##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1SN_27A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2607809
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.808527771780833	12.0	12.0	12.0	2.0	32.0
2	14.673520184952196	12.0	12.0	12.0	12.0	22.0
3	13.718929568844958	12.0	12.0	12.0	12.0	22.0
4	13.808905483492081	12.0	12.0	12.0	12.0	22.0
5	22.431925804382146	22.0	22.0	22.0	22.0	27.0
6	26.853857395231017	27.0	22.0	32.0	22.0	32.0
7	34.9700806309051	37.0	37.0	37.0	27.0	41.0
8	37.557984116168015	41.0	37.0	41.0	27.0	41.0
9	38.49933028070691	41.0	37.0	41.0	32.0	41.0
10-11	38.86621010204352	41.0	41.0	41.0	37.0	41.0
12-13	39.04816092742988	41.0	41.0	41.0	37.0	41.0
14-15	39.002059199887725	41.0	41.0	41.0	37.0	41.0
16-17	38.911673746045054	41.0	41.0	41.0	37.0	41.0
18-19	38.85107555806426	41.0	41.0	41.0	37.0	41.0
20-21	38.866829204132664	41.0	41.0	41.0	37.0	41.0
22-23	38.85372088216583	41.0	41.0	41.0	34.5	41.0
24-25	38.87790075883625	41.0	41.0	41.0	37.0	41.0
26-27	38.84202006358595	41.0	41.0	41.0	37.0	41.0
28-29	38.786439305946104	41.0	41.0	41.0	34.5	41.0
30-31	38.666672674264106	41.0	41.0	41.0	32.0	41.0
32-33	38.7117170007466	41.0	41.0	41.0	32.0	41.0
34-35	38.68172707433712	41.0	41.0	41.0	32.0	41.0
36-37	38.60858866581103	41.0	41.0	41.0	32.0	41.0
38-39	38.52720482980157	41.0	41.0	41.0	32.0	41.0
40-41	38.56080219065123	41.0	41.0	41.0	32.0	41.0
42-43	38.30135105753527	41.0	41.0	41.0	32.0	41.0
44-45	38.4110887722222	41.0	41.0	41.0	32.0	41.0
46-47	38.41416108311613	41.0	41.0	41.0	32.0	41.0
48-49	38.37728779216576	41.0	41.0	41.0	32.0	41.0
50-51	38.27052613899254	41.0	41.0	41.0	32.0	41.0
52-53	38.23217708812264	41.0	41.0	41.0	32.0	41.0
54-55	38.11078054412727	41.0	37.0	41.0	32.0	41.0
56-57	37.88590709672373	41.0	37.0	41.0	29.5	41.0
58-59	37.88821535626267	41.0	37.0	41.0	29.5	41.0
60-61	37.90241961738762	41.0	37.0	41.0	29.5	41.0
62-63	37.899231883930156	41.0	37.0	41.0	29.5	41.0
64-65	37.88113393273817	41.0	37.0	41.0	29.5	41.0
66-67	37.75009155195032	41.0	37.0	41.0	27.0	41.0
68-69	37.76014386022903	41.0	37.0	41.0	27.0	41.0
70-71	37.7242253938076	41.0	37.0	41.0	27.0	41.0
72-73	37.58928050328839	41.0	37.0	41.0	27.0	41.0
74-75	37.51487359695438	41.0	37.0	41.0	27.0	41.0
76-77	36.4586083566703	39.0	34.5	41.0	24.5	41.0
78-79	36.966388067531014	41.0	37.0	41.0	27.0	41.0
80-81	37.59706155627195	41.0	37.0	41.0	27.0	41.0
82-83	37.80139918222538	41.0	37.0	41.0	27.0	41.0
84-85	37.700758567824565	41.0	37.0	41.0	27.0	41.0
86-87	37.573164292323554	41.0	37.0	41.0	27.0	41.0
88-89	37.47831187023283	41.0	37.0	41.0	27.0	41.0
90-91	37.317869713617824	41.0	37.0	41.0	27.0	41.0
92-93	37.21440814875629	41.0	37.0	41.0	27.0	41.0
94-95	37.065090464830824	41.0	37.0	41.0	27.0	41.0
96-97	37.025612496927494	41.0	37.0	41.0	27.0	41.0
98-99	36.89591914131748	41.0	37.0	41.0	27.0	41.0
100	36.7733959810707	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.5830037887842074
1101	2	-0.4906994053717071
1101	3	-0.08238108385033271
1101	4	0.08620034235266871
1101	5	-1.1203950225997623
1101	6	-0.9144675814195473
1101	7	-0.30566658751408937
1101	8	-0.3010997062661147
1101	9	-0.16173597631522796
1101	10-11	-0.21521191435081022
1101	12-13	-0.0556590198319995
1101	14-15	-0.18092838995220006
1101	16-17	-0.18662251896197546
1101	18-19	-0.013083063871597744
1101	20-21	-0.07788599890965742
1101	22-23	0.006141512975858632
1101	24-25	-0.09049599636525585
1101	26-27	-0.04923543808912001
1101	28-29	-0.04133618293982977
1101	30-31	-0.024681341360469844
1101	32-33	-0.325538959932949
1101	34-35	-0.13541242044437496
1101	36-37	0.08549389340335978
1101	38-39	-0.012373098004374583
1101	40-41	-0.12015684581203345
1101	42-43	-0.03401285574297219
1101	44-45	-0.10396361389741315
1101	46-47	-0.13879797288026197
1101	48-49	-0.07521584226469002
1101	50-51	-0.20446539587314305
1101	52-53	-0.22670348385463512
1101	54-55	-0.10367877630086042
1101	56-57	0.10703571223533004
1101	58-59	-0.057137449606180724
1101	60-61	-0.07273733856408171
1101	62-63	-0.14983969029425026
1101	64-65	-0.25677658130626924
1101	66-67	-0.2223868993099387
1101	68-69	-0.14193447573254758
1101	70-71	-0.2698205751307867
1101	72-73	-0.10101054570858281
1101	74-75	0.009985273512064907
1101	76-77	-0.40107833943361015
1101	78-79	-0.35489194054942885
1101	80-81	-0.33327323009024923
1101	82-83	-0.36594070142837865
1101	84-85	-0.3049410453634849
1101	86-87	-0.3303668957840955
1101	88-89	-0.42131002415626995
1101	90-91	-0.316925266008667
1101	92-93	-0.24116617307438304
1101	94-95	-0.24804359989627045
