##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1SN_29A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2257274 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.962530911178705 12.0 12.0 12.0 2.0 32.0 2 14.34984543303117 12.0 12.0 12.0 12.0 22.0 3 13.625566944907884 12.0 12.0 12.0 12.0 22.0 4 13.530799982633921 12.0 12.0 12.0 12.0 22.0 5 22.363334269565858 22.0 22.0 22.0 22.0 27.0 6 26.699314305662494 27.0 22.0 32.0 22.0 32.0 7 34.73771859331211 37.0 37.0 37.0 27.0 41.0 8 37.375531282422955 41.0 37.0 41.0 27.0 41.0 9 38.34025864826335 41.0 37.0 41.0 32.0 41.0 10-11 38.72830724138939 41.0 41.0 41.0 34.5 41.0 12-13 38.9141289449132 41.0 41.0 41.0 37.0 41.0 14-15 38.861454347146164 41.0 41.0 41.0 37.0 41.0 16-17 38.795802148963745 41.0 41.0 41.0 34.5 41.0 18-19 38.706041667958786 41.0 41.0 41.0 32.0 41.0 20-21 38.73378708123161 41.0 41.0 41.0 32.0 41.0 22-23 38.70549698441572 41.0 41.0 41.0 34.5 41.0 24-25 38.66618230662294 41.0 41.0 41.0 32.0 41.0 26-27 38.67826325027444 41.0 41.0 41.0 32.0 41.0 28-29 38.59381980211529 41.0 41.0 41.0 32.0 41.0 30-31 38.50435436725891 41.0 41.0 41.0 32.0 41.0 32-33 38.50190672465992 41.0 41.0 41.0 32.0 41.0 34-35 38.46224029515247 41.0 41.0 41.0 32.0 41.0 36-37 38.377868836481525 41.0 41.0 41.0 32.0 41.0 38-39 38.267833679030545 41.0 39.0 41.0 32.0 41.0 40-41 38.32737673849076 41.0 41.0 41.0 32.0 41.0 42-43 38.130271070326415 41.0 39.0 41.0 32.0 41.0 44-45 38.249296718076764 41.0 41.0 41.0 32.0 41.0 46-47 38.150749089388356 41.0 41.0 41.0 32.0 41.0 48-49 38.0966827686847 41.0 37.0 41.0 32.0 41.0 50-51 38.01205148333787 41.0 37.0 41.0 32.0 41.0 52-53 38.062198474797476 41.0 37.0 41.0 32.0 41.0 54-55 37.91333351644506 41.0 37.0 41.0 27.0 41.0 56-57 37.724355793758306 41.0 37.0 41.0 27.0 41.0 58-59 37.6987882286333 41.0 37.0 41.0 27.0 41.0 60-61 37.74172896157046 41.0 37.0 41.0 27.0 41.0 62-63 37.691161994511965 41.0 37.0 41.0 27.0 41.0 64-65 37.64961143396859 41.0 37.0 41.0 27.0 41.0 66-67 37.579603760996676 41.0 37.0 41.0 27.0 41.0 68-69 37.569647947037 41.0 37.0 41.0 27.0 41.0 70-71 37.55366561613698 41.0 37.0 41.0 27.0 41.0 72-73 37.319062284862184 41.0 37.0 41.0 27.0 41.0 74-75 37.30274459370019 41.0 37.0 41.0 27.0 41.0 76-77 36.242677893778065 39.0 34.5 41.0 24.5 41.0 78-79 36.764957422094085 41.0 37.0 41.0 27.0 41.0 80-81 37.460934959601715 41.0 37.0 41.0 27.0 41.0 82-83 37.59971275086676 41.0 37.0 41.0 27.0 41.0 84-85 37.47014695601863 41.0 37.0 41.0 27.0 41.0 86-87 37.30323367920775 41.0 37.0 41.0 27.0 41.0 88-89 37.154790025490925 41.0 37.0 41.0 27.0 41.0 90-91 36.98210562829324 41.0 37.0 41.0 27.0 41.0 92-93 36.96276703670002 41.0 37.0 41.0 27.0 41.0 94-95 36.86383287983648 41.0 37.0 41.0 27.0 41.0 96-97 36.847553066220584 41.0 37.0 41.0 27.0 41.0 98-99 36.73887396922128 41.0 37.0 41.0 27.0 41.0 100 36.63893085199227 41.0 37.0 41.0 22.