835497325 41.0 41.0 41.0 32.0 41.0 32-33 38.738193276747424 41.0 41.0 41.0 32.0 41.0 34-35 38.713938626388384 41.0 41.0 41.0 32.0 41.0 36-37 38.64172888712166 41.0 41.0 41.0 32.0 41.0 38-39 38.56116211221031 41.0 41.0 41.0 32.0 41.0 40-41 38.595872843730035 41.0 41.0 41.0 32.0 41.0 42-43 38.33290133326181 41.0 41.0 41.0 32.0 41.0 44-45 38.44821744241217 41.0 41.0 41.0 32.0 41.0 46-47 38.44670124358751 41.0 41.0 41.0 32.0 41.0 48-49 38.40179359470335 41.0 41.0 41.0 32.0 41.0 50-51 38.29499853744627 41.0 41.0 41.0 32.0 41.0 52-53 38.27543221453108 41.0 41.0 41.0 32.0 41.0 54-55 38.13449894214051 41.0 39.0 41.0 32.0 41.0 56-57 37.921887488115615 41.0 37.0 41.0 29.5 41.0 58-59 37.91903723005735 41.0 37.0 41.0 29.5 41.0 60-61 37.932207100508066 41.0 37.0 41.0 32.0 41.0 62-63 37.92538674306501 41.0 37.0 41.0 32.0 41.0 64-65 37.908911356195084 41.0 37.0 41.0 29.5 41.0 66-67 37.78378453810669 41.0 37.0 41.0 27.0 41.0 68-69 37.797307813734086 41.0 37.0 41.0 27.0 41.0 70-71 37.78031246817088 41.0 37.0 41.0 27.0 41.0 72-73 37.63265199231715 41.0 37.0 41.0 27.0 41.0 74-75 37.55006555212944 41.0 37.0 41.0 27.0 41.0 76-77 36.50827094976826 39.0 34.5 41.0 24.5 41.0 78-79 37.02193087539731 41.0 37.0 41.0 27.0 41.0 80-81 37.65867819939518 41.0 37.0 41.0 27.0 41.0 82-83 37.84320445341268 41.0 37.0 41.0 32.0 41.0 84-85 37.747219788660075 41.0 37.0 41.0 27.0 41.0 86-87 37.5916078775645 41.0 37.0 41.0 27.0 41.0 88-89 37.496579037889745 41.0 37.0 41.0 27.0 41.0 90-91 37.346363170758494 41.0 37.0 41.0 27.0 41.0 92-93 37.253694352730264 41.0 37.0 41.0 27.0 41.0 94-95 37.09262967160866 41.0 37.0 41.0 27.0 41.0 96-97 37.04531397422063 41.0 37.0 41.0 27.0 41.0 98-99 36.91848936141678 41.0 37.0 41.0 27.0 41.0 100 36.7846008134481 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2947424542899135 1101 2 -0.5507187601476762 1101 3 0.11231490666019006 1101 4 -0.010381657602783534 1101 5 -1.1216432780237007 1101 6 -0.7415148738301234 1101 7 -0.47626813442283833 1101 8 -0.2027567137845594 1101 9 -0.17207899518768954 1101 10-11 -0.17647891886544897 1101 12-13 -0.26608589775896974 1101 14-15 -0.18755784370293327 1101 16-17 -0.22455565378787412 1101 18-19 -0.16782499929671957 1101 20-21 -0.06148252369462881 1101 22-23 -0.20084229777675233 1101 24-25 -0.14267177639947448 1101 26-27 -0.07906867030538933 1101 28-29 -0.198430339382206 1101 30-31 0.04795155027906617 1101 32-33 -0.2978424374206341 1101 34-35 -0.12352640772557777 1101 36-37 -0.08882180485324653 1101 38-39 -0.16013797596961155 1101 40-41 -0.05019054048839422 1101 42-43 -0.16793493860341613 1101 44-45 -0.2524046950815233 1101 46-47 -0.21677872162062073 1101 48-49 -0.17406113071099583 1101 50-51 -0.14539513633145873 1101 52-53 -0.09467849823918328 1101 54-55 -0.08572866640052013 1101 56-57 -0.06907608576882751 1101 58-59 -0.15149193897558888 1101 60-61 -0.1991455903776469 1101 62-63 -0.30375834098583 1101 64-65 -0.2722080518313774 1101 66-67 -0.19838791954926904 1101 68-69 -0.03253774306431012 1101 70-71 -0.2204896127906082 1101 72-73 -0.053099961604409884 1101 74-75 -0.2408679947982577 1101 76-77 -0.527906993184601 1101 78-79 -0.15155399098927802 1101 80-81 -0.03502293351159835 1101 82-83 -0.15439808137077193 1101 84-85 -0.04088363095400638 1101 86-87 -0.09474445964664824 1101 88-89 -0.34948513548316384 1101 90-91 -0.18189242085239954 1101 92-93 -0.2458981349920606 1101 94-95 -0.044285737737027375 1101 96-97 -0.22300232204938197 1101 98-99 -0.4127952669317523 1101 100 -0.6349840731027143 1102 1 1.5693496303991576 1102 2 0.6077622360852821 1102 3 -0.08511538432245302 1102 4 -0.0912339510040372 1102 5 -0.7077192328940995 1102 6 -0.21935256325243557 1102 7 -0.05557234184562532 1102 8 -0.35921807484275803 1102 9 -0.15441044812106242 1102 10-11 -0.13704126970875308 1102 12-13 -0.14703736698587022 1102 14-15 0.01206214115399007 1102 16-17 -0.07347680942453394 1102 18-19 0.017691735014402354 1102 20-21 -0.08241077999264945 1102 22-23 -0.08461879817272688 1102 24-25 -0.06990563202649724 1102 26-27 0.13083819371185967 1102 28-29 -0.06437174554859837 1102 30-31 -0.14190694817452254 1102 32-33 -0.17664643482009268 1102 34-35 -0.03655032898154076 1102 36-37 -0.13764244693603445 1102 38-39 -0.057481047072542424 1102 40-41 0.10105944947864742 1102 42-43 -0.03893070001156218 1102 44-45 0.17079248204439068 1102 46-47 -0.258301303810633 1102 48-49 -0.06762286218614122 1102 50-51 -0.11774086616921409 1102 52-53 -0.07879974791813282 1102 54-55 -0.13568336757879962 1102 56-57 -0.03320674302145221 1102 58-59 0.07158669870874945 1102 60-61 -0.03030455797252074 1102 62-63 -0.28435241939811107 1102 64-65 -0.2690281046448817 1102 66-67 -0.15372543841123587 1102 68-69 -0.195698781462454 1102 70-71 -0.27876974913186814 1102 72-73 -0.032690409422308164 1102 74-75 -0.062118576710027185 1102 76-77 -0.16077350396133738 1102 78-79 -0.3569754522968225 1102 80-81 -0.18675994015742958 1102 82-83 -0.292970922678748 1102 84-85 -0.4059423873215877 1102 86-87 -0.294455493630295 1102 88-89 -0.36610992010892573 1102 90-91 -0.37763082007329984 1102 92-93 -0.34375303763220444 1102 94-95 -0.2958472846056708 1102 96-97 -0.31109791209453874 1102 98-99 -0.5988674092767354 1102 100 -0.6284154931279673 1103 1 1.6174692961175072 1103 2 0.846471339925964 1103 3 0.3754109314670213 1103 4 0.9854018953686499 1103 5 2.9823256639764537 1103 6 -0.031730319297956555 1103 7 -0.6049588994558661 1103 8 -0.12026448309311633 1103 9 -0.2385476280641612 1103 10-11 -0.18846707874146773 1103 12-13 -0.42751500745528404 1103 14-15 -0.35122466538939534 1103 16-17 -0.2380838506763041 1103 18-19 -0.1979632703198746 1103 20-21 -0.11715028923874371 1103 22-23 -0.23180051936760293 1103 24-25 -0.21813753992123708 1103 26-27 -0.2668047205840125 1103 28-29 -0.2036187227889883 1103 30-31 -0.07256684326698348 1103 32-33 -0.314059752743276 1103 34-35 -0.28681704306404754 1103 36-37 -0.19702522609677686 1103 38-39 -0.05691427964722351 1103 40-41 -0.29244939653842295 1103 42-43 -0.35510357518937496 1103 44-45 -0.5743733252102317 1103 46-47 -0.3990930001122024 1103 48-49 -0.4216357978191425 1103 50-51 -0.14532910407817923 1103 52-53 -0.09359682295369254 1103 54-55 -0.21668110050909917 1103 56-57 -0.3141678027654109 1103 58-59 -0.4618025704946618 1103 60-61 -0.2558891063366744 1103 62-63 -0.5017021704649665 1103 64-65 -0.2505025889976551 1103 66-67 -0.4079984202746729 1103 68-69 -0.5603858369389911 1103 70-71 -0.4819947669128055 1103 72-73 -0.26572072592302476 1103 74-75 -0.39808941597034675 1103 76-77 -0.5117025580153864 1103 78-79 -0.5472815067064971 1103 80-81 -0.28501074029967555 1103 82-83 -0.365067073105493 1103 84-85 -0.46890358042258384 1103 86-87 -0.37234156226472237 1103 88-89 -0.40859525083438086 1103 90-91 -0.6607033663037427 1103 92-93 -0.41128170939607855 1103 94-95 -0.3130558994705339 1103 96-97 -0.21801469730080214 1103 98-99 -0.36427014754131193 1103 100 -0.44140446894589047 1104 1 1.739040947930679 1104 2 -0.45583709050488075 1104 3 -0.04178367962110485 1104 4 0.15727986560201934 1104 5 -1.160337440273846 1104 6 -0.5629839884097656 1104 7 -0.4144444620250596 1104 8 -0.15546192324116248 1104 9 -0.29143822423542787 1104 10-11 -0.09808637170013412 1104 12-13 -0.06655643627031793 1104 14-15 -0.16139311955415536 1104 16-17 -0.14047840040290538 1104 18-19 -0.10686909575122883 1104 20-21 -0.15016294802392594 1104 22-23 -0.047246117272088384 1104 24-25 -0.11409908832835214 1104 26-27 -0.17177271544586148 1104 28-29 -0.042156400024566665 1104 30-31 -0.117234959873457 1104 32-33 -0.2025690729861509 1104 34-35 -0.1733190097079742 1104 36-37 -0.2748074262220115 1104 38-39 -0.22961054917389134 1104 40-41 -0.1483259857879986 1104 42-43 -0.07315094027787694 1104 44-45 -0.13485688619157088 1104 46-47 -0.17897983771521098 1104 48-49 -0.3608165131072525 1104 50-51 -0.31943675861418086 1104 52-53 -0.13266683589692008 1104 54-55 -0.24126388755244932 1104 56-57 -0.15870605028578666 1104 58-59 -0.21275330725232067 1104 60-61 -0.12838755832093085 1104 62-63 -0.20530144684622798 1104 64-65 -0.2827458699803387 1104 66-67 -0.4911903763236083 1104 68-69 -0.49947596010932216 1104 70-71 -0.4139260161440319 1104 72-73 -0.42047896989309663 1104 74-75 -0.2932947779055084 1104 76-77 -0.5442421634565733 1104 78-79 -0.1834433816296226 1104 80-81 -0.31589644991289134 1104 82-83 -0.48580653541637986 1104 84-85 -0.19042716687105354 