1101	96-97	-0.26510864484470176
1101	98-99	-0.5295611569573566
1101	100	-0.4358645522318625
1102	1	1.6397770880881346
1102	2	0.9320850015249373
1102	3	-0.07636557721099635
1102	4	-0.009194500523625138
1102	5	-0.8342721185463802
1102	6	-0.24723782414453055
1102	7	-0.30207359034123016
1102	8	-0.34547243445243225
1102	9	-0.4523261880572633
1102	10-11	-0.4038214174291923
1102	12-13	-0.35993860958297574
1102	14-15	-0.2675554778402187
1102	16-17	-0.3980107405146427
1102	18-19	-0.2477826298449699
1102	20-21	-0.18433050740151913
1102	22-23	-0.31157716420048587
1102	24-25	-0.33098618668090296
1102	26-27	-0.26977756370086325
1102	28-29	-0.45285106669691544
1102	30-31	-0.32783149485306495
1102	32-33	-0.272595959821075
1102	34-35	0.006139798324760193
1102	36-37	-0.3792855759426814
1102	38-39	-0.3646679023890016
1102	40-41	-0.18759138629179972
1102	42-43	-0.2732794664102798
1102	44-45	-0.02819259565084309
1102	46-47	-0.5048397031466507
1102	48-49	-0.4133985240524467
1102	50-51	-0.28835028495839055
1102	52-53	-0.23431952293030633
1102	54-55	-0.34576665484166824
1102	56-57	-0.27147883211255674
1102	58-59	-0.18120696591963537
1102	60-61	-0.26135945097868074
1102	62-63	-0.47146195250397227
1102	64-65	-0.337954729421412
1102	66-67	-0.22212399939014205
1102	68-69	-0.200988547979307
1102	70-71	-0.576573585728319
1102	72-73	-0.353887275700032
1102	74-75	-0.3262238101399717
1102	76-77	-0.4228998860398008
1102	78-79	-0.3259506697179546
1102	80-81	-0.4272045367019004
1102	82-83	-0.4096858070149807
1102	84-85	-0.46376103903037347
1102	86-87	-0.4268381777838428
1102	88-89	-0.47589147952016475
1102	90-91	-0.546378968887204
1102	92-93	-0.4579376331217375
1102	94-95	-0.2912663186966995
1102	96-97	-0.29742561511510957
1102	98-99	-0.48037327405542385
1102	100	-0.8193918291651201
1103	1	1.2442421971056827
1103	2	0.6808230910710584
1103	3	0.4033409121263123
1103	4	0.991131405597848
1103	5	2.859512734625106
1103	6	-0.2632993364855416
1103	7	-0.49235124479292836
1103	8	-0.23460444106813583
1103	9	-0.19223202655809501
1103	10-11	-0.20854295796986122
1103	12-13	-0.36407273849153654
1103	14-15	-0.2308111238710424
1103	16-17	-0.27869650944330004
1103	18-19	-0.2833907033397267
1103	20-21	-0.38543273361354835
1103	22-23	-0.2482267712103905
1103	24-25	-0.13600406760807715
1103	26-27	-0.20225001048498115
1103	28-29	-0.2826423013834827
1103	30-31	-0.2452323447459719
1103	32-33	-0.3485226211860706
1103	34-35	-0.3322545773552221
1103	36-37	-0.24622044838556434
1103	38-39	-0.09566538003174685
1103	40-41	-0.43768101047520247
1103	42-43	-0.16540385716586314
1103	44-45	-0.4785718274296187
1103	46-47	-0.5549909957937587
1103	48-49	-0.43446367112490236
1103	50-51	-0.3640453968544506
1103	52-53	-0.10346811758212482
1103	54-55	-0.1081093734256342
1103	56-57	-0.16325867960020446
1103	58-59	-0.4318967842806387
1103	60-61	-0.2682503163374932
1103	62-63	-0.2992867242965076
1103	64-65	-0.3411512439822886
1103	66-67	-0.3419497274341694
1103	68-69	-0.48215576026225904
1103	70-71	-0.3932988146676095
1103	72-73	-0.26331367116857507
1103	74-75	-0.5417236710932016
1103	76-77	-0.4679209869975125
1103	78-79	-0.31028912420086385
1103	80-81	-0.23434334441240878
1103	82-83	-0.32426659319031614
1103	84-85	-0.393789482633494
1103	86-87	-0.2535728228730463
1103	88-89	-0.31164464954265725
1103	90-91	-0.6757300351564055
1103	92-93	-0.2022343247843068
1103	94-95	-0.41042130462125925
1103	96-97	-0.12167947061380602
1103	98-99	-0.14052676217790605
1103	100	-0.1615649594738997
1104	1	1.7092516864381118
1104	2	-0.6245802177542643
1104	3	-0.037286115639133754
1104	4	0.02554279865136344
1104	5	-1.0948018623587998
1104	6	-0.3188835036929447
1104	7	-0.19351953041939396
1104	8	0.03186147783588211
1104	9	0.07220702948507096
1104	10-11	0.05753333723863108
1104	12-13	0.12030455151124642
1104	14-15	-0.03850641140513744
1104	16-17	0.0676861765500476
1104	18-19	-0.043918105311753663
1104	20-21	0.018448476532476832
1104	22-23	0.07748058655939616
1104	24-25	0.0018405610394651717
1104	26-27	-0.09283444602825597
1104	28-29	0.040852797827618303
1104	30-31	0.06100914778696165
1104	32-33	0.11602757469911751
1104	34-35	-0.05269650215287669
1104	36-37	0.04472658211202685
1104	38-39	-0.02272261363521011
1104	40-41	0.07706341179765275
1104	42-43	-0.042710643136288695
1104	44-45	-0.14564808848627564
1104	46-47	-0.2638496901201606
1104	48-49	-0.30397603165123144
1104	50-51	0.012561569144956763
1104	52-53	-0.05216581038145307
1104	54-55	-0.05679574732972981
1104	56-57	-0.13122698669354094
1104	58-59	-0.04594860725702432
