FastQCFastQC Report
Tue 7 Mar 2017
1SN_27A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_27A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2607809
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG104250.081.5376748
CACCGGC107950.077.2211469
GCAGCCT555.638867E-1177.133681
CTTCACC116900.075.926426
AGGCAGC650.072.215022
CCTTCAC129900.072.1219255
CGGGCCC8700.071.214857
GTGTGGC41000.070.863517
GGCCCGA8900.067.504999
TTCACCG130750.066.8785867
CGTAGTG24300.066.83224
GGGCCCG9450.064.56958
CGGGGCA8100.064.3211694
CGCTGCA501.9822801E-763.635291
AACGGGC9800.063.2213485
ACCTTCA52450.063.000374
ACGGGCC10150.061.9661756
TGTGGCA47650.061.7618878
CAGCGTA11000.061.7069471
GGCGCGC3550.060.819866
Produced by FastQC (version 0.11.5)