##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1SN_28A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2841587
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.819368543000795	12.0	12.0	12.0	2.0	32.0
2	14.63065850174568	12.0	12.0	12.0	12.0	22.0
3	13.692153011679741	12.0	12.0	12.0	12.0	22.0
4	13.766136317487376	12.0	12.0	12.0	12.0	22.0
5	22.434472004552386	22.0	22.0	22.0	22.0	27.0
6	26.80643316569227	27.0	22.0	32.0	22.0	32.0
7	34.96686041989916	37.0	37.0	37.0	27.0	41.0
8	37.584742258463315	41.0	37.0	41.0	27.0	41.0
9	38.51815798706849	41.0	37.0	41.0	32.0	41.0
10-11	38.87218867484965	41.0	41.0	41.0	37.0	41.0
12-13	39.05501028122666	41.0	41.0	41.0	37.0	41.0
14-15	39.00630052854268	41.0	41.0	41.0	37.0	41.0
16-17	38.92232544701253	41.0	41.0	41.0	37.0	41.0
18-19	38.86003419919925	41.0	41.0	41.0	37.0	41.0
20-21	38.87980149824728	41.0	41.0	41.0	37.0	41.0
22-23	38.86066324909285	41.0	41.0	41.0	37.0	41.0
24-25	38.87531351318823	41.0	41.0	41.0	37.0	41.0
26-27	38.842333527004456	41.0	41.0	41.0	37.0	41.0
28-29	38.79108522807853	41.0	41.0	41.0	34.5	41.0
30-31	38.6811897717719	41.0	41.0	41.0	32.0	41.0
32-33	38.71881487351962	41.0	41.0	41.0	32.0	41.0
34-35	38.69465513461316	41.0	41.0	41.0	32.0	41.0
36-37	38.61510574900575	41.0	41.0	41.0	32.0	41.0
38-39	38.52391691684963	41.0	41.0	41.0	32.0	41.0
40-41	38.569393968933554	41.0	41.0	41.0	32.0	41.0
42-43	38.32075280468274	41.0	41.0	41.0	32.0	41.0
44-45	38.431624651999044	41.0	41.0	41.0	32.0	41.0
46-47	38.44208905094231	41.0	41.0	41.0	32.0	41.0
48-49	38.39013797571568	41.0	41.0	41.0	32.0	41.0
50-51	38.284070134048335	41.0	41.0	41.0	32.0	41.0
52-53	38.2536489292779	41.0	41.0	41.0	32.0	41.0
54-55	38.120517513628826	41.0	39.0	41.0	32.0	41.0
56-57	37.899683873835286	41.0	37.0	41.0	29.5	41.0
58-59	37.89074942980807	41.0	37.0	41.0	29.5	41.0
60-61	37.90179220273741	41.0	37.0	41.0	29.5	41.0
62-63	37.89449821525788	41.0	37.0	41.0	29.5	41.0
64-65	37.87540853051482	41.0	37.0	41.0	29.5	41.0
66-67	37.751642128148816	41.0	37.0	41.0	27.0	41.0
68-69	37.77023402767538	41.0	37.0	41.0	27.0	41.0
70-71	37.74289384769848	41.0	37.0	41.0	27.0	41.0
72-73	37.595653238841535	41.0	37.0	41.0	27.0	41.0
74-75	37.521608699645654	41.0	37.0	41.0	27.0	41.0
76-77	36.46136841842252	39.0	34.5	41.0	24.5	41.0
78-79	36.969567709874795	41.0	37.0	41.0	27.0	41.0
80-81	37.60466352077202	41.0	37.0	41.0	27.0	41.0
82-83	37.80713682178303	41.0	37.0	41.0	27.0	41.0
84-85	37.7150358232917	41.0	37.0	41.0	27.0	41.0
86-87	37.58483850749599	41.0	37.0	41.0	27.0	41.0
88-89	37.49102948458027	41.0	37.0	41.0	27.0	41.0
90-91	37.329682145927606	41.0	37.0	41.0	27.0	41.0
92-93	37.22670782207267	41.0	37.0	41.0	27.0	41.0
94-95	37.082741263948634	41.0	37.0	41.0	27.0	41.0
96-97	37.02643804324837	41.0	37.0	41.0	27.0	41.0
98-99	36.9019204761283	41.0	37.0	41.0	27.0	41.0
100	36.77087768208399	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.4773988031797352
1101	2	-0.41411196333457667
1101	3	-0.15187250105040562
1101	4	0.09601527476871752
1101	5	-1.1053983760197426
1101	6	-0.81192928420678
1101	7	-0.29338767533262455
1101	8	-0.20080223252396223
1101	9	-0.29398214843642023
1101	10-11	-0.1676429852997785
1101	12-13	-0.20204787114333556
1101	14-15	-0.17469182419534235
1101	16-17	-0.25970525884714135
1101	18-19	-0.05789366185612721
1101	20-21	0.02020161304437096
1101	22-23	-0.09162812569846324
1101	24-25	-0.08006469327892773
1101	26-27	-0.14151701954051532
1101	28-29	-0.018704930789908758
1101	30-31	-0.07319271006890204
1101	32-33	-0.10642324171324447
1101	34-35	-0.13109154981476223
1101	36-37	-0.12158479355883856
1101	38-39	-0.13642871110535282
1101	40-41	-0.1521546468466397
1101	42-43	0.0841725180538404
1101	44-45	-0.0787427176286215
1101	46-47	-0.09111941717679883
1101	48-49	-0.1796806168294509