1104	60-61	-0.07247538671467879
1104	62-63	-0.2096295599724911
1104	64-65	-0.08111582364633563
1104	66-67	-0.5386676381222344
1104	68-69	-0.43980747980735657
1104	70-71	-0.26046416323591615
1104	72-73	0.021926879749386785
1104	74-75	-0.13430023219816434
1104	76-77	-0.38794547874496743
1104	78-79	-0.2836145863484347
1104	80-81	-0.32516372037537167
1104	82-83	-0.365829966806551
1104	84-85	-0.19454697042421287
1104	86-87	-0.17607829936438435
1104	88-89	-0.3256526605883394
1104	90-91	-0.3657450131772535
1104	92-93	-0.4451282540509638
1104	94-95	-0.2074625844370388
1104	96-97	-0.33976169848462945
1104	98-99	-0.3488057534301561
1104	100	-0.29920077401965983
1105	1	1.787901005248468
1105	2	0.1948220895103745
1105	3	-0.10798012696858805
1105	4	-0.16472602272492765
1105	5	-1.1422715992154906
1105	6	0.6242679892988114
1105	7	0.16205528188213947
1105	8	0.20510812073933948
1105	9	0.06204945088976643
1105	10-11	0.03207625935421987
1105	12-13	-0.11888086182936064
1105	14-15	-0.1404545974390743
1105	16-17	-0.04558695523504497
1105	18-19	-0.05583096218968109
1105	20-21	-0.033049456759805196
1105	22-23	-0.012293010350028055
1105	24-25	-0.025683130614019944
1105	26-27	-0.07598306052897641
1105	28-29	-0.09659345828330146
1105	30-31	-0.08165803720849851
1105	32-33	-0.09736659650683777
1105	34-35	-0.022611103787191666
1105	36-37	-0.19354380176631736
1105	38-39	-0.12511262549676871
1105	40-41	-0.05411273174961195
1105	42-43	-0.09597907388707227
1105	44-45	0.0074771129094486355
1105	46-47	-0.3011278400996886
1105	48-49	-0.2875458039629706
1105	50-51	-0.07816059578093615
1105	52-53	-0.09821546660657532
1105	54-55	-0.1411283009777904
1105	56-57	0.12713693687012295
1105	58-59	0.056520506740803
1105	60-61	0.05162337032958675
1105	62-63	-0.13822691751020244
1105	64-65	-0.23940211062400607
1105	66-67	-0.20946493316736792
1105	68-69	-0.17543752456488448
1105	70-71	-0.1534705385674755
1105	72-73	-0.19200935716470724
1105	74-75	-0.2020301681103973
1105	76-77	-0.30987091868718863
1105	78-79	-0.2089282657758531
1105	80-81	-0.386007636829234
1105	82-83	-0.1008088750956162
1105	84-85	-0.13856323201967058
1105	86-87	-0.2938572466596341
1105	88-89	-0.18229337887331098
1105	90-91	-0.13275821985197211
1105	92-93	-0.07243196285138964
1105	94-95	-0.024127225011774556
1105	96-97	-0.10718319243274976
1105	98-99	-0.4876421215485749
1105	100	-0.3492729899485738
1106	1	1.3100363637642296
1106	2	-0.588212253949747
1106	3	-0.06548004346584158
1106	4	-0.3194340952450929
1106	5	-1.0679213162631491
1106	6	-0.8798728087172378
1106	7	-0.38100867972086405
1106	8	-0.1874512403314128
1106	9	-0.20735837014639458
1106	10-11	-0.23203701840819235
1106	12-13	-0.1776015884871569
1106	14-15	-0.02738362160533825
1106	16-17	-0.11636210091450039
1106	18-19	-0.04823249368635629
1106	20-21	-0.20276982201020388
1106	22-23	-0.1366564660952605
1106	24-25	-0.2946317803652221
1106	26-27	-0.253186210140008
1106	28-29	-0.27852680395127294
1106	30-31	-0.15912916496095164
1106	32-33	-0.17972057084353565
1106	34-35	-0.11269855837200993
1106	36-37	-0.43464400393678915
1106	38-39	-0.26190138381279837
1106	40-41	-0.333140632175315
1106	42-43	-0.1991671712366312
1106	44-45	-0.2828110860319981
1106	46-47	-0.5320332012762847
1106	48-49	-0.35956955026773585
1106	50-51	-0.4077808402994876
1106	52-53	-0.32429624547516056
1106	54-55	-0.3218205518853452
1106	56-57	-0.2932913252632048
1106	58-59	-0.35818200018053403
1106	60-61	-0.3463328952474214
1106	62-63	-0.32305461850506845
1106	64-65	-0.6497898711103502
1106	66-67	-0.4658758800209739
1106	68-69	-0.5893967632149852
1106	70-71	-0.6947233349712647
1106	72-73	-0.46304276089054497
1106	74-75	-0.591643664856413
1106	76-77	-0.6213425448290621
1106	78-79	-0.3120295765625656
1106	80-81	-0.4731651770522234
1106	82-83	-0.36098438848738823
1106	84-85	-0.5343325700361632
1106	86-87	-0.37195652148049163
1106	88-89	-0.6818750207287181
1106	90-91	-0.6770363489250784
1106	92-93	-0.618946370546702
1106	94-95	-0.4420915931699412
1106	96-97	-0.47075330828202055
1106	98-99	-0.719201055910915
1106	100	-0.6887904679179186
1107	1	1.4539530966224525
1107	2	-0.639927220491483
1107	3	0.035830112112519075
1107	4	-0.11893070987486531
1107	5	-1.2392858408075114
1107	6	-0.3074690038401897
1107	7	-0.3836847324708117
1107	8	-0.25892139253333823
1107	9	-0.2025391557986751
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2221	5	-0.7128768739056319
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2221	7	0.29958749420371333
2221	8	0.1842710092050126