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.6510804176169422 1101 2 -0.37986435450027045 1101 3 0.022178285214597082 1101 4 -0.09252179398095173 1101 5 -1.0900315790120914 1101 6 -0.7066474917672565 1101 7 -0.656647810389245 1101 8 -0.5310502811105451 1101 9 -0.3192593195849156 1101 10-11 -0.36685384596953696 1101 12-13 -0.3476995409815373 1101 14-15 -0.3545360662247248 1101 16-17 -0.25071118723821684 1101 18-19 -0.11048855657589485 1101 20-21 -0.1779703492354514 1101 22-23 -0.14680224544405007 1101 24-25 -0.21712305865352022 1101 26-27 -0.17526461679651106 1101 28-29 -0.11276404810454466 1101 30-31 -0.19904909428478845 1101 32-33 -0.4406307586518565 1101 34-35 -0.18983617840816436 1101 36-37 -0.33296976679883983 1101 38-39 -0.1891953193641811 1101 40-41 -0.27228809844428525 1101 42-43 -0.35543003341513923 1101 44-45 -0.2694033626547565 1101 46-47 -0.49176228187874926 1101 48-49 -0.38065905310052983 1101 50-51 -0.5000627451152511 1101 52-53 -0.3287772495983958 1101 54-55 -0.34281516462102957 1101 56-57 -0.21911576847438852 1101 58-59 -0.1803798295504535 1101 60-61 -0.22916250299959984 1101 62-63 -0.3828384227526982 1101 64-65 -0.27346267611961395 1101 66-67 -0.1206879819301534 1101 68-69 -0.10705923558611374 1101 70-71 -0.30834661125482654 1101 72-73 -0.041816406260146266 1101 74-75 -0.06011414171534568 1101 76-77 -0.7126856532930788 1101 78-79 -0.2852673933483416 1101 80-81 -0.2879669847210238 1101 82-83 -0.3366684051498652 1101 84-85 -0.3614862353469519 1101 86-87 -0.4445484834461908 1101 88-89 -0.6145221904382652 1101 90-91 -0.5803044535201423 1101 92-93 -0.4095515575832849 1101 94-95 -0.255989384266492 1101 96-97 -0.5600608857625531 1101 98-99 -0.6203878926711184 1101 100 -0.8026761381252854 1102 1 1.587701916972204 1102 2 0.7741830966202095 1102 3 -0.15322288254054328 1102 4 -0.006087660602023703 1102 5 -0.7046108095801209 1102 6 -0.02076817947468257 1102 7 0.11613201474263235 1102 8 -0.09689018650141179 1102 9 -0.14816166639211303 1102 10-11 -0.17544667761799815 1102 12-13 -0.19049187922953337 1102 14-15 -0.1774287841916049 1102 16-17 -0.09561495481077742 1102 18-19 -0.1109264393098286 1102 20-21 -0.007460695460508759 1102 22-23 -0.1302536620875543 1102 24-25 -0.08315753053285135 1102 26-27 -0.0050138506925492266 1102 28-29 -0.125527019458211 1102 30-31 -0.1801384630721259 1102 32-33 -0.2311466155535129 1102 34-35 0.0016206047795819245 1102 36-37 -0.23561922449595585 1102 38-39 -0.14846090274814827 1102 40-41 -0.12777063961951285 1102 42-43 -0.17779365367997713 1102 44-45 -0.07272595373996182 1102 46-47 -0.4204825549588094 1102 48-49 -0.5971481749840848 1102 50-51 -0.2802139355151141 1102 52-53 -0.226232670031969 1102 54-55 -0.19038757429404285 1102 56-57 -0.175731283517095 1102 58-59 -0.24601287923957926 1102 60-61 -0.2622137441846988 1102 62-63 -0.31327893125642703 1102 64-65 -0.34263898841930285 1102 66-67 -0.22336219615836228 1102 68-69 -0.4685871537272348 1102 70-71 -0.21364639172826827 1102 72-73 -0.0701132739610415 1102 74-75 -0.28000404257726075 1102 76-77 -0.11422310257144375 1102 78-79 -0.11088231199527598 1102 80-81 -0.2553290732868163 