1101	50-51	-0.13141072592600977
1101	52-53	-0.032496833125669866
1101	54-55	-0.18873989074903363
1101	56-57	0.04694870709600707
1101	58-59	-0.24177677034013811
1101	60-61	-0.28596152064741887
1101	62-63	-0.35076645317953137
1101	64-65	-0.28128223586065815
1101	66-67	-0.22213274932565952
1101	68-69	-0.20091045946355024
1101	70-71	-0.12326208815186845
1101	72-73	-0.042868093672964847
1101	74-75	0.016135671940034513
1101	76-77	-0.4842184278998545
1101	78-79	-0.2613689381976769
1101	80-81	-0.18094647811337694
1101	82-83	-0.3225502194052581
1101	84-85	-0.1479231482102179
1101	86-87	-0.15326620409880576
1101	88-89	-0.3347184770393312
1101	90-91	-0.2545117821194296
1101	92-93	0.026176841510192617
1101	94-95	-0.0948714475737873
1101	96-97	-0.2090563068015996
1101	98-99	-0.37013842260951435
1101	100	-0.47500587622675283
1102	1	1.7195947161772764
1102	2	0.8819912631928428
1102	3	0.04090260714391647
1102	4	0.05064223293357806
1102	5	-0.7418902131898264
1102	6	-0.03628710264349877
1102	7	0.005057221755777164
1102	8	-0.3887409782825415
1102	9	-0.20067597374067958
1102	10-11	-0.24183800429727853
1102	12-13	-0.21571597616802052
1102	14-15	-0.2719806801800644
1102	16-17	-0.24499394212730152
1102	18-19	-0.12315280757813696
1102	20-21	-4.0229414217662907E-4
1102	22-23	-0.16959851702666384
1102	24-25	-0.12690762864678362
1102	26-27	-0.1534209348543243
1102	28-29	-0.11139362321326018
1102	30-31	-0.15307047080198544
1102	32-33	-0.16168774635594474
1102	34-35	-0.06852805549369378
1102	36-37	-0.29220133611527643
1102	38-39	-0.2798506359483923
1102	40-41	-0.2712658660391938
1102	42-43	-0.19253986029103487
1102	44-45	-0.16637345243569968
1102	46-47	-0.5070234112235781
1102	48-49	-0.2959339566307406
1102	50-51	-0.3405924492126786
1102	52-53	-0.3411573315306953
1102	54-55	-0.26555976497948564
1102	56-57	-0.22738993605574365
1102	58-59	-0.2355003567025733
1102	60-61	-0.305394592553462
1102	62-63	-0.3016402620260692
1102	64-65	-0.2561572155424727
1102	66-67	-0.1719906490351022
1102	68-69	-0.35421559285241955
1102	70-71	-0.38636890337831176
1102	72-73	-0.1033238940061878
1102	74-75	-0.2854134625053888
1102	76-77	-0.289634366498305
1102	78-79	-0.46437107668640465
1102	80-81	-0.3788449430746681
1102	82-83	-0.4358974241312623
1102	84-85	-0.5168721394124347
1102	86-87	-0.31389953455373387
1102	88-89	-0.44668658511174186
1102	90-91	-0.4587914263480499
1102	92-93	-0.34802344751595626
1102	94-95	-0.3581966832647012
1102	96-97	-0.4190139306917189
1102	98-99	-0.5265604922196019
1102	100	-0.5734715994524677
1103	1	1.4337662506546138
1103	2	0.8162270031276453
1103	3	0.27431012977303126
1103	4	1.1584875441149265
1103	5	2.9259168725774103
1103	6	0.11083970313499236
1103	7	-0.6600355286537791
1103	8	-0.37767927154262537
1103	9	-0.3391315677239035
1103	10-11	-0.29018686498136503
1103	12-13	-0.41067071793962384
1103	14-15	-0.3942532720617109
1103	16-17	-0.5084905746503452
1103	18-19	-0.44235479000437294
1103	20-21	-0.4183554142295449
1103	22-23	-0.20224815049292744
1103	24-25	-0.21929100803816226
1103	26-27	-0.35675126200429474
1103	28-29	-0.19097827347553675
1103	30-31	-0.1588479691058282
1103	32-33	-0.281343883106949
1103	34-35	-0.3395617964544684
1103	36-37	-0.32465090669990815
1103	38-39	-0.16101022291534406
1103	40-41	-0.27525305804329747
1103	42-43	-0.218386776608817
1103	44-45	-0.6426110622717118
1103	46-47	-0.5921683699368145
1103	48-49	-0.5245024065984722
1103	50-51	-0.2543251360786911
1103	52-53	-0.2867779567065156
1103	54-55	-0.3184170733146132
1103	56-57	-0.3269590313876165
1103	58-59	-0.38787637232368866
1103	60-61	-0.16259958667900065
1103	62-63	-0.21014773335700454
1103	64-65	-0.2980234762364944
1103	66-67	-0.4816256046042682
1103	68-69	-0.36047802438590537
1103	70-71	-0.5785278946245285