2221	9	0.1078409713664712
2221	10-11	0.062351385883758326
2221	12-13	0.22596328336012306
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2221	16-17	0.10312891578153227
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2221	34-35	0.11935352398307231
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2221	68-69	0.34022788438575446
2221	70-71	0.39355058536578014
2221	72-73	0.48736501868567217
2221	74-75	0.5248695205382816
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2221	78-79	0.307638307274118
2221	80-81	0.32209025371731315
2221	82-83	0.26984490756112933
2221	84-85	0.3184932531187883
2221	86-87	0.2913457104889048
2221	88-89	0.42199975991367467
2221	90-91	0.3322639532498002
2221	92-93	0.18489609340315383
2221	94-95	0.4641816200668245
2221	96-97	0.2545814134721738
2221	98-99	0.3092492343868969
2221	100	0.4472281892176255
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2222	2	2.0367737183396724
2222	3	0.7674166657633137
2222	4	0.7594733560518101
2222	5	3.2049100368405234
2222	6	0.3996424233326792
2222	7	0.5124431478204201
2222	8	0.44063197613530036
2222	9	0.44909760959722433
2222	10-11	0.38362210562352317
2222	12-13	0.1923384955379035
2222	14-15	0.35954039688225947
2222	16-17	0.25636438808517426
2222	18-19	0.3486242586775461
2222	20-21	0.21974649286749326
2222	22-23	0.3255809370206393
2222	24-25	0.3865703814257486
2222	26-27	0.4423117680116988
2222	28-29	0.3809455980609826
2222	30-31	0.3167493041569074
2222	32-33	0.25707985515294496
2222	34-35	0.2323210655589989
2222	36-37	0.19128674859507555
2222	38-39	0.34511035338631046
2222	40-41	0.3030922196452437
2222	42-43	0.4855025291314661
2222	44-45	0.43029677832988966
2222	46-47	0.3745584106358635
2222	48-49	0.29368773986842456
2222	50-51	0.25533542210899185
2222	52-53	0.4668978452727899
2222	54-55	0.18805384157874983
2222	56-57	0.419996762665086
2222	58-59	0.5202729970659377
2222	60-61	0.5811267476795265
2222	62-63	0.27697373419207594
2222	64-65	0.381004576666065
2222	66-67	0.6138018876741995
2222	68-69	0.5996755945031538
2222	70-71	0.4372720334688722
2222	72-73	0.5911157213966334
2222	74-75	0.6042526631162843
2222	76-77	0.5605517126164017
2222	78-79	0.702278874027563
2222	80-81	0.4547517135004142
2222	82-83	0.3084618086467188
2222	84-85	0.287042093577476
2222	86-87	0.4218864840986356
2222	88-89	0.5858138294152013
2222	90-91	0.4243903156903812
2222	92-93	0.4955227205542698
2222	94-95	0.6027788725351826
2222	96-97	0.6414484960345774
2222	98-99	0.4018971038764221
2222	100	0.7151938478010678
2223	1	1.7438294964210286
2223	2	-0.8905455007369518
2223	3	0.02134229574710389
2223	4	-0.21463686177494345
2223	5	-1.0759341987463955
2223	6	0.168398938759573
2223	7	0.49236881055190906
2223	8	0.5384111365935453
2223	9	0.4428728185002271
2223	10-11	0.3101220865206997
2223	12-13	0.2862605232752031
2223	14-15	0.2020818363617849
2223	16-17	0.26151532767113395
2223	18-19	0.13954304985094979
2223	20-21	0.15047556572565668
2223	22-23	0.31377184516880874
2223	24-25	0.3505891240234007
2223	26-27	0.3023388394398765
2223	28-29	0.2129763918105354
2223	30-31	0.24738913954515596
2223	32-33	0.23311863934017651
2223	34-35	0.2647887681444345
2223	36-37	0.23053185963705403
2223	38-39	0.2847926461616268
2223	40-41	0.13079485965528193
2223	42-43	0.26485974645063237
2223	44-45	0.33595285111709927
2223	46-47	0.2915754776431072
2223	48-49	0.18330917650982315
2223	50-51	0.19641167511768742
2223	52-53	0.3564393229579679
2223	54-55	0.4707832528297615
2223	56-57	0.7059338106786655
2223	58-59	0.5576564016255148
2223	60-61	0.4431931818375503
2223	62-63	0.5319096305783759
2223	64-65	0.4688003292899694
2223	66-67	0.5296416750324937
2223	68-69	0.5731365892689837
2223	70-71	0.5398350864273525
2223	72-73	0.5752631078576513
2223	74-75	0.6359098602410356
2223	76-77	0.544240085547159
2223	78-79	0.5694217467645615
2223	80-81	0.5477802749487353
2223	82-83	0.4832207037394838
2223	84-85	0.427410450571017
2223	86-87	0.43274698437426196
2223	88-89	0.48972799770561437
2223	90-91	0.551372233504587
2223	92-93	0.5398854776919038
2223	94-95	0.7378546561602519
2223	96-97	0.6603138975486118
2223	98-99	0.7196526314144975
2223	100	0.6535976159692183
2224	1	1.247399258792072
2224	2	-0.9826868298365365
2224	3	0.05184175802170543
2224	4	-0.23386065672829304
2224	5	-0.9872404013484335
2224	6	0.41953756445702695
2224	7	0.18300253851568726
2224	8	0.10510923400377692
2224	9	0.23921234574960692
2224	10-11	0.16769103043876754
2224	12-13	0.09027980796162183
2224	14-15	0.12382206081848324
2224	16-17	0.08684232829168792