1102 82-83 -0.40751546525455495 1102 84-85 -0.5072863012965243 1102 86-87 -0.2877393942117479 1102 88-89 -0.596470150327086 1102 90-91 -0.5739671123257821 1102 92-93 -0.5007573660303279 1102 94-95 -0.16074810859017674 1102 96-97 -0.18058957127980335 1102 98-99 -0.17986081518343155 1102 100 -0.5228293792717054 1103 1 1.6378693257929875 1103 2 0.6483996130805654 1103 3 0.4682177565721233 1103 4 1.1159223184314317 1103 5 3.019479883941397 1103 6 0.2340191288613518 1103 7 -0.4711861062067868 1103 8 -0.1461524908327405 1103 9 -0.23161671931350014 1103 10-11 -0.06570137733558568 1103 12-13 -0.362143327988548 1103 14-15 -0.3476022375637271 1103 16-17 -0.3075522335158851 1103 18-19 -0.25910908892233664 1103 20-21 -0.08487040793397682 1103 22-23 -0.16332114563667233 1103 24-25 -0.19884006375223606 1103 26-27 -0.08321740372086595 1103 28-29 -0.19460140610591026 1103 30-31 -0.1283210337740428 1103 32-33 -0.39885135105703995 1103 34-35 -0.43227678146487847 1103 36-37 -0.29332851314063646 1103 38-39 -0.06797959548372035 1103 40-41 -0.2695832316983058 1103 42-43 -0.13632811038448978 1103 44-45 -0.5907747788796129 1103 46-47 -0.538644458976691 1103 48-49 -0.631424174248643 1103 50-51 -0.4265662539477617 1103 52-53 -0.39188928112189814 1103 54-55 -0.361266093805078 1103 56-57 -0.39742980244419357 1103 58-59 -0.38321985462026475 1103 60-61 -0.17251567978260596 1103 62-63 -0.32522283845126054 1103 64-65 -0.29059982035789034 1103 66-67 -0.48203861301777096 1103 68-69 -0.2682252697526337 1103 70-71 -0.32988248275005816 1103 72-73 -0.2412107703646882 1103 74-75 -0.3102891386548521 1103 76-77 -0.5348654821361265 1103 78-79 -0.7025436719246372 1103 80-81 -0.43619017723021614 1103 82-83 -0.5213562252582449 1103 84-85 -0.6290859836024723 1103 86-87 -0.3386177874658429 1103 88-89 -0.3430345804308459 1103 90-91 -0.6423107868921889 1103 92-93 -0.1643334993639769 1103 94-95 -0.30818150213229245 1103 96-97 -0.26391087074787123 1103 98-99 -0.5112340094131724 1103 100 -0.2900912685333168 1104 1 1.7869454083582035 1104 2 -0.48155016658392746 1104 3 -0.03417076526357121 1104 4 0.030051293949018287 1104 5 -1.0454162776421185 1104 6 -0.458624493218192 1104 7 -0.04630347345128172 1104 8 -0.2028490017210629 1104 9 -0.15166611093059856 1104 10-11 -0.1749278878302647 1104 12-13 -0.09618247043074035 1104 14-15 -0.15496262958897233 1104 16-17 0.007437806324752216 1104 18-19 -0.10354750789336009 1104 20-21 -0.04056323724078226 1104 22-23 -0.08594707457821471 1104 24-25 -0.20034910817775398 1104 26-27 -0.2144358994726474 1104 28-29 -0.0022448572622835172 1104 30-31 -0.21107944371203757 1104 32-33 -0.2315580955818035 1104 34-35 -0.1985377904709793 1104 36-37 -0.0642148067597148 1104 38-39 -0.20556795758393775 1104 40-41 -0.15490401305546442 1104 42-43 -0.08615451977552624 1104 44-45 -0.0598382027711466 1104 46-47 -0.517156179126772 1104 48-49 -0.5674355888088058 1104 50-51 -0.6060171969257198 1104 52-53 -0.2617776423934046 1104 54-55 -0.4317316315501003 1104 56-57 -0.2925684366748271 1104 58-59 -0.3556500765303383 1104 60-61 -0.07691485375752904 1104 62-63 -0.2152081390585039 1104 64-65 -0.13063709714776905 1104 66-67 -0.683992109484322 1104 68-69 -0.4646600195729036 1104 70-71 -0.18979566413786841 1104 72-73 -0.18103869216682256 1104 74-75 -0.41770891353350237 1104 76-77 -0.6007791545395946 1104 78-79 -0.39585622514389485 1104 80-81 -0.3956273893800528 1104 82-83 -0.6119856472478702 1104 84-85 -0.5361768090681664 1104 86-87 -0.42701549668098693 1104 88-89 -0.5932336023359426 1104 90-91 -0.5931203929816107 1104 92-93 -0.5384098668011461 1104 94-95 -0.17922100506699223 1104 96-97 -0.45365902840325845 1104 98-99 -0.44587868603689174 1104 100 -0.17211388011507012 1105 1 1.600238143126326 1105 2 0.27130194852882106 1105 3 0.014857686685456173 1105 4 -0.16149393950920832 1105 5 -1.028149117992875 1105 6 0.44175982558599713 1105 7 0.10567051644521541 1105 8 -0.17368649541872827 1105 9 -0.039675364239940336 1105 10-11 -0.004869782781476317 1105 12-13 -0.06430625321523564 1105 14-15 -0.06463349945936869 1105 16-17 -0.041050992821098475 1105 18-19 -0.039165581504626346 1105 20-21 -0.15711020075150373 1105 22-23 0.06795765390240405 1105 24-25 0.009723504596955479 1105 26-27 -0.013086211376396761 1105 28-29 -0.008758875630498153 1105 30-31 -0.09126342243733632 1105 32-33 -0.2133830290983596 1105 34-35 -0.16124477889445643 1105 36-37 -0.18291222552139175 1105 38-39 -0.10360373012652246 1105 40-41 -0.044866372056183934 1105 42-43 0.0027223548599195624 1105 44-45 -0.16349059073789363 1105 46-47 -0.1496842554072657 1105 48-49 -0.1236559925536298 1105 50-51 -0.08137605924320468 1105 52-53 -0.09463455222307715 1105 54-55 -0.16915995794673933 1105 56-57 -0.005053978378981583 1105 58-59 -0.16802131119465713 1105 60-61 -0.05069454887285474 1105 62-63 0.024537345504484165 1105 64-65 -0.24934526694995185 1105 66-67 -0.09528094916282726 1105 68-69 -0.25019035480764984 1105 70-71 -0.09354864229965898 1105 72-73 -0.10385301799007607 1105 74-75 -0.07863419503775759 1105 76-77 -0.18459327769956246 1105 78-79 -0.048994046068635555 1105 80-81 -0.42988798965883745 1105 82-83 -0.4623284447921847 1105 84-85 -0.33447690891690485 1105 86-87 -0.20998102909228322 1105 88-89 -0.06846308144184832 1105 90-91 -0.41683867123337137 1105 92-93 -0.21584639858961907 1105 94-95 -0.14068977020495055 1105 96-97 -0.211619617807429 1105 98-99 -0.3603335217807597 1105 100 -0.08591277881308201 1106 1 1.6066021049549004 1106 2 -0.5978263553611569 1106 3 -0.07307732806708422 1106 4 -0.21528824342387765 1106 5 -1.0770732319145786 1106 6 -0.6394667956502538 1106 7 -0.4161360833103487 1106 8 -0.20750252692373294 1106 9 -0.09862256372638001 1106 10-11 -0.07348267366206329 1106 12-13 -0.17361948852750686 1106 14-15 -0.1390048190401174 1106 16-17 -0.11924377580444201 1106 18-19 -0.030273935516198947 1106 20-21 -0.22458486218378937 1106 22-23 -0.11443065270351838 1106 24-25 -0.2139885801406507 1106 26-27 -0.35743950693334625 1106 28-29 -0.3321845512227597 1106 30-31 -0.12779169916782251 1106 32-33 -0.3059542918910907 1106 34-35 -0.20800539269887963 1106 36-37 -0.5065638186722623 1106 38-39 -0.22479924140388619 1106 40-41 -0.3028463234160057 1106 42-43 -0.10039234459565449 1106 44-45 -0.30256877302743845 1106 46-47 -0.6120807098595691 1106 48-49 -0.5746899048778644 1106 50-51 -0.27433249295552997 1106 52-53 -0.20114603945194176 1106 54-55 -0.4157995885777055 1106 56-57 -0.31441042196748725 1106 58-59 -0.4816171039430728 1106 60-61 -0.1663840912354857 1106 62-63 -0.38733505428317727 1106 64-65 -0.532406388142995 1106 66-67 -0.3898552241896738 1106 68-69 -0.46254113193935353 1106 70-71 -0.7563290331493846 1106 72-73 -0.6182413739769004 1106 74-75 -0.5084003765372103 1106 76-77 -0.6173229296877594 1106 78-79 -0.4875154588558104 1106 80-81 -0.5103800701990622 1106 82-83 -0.577151352585723 1106 84-85 -0.7886039165585998 1106 86-87 -0.802271438089484 1106 88-89 -0.8152076464579636 1106 90-91 -0.8773481585363143 1106 92-93 -0.740085007036221 1106 94-95 -0.7866281922387017 1106 96-97 -0.5381353024117033 1106 98-99 -0.8608575764454116 1106 100 -0.8596483194720719 1107 1 1.4621786525964406 1107 2 -0.516335077572883 1107 3 -0.08843093975547767 1107 4 -0.29820908226763443 1107 5 -1.2138198784288434 1107 6 -0.3026067918716784 1107 7 -0.5169371413114874 1107 8 -0.43027474179872627 1107 9 -0.41733155043839076 1107 10-11 -0.4748927120775761 1107 12-13 -0.5515248981880632 1107 14-15 -0.6711604963497777 1107 16-17 -0.5488180563006964 1107 18-19 -0.404493344798901 1107 20-21 -0.441663393577457 1107 22-23 -0.2954873943119338 1107 24-25 -0.3058665321527414 1107 26-27 -0.25850443545529345 1107 28-29 -0.33644741412344814 1107 30-31 -0.552212632475964 1107 32-33 -0.3830265256589698 1107 34-35 -0.5062628396413871 1107 36-37 -0.501807221909246 1107 38-39 -0.47870439915941176 1107 40-41 -0.5199639000895999 1107 42-43 -0.4563077045049653 1107 44-45 -0.2796309305940383 1107 46-47 -0.5523855149480426 1107 48-49 -0.7913143774517053 1107 50-51 -0.8822567954934755 1107 52-53 -0.5863837748256984 1107 54-55 -0.7410411025269426 1107 56-57 -0.42806745326669216 1107 58-59 -0.653905031394423 1107 60-61 -0.4374225697290939 1107 62-63 -0.5409857879565863 1107 64-65 -0.6359897372203918 1107 66-67 -0.3179413757105607 1107 68-69 -0.7155576946951996 1107 70-71 -0.3912745586862556 1107 72-73 -0.3582149239485588 1107 74-75 -0.44781910567632366 1107 76-77 -0.39815925439334876 1107 78-79 -0.6633861056219885 1107 80-81 -0.44447667577875904 1107 82-83 -0.5584562206504629 1107 84-85 -0.5020021154376266 1107 86-87 -0.22543914386439923 1107 88-89 -0.4338568057249148 1107 90-91 -0.5345564210213496 1107 92-93 -0.5005333979928324 1107 94-95 -0.3428112012443094 1107 96-97 -0.5110485841045076 1107 98-99 -0.48941038203770404 1107 100 -0.8771368263049979 1108 1 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50-51 0.23514565657188058 2223 52-53 0.2788281074950518 2223 54-55 0.3339008287853815 2223 56-57 0.5273561870307475 