1104 86-87 -0.38791143822777485 1104 88-89 -0.2547184672966054 1104 90-91 -0.2729603662701905 1104 92-93 -0.3279219351617684 1104 94-95 -0.3577444582713696 1104 96-97 -0.32319441738600574 1104 98-99 -0.2508352753242491 1104 100 -0.46279416775723803 1105 1 1.7237999738101255 1105 2 0.28730559769119246 1105 3 -0.07805442754871095 1105 4 -0.3172907226224755 1105 5 -1.0859100157331056 1105 6 0.33870004576860424 1105 7 0.17571753257317368 1105 8 -0.020734682770402912 1105 9 0.0867410468047396 1105 10-11 -0.028132770552687703 1105 12-13 -0.056047807979688 1105 14-15 0.08028438563924567 1105 16-17 0.013085345572179108 1105 18-19 0.07130168026503725 1105 20-21 0.1729900143110754 1105 22-23 0.11967058793373297 1105 24-25 0.11033727351159683 1105 26-27 -0.020948906747790375 1105 28-29 0.10461733509724525 1105 30-31 0.003118917120275455 1105 32-33 -0.05105047480861913 1105 34-35 0.06001509691174789 1105 36-37 0.018705663374788628 1105 38-39 -0.03867670905423637 1105 40-41 -0.10187355950736077 1105 42-43 0.07263001194253604 1105 44-45 0.04030875425729619 1105 46-47 -0.06629751806298856 1105 48-49 0.0014777308214419804 1105 50-51 -0.00855835478411393 1105 52-53 -0.0982767594200098 1105 54-55 -0.06868480664059717 1105 56-57 0.0021864479405167003 1105 58-59 -0.04023956625908198 1105 60-61 -0.06079720940242339 1105 62-63 -0.2242370887787999 1105 64-65 -0.08822067088551222 1105 66-67 -0.16170770325168604 1105 68-69 -0.011544318181421431 1105 70-71 -0.27250064125729523 1105 72-73 -0.18065618376892445 1105 74-75 -0.11766825320309948 1105 76-77 -0.23606848177497142 1105 78-79 -0.09057958556894619 1105 80-81 -0.32009923364376647 1105 82-83 -0.082323275203116 1105 84-85 -0.035169589902217524 1105 86-87 -0.19800526234539007 1105 88-89 -0.1770943936339009 1105 90-91 -0.026614519994744512 1105 92-93 -0.11545930116695047 1105 94-95 -0.16061918103568473 1105 96-97 -0.005016134372198167 1105 98-99 -0.1713590694856535 1105 100 -0.14363445079340664 1106 1 1.5394053118035238 1106 2 -0.6385275669479427 1106 3 -0.11410244867135866 1106 4 -0.16765363268687672 1106 5 -1.0407960153952267 1106 6 -0.571301311993949 1106 7 -0.10794385556582853 1106 8 -0.02702340547703841 1106 9 -0.06188831077984247 1106 10-11 -0.0639534854778816 1106 12-13 0.02110643335365836 1106 14-15 0.016024537797306948 1106 16-17 -0.18388052303548363 1106 18-19 0.04174785220374844 1106 20-21 0.024058484782536027 1106 22-23 -0.040750781442866923 1106 24-25 -0.18419434191553563 1106 26-27 -0.060919663136452584 1106 28-29 0.025511248528474084 1106 30-31 0.03206388636374413 1106 32-33 -0.06875400743753346 1106 34-35 -0.15826386763587408 1106 36-37 -0.3242333064298535 1106 38-39 -0.06681695361239548 1106 40-41 -0.1515083180973491 1106 42-43 0.05482206129876488 1106 44-45 -0.03063573459763802 1106 46-47 -0.2045908056100032 1106 48-49 -0.20461305735889823 1106 50-51 -0.12472840378998029 1106 52-53 -0.12587833179770058 1106 54-55 -0.20474583076133257 1106 56-57 -0.13391775532785744 1106 58-59 -0.1264530554576524 1106 60-61 -0.12773597037104167 1106 62-63 -0.23915244697774796 1106 64-65 -0.4513942430143132 1106 66-67 -0.37903221162097367 1106 68-69 -0.23949918792733627 1106 70-71 -0.5857344097149451 1106 72-73 -0.35321343841572883 1106 74-75 -0.526131004949427 1106 76-77 -0.553363552801386 1106 78-79 -0.340103260465753 1106 80-81 -0.42443261409655264 1106 82-83 -0.40619594235936063 1106 84-85 -0.43185494181144435 1106 86-87 -0.2606181458537975 1106 88-89 -0.4142941159082483 1106 90-91 -0.46160076095113567 1106 92-93 -0.3537678081321758 1106 94-95 -0.3297602755499156 1106 96-97 -0.19935103019963663 1106 98-99 -0.5855677672764372 1106 100 -0.5986412043076257 1107 1 1.4007977663191866 1107 2 -0.7660154881321386 1107 3 -0.155986172503507 1107 4 -0.199222403510662 1107 5 -1.279590967657228 1107 6 -0.2856336738907821 1107 7 -0.3564228518958217 1107 8 -0.4973887612292103 1107 9 -0.3923376555558349 1107 10-11 -0.16059684000089192 1107 12-13 -0.38558959798420744 1107 14-15 -0.43249632476367594 1107 16-17 -0.27326201069776346 1107 18-19 -0.2753305379405475 1107 20-21 -0.2954595754232088 1107 22-23 -0.35771546803506027 1107 24-25 -0.27188485367481974 1107 