2224	18-19	0.18846898701990966
2224	20-21	0.07450092164972233
2224	22-23	0.23481181773183124
2224	24-25	0.17437888369014587
2224	26-27	0.12606905083178077
2224	28-29	-0.014155861773772926
2224	30-31	0.043934981418964014
2224	32-33	0.2832319601549429
2224	34-35	0.1557524741425098
2224	36-37	-0.03887286357092279
2224	38-39	0.09429628218823893
2224	40-41	0.2011635485455514
2224	42-43	0.2551052912994365
2224	44-45	0.2404400151058823
2224	46-47	0.29044244858803125
2224	48-49	0.25760017146747316
2224	50-51	0.25492730228638294
2224	52-53	0.16732958309886214
2224	54-55	0.17039256241901057
2224	56-57	0.3211862282043043
2224	58-59	0.2718928270813663
2224	60-61	0.2392006714882413
2224	62-63	0.16546441185710847
2224	64-65	0.11772572918126656
2224	66-67	0.23208028882029197
2224	68-69	0.36318755005576975
2224	70-71	0.3562499937335275
2224	72-73	0.4007938262605677
2224	74-75	0.22326914945850973
2224	76-77	0.46513660497287646
2224	78-79	0.2708353243085071
2224	80-81	0.31931646821109894
2224	82-83	0.19740993830684772
2224	84-85	0.16098511112346614
2224	86-87	0.3683794183943192
2224	88-89	0.30502133788027663
2224	90-91	0.3861217412239242
2224	92-93	0.3259850298709708
2224	94-95	0.5108411409405136
2224	96-97	0.4148379993770561
2224	98-99	0.36482469906492554
2224	100	0.6191486255587293
2225	1	1.2836347593288941
2225	2	1.7923955446091266
2225	3	0.8404650104457527
2225	4	1.0201137877750313
2225	5	3.2248649943864613
2225	6	-0.020040716129315683
2225	7	0.2636879360073223
2225	8	0.3566696118046764
2225	9	0.2990810910809998
2225	10-11	0.13832306148515983
2225	12-13	0.23205114709897856
2225	14-15	0.15944714838330754
2225	16-17	0.12086720286983876
2225	18-19	0.11759529522918655
2225	20-21	0.30054581944298064
2225	22-23	0.26009663477309175
2225	24-25	0.14613637216063324
2225	26-27	0.32459318895805467
2225	28-29	0.31906900612585076
2225	30-31	0.2877851183648943
2225	32-33	0.19005228840413224
2225	34-35	0.2155680453990385
2225	36-37	0.2977386303047993
2225	38-39	0.3196510058971995
2225	40-41	0.17925201859129913
2225	42-43	0.42178106228723067
2225	44-45	0.33903990360365555
2225	46-47	0.46269151595468117
2225	48-49	0.3294617577172687
2225	50-51	0.3285395496732306
2225	52-53	0.2274256339891707
2225	54-55	0.2177178520090095
2225	56-57	0.45094058938585135
2225	58-59	0.6549673012750716
2225	60-61	0.5610840355161386
2225	62-63	0.41498003777586234
2225	64-65	0.41241409280627295
2225	66-67	0.4925305371232582
2225	68-69	0.6164876093639151
2225	70-71	0.5677150320515238
2225	72-73	0.5697410195042352
2225	74-75	0.48061470327102995
2225	76-77	0.6352922834196235
2225	78-79	0.6825322415188282
2225	80-81	0.4975387264069795
2225	82-83	0.4154597600557324
2225	84-85	0.24313842159980936
2225	86-87	0.2585747723231435
2225	88-89	0.25625726308377494
2225	90-91	0.5157675764779341
2225	92-93	0.4897805082234896
2225	94-95	0.6404799891573845
2225	96-97	0.5716124128107509
2225	98-99	0.5675363409569485
2225	100	0.6445355259348275
2226	1	1.467924281972694
2226	2	1.4111911254318965
2226	3	0.6370855371994679
2226	4	1.2064390037733954
2226	5	3.131734338807995
2226	6	-0.2448164564437505
2226	7	0.21256461855696784
2226	8	0.2481475214754596
2226	9	0.24604025792486794
2226	10-11	0.06105108692558758
2226	12-13	0.2226964044706321
2226	14-15	0.2040654160545614
2226	16-17	0.16930670221152155
2226	18-19	0.1599878221044122
2226	20-21	0.12132006086472558
2226	22-23	0.12110221542544508
2226	24-25	0.17025720825082402
2226	26-27	0.1524771681439745
2226	28-29	0.15661011824050775
2226	30-31	0.037005849762287824
2226	32-33	0.046016888456144045
2226	34-35	0.03902471569376331
2226	36-37	0.06028528083904661
2226	38-39	0.10198324364840516
2226	40-41	0.13243570222195444
2226	42-43	0.27616221150744025
2226	44-45	0.28662950560060807
2226	46-47	0.37374199670431807
2226	48-49	0.4375976405265334
2226	50-51	0.2904792153527396
2226	52-53	0.3427752620074216
2226	54-55	0.31084035740479976
2226	56-57	0.46614605178646684
2226	58-59	0.45997705185725124
2226	60-61	0.4558201659427752
2226	62-63	0.42867382652163855
2226	64-65	0.4665600166405923
2226	66-67	0.2961229894613666
2226	68-69	0.32069506707743756
2226	70-71	0.4010320341476046
2226	72-73	0.3476838281905543
2226	74-75	0.44940200424913
2226	76-77	0.5505288091301139
2226	78-79	0.2971001003102458
2226	80-81	0.15620703842265016
2226	82-83	0.14521581767900926
2226	84-85	0.037564974746786106
2226	86-87	0.2826096610324029
2226	88-89	0.09686488142997973
2226	90-91	0.3241306641524133
2226	92-93	0.09404644349770308
2226	94-95	0.5583445783177865