2223 58-59 0.369933346163954 2223 60-61 0.4475623846366332 2223 62-63 0.3485681898106918 2223 64-65 0.3645555115921226 2223 66-67 0.2885088493522403 2223 68-69 0.40917372208130587 2223 70-71 0.465042742549727 2223 72-73 0.49288887849355945 2223 74-75 0.24753776184748943 2223 76-77 0.3601531476856863 2223 78-79 0.5516924798397085 2223 80-81 0.37665137375991975 2223 82-83 0.28253156876039043 2223 84-85 0.34088664634398214 2223 86-87 0.4537791048735045 2223 88-89 0.482233194523765 2223 90-91 0.41219919311408404 2223 92-93 0.4809229199301228 2223 94-95 0.6712513686992381 2223 96-97 0.7383044536214953 2223 98-99 0.5767116989849086 2223 100 0.534065332308316 2224 1 1.2678917913100438 2224 2 -0.7930346547956013 2224 3 0.07434709589832167 2224 4 -0.07929575788329579 2224 5 -1.0420230122497713 2224 6 0.5971410471969563 2224 7 0.12227049627931308 2224 8 0.0470182290584944 2224 9 0.15561220889219385 2224 10-11 0.270327019201666 2224 12-13 0.08481751377514968 2224 14-15 0.15989167963596884 2224 16-17 0.1978987080103778 2224 18-19 -2.772391171319555E-4 2224 20-21 0.05115265439584249 2224 22-23 0.2833492781673925 2224 24-25 0.3414860289743302 2224 26-27 0.22154712580977787 2224 28-29 0.2597006708334604 2224 30-31 0.12442195998931993 2224 32-33 0.10160257195617106 2224 34-35 0.15966369496487687 2224 36-37 0.2674960110197091 2224 38-39 -0.07537687849099939 2224 40-41 0.15019003098641548 2224 42-43 0.46186820597163347 2224 44-45 0.31209329818241827 2224 46-47 0.2665447456182477 2224 48-49 0.06516804028236578 2224 50-51 0.2812133654629818 2224 52-53 0.3350249391535485 2224 54-55 0.15727581734609686 2224 56-57 0.3615386236863003 2224 58-59 0.33836943511584394 2224 60-61 0.19332011321303355 2224 62-63 0.37182085102674733 2224 64-65 0.39449601756879105 2224 66-67 0.23155580786511365 2224 68-69 0.3382611007031784 2224 70-71 0.2942675873161775 2224 72-73 0.26574676264425534 2224 74-75 0.369729538801856 2224 76-77 0.5643521932111213 2224 78-79 0.3548002174701921 2224 80-81 0.30468755500493927 2224 82-83 0.24187196491990193 2224 84-85 0.11179522746360959 2224 86-87 0.3381865790596592 2224 88-89 0.4608599836767908 2224 90-91 0.33404063584658417 2224 92-93 0.2436341573006615 2224 94-95 0.38795331055457183 2224 96-97 0.37662513721375745 2224 98-99 0.3903265426025655 2224 100 0.6164286790832207 2225 1 1.7108842306720913 2225 2 1.3740431771335402 2225 3 0.8843799826289445 2225 4 0.9932857823216583 2225 5 3.156044089142217 2225 6 -0.1904036633745001 2225 7 0.28567607397786077 2225 8 0.4386292894365056 2225 9 0.19639156015323778 2225 10-11 0.2075221743962743 2225 12-13 0.2615888020432138 2225 14-15 0.2932460093327549 2225 16-17 0.30762458377291324 2225 18-19 0.2240155209343513 2225 20-21 0.08622039444074403 2225 22-23 0.33545376606706157 2225 24-25 0.1884943392506031 2225 26-27 0.2572627675488519 2225 28-29 0.1949540680580455 2225 30-31 0.13775018864264155 2225 32-33 0.12280881790374565 2225 34-35 0.17011725535749633 2225 36-37 0.19403607967490188 