1103	72-73	-0.20729023578440575
1103	74-75	-0.3593388484827784
1103	76-77	-0.6263368867279908
1103	78-79	-0.5111430149300276
1103	80-81	-0.2958409385074816
1103	82-83	-0.37275648653301374
1103	84-85	-0.4914495086434627
1103	86-87	-0.3468769799046285
1103	88-89	-0.2674335527277023
1103	90-91	-0.5421541871827245
1103	92-93	-0.3242439303807174
1103	94-95	-0.27429032725536473
1103	96-97	-0.1252256432233665
1103	98-99	-0.3408920439066492
1103	100	-0.20473998819334582
1104	1	1.7701603341675387
1104	2	-0.4634836337084991
1104	3	0.007100668628092777
1104	4	0.3590118001579796
1104	5	-1.1191601505315667
1104	6	-0.3073752909149903
1104	7	-0.06030035565337499
1104	8	-0.018868294062734492
1104	9	-0.028031325224809223
1104	10-11	-0.1438898219980871
1104	12-13	-0.02992620892872111
1104	14-15	-0.21703730883324823
1104	16-17	0.032877316507232024
1104	18-19	0.06785691619959522
1104	20-21	-0.04276848873360706
1104	22-23	0.09753715806978391
1104	24-25	-0.02116090911652435
1104	26-27	0.03632078844205466
1104	28-29	-0.0772725356935311
1104	30-31	0.08954707949622076
1104	32-33	-0.05238311234425197
1104	34-35	-0.19817959253440165
1104	36-37	-0.028419988679324604
1104	38-39	-0.0617984062986352
1104	40-41	0.013197769438846763
1104	42-43	0.04006556393678551
1104	44-45	-0.08927238550705141
1104	46-47	-0.4267228954278508
1104	48-49	-0.2649578795943839
1104	50-51	-0.19883839992014885
1104	52-53	-0.10884741858753699
1104	54-55	-0.18039935002529006
1104	56-57	-0.11640758109583516
1104	58-59	-0.2530819649334006
1104	60-61	-0.08453671684744535
1104	62-63	-0.22745276286174487
1104	64-65	-0.12178004477212312
1104	66-67	-0.4743399995629005
1104	68-69	-0.4545879545805249
1104	70-71	-0.321300072249997
1104	72-73	-0.1375295042795628
1104	74-75	-0.13293131097312028
1104	76-77	-0.2820023279495629
1104	78-79	-0.34110657872609806
1104	80-81	-0.43244104401298955
1104	82-83	-0.2369440736875177
1104	84-85	-0.1045259755993797
1104	86-87	-0.22765894745963777
1104	88-89	-0.2220391134403954
1104	90-91	-0.3358392327995503
1104	92-93	-0.32378404087523904
1104	94-95	-0.2420511977984816
1104	96-97	-0.177444080515059
1104	98-99	-0.21812231989760278
1104	100	-0.23380569195187917
1105	1	1.5339101854364348
1105	2	0.3819947157741499
1105	3	-0.04613304421065578
1105	4	-0.2027395704563535
1105	5	-1.138857811028135
1105	6	0.41258393465630405
1105	7	-0.020846240304436492
1105	8	0.12066777658481698
1105	9	0.02277304826858284
1105	10-11	-0.002889089318031779
1105	12-13	-0.13459396884979924
1105	14-15	-0.11145722805073177
1105	16-17	-0.06751125368385402
1105	18-19	-0.09538173443425535
1105	20-21	-0.005962911739914034
1105	22-23	0.054906053249375475
1105	24-25	-0.032458500549118696
1105	26-27	-0.10436658118068465
1105	28-29	-0.08857927980947977
1105	30-31	-0.037086965352237655
1105	32-33	-0.1477003978693432
1105	34-35	-0.08906463684812849
1105	36-37	-0.11863411027690773
1105	38-39	-0.12624082095025102
1105	40-41	-0.12197138533566942
1105	42-43	0.04286380296050396
1105	44-45	0.014464360360314288
1105	46-47	-0.24114820697200656
1105	48-49	-0.04310676334707608
1105	50-51	0.076565687171815
1105	52-53	-0.04688786320938476
1105	54-55	-0.030757243214104335
1105	56-57	-0.01608191621156152
1105	58-59	-0.013634892496739326
1105	60-61	-0.077428417178659
1105	62-63	-0.15113745633294684
1105	64-65	-0.20267704497240402
1105	66-67	-0.21265625021425905
1105	68-69	-0.3207714578150629
1105	70-71	-0.4044867945918682
1105	72-73	-0.19616710098969037
1105	74-75	-0.24054693302127816
1105	76-77	-0.20913696252556946
1105	78-79	-0.23130920517390763
1105	80-81	-0.3279370240458661
1105	82-83	-0.28001039635807246
1105	84-85	-0.36289179966588136
1105	86-87	-0.4284894526396741
1105	88-89	-0.24062805820891953
1105	90-91	-0.4238358453759261
1105	92-93	-0.3515096130226567