2226	96-97	0.48190094409596895
2226	98-99	0.39382140248712005
2226	100	0.6193131185635039
2227	1	1.3975391974417395
2227	2	1.5261867376182057
2227	3	0.6706952757387654
2227	4	-0.1575328940665841
2227	5	3.2756570535762215
2227	6	0.13010286394986892
2227	7	0.30447210183653084
2227	8	0.39583481805042453
2227	9	0.27193087774390534
2227	10-11	0.4120345710941038
2227	12-13	0.21539395544922968
2227	14-15	0.27042244470140275
2227	16-17	0.2979041636425208
2227	18-19	0.2320830623854917
2227	20-21	0.27462278866111234
2227	22-23	0.3361570533002478
2227	24-25	0.41880908045619236
2227	26-27	0.35687156868539915
2227	28-29	0.32745047925970283
2227	30-31	0.3445583752415544
2227	32-33	0.43773240138811076
2227	34-35	0.3788222138469379
2227	36-37	0.32471295897747154
2227	38-39	0.3766871571900907
2227	40-41	0.3204210043989235
2227	42-43	0.4462136430654766
2227	44-45	0.349122683170755
2227	46-47	0.2603917913270237
2227	48-49	0.3662602614106589
2227	50-51	0.45986132572412686
2227	52-53	0.2420646283839858
2227	54-55	0.40128996322929567
2227	56-57	0.5018633963484191
2227	58-59	0.5323578778858007
2227	60-61	0.4343066305218386
2227	62-63	0.4616993318199505
2227	64-65	0.4775535284981416
2227	66-67	0.4301852988415078
2227	68-69	0.38430586361816665
2227	70-71	0.5952493752575307
2227	72-73	0.4400225356859977
2227	74-75	0.4983343837764451
2227	76-77	0.6407563652718409
2227	78-79	0.3682276518057108
2227	80-81	0.31005294825816065
2227	82-83	0.3843789068267327
2227	84-85	0.48032914996002773
2227	86-87	0.5620692630111463
2227	88-89	0.34075464327889904
2227	90-91	0.41501625240027096
2227	92-93	0.5197181111337343
2227	94-95	0.6064056460856762
2227	96-97	0.4761223343471599
2227	98-99	0.6058212845582176
2227	100	0.6887236102430805
2228	1	1.600126289358787
2228	2	1.9312103873648407
2228	3	-0.2206666108254769
2228	4	0.09992434022766261
2228	5	3.1092243224914355
2228	6	0.11847340444765209
2228	7	0.21513021442705593
2228	8	0.3201808736646399
2228	9	0.17039984322503443
2228	10-11	0.12709925587197546
2228	12-13	0.18874381200239299
2228	14-15	0.1380448251745321
2228	16-17	0.10906812686657474
2228	18-19	0.17275795251430992
2228	20-21	0.0037172503904869814
2228	22-23	0.1924502944506301
2228	24-25	0.15700617950538032
2228	26-27	0.1654244667377398
2228	28-29	0.2419111202992994
2228	30-31	0.13383281215992326
2228	32-33	0.08929068164182041
2228	34-35	0.1943597065586502
2228	36-37	0.25195572053185344
2228	38-39	0.361345022194115
2228	40-41	0.14839532421859758
2228	42-43	0.3956225022489761
2228	44-45	0.2894298400287667
2228	46-47	0.248809549038576
2228	48-49	0.09680340335813753
2228	50-51	0.043170723202557326
2228	52-53	0.10285407027839
2228	54-55	0.16527673044151214
2228	56-57	0.46601122568427655
2228	58-59	0.2603279154314393
2228	60-61	0.19064925807283117
2228	62-63	0.24710429469507034
2228	64-65	0.247481343195723
2228	66-67	0.3786269998879135
2228	68-69	0.3355314167888608
2228	70-71	0.34091277804598263
2228	72-73	0.39759557837820836
2228	74-75	0.42386638620144623
2228	76-77	0.47203373956838846
2228	78-79	0.4971771146140753
2228	80-81	0.40630444378159325
2228	82-83	0.369230222158059
2228	84-85	0.48244700562159437
2228	86-87	0.5381253671590329
2228	88-89	0.5204457809912242
2228	90-91	0.4296867809675362
2228	92-93	0.5256988023787912
2228	94-95	0.7197278941262084
2228	96-97	0.5572041078294561
2228	98-99	0.7016007014433896
2228	100	0.613237029842729
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	24.0
13	19.0
14	113.0
15	438.0
16	1715.0
17	4464.0
18	7177.0
19	9306.0
20	10806.0
21	12619.0
22	14381.0
23	16121.0
24	18520.0
25	20912.0
26	23349.0
27	26426.0
28	29644.0
29	34094.0
30	38791.0
31	44841.0
32	52704.0
33	62775.0
34	77169.0
35	98998.0
36	139004.0
37	228783.0
38	561017.0
39	1072196.0
40	1402.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.37968771834342	19.53588219286773	19.240611584385313	35.84381850440354
2	18.548376581790627	52.513454663708195	12.159702659026571	16.778466095474613
3	41.274265101470235	15.479201122474844	23.683789725397833	19.562744050657084
4	17.146079333264055	16.648190109014884	22.401525571849778	43.80420498587128
5	17.36883337698428	34.390708828752416	26.761929267059053	21.478528527204254
6	17.401236056781766	27.994534875828712	30.069418427499865	24.534810639889653
7	20.466567911990488	26.229144849181825	36.01839705285165	17.285890185976044
8	21.660328651369788	28.173420676130807	33.29446289969856	16.871787772800843
9	21.13885641164671	28.488934580715075	26.92819911274177	23.444009894896446
