FastQCFastQC Report
Tue 7 Mar 2017
1SN_29A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_29A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2257274
Sequences flagged as poor quality0
Sequence length93
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA136710.6056420266214912No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG90280.39995144585903175No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG51010.22598054113058494No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA50980.22584763746005135No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA50360.22310096160235754No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT50170.2222592383556449No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG44730.19815937276555706No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC44400.19669743238968773No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG41930.1857550301824236No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT40880.18110340171374853No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA38120.16887626402465983No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA37080.16426893677949597No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT36200.16037042911051116No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA32920.14583962779884055No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA32870.14561812168128457No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA32230.14278284337656835No Hit
CAGCCCAAAGTGGGTGGTAAACTCCATCTAAGGCTAAATACTTCCCCGAG28460.1260812821128494No Hit
CAGCACTTTGAACTTTGTTCACCATTTCTTCGTAGTCGTTGATACGTTGT27840.12333460625515555No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC27340.121119545079596No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC23990.10627863520334706No Hit
CAGCTAAAAATATCAGATTAGTTCCGAAATTTCAGGAAAAAGCTATTGAT23300.10322185078107486No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTTAAAAAAAAGCCATGAGTGCTC22790.10096248838200414No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCG202.699091E-587.03
TGCTGCC550.087.02
AGCTGCG550.087.02
GCAGCAT800.087.03
AGCAGCC1200.087.02
CAGCAGC9100.085.087911
CAGCTGC8850.085.03391
GCTGCCC2000.084.8253
AGCAGCG2000.084.8252
AGCAGCA3550.084.549292
AGCTGCC3000.084.1000062
AGCTGCA2450.083.448982
AGCAGCT2400.083.3752
GCTGCTT1050.082.857153
GCTGCAT950.082.421053
GCAGCTT800.081.56253
CAGCGTA67700.081.40991
GCAGCTA700.080.785723
CAGCCCT129200.080.501931
GCAGCCA650.080.307693
Produced by FastQC (version 0.11.5)