1105	94-95	-0.2506530912869991
1105	96-97	-0.2683410853692436
1105	98-99	-0.4851026805660723
1105	100	-0.4126506760564581
1106	1	1.522193802484228
1106	2	-0.5021743304430544
1106	3	-0.20319719353694232
1106	4	-0.2717961633410937
1106	5	-1.1256457032856062
1106	6	-0.5807458541648529
1106	7	-0.21732032044519656
1106	8	-0.0550357589432906
1106	9	-0.061320144978751046
1106	10-11	-0.02241056175586209
1106	12-13	-0.12410854667936633
1106	14-15	-0.0475019085504087
1106	16-17	0.0689270886877793
1106	18-19	0.06916149930277982
1106	20-21	0.1056160855519721
1106	22-23	0.0031961842592096446
1106	24-25	-0.22417933767611942
1106	26-27	-0.2805662614378548
1106	28-29	-0.1618716363718562
1106	30-31	-0.013809450922984468
1106	32-33	-0.052106316549803466
1106	34-35	0.008994742735360717
1106	36-37	-0.27233997757012673
1106	38-39	0.018770886990949975
1106	40-41	-0.18282681712662452
1106	42-43	0.08769755879068697
1106	44-45	-0.0681514497193163
1106	46-47	-0.33542153797644403
1106	48-49	-0.30795043132779654
1106	50-51	-0.15413424449604918
1106	52-53	-0.1397744447545506
1106	54-55	-0.28181177140002944
1106	56-57	-0.18993601758256773
1106	58-59	-0.12496201863613976
1106	60-61	-0.17833305056415583
1106	62-63	-0.30821073809894983
1106	64-65	-0.41912707072741995
1106	66-67	-0.46821990132374935
1106	68-69	-0.36961872904208093
1106	70-71	-0.6543186502606417
1106	72-73	-0.4877364317092514
1106	74-75	-0.7562652431484977
1106	76-77	-0.4321740098405158
1106	78-79	-0.27166430512731665
1106	80-81	-0.2194160894243069
1106	82-83	-0.23560594193769901
1106	84-85	-0.5292634399257352
1106	86-87	-0.47329972505343676
1106	88-89	-0.37712111847564955
1106	90-91	-0.6084571699142813
1106	92-93	-0.48696917273776563
1106	94-95	-0.43254228148670393
1106	96-97	-0.42678384937656233
1106	98-99	-0.5513254543842407
1106	100	-0.5542452399371882
1107	1	1.6535063348758978
1107	2	-0.530939387471216
1107	3	-0.1878405620446486
1107	4	-0.3323066246737536
1107	5	-1.2562507953501019
1107	6	-0.29477180765637456
1107	7	-0.5267471902523368
1107	8	-0.2612086542220453
1107	9	-0.16271501024823465
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2224	36-37	0.32836700989305
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2224	64-65	0.4343528847973346
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2224	70-71	0.7436108383474718
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2224	76-77	0.7798597776334333
2224	78-79	0.618849988913837
2224	80-81	0.492479019033091
2224	82-83	0.5582848953284483
2224	84-85	0.40664818021254234
2224	86-87	0.5670139283090236
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2224	92-93	0.5129190880987906
2224	94-95	0.6199126738543654
2224	96-97	0.47062949613685134
2224	98-99	0.32401282445303536
2224	100	0.7887915427586094
2225	1	1.357396212422863
2225	2	1.7883915487176978
2225	3	0.8416008320850654
2225	4	0.9507888215952569
2225	5	3.259894787597375
2225	6	-0.1448382960834529
2225	7	0.27111815170621867
2225	8	0.4550164899299247
2225	9	0.2232219611594033
2225	10-11	0.29440505438147113
2225	12-13	0.253320854154822
2225	14-15	0.26631984163041267
2225	16-17	0.2538256490678421
2225	18-19	0.09883422148932652
2225	20-21	0.17976039292773294
2225	22-23	0.2908060789700926
2225	24-25	0.23515946593081338
2225	26-27	0.27314258344918585
2225	28-29	0.12521941972774187
2225	30-31	0.2066444308732187
2225	32-33	0.257681589955574
2225	34-35	0.27096269160667674
2225	36-37	0.3585177762988465
2225	38-39	0.23609778077488386
2225	40-41	0.2299724851174858
2225	42-43	0.5214807618545763
2225	44-45	0.41798614266094347
2225	46-47	0.28448754027294854
2225	48-49	0.2838935115879977
2225	50-51	0.3713269590132171
2225	52-53	0.25717405418691186
2225	54-55	0.35428352252058204
2225	56-57	0.4486378311110286