10-11	19.594993344988072	30.371568623315586	28.669871911631567	21.363566120064775
12-13	24.195253563431983	26.751019725754453	27.241431408511897	21.812295302301663
14-15	21.52847466973233	27.63705087297421	28.631755623207066	22.20271883408639
16-17	20.785839760503933	29.982391348446146	28.35882535875902	20.8729435322909
18-19	21.375530186451538	29.683673152443298	29.706911050617585	19.233885610487576
20-21	22.620828442573824	29.089036045201162	30.010422542448467	18.279712969776543
22-23	21.638490395577286	30.701903398600127	28.659633431742893	18.999972774079694
24-25	22.2150663641394	28.374374810425152	26.581432919358743	22.82912590607671
26-27	20.000851289339057	26.7310029223766	30.166453908242513	23.101691880041827
28-29	19.570780132379735	28.25207905051037	30.70475853027129	21.472382286838602
30-31	20.045771022168363	30.341106289632584	28.290441775693232	21.322680912505824
32-33	20.536588377446353	30.358722590496466	28.79808298843972	20.30660604361746
34-35	21.146314779955127	29.266656415404658	30.299496627245325	19.287532177394894
36-37	21.67388409197146	28.2972602671438	28.825289735559622	21.20356590532512
38-39	21.393476003417454	29.28708161859783	29.071630822750926	20.247811555233785
40-41	20.766282922519448	29.808336278058214	30.267387410050077	19.157993389372262
42-43	20.533107242852275	29.996523897200156	29.471772338849654	19.998596521097912
44-45	20.538582388510815	30.195290375943944	28.983698576084365	20.282428659460873
46-47	19.593333287316863	28.299553410165494	28.932203168847465	23.174910133670174
48-49	20.785747879876915	28.164280467680047	30.357175383085067	20.69279626935797
50-51	21.67144909769082	31.04055933544213	28.269593363624406	19.018398203242644
52-53	20.204335152445122	30.598581280705062	28.28149059667544	20.915592970174377
54-55	22.15210749453269	28.705211570221305	28.14835497373926	20.994325961506746
56-57	21.22591056723181	28.765134574076622	29.522711784325583	20.48624307436598
58-59	21.076395620444394	28.15611596614065	28.89594307966052	21.871545333754437
60-61	19.527276499168742	30.479367022860426	29.865612957417998	20.127743520552833
62-63	20.419554244468515	31.317306905009406	27.54114996114102	20.721988889381056
64-65	19.438562531108524	30.451249057636957	29.11990002323793	20.99028838801659
66-67	20.195382407223843	30.40836196209155	29.622779122243998	19.77347650844061
68-69	20.68903435796103	30.24218798232539	29.276300526610655	19.792477133102924
70-71	19.585636831531758	31.077506059684584	29.37099687898922	19.965860229794437
72-73	19.985474396322736	30.89800671751651	28.690789854625088	20.42572903153567
74-75	19.83772584571953	29.372223962721193	30.5303992738732	20.259650917686074
76-77	19.23988681686427	30.82328882214917	28.77060781675345	21.16621654423311
78-79	19.24793955385536	29.714829575325492	28.49737078137241	22.539860089446734
80-81	20.84968809005585	29.551607406078062	28.383977476834914	21.21472702703117
82-83	19.882801999686325	28.203906037597076	30.30524858223896	21.608043380477636
84-85	20.512756110589386	29.401443894088867	28.33602844380091	21.749771551520837
86-87	20.996246274171153	29.74671457917355	28.92976441142737	20.327274735227924
88-89	20.317707316755175	29.143564578540836	29.430165322690428	21.10856278201356
90-91	19.988331200636242	29.36978896844056	30.080462181087647	20.56141764983555
92-93	19.888592301046586	28.64406480689345	31.56632636822712	19.901016523832844
94-95	19.87622559781027	29.142337494808864	31.185949584497948	19.795487322882927
96-97	19.47594704980311	29.20037472069465	31.26020732346579	20.063470906036446
98-99	21.154309997396282	29.673223767538193	29.895057498459437	19.277408736606095
100	20.051430146916434	31.06830293169477	29.61348012833762	19.26678679305118
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	7.0
2	20.5
3	30.0
4	29.0
5	34.0
6	41.5
7	47.5
8	60.0
9	75.0
10	77.0
11	89.0
12	101.0
13	100.0
14	139.5
15	194.5
16	233.5
17	314.0
18	467.5
19	743.0
20	1206.5
21	1862.0
22	2858.0
23	4453.5
24	6885.0
25	10296.5
26	14572.0
27	19812.0
28	25740.0
29	32602.0
30	40557.5
31	49650.0
32	59906.5
33	71355.5
34	83783.5
35	97328.0
36	111041.5
37	119963.0
38	125009.0
39	128696.5
40	129834.5
41	128544.5
42	125108.5
43	121968.5
44	120456.0
45	116040.5
46	106711.0
47	96545.0
48	88372.0
49	83834.5
50	80917.0
51	74707.5
52	64527.5
53	53496.5
54	44447.5
55	36908.0
56	29402.0
57	21684.0
58	14936.5
59	10443.0
60	8026.5
61	6819.0
62	5619.0
63	4220.0
64	3145.5
65	2711.5
66	3056.0
67	3108.5
68	2163.0
69	1862.0
70	2472.5
71	2306.5
72	1279.5
73	499.0
74	226.5
