FastQCFastQC Report
Tue 7 Mar 2017
1SN_28A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_28A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2841587
Sequences flagged as poor quality0
Sequence length93
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA147630.5195336268078365No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG94180.33143451177106314No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG72400.2547872016587914No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA62730.22075692210022074No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA54750.1926740233538512No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT54120.19045695240019048No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG53230.18732489978311417No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG52750.1856357028660393No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA49640.17469111450749175No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC48720.1714534870830983No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG46330.16304269410016306No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT45810.16121273077333192No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA44550.15677858886601045No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA41760.14696013178551282No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC41230.14509497685624265No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA37650.13249638318305934No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTTAAAAAAAAGCCATGAGTGCTC37030.13031450383183762No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA36270.12763994204646908No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT35580.125211721478174No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA34080.11993298111231505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCT402.0008883E-1186.999883
AGCTGCG356.6393113E-1086.999882
AGCTGCA800.086.999882
CAGCTGC3850.086.999881
GCAGCTT159.3885924E-486.999883
GCAGCCT202.69923E-586.999883
AGCAGCG750.086.999882
AGCAGCC402.0008883E-1186.999882
GCTGCCC1450.086.999873
AGCTGCC2300.085.108572
CAGCCCT162400.082.1248861
CAGCGTA77050.081.8058551
GCTGCCA650.080.307583
AGCCCTT143400.080.0835652
GCTGCTA550.079.09083
AGCTGCT501.8189894E-1278.29992
CAGCAGC2300.077.5433651
CAGCATG103150.077.5112841
GCCCTTC132300.077.333223
CAGCGTG53600.076.611831
Produced by FastQC (version 0.11.5)