26-27 -0.4348865643239819 1107 28-29 -0.21140788667145927 1107 30-31 -0.3737790767858087 1107 32-33 -0.2804612750187232 1107 34-35 -0.24317418177254524 1107 36-37 -0.2620712829497762 1107 38-39 -0.3237409168400873 1107 40-41 -0.4865402075704921 1107 42-43 -0.24181476255868972 1107 44-45 -0.251548908232877 1107 46-47 -0.48670681122171544 1107 48-49 -0.2958493706582459 1107 50-51 -0.2707634151545051 1107 52-53 -0.3628995086469544 1107 54-55 -0.24000676000506616 1107 56-57 -0.1888173288520747 1107 58-59 -0.44327896540676903 1107 60-61 -0.30899925765151437 1107 62-63 -0.3447352330762854 1107 64-65 -0.28329432849606206 1107 66-67 -0.31166839640614796 1107 68-69 -0.7683274115979657 1107 70-71 -0.46987632107748567 1107 72-73 -0.4741112183392957 1107 74-75 -0.5245887183068589 1107 76-77 -0.6807412690043151 1107 78-79 -0.658154253301845 1107 80-81 -0.641751006200046 1107 82-83 -0.3886750996236188 1107 84-85 -0.3392594337431589 1107 86-87 -0.35606790462734494 1107 88-89 -0.2168143697178948 1107 90-91 -0.18187078064809015 1107 92-93 -0.24417332747751885 1107 94-95 -0.25623314471619096 1107 96-97 -0.3223106345346025 1107 98-99 -0.4672431862011379 1107 100 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26-27 0.13123279659645704 2223 28-29 0.08444214238189574 2223 30-31 0.14928000357986093 2223 32-33 0.2628496954415027 2223 34-35 0.1784792462228566 2223 36-37 0.18741010688221138 2223 38-39 0.1378744173483426 2223 40-41 0.23335956200295271 2223 42-43 0.3536762993663487 2223 44-45 0.39511070366369694 2223 46-47 0.30788334452802957 2223 48-49 0.31458266326709605 2223 50-51 0.2798862725767819 2223 52-53 0.4581946604993661 2223 54-55 0.3989078104370094 2223 56-57 0.43873618323787866 2223 58-59 0.5832734295966091 2223 60-61 0.4098533550609531 2223 62-63 0.41838557408828336 2223 64-65 0.23794438374982718 2223 66-67 0.35602119566127755 2223 68-69 0.5018777638191025 2223 70-71 0.4564223916790411 2223 72-73 0.41213069522398627 2223 74-75 0.3713073179954449 2223 76-77 0.5264657689826464 2223 78-79 0.5223439422675042 2223 80-81 0.33391150062420394 2223 82-83 0.3469340557050842 2223 84-85 0.19903643759006684 2223 86-87 0.3931370514055672 2223 88-89 0.4939134669119696 2223 90-91 0.34514705354399666 2223 92-93 0.3192738237890751 2223 94-95 0.47991658359059386 2223 96-97 0.5297885010853065 2223 98-99 0.5890160032548906 2223 100 0.6742974405022935 2224 1 1.1016194711740805 2224 2 -0.8095111384054476 2224 3 0.017615710297985032 2224 4 -0.36416153061657575 2224 5 -1.1198355908802995 2224 6 0.6057057690415668 2224 7 0.3608150053731336 2224 8 0.2414174743382489 2224 9 0.3946841671196495 2224 10-11 0.16935949783510296 2224 12-13 0.1659631344899779 2224 14-15 0.21010417678215276 2224 16-17 0.20691509524829144 2224 18-19 0.1582618454949909 2224 20-21 0.2303380091900209 2224 22-23 0.15101898556387283 2224 24-25 0.22502744823042065 2224 26-27 0.41792499580298426 2224 28-29 0.3360508712718939 2224 30-31 0.3155517255387892 2224 32-33 0.3331745668844093 2224 34-35 0.3176358250884661 2224 36-37 0.20879713951528345 2224 38-39 0.22697657951763262 2224 40-41 0.18173242746566842 2224 42-43 0.4213387068438408 2224 44-45 0.39032782439909397 2224 46-47 0.3034549269617912 2224 48-49 0.25353051926808945 2224 50-51 0.24929170897421216 2224 52-53 0.22286363187317448 2224 54-55 0.23704732430197595 2224 56-57 0.5664031191636312 2224 58-59 0.5412412390138925 2224 60-61 0.40431000717257604 2224 62-63 0.4211078211024315 2224 64-65 0.3392679427900518 2224 66-67 0.3544440058599889 2224 68-69 0.3730332901264717 2224 70-71 0.47681923814675997 2224 72-73 0.4959034575021093 2224 74-75 0.2706957421142775 2224 76-77 0.6350894502250739 2224 78-79 0.5272346193128854 2224 80-81 0.3758992069698692 2224 82-83 0.30842106254200274 2224 84-85 0.14474142566169945 2224 86-87 0.3528799937106868 2224 88-89 0.5513251998295488 2224 90-91 0.4911462024593405 2224 92-93 0.5101469763987296 2224 94-95 0.6398569511763199 2224 96-97 0.3230839027803256 2224 98-99 0.41365584569125247 2224 100 0.6727779154527269 2225 1 1.6791907284619043 2225 2 1.8297293842080329 2225 3 0.812860035701549 2225 4 1.0443309384182147 2225 5 3.2280971887585643 2225 6 -0.1689081628581306 