75	130.5
76	97.0
77	81.5
78	60.5
79	51.5
80	55.5
81	45.0
82	27.0
83	22.0
84	21.0
85	16.0
86	15.0
87	17.0
88	18.0
89	20.0
90	16.0
91	12.0
92	11.5
93	13.0
94	14.0
95	14.0
96	13.0
97	15.0
98	38.0
99	82.5
100	87.5
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.36471267642684
2	0.006250457759751577
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	3.067709329939424E-4
30-31	0.0101234407888001
32-33	0.0
34-35	0.0
36-37	0.0
38-39	8.052736991090989E-4
40-41	0.001476335115033348
42-43	1.342122831848498E-4
44-45	0.0
46-47	0.0018022792313394118
48-49	1.91731833121214E-5
50-51	0.0
52-53	0.0013229496485363766
54-55	0.018981451479000187
56-57	0.010967060854533441
58-59	0.014399060667403171
60-61	2.109050164333354E-4
62-63	0.01163812227045769
64-65	1.1503909987272841E-4
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.9173183312121402E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2607809.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.6028821426196
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.07457445497853	63.35336759085921
2	8.513701114938668	12.5326587952064
3	2.4325862334558366	5.371360735284267
4	1.0499197780037515	3.0910848671846187
5	0.567193510351816	2.0873538547241703
6	0.3331901318017186	1.4714252401251473
7	0.2338404021017515	1.2047929289254597
8	0.1490316296320588	0.8775325977064768
9	0.11199859327176309	0.7419077334648693
>10	0.49813839473042737	6.269246693427226
>50	0.025057330825155214	1.2679800368044158
>100	0.010024543205485898	1.3514279900379977
>500	6.907482242404968E-4	0.33386933107968847
>1k	5.3134478785640064E-5	0.04599160517008329
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	5.7519549936364204E-5	0.0	0.0	0.0	0.0
12-13	7.66927332484856E-5	0.0	0.0	0.0	0.0
14-15	7.66927332484856E-5	0.0	0.0	0.0	0.0
16-17	7.66927332484856E-5	0.0	0.0	0.0	0.0
18-19	7.66927332484856E-5	0.0	0.0	0.0	0.0
20-21	7.66927332484856E-5	0.0	0.0	0.0	0.0
22-23	7.66927332484856E-5	0.0	0.0	0.0	0.0
24-25	7.66927332484856E-5	0.0	0.0	0.0	0.0
26-27	7.66927332484856E-5	0.0	0.0	0.0	0.0
28-29	7.66927332484856E-5	0.0	0.0	0.0	0.0
30-31	7.66927332484856E-5	0.0	0.0	0.0	0.0
32-33	7.66927332484856E-5	0.0	0.0	0.0	0.0
34-35	7.66927332484856E-5	0.0	0.0	0.0	0.0
36-37	7.66927332484856E-5	0.0	0.0	0.0	0.0
38-39	7.66927332484856E-5	0.0	0.0	0.0	0.0
40-41	7.66927332484856E-5	0.0	0.0	0.0	0.0
42-43	7.66927332484856E-5	0.0	0.0	0.0	0.0
44-45	7.66927332484856E-5	0.0	0.0	0.0	0.0
46-47	7.66927332484856E-5	0.0	0.0	0.0	0.0
48-49	7.66927332484856E-5	0.0	0.0	0.0	0.0
50-51	9.5865916560607E-5	0.0	0.0	0.0	0.0
52-53	1.1503909987272841E-4	0.0	0.0	0.0	0.0
54-55	1.1503909987272841E-4	0.0	0.0	0.0	0.0
56-57	1.533854664969712E-4	0.0	1.1503909987272841E-4	0.0	0.0
58-59	2.3007819974545682E-4	0.0	2.3007819974545682E-4	0.0	0.0
60-61	2.6842456636969964E-4	0.0	2.3007819974545682E-4	0.0	0.0
62-63	3.067709329939424E-4	0.0	2.3007819974545682E-4	0.0	0.0
64-65	3.067709329939424E-4	0.0	2.3007819974545682E-4	0.0	0.0
66-67	3.2594411630606384E-4	0.0	2.3007819974545682E-4	0.0	0.0
68-69	3.8346366624242805E-4	0.0	2.3007819974545682E-4	1.533854664969712E-4	0.0
70-71	4.0263684955454943E-4	0.0	2.3007819974545682E-4	3.067709329939424E-4	0.0
72-73	4.4098321617879225E-4	0.0	2.3007819974545682E-4	3.067709329939424E-4	0.0
74-75	4.985027661151564E-4	0.0	2.3007819974545682E-4	3.067709329939424E-4	0.0
76-77	4.985027661151564E-4	0.0	2.3007819974545682E-4	3.067709329939424E-4	0.0
78-79	6.135418659878849E-4	0.0	2.3007819974545682E-4	3.067709329939424E-4	0.0
80-81	8.244468824212202E-4	0.0	2.3007819974545682E-4	3.067709329939424E-4	0.0
82-83	9.394859822939486E-4	0.0	2.3007819974545682E-4	3.067709329939424E-4	0.0
84-85	9.970055322303128E-4	0.0	2.3007819974545682E-4	3.067709329939424E-4	0.0
86-87	0.0010736982654787986	0.0	2.3007819974545682E-4	3.067709329939424E-4	0.0
88	0.0010736982654787986	0.0	2.3007819974545682E-4	3.067709329939424E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	10425	0.0	81.537674	8
CACCGGC	10795	0.0	77.221146	9
GCAGCCT	55	5.638867E-11	77.13368	1
CTTCACC	11690	0.0	75.92642	6
AGGCAGC	65	0.0	72.21502	2
CCTTCAC	12990	0.0	72.121925	5
CGGGCCC	870	0.0	71.21485	7
GTGTGGC	4100	0.0	70.86351	7
GGCCCGA	890	0.0	67.50499	9
TTCACCG	13075	0.0	66.878586	7
CGTAGTG	2430	0.0	66.8322	4
GGGCCCG	945	0.0	64.5695	8
CGGGGCA	810	0.0	64.32116	94
CGCTGCA	50	1.9822801E-7	63.63529	1
AACGGGC	980	0.0	63.221348	5
ACCTTCA	5245	0.0	63.00037	4
ACGGGCC	1015	0.0	61.966175	6
TGTGGCA	4765	0.0	61.761887	8
CAGCGTA	1100	0.0	61.706947	1
GGCGCGC	355	0.0	60.81986	6
>>END_MODULE