2225	58-59	0.5728278331689509
2225	60-61	0.3519802064908504
2225	62-63	0.3258042936509611
2225	64-65	0.4019223789018369
2225	66-67	0.45105146399937013
2225	68-69	0.6009178172564873
2225	70-71	0.47021005471940924
2225	72-73	0.48425655883172425
2225	74-75	0.4075404495677617
2225	76-77	0.5718363687721038
2225	78-79	0.4326285130911458
2225	80-81	0.4352290268059704
2225	82-83	0.32392407892627517
2225	84-85	0.37834088144931144
2225	86-87	0.2517395060777403
2225	88-89	0.44460352683381643
2225	90-91	0.5449814126609667
2225	92-93	0.5008763848197333
2225	94-95	0.6742484463473986
2225	96-97	0.6941409746968077
2225	98-99	0.5755317363232209
2225	100	0.7269683547079921
2226	1	1.5866254645721405
2226	2	1.3218205920451709
2226	3	0.7585804261647837
2226	4	0.9907443095788437
2226	5	3.1686077553093703
2226	6	-0.22166029686501076
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2226	18-19	0.11272293228944363
2226	20-21	0.14009134801272438
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2226	30-31	0.25512513012097315
2226	32-33	0.06765458660954238
2226	34-35	0.17644899607130782
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2226	60-61	0.4212180679601758
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2226	74-75	0.4651887771615435
2226	76-77	0.642334182859635
2226	78-79	0.4248528774668827
2226	80-81	0.32388441587396244
2226	82-83	0.1729007299618317
2226	84-85	0.0913939997328228
2226	86-87	0.3059085387809404
2226	88-89	0.3903334414733237
2226	90-91	0.26026768781727583
2226	92-93	0.16973400132032168
2226	94-95	0.43918462441988737
2226	96-97	0.4139782066477693
2226	98-99	0.38745404178922627
2226	100	0.48693043706438033
2227	1	1.293705390584602
2227	2	1.4516334327772569
2227	3	0.6691747602769329
2227	4	-0.18411209618439628
2227	5	3.1807463359168473
2227	6	0.05999858163966465
2227	7	0.39221950554990315
2227	8	0.5401736044569887
2227	9	0.18796706091726634
2227	10-11	0.312148459552148
2227	12-13	0.3359890437586728
2227	14-15	0.3148563884707727
2227	16-17	0.29742497598073214
2227	18-19	0.19871580125361277
2227	20-21	0.18502721736630434
2227	22-23	0.2645730750586637
2227	24-25	0.3393635669048294
2227	26-27	0.29653012348563124
2227	28-29	0.1504626970739622
2227	30-31	0.3487765611957414
2227	32-33	0.2607103449818098
2227	34-35	0.3116186319747385
2227	36-37	0.23754744253091786
2227	38-39	0.2572228277960278
2227	40-41	0.3039180245915105
2227	42-43	0.5162066331609054
2227	44-45	0.4054757797490325
2227	46-47	0.2671502060338895
2227	48-49	0.34050514069253524
2227	50-51	0.2535974319272185
2227	52-53	0.32062360734911266
2227	54-55	0.27337291929868
2227	56-57	0.5223519170017568
2227	58-59	0.44155188023756864
2227	60-61	0.5269180290193631
2227	62-63	0.4367584393635511
2227	64-65	0.2981736525813119
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2227	70-71	0.667133456822306
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2227	78-79	0.4092053648111147
2227	80-81	0.5061480938334668
2227	82-83	0.26489839351323496
2227	84-85	0.32878630405525655
2227	86-87	0.36858316397565005
2227	88-89	0.3583952843471607
2227	90-91	0.41557945767709015
2227	92-93	0.3883294549807559
2227	94-95	0.6007007605180164
2227	96-97	0.5054921523253384
2227	98-99	0.591766128043119
2227	100	0.6944630499927342
2228	1	1.4589852718843943
2228	2	1.4398649966894403
2228	3	-0.06613239498146051
2228	4	0.041185885665800726
2228	5	3.0690446994793312
2228	6	0.22680808258163054
2228	7	0.17960914760227809
2228	8	0.46609156094179127
2228	9	0.2884614398959968
2228	10-11	0.21797172676306786
2228	12-13	0.24728372564457857
2228	14-15	0.19817694675577258
2228	16-17	0.11625739812989622
2228	18-19	0.11598920575782046
2228	20-21	0.14637299944553206
2228	22-23	0.25598501033996257
2228	24-25	0.22702210063711448
2228	26-27	0.09509177617670161