2225 7 0.2014432376160542 2225 8 0.44592614768424 2225 9 0.28806260391048255 2225 10-11 0.21774440554411711 2225 12-13 0.2929317473206652 2225 14-15 0.1479685679498033 2225 16-17 0.108163480734909 2225 18-19 0.087724977985431 2225 20-21 0.25305794322010655 2225 22-23 0.203684283431933 2225 24-25 0.2140947483523803 2225 26-27 0.1537848174475016 2225 28-29 0.07898689280216331 2225 30-31 0.12417142594685515 2225 32-33 0.07402458566679115 2225 34-35 0.13531931356946103 2225 36-37 0.15981048787453034 2225 38-39 0.24608540672481638 2225 40-41 0.0968344312633107 2225 42-43 0.34101087700712185 2225 44-45 0.168637448185315 2225 46-47 0.12734528670882383 2225 48-49 0.1529546976029934 2225 50-51 0.09622426219525693 2225 52-53 0.06819324989603359 2225 54-55 0.08064439579339222 2225 56-57 0.2263335342801014 2225 58-59 0.1757803758474452 2225 60-61 0.21623720869985874 2225 62-63 0.12329989287687937 2225 64-65 0.21389100859015997 2225 66-67 0.2909180501452582 2225 68-69 0.18926339494761635 2225 70-71 0.21218151951205755 2225 72-73 0.29347242488250913 2225 74-75 0.3989582169638979 2225 76-77 0.4158755188399681 2225 78-79 0.3985688377340324 2225 80-81 0.15322243383537426 2225 82-83 0.03574808447880429 2225 84-85 0.00963560106346506 2225 86-87 0.018009013270102514 2225 88-89 0.13683265875273065 2225 90-91 0.2329629995735374 2225 92-93 0.18425101795668297 2225 94-95 0.3201823367298289 2225 96-97 0.32704524965755866 2225 98-99 0.36753736742479504 2225 100 0.4779807652296526 2226 1 1.6056764558704533 2226 2 1.1554636030561927 2226 3 0.9909143755513004 2226 4 1.2675266051675909 2226 5 3.0380456363799766 2226 6 -0.5267524129069621 2226 7 -0.09676140394577004 2226 8 0.2540928310686965 2226 9 0.24918384834742113 2226 10-11 0.10729844975298874 2226 12-13 0.14301377446683716 2226 14-15 0.03421743949911615 2226 16-17 0.16868803212626915 2226 18-19 0.03238074113485112 2226 20-21 0.12630752483546104 2226 22-23 0.11011724422256464 2226 24-25 0.12732184014359405 2226 26-27 0.1965940129347601 2226 28-29 0.11599017652766719 2226 30-31 0.09937454706987126 2226 32-33 0.038091020396151976 2226 34-35 0.07940095268697434 2226 36-37 0.11015601815396536 2226 38-39 0.1710800614321002 2226 40-41 0.18936375778282866 2226 42-43 0.3444585908056297 2226 44-45 0.1984711105951078 2226 46-47 0.17280568138939856 2226 48-49 0.15375385185890167 2226 50-51 0.05101826385295993 2226 52-53 0.24586484039117806 2226 54-55 0.421225660080502 2226 56-57 0.3376994083476461 2226 58-59 0.3623482491864465 2226 60-61 0.4284717258285511 2226 62-63 0.37368693166390443 2226 64-65 0.23829523942233521 2226 66-67 0.39735503640323344 2226 68-69 0.46878689839765286 2226 70-71 0.3619978658131089 2226 72-73 0.5129270466469578 2226 74-75 0.4425843843494235 2226 76-77 0.4213273874487129 2226 78-79 0.31550232909445697 2226 80-81 0.2698831172937375 2226 82-83 0.25767770875091855 2226 84-85 0.14703140085124033 2226 86-87 0.3465976844171337 2226 88-89 0.40833921300546905 2226 90-91 0.4381826449100643 2226 92-93 0.534961281974546 2226 94-95 0.608712328441328 2226 96-97 0.6087438253020281 2226 98-99 0.5335212444381838 2226 100 0.556942616997425 2227 1 1.278606464839628 2227 2 1.7641479667955515 2227 3 0.6446767081660507 2227 4 0.0894209629799505 2227 5 3.3378343887206157 2227 6 0.25994318065831123 2227 7 0.3282128949113101 2227 8 0.4181782375401397 2227 9 0.18924773404552298 2227 10-11 0.21466694120080376 2227 12-13 0.2644136475346528 2227 14-15 0.36015153180294135 2227 16-17 0.34670155669708436 2227 18-19 0.2592651200850895 2227 20-21 0.32468953298639747 2227 22-23 0.4125308569115873 2227 24-25 0.4673145231855571 2227 26-27 0.32107343366246965 2227 28-29 0.3058337667150681 2227 30-31 0.3246440812497724 2227 32-33 0.26054883642841986 2227 34-35 0.36697707265361146 2227 36-37 0.2966512477071248 2227 38-39 0.25809285430712237 2227 40-41 0.40170896084809016 2227 42-43 0.5118625262275742 2227 44-45 0.46664417894440646 2227 46-47 0.24887778620809797 2227 48-49 0.3820390086620975 2227 50-51 0.5108313798280619 2227 52-53 0.5172862840610932 2227 54-55 0.4761700485768827 2227 56-57 0.6198615694466127 2227 58-59 0.48022995049719697 2227 60-61 0.46309254218078166 