FastQCFastQC Report
Tue 7 Mar 2017
1SN_28A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_28A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2841587
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG117050.081.725468
CACCGGC118150.078.620659
CTTCACC130250.076.5422746
CCTTCAC143850.073.580255
CGGGCCC9450.072.5179757
CGTAGTG26900.071.192214
GTGTGGC42000.069.959987
TTCACCG143050.068.80747
ATGCAGC1100.068.275862
TGCAGCA700.067.054283
GGGCCCG10300.066.533488
ACGGGCC10450.066.027616
GGCCCGA10450.064.6801159
CGGGGCA10700.064.0462594
GCGTAGT18750.063.335013
ACCTTCA60300.061.416394
AACGGGC11300.061.0609365
TGTGGCA50600.060.574148
AGCGTAG15600.060.179042
CAGCGTA11300.059.9387251
Produced by FastQC (version 0.11.5)