2228	28-29	0.16685677807085142
2228	30-31	0.23640446559527817
2228	32-33	0.16187318221231095
2228	34-35	0.20641595506340593
2228	36-37	0.3379162258537818
2228	38-39	0.2543795330033163
2228	40-41	0.22696975276311093
2228	42-43	0.3467014431051254
2228	44-45	0.30635556500509153
2228	46-47	0.3896322901829805
2228	48-49	0.22054343505427454
2228	50-51	0.21373158532630043
2228	52-53	0.2675291212446709
2228	54-55	0.2652661506778955
2228	56-57	0.4090403166422192
2228	58-59	0.36799176670895406
2228	60-61	0.16691347309851068
2228	62-63	0.4130253239756172
2228	64-65	0.33386682978201065
2228	66-67	0.4118383944177779
2228	68-69	0.3769010555211878
2228	70-71	0.4642030454349637
2228	72-73	0.5446408183698637
2228	74-75	0.4113697615983085
2228	76-77	0.5120507095394871
2228	78-79	0.41099340982854216
2228	80-81	0.32820290648660944
2228	82-83	0.28142345723455975
2228	84-85	0.3557861378257101
2228	86-87	0.3574671788501078
2228	88-89	0.43650516207609513
2228	90-91	0.43555332269869496
2228	92-93	0.26748134568791215
2228	94-95	0.429836643935694
2228	96-97	0.6067037810054785
2228	98-99	0.6258827411469312
2228	100	0.7219940487244614
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	19.0
13	44.0
14	131.0
15	536.0
16	1905.0
17	4783.0
18	7683.0
19	10124.0
20	11719.0
21	13585.0
22	15364.0
23	17641.0
24	19997.0
25	22614.0
26	25595.0
27	28777.0
28	32511.0
29	36833.0
30	41944.0
31	49072.0
32	56859.0
33	68306.0
34	83567.0
35	107748.0
36	151488.0
37	249195.0
38	613218.0
39	1168934.0
40	1395.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.65010533951706	19.148497964441315	19.063059659588077	34.13833703645354
2	18.811006221908762	51.602848021634316	12.605356986370502	16.98078877008641
3	40.62233533585282	16.399814610638348	23.307961361028187	19.669888692480644
4	17.432793717032066	16.514292893372613	23.184051728840256	42.868861660755066
5	17.08531183454879	34.01173358408523	26.778697959977997	22.12425662138798
6	17.530872713029726	28.90866969760208	28.889701423887427	24.670756165480768
7	20.159087157986015	25.94419949134058	35.96441002862133	17.93230332205208
8	22.077838897770857	28.39174024937473	32.639718579793616	16.890702273060793
9	21.256502088445647	28.368724941379586	26.742872908695038	23.63190006147973
10-11	19.640415725437933	30.33224039946692	28.392672826839366	21.63467104825578
12-13	24.31069328512553	26.612364851049787	27.233954124930893	21.842987738893793
14-15	21.735494989243687	27.645291170039844	28.508224453448022	22.110989387268454
16-17	20.707055599564608	29.920498650929922	28.305591206603914	21.066854542901556
18-19	21.391585054407976	29.71422307323337	29.696275355989453	19.197916516369197
20-21	22.718326062161744	29.020033523520482	29.851294364733512	18.410346049584263
22-23	21.702573245161947	30.74644556017465	28.500922195941914	19.05005899872149
24-25	22.30190383050035	28.28475777795999	26.578633700111943	22.834704691427714
26-27	19.946758624669947	26.67873621324985	30.24931138831927	23.12519377376093
28-29	19.570266262688612	28.22179331275436	30.544942793139086	21.662997631417944
30-31	20.0238094819106	30.2615523500645	28.2889728487438	21.425665319281105
32-33	20.582213389912045	30.18330953794482	28.797939320527576	20.436537751615557
34-35	21.311154647033508	29.1915046064048	30.216389644237534	19.28095110232416
36-37	21.650999951787504	28.20920140752333	28.86380040449228	21.275998236196887
38-39	21.505717100060775	29.215206560614703	29.171023130579897	20.108053208744625
40-41	20.986235121862467	29.734631899881176	30.00927136959191	19.269861608664446
42-43	20.593141426149238	29.960053941783134	29.401315886713807	20.045488745353822
44-45	20.44593390946679	30.42787357909506	28.757169849101928	20.36902266233622
46-47	19.744454527042564	28.36630765226967	28.92546074218128	22.963777078506485