2227 62-63 0.4268086370658679 2227 64-65 0.3256653568608243 2227 66-67 0.46220256043904584 2227 68-69 0.4875988892921015 2227 70-71 0.6750977776949156 2227 72-73 0.5794697315247177 2227 74-75 0.44401991119662654 2227 76-77 0.623308211249018 2227 78-79 0.5999533343238497 2227 80-81 0.6090172379212575 2227 82-83 0.3465476058938961 2227 84-85 0.44131997747997787 2227 86-87 0.38010817205697833 2227 88-89 0.3834363134969365 2227 90-91 0.38053963640771116 2227 92-93 0.45464094869890914 2227 94-95 0.5614671369153683 2227 96-97 0.42688725866001676 2227 98-99 0.6363605650887862 2227 100 0.7238695733002416 2228 1 1.3466776919884094 2228 2 1.8769349525097816 2228 3 -0.19580985359090874 2228 4 0.13391692058163862 2228 5 3.145189349175837 2228 6 0.12155988785886152 2228 7 0.1751898882151437 2228 8 0.21310894851868056 2228 9 0.15303196966181076 2228 10-11 0.12258229714260693 2228 12-13 0.157847675568334 2228 14-15 0.1630446135029615 2228 16-17 0.22344533794599641 2228 18-19 0.04941394796413334 2228 20-21 0.05124872991125784 2228 22-23 0.11993803625753685 2228 24-25 0.22407243311112524 2228 26-27 0.08752084886777567 2228 28-29 0.20191381046839751 2228 30-31 0.12237895150902034 2228 32-33 0.16334573904495642 2228 34-35 0.26688456230680657 2228 36-37 0.25453948408884486 2228 38-39 0.1099880557934867 2228 40-41 0.07850748223408033 2228 42-43 0.2873559632293876 2228 44-45 0.19785523700160468 2228 46-47 0.2862953446848522 2228 48-49 0.15612418412145246 2228 50-51 0.3058155864823391 2228 52-53 0.249498810259432 2228 54-55 0.15492407125775998 2228 56-57 0.2539169651077344 2228 58-59 0.3627102944727767 2228 60-61 0.17940990309525517 2228 62-63 0.21413738625615508 2228 64-65 0.17275014960791424 2228 66-67 0.22811889772750504 2228 68-69 0.23937353964694807 2228 70-71 0.3805660216274305 2228 72-73 0.33536742775203265 2228 74-75 0.3752353814349263 2228 76-77 0.2863274748640734 2228 78-79 0.46204657324620513 2228 80-81 0.4144979911145228 2228 82-83 0.26557058737760286 2228 84-85 0.40181043100694325 2228 86-87 0.28645259758130237 2228 88-89 0.37272206703282507 2228 90-91 0.3749811771090279 2228 92-93 0.25641401519387585 2228 94-95 0.5723905175317654 2228 96-97 0.3509301870811896 2228 98-99 0.6191842510992345 2228 100 0.6145255434176491 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 20.0 13 33.0 14 150.0 15 494.0 16 1723.0 17 4396.0 18 7329.0 19 9408.0 20 11257.0 21 12709.0 22 14747.0 23 16758.0 24 18936.0 25 21642.0 26 24649.0 27 27611.0 28 31337.0 29 35665.0 30 40808.0 31 47199.0 32 54975.0 33 65842.0 34 80765.0 35 104167.0 36 146315.0 37 243138.0 38 596908.0 39 1138439.0 40 1452.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.989955031865204 19.330411760305658 18.811117892052767 35.86851531577637 2 18.43723878037941 53.042417897744365 11.600561715072422 16.9197816068038 3 42.611493896239516 14.687809116258704 23.335506926197763 19.36519006130402 4 16.896029646888422 16.37056145752269 22.231070440719815 44.502338454869076 5 17.79657853043616 35.277810903220264 26.128241495712203 20.797369070631376 6 17.134931546323447 27.825021303988983 31.060871595031742 23.979175554655832 7 20.348055166004382 26.509411632938523 36.459380333926205 16.68315286713089 8 20.789213566554167 27.833430534859705 34.25322586432939 17.12413003425674 9 20.759527531713132 28.07925555108916 27.906213877913196 23.25500303928452 10-11 19.530511190620228 30.370426619855284 28.80014774148719 21.298914448037294 12-13 24.28246968961601 26.553342614901084 27.368258705638137 21.795928989844764 14-15 21.808470342781273 27.35513740574503 28.682617866063424 22.153774385410273 16-17 20.89481828268282 29.701475928757866 28.35701389661648 21.046691891942835 18-19 21.334762419292225 29.458786250762543 29.946231667786083 19.26021966215915 20-21 22.730459140380873 28.89136252375517 29.91157983712915 18.46659849873481 22-23 21.99755117397575 30.472334174135597 28.653203681358292 18.876910970530357 24-25 22.163234770139837 28.340358544956583 26.70617676130797 22.79022992359561 26-27 20.161475356060247 26.412361134419744 30.052361960844152 23.373801548675853 28-29 19.680404630384942 28.142312948792135 30.64272661550671 21.53455580531621 30-31 20.143388600144096 30.097033354932552 28.229338384227436 21.53023966069592 32-33 20.51118337089094 30.15967027115782 28.817274300049334 20.511872057901904 34-35 21.375866159841355 29.282699856064415 29.953807224906694 19.387626759187537 36-37 21.75361098535525 28.046977878285805 28.97175767061405 21.22765346574489 38-39 21.553412592246364 29.25880127161749 28.89808918467252 20.289696951463625 40-41 20.958940307093172 29.691243085401563 30.00374795611339 19.346068651391874 42-43 20.39891702017801 30.044951383166186 29.42176134101305 20.134370255642757 44-45 20.552232741412887 30.25202682399661 28.84576419429238 20.34997624029812 46-47 19.822963877069707 28.11579824747251 28.929698270870237 23.131539604587548 48-49 20.820280748747905 28.129915391160697 30.386235681424207 20.66356817866719 50-51 21.789422709925393 30.63113090019004 28.252025374129218 19.32742101575535 52-53 20.293409655293402 30.378974633894913 28.282223065572147 21.045392645239538 54-55 22.326458318945054 28.44322172016569 28.20876754214474 21.021552418744516 56-57 21.709808214808117 28.60483115254182 29.245148275093303 20.440212357556764 58-59 21.159222902202632 27.95118859218316 29.176077748206495 21.713510757407708 60-61 19.406633552849655 30.610375231729964 29.522157362701414 20.460833852718967 62-63 20.507895941236978 31.236316345164784 27.402273849820784 20.853513863777454 64-65 19.65116833308172 30.184679106437372 29.003632470074216 21.160520090406695 66-67 20.172530594920463 30.289759622860498 29.513410004737437 20.024299777481602 68-69 20.869300616592355 29.918720434032302 29.326540221315007 19.885438728060333 70-71 19.65349619210453 31.070875680033115 29.165423707434158 20.110204420428197 72-73 19.92054364227712 30.783656949776233 28.62830220890922 20.66749719903743 74-75 19.93440799920837 29.246815638124694 30.540786038356966 20.277990324309965 76-77 19.487160155614266 30.688001948621775 28.557059349959204 21.267778545804756 78-79 19.569222649973376 29.41726567333835 28.558219243872408 22.455292432815863 80-81 20.834805857037793 29.41357491058836 28.45116966409061 21.300449568283238 82-83 20.205206981256477 28.16720813172625 29.854618171985447 21.772966715031828 84-85 21.152459718153025 28.74168183892481 28.40536697412313 21.700491468799036 86-87 21.904578427495576 28.06125907209212 29.852280260816645 20.181882239595662 88-89 21.38382230715223 27.250167006600158 30.652298964105995 20.713711722141614 90-91 21.080491925507264 27.896264162938998 30.329141645882213 20.69410226567153 92-93 20.39461403261404 28.23727297342067 31.547410844935598 19.820702149029696 94-95 20.663038856808562 28.624369443609766 30.320569304929947 20.39202239465173 96-97 20.20598628497941 28.43344003149112 30.98687398803787 20.373699695491602 98-99 22.14344408024581 28.90047856497925 29.62548475410068 19.330592600674258 100 20.954607189239955 29.921747032212064 29.833232627960765 19.290413150587217 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 7.5 2 13.5 3 20.5 4 24.0 5 27.5 6 33.5 7 37.5 8 49.5 9 60.0 10 73.0 11 84.5 12 104.0 13 135.5 14 161.0 15 186.5 16 240.5 17 319.0 18 486.0 19 759.5 20 1162.5 21 1819.0 22 2877.0 23 4454.0 24 6885.5 25 10169.0 26 14378.0 27 19458.0 28 25338.0 29 32267.5 30 40002.0 31 48632.0 32 58715.5 33 70035.0 34 82878.5 35 97166.0 36 110817.0 37 121178.0 38 128093.5 39 133695.5 40 137055.0 41 137304.0 42 135762.5 43 132759.5 44 130204.0 45 126772.5 46 117653.5 47 106385.0 48 98794.0 49 94133.5 50 89890.0 51 83678.0 52 73015.5 53 60235.0 54 49621.0 55 40509.0 56 31378.5 57 23089.5 58 16374.0 59 11633.0 60 8942.5 61 7371.0 62 5960.0 63 4507.0 64 3332.5 65 2835.5 66 2932.0 67 2646.0 68 1923.5 69 1789.0 70 2176.5 71 2024.5 72 1261.5 73 628.5 74 308.0 75 173.5 76 112.5 77 87.5 78 81.0 79 64.0 80 47.0 81 34.0 82 29.5 83 31.5 84 27.5 85 21.5 86 23.5 87 23.5 88 15.0 89 13.0 90 14.5 91 11.5 92 14.0 93 14.5 94 14.5 95 14.0 96 12.5 97 20.0 98 38.5 99 83.5 100 89.