48-49	20.725988681676824	28.176367642447687	30.365197335151095	20.73244634072439
50-51	21.67283634110094	30.98573086095903	28.14715861242327	19.194274185516754
52-53	20.125135995391243	30.605953056933572	28.175482487805503	21.09342845986968
54-55	22.270824819812542	28.688919558095915	28.017974924227467	21.02228069786408
56-57	21.341359330186148	28.709251893609434	29.465727789750133	20.483660986454282
58-59	21.27020759208179	28.007209637346513	28.982274020421674	21.740308750150025
60-61	19.510941526420577	30.442086407837888	29.87265018699967	20.174321878741864
62-63	20.436120888192484	31.392323845795527	27.379904679395906	20.791650586616083
64-65	19.688364009586905	30.430698084114688	28.89273572543769	20.988202180860714
66-67	20.27810515743491	30.418407038038954	29.454333089220917	19.849154715305215
68-69	20.676245351629213	30.04739956932517	29.446186233256277	19.83016884578934
70-71	19.369457982458393	31.17333729356166	29.217581583812148	20.2396231401678
72-73	19.881777330766223	31.000880845809046	28.851976026072755	20.265365797351972
74-75	19.839670578447887	29.249922666453642	30.488825434519512	20.42158132057896
76-77	19.23066230243874	30.85900590057598	28.650680060121335	21.259651736863944
78-79	19.235958638605823	29.606642344577168	28.587106430315174	22.57029258650184
80-81	21.029148551757437	29.601186944455588	28.211837631291303	21.157826872495672
82-83	20.106493308140838	28.243231687081906	30.039305500764186	21.610969504013074
84-85	20.5531627220986	29.12230032020839	28.607587942934703	21.716949014758303
86-87	21.83565028978525	28.721784692849457	29.489383925250223	19.953181092115074
88-89	21.12249246635771	29.175791555915758	29.389035070895243	20.312680906831286
90-91	20.876450377905023	28.883683659870346	30.071505816995924	20.168360145228707
92-93	20.384031880776483	29.736376186968762	30.176148046848468	19.703443885406287
94-95	20.723278928289016	28.84836888682275	30.515905372596368	19.912446812291865
96-97	20.081225737589595	29.32892781392933	30.083171833204474	20.506674615276605
98-99	22.04157395145741	29.532124126412455	29.06159480600101	19.36470711612912
100	20.62541108190599	31.399038635804573	28.780853797543415	19.194696484746025
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	5.5
2	18.5
3	27.0
4	24.5
5	25.0
6	36.5
7	48.5
8	56.5
9	64.0
10	80.5
11	105.5
12	116.5
13	132.0
14	161.5
15	184.0
16	235.5
17	348.0
18	542.0
19	832.5
20	1279.0
21	1942.0
22	2963.5
23	4650.5
24	7154.0
25	10703.0
26	15424.0
27	20711.0
28	26634.0
29	33744.5
30	41968.0
31	51365.0
32	62120.0
33	74318.0
34	87962.5
35	103263.0
36	118032.0
37	128035.0
38	133765.0
39	137955.0
40	140511.5
41	141068.5
42	139131.0
43	134953.5
44	132256.5
45	128904.5
46	119060.5
47	107913.5
48	100353.0
49	94961.0
50	90656.0
51	83930.0
52	72868.5
53	60692.0
54	49847.0
55	40534.5
56	31891.0
57	23514.0
58	16568.0
59	12028.0
60	9604.0
61	7927.0
62	6304.5
63	4704.0
64	3409.0
65	2903.0
66	3143.5
67	2953.5
68	2042.5
69	1820.5
70	2491.0
71	2455.5
72	1357.0
73	484.0
74	221.0
75	138.0
76	89.5
77	70.5
78	67.0
79	54.5
80	41.0
81	42.5
82	39.0
83	30.0
84	26.5
85	24.0
86	20.0
87	18.5
88	17.0
89	16.5
90	16.0
91	13.0
92	12.0
93	13.0
94	15.0
95	16.0
96	15.0
97	21.0
98	46.0
99	96.0
100	98.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.319027008499125
2	0.006193722029274486
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	4.7508663292730437E-4
30-31	0.009924031887814802
32-33	0.0
34-35	0.0
36-37	0.0
38-39	8.4459845853743E-4
40-41	0.0015308347060990918
42-43	1.759580121952979E-4
44-45	0.0
46-47	0.002023517140245926
48-49	0.0
50-51	0.0
52-53	0.0013548766939037939
54-55	0.018229250063432866
56-57	0.011261312780499065
58-59	0.013724724951233239
60-61	1.583622109757681E-4
62-63	0.01173639941342637