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.326146582318215 2 0.0064881565769790775 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 3.4434350548212983E-4 30-31 0.010348428506857691 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.88043777296019E-4 40-41 0.0013048806523533341 42-43 1.2686339675657414E-4 44-45 0.0 46-47 0.002084184375286575 48-49 1.8123342393796308E-5 50-51 0.0 52-53 0.001232387282778149 54-55 0.01999004666035733 56-57 0.011526445762454452 58-59 0.013719370192103805 60-61 1.6311008154416677E-4 62-63 0.012160762746237322 64-65 2.3560345111935197E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.4498673915037046E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2758873.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.2729708208435 #Duplication Level Percentage of deduplicated Percentage of total 1 85.7499408574828 62.83152914339393 2 8.712799098247153 12.768253481874703 3 2.465474142243539 5.419578446524649 4 1.0596746376543564 3.105820352177423 5 0.5818646728346577 2.131747659714676 6 0.3364812843350304 1.4792989997304375 7 0.23715914703942728 1.216414868264126 8 0.16692770977927449 0.978503136627761 9 0.12407035590562736 0.8181903211204232 >10 0.5309462180944636 6.465596670472501 >50 0.024064427794474277 1.1996807241770846 >100 0.010093045957988152 1.3206852701053227 >500 4.5396236814782226E-4 0.22293716623207385 >1k 5.04402631211354E-5 0.04176375958493803 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.6246684787592616E-5 0.0 16-17 0.0 0.0 0.0 3.6246684787592616E-5 0.0 18-19 1.8123342393796308E-5 0.0 0.0 3.6246684787592616E-5 0.0 20-21 3.6246684787592616E-5 0.0 0.0 3.6246684787592616E-5 0.0 22-23 3.6246684787592616E-5 0.0 0.0 3.6246684787592616E-5 0.0 24-25 3.6246684787592616E-5 0.0 0.0 3.6246684787592616E-5 0.0 26-27 3.6246684787592616E-5 0.0 0.0 3.6246684787592616E-5 0.0 28-29 3.6246684787592616E-5 0.0 0.0 3.6246684787592616E-5 0.0 30-31 3.6246684787592616E-5 0.0 0.0 3.6246684787592616E-5 0.0 32-33 3.6246684787592616E-5 0.0 0.0 7.249336957518523E-5 0.0 34-35 3.6246684787592616E-5 0.0 0.0 7.249336957518523E-5 0.0 36-37 3.6246684787592616E-5 0.0 0.0 7.249336957518523E-5 0.0 38-39 3.6246684787592616E-5 0.0 0.0 7.249336957518523E-5 0.0 40-41 3.6246684787592616E-5 0.0 0.0 7.249336957518523E-5 0.0 42-43 3.6246684787592616E-5 0.0 0.0 7.249336957518523E-5 0.0 44-45 3.6246684787592616E-5 0.0 0.0 7.249336957518523E-5 0.0 46-47 7.249336957518523E-5 0.0 0.0 7.249336957518523E-5 0.0 48-49 9.061671196898153E-5 0.0 0.0 7.249336957518523E-5 0.0 50-51 1.0874005436277785E-4 0.0 0.0 7.249336957518523E-5 0.0 52-53 1.0874005436277785E-4 0.0 0.0 7.249336957518523E-5 0.0 54-55 1.2686339675657416E-4 0.0 0.0 7.249336957518523E-5 0.0 56-57 1.4498673915037046E-4 0.0 0.0 7.249336957518523E-5 0.0 58-59 2.35603451119352E-4 0.0 0.0 7.249336957518523E-5 0.0 60-61 4.168368750573151E-4 0.0 0.0 7.249336957518523E-5 0.0 62-63 4.71206902238704E-4 0.0 0.0 7.249336957518523E-5 0.0 64-65 5.25576929420093E-4 0.0 0.0 7.249336957518523E-5 0.0 66-67 5.618236142076855E-4 0.0 0.0 7.249336957518523E-5 0.0 68-69 6.161936413890745E-4 0.0 0.0 7.249336957518523E-5 0.0 70-71 6.705636685704634E-4 0.0 0.0 7.249336957518523E-5 0.0 72-73 7.430570381456486E-4 0.0 0.0 7.249336957518523E-5 0.0 74-75 9.061671196898154E-4 0.0 0.0 7.249336957518523E-5 0.0 76-77 9.786604892650006E-4 0.0 0.0 7.249336957518523E-5 0.0 78-79 0.001051153858840186 0.0 0.0 7.249336957518523E-5 0.0 80-81 0.001359250679534723 0.0 0.0 7.249336957518523E-5 0.0 82-83 0.0016492241578354638 0.0 0.0 7.249336957518523E-5 0.0 84-85 0.0017398408698044456 0.0 0.0 7.249336957518523E-5 0.0 86-87 0.0017398408698044456 3.6246684787592616E-5 0.0 7.249336957518523E-5 0.0 88 0.0017398408698044456 3.6246684787592616E-5 0.0 7.249336957518523E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 10680 0.0 80.252045 8 CACCGGC 10775 0.0 76.843636 9 CTTCACC 12130 0.0 73.71583 6 CGTAGTG 2365 0.0 69.663055 4 CCTTCAC 13735 0.0 69.13433 5 GGGCCCG 750 0.0 68.84272 8 CGGGCCC 755 0.0 68.38681 7 GTGTGGC 4730 0.0 68.373 7 GGCGCGC 285 0.0 65.8782 6 TTCACCG 13610 0.0 64.59609 7 CGGGGCA 1065 0.0 62.58429 94 CAGCGTA 995 0.0 61.162693 1 TGTGGCA 5385 0.0 60.579502 8 CAGCTGC 70 4.965841E-10 60.47889 1 AGGCTGC 55 1.3003955E-8 59.74171 2 ACCTTCA 5685 0.0 59.116558 4 TGTGTGG 5140 0.0 58.99239 6 GCTGCCT 180 0.0 58.79892 1 AACGGGC 890 0.0 58.54092 5 GGCTGCT 120 0.0 57.32894 1 >>END_MODULE