64-65	8.797900609764895E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.759580121952979E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2841587.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.57215988797658
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.77329261470493	63.10526398367272
2	8.832234745041696	12.996131736606994
3	2.4689159567046803	5.449304385499603
4	1.0261879709529085	3.019954618962626
5	0.5424488238097261	1.9954565798187007
6	0.33324627539771473	1.4710588953380006
7	0.22552210756377206	1.16145039891687
8	0.15641979025028144	0.9206513454349972
9	0.11038612370561822	0.7309210988415336
>10	0.4956811629813088	6.226016782536573
>50	0.02481465453614502	1.2331839679117322
>100	0.010023614086366968	1.2864032791111377
>500	7.289649397095745E-4	0.3176058915826997
>1k	9.719532519264619E-5	0.08659703576584502
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	3.5191602439059585E-5	0.0	0.0	0.0	0.0
12-13	3.5191602439059585E-5	0.0	0.0	0.0	0.0
14-15	7.038320487811917E-5	0.0	0.0	0.0	0.0
16-17	7.038320487811917E-5	0.0	0.0	0.0	0.0
18-19	7.038320487811917E-5	0.0	0.0	0.0	0.0
20-21	7.038320487811917E-5	0.0	0.0	0.0	0.0
22-23	7.038320487811917E-5	0.0	0.0	0.0	0.0
24-25	7.038320487811917E-5	0.0	0.0	0.0	0.0
26-27	7.038320487811917E-5	0.0	0.0	0.0	0.0
28-29	7.038320487811917E-5	0.0	0.0	0.0	0.0
30-31	7.038320487811917E-5	0.0	0.0	0.0	0.0
32-33	7.038320487811917E-5	0.0	0.0	0.0	0.0
34-35	7.038320487811917E-5	0.0	0.0	0.0	0.0
36-37	7.038320487811917E-5	0.0	0.0	0.0	0.0
38-39	7.038320487811917E-5	0.0	0.0	0.0	0.0
40-41	7.038320487811917E-5	0.0	0.0	0.0	0.0
42-43	7.038320487811917E-5	0.0	0.0	0.0	0.0
44-45	7.038320487811917E-5	0.0	0.0	0.0	0.0
46-47	7.038320487811917E-5	0.0	0.0	0.0	0.0
48-49	7.038320487811917E-5	0.0	0.0	0.0	0.0
50-51	7.038320487811917E-5	0.0	0.0	0.0	0.0
52-53	7.038320487811917E-5	0.0	0.0	0.0	0.0
54-55	1.4076640975623834E-4	0.0	0.0	0.0	0.0
56-57	2.1114961463435748E-4	0.0	1.2317060853670855E-4	0.0	0.0
58-59	2.639370182929469E-4	0.0	2.463412170734171E-4	0.0	0.0
60-61	3.167244219515362E-4	0.0	2.463412170734171E-4	0.0	0.0
62-63	4.926824341468341E-4	0.0	2.463412170734171E-4	0.0	0.0
64-65	5.982572414640129E-4	0.0	2.463412170734171E-4	0.0	0.0
66-67	6.158530426835427E-4	3.5191602439059585E-5	2.463412170734171E-4	0.0	0.0
68-69	7.56619452439781E-4	7.038320487811917E-5	2.463412170734171E-4	0.0	0.0
70-71	7.918110548788405E-4	7.038320487811917E-5	2.463412170734171E-4	0.0	0.0
72-73	8.270026573179002E-4	7.038320487811917E-5	2.463412170734171E-4	0.0	0.0
74-75	9.149816634155491E-4	7.038320487811917E-5	2.463412170734171E-4	0.0	0.0
76-77	0.0011085354768303767	7.038320487811917E-5	2.463412170734171E-4	0.0	0.0
78-79	0.0012493018865866153	7.038320487811917E-5	2.463412170734171E-4	0.0	0.0
80-81	0.0014780473024405025	7.038320487811917E-5	2.463412170734171E-4	0.0	0.0
82-83	0.00167160111585533	7.038320487811917E-5	2.463412170734171E-4	0.0	0.0
84-85	0.0017947717243920387	7.038320487811917E-5	2.463412170734171E-4	0.0	0.0
86-87	0.001865154929270158	7.038320487811917E-5	2.463412170734171E-4	0.0	0.0
88	0.001865154929270158	7.038320487811917E-5	2.463412170734171E-4	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	11705	0.0	81.72546	8
CACCGGC	11815	0.0	78.62065	9
CTTCACC	13025	0.0	76.542274	6
CCTTCAC	14385	0.0	73.58025	5
CGGGCCC	945	0.0	72.517975	7
CGTAGTG	2690	0.0	71.19221	4
GTGTGGC	4200	0.0	69.95998	7
TTCACCG	14305	0.0	68.8074	7
ATGCAGC	110	0.0	68.27586	2
TGCAGCA	70	0.0	67.05428	3
GGGCCCG	1030	0.0	66.53348	8
ACGGGCC	1045	0.0	66.02761	6
GGCCCGA	1045	0.0	64.680115	9
CGGGGCA	1070	0.0	64.04625	94
GCGTAGT	1875	0.0	63.33501	3
ACCTTCA	6030	0.0	61.41639	4
AACGGGC	1130	0.0	61.060936	5
TGTGGCA	5060	0.0	60.57414	8
AGCGTAG	1560	0.0	60.17904	2
CAGCGTA	1130	0.0	59.938725	1
>>END_MODULE