2225 38-39 0.17278748117184506 2225 40-41 0.13016641251142858 2225 42-43 0.4132794731329952 2225 44-45 0.2876872161817232 2225 46-47 0.4014703951263101 2225 48-49 0.3141280388025578 2225 50-51 0.35498848564850505 2225 52-53 0.28398527841843446 2225 54-55 0.3800697875280079 2225 56-57 0.28938958585196417 2225 58-59 0.3802476880107122 2225 60-61 0.29235609779969707 2225 62-63 0.48461927036079544 2225 64-65 0.24643173948996377 2225 66-67 0.40024755269212164 2225 68-69 0.449939930332782 2225 70-71 0.2599838034534585 2225 72-73 0.4194605877273574 2225 74-75 0.3300524966215548 2225 76-77 0.5728250533831343 2225 78-79 0.31495377649982714 2225 80-81 0.2310226900664034 2225 82-83 0.3657830422378012 2225 84-85 0.3126449960492792 2225 86-87 0.535065941808675 2225 88-89 0.400455198453038 2225 90-91 0.7231029259235129 2225 92-93 0.568349246059789 2225 94-95 0.7132454747896091 2225 96-97 0.5408584811263992 2225 98-99 0.6282834082186923 2225 100 0.47659971344780416 2226 1 1.34431923498623 2226 2 1.0512074205565174 2226 3 0.8097751413882772 2226 4 0.8600057798646628 2226 5 3.149359334126732 2226 6 -0.2600969132595701 2226 7 0.41066323713675246 2226 8 0.41908801815254293 2226 9 0.37131475505344014 2226 10-11 0.4682793272760932 2226 12-13 0.35636080054982244 2226 14-15 0.3607757116236101 2226 16-17 0.3241038485554384 2226 18-19 0.4153619544040126 2226 20-21 0.3832386241947674 2226 22-23 0.4810222245807978 2226 24-25 0.34930936588884975 2226 26-27 0.2680866240142592 2226 28-29 0.4528180128984971 2226 30-31 0.49750050128569967 2226 32-33 0.42600960625374995 2226 34-35 0.39533975260537346 2226 36-37 0.346327683756968 2226 38-39 0.3752722137288629 2226 40-41 0.4547203862695639 2226 42-43 0.641592649826471 2226 44-45 0.4848248645492106 2226 46-47 0.48806268649525464 2226 48-49 0.5619816469866521 2226 50-51 0.6160795163676624 2226 52-53 0.5983619166153105 2226 54-55 0.7585255371371531 2226 56-57 0.5986887135226198 2226 58-59 0.624795629653228 2226 60-61 0.5684213636590698 2226 62-63 0.44013409727218544 2226 64-65 0.535656327103446 2226 66-67 0.48219998583736867 2226 68-69 0.5733467223893953 2226 70-71 0.5743590020045986 2226 72-73 0.4338078571512938 2226 74-75 0.4296765811268415 2226 76-77 0.6444903779800555 2226 78-79 0.5199553697077661 2226 80-81 0.48396160685776124 2226 82-83 0.5266710109264636 2226 84-85 0.5055278914263894 2226 86-87 0.753782440052781 2226 88-89 0.6052164907942199 2226 90-91 0.8062165533220806 2226 92-93 0.64238782664588 2226 94-95 0.6504933568308005 2226 96-97 0.5577979526612467 2226 98-99 0.5236632731415369 2226 100 0.6479093975607384 2227 1 1.6080473305357632 2227 2 1.4820787254753736 2227 3 0.5886068217632587 2227 4 -0.10230560830822277 2227 5 3.1163134749670576 2227 6 0.3052627512659818 2227 7 0.3021365949033381 2227 8 0.19044236969273953 2227 9 0.23461244991270291 2227 10-11 0.1395727771670039 2227 12-13 0.07243256916324015 2227 14-15 0.1304081458530959 2227 16-17 0.08273999035731805 2227 18-19 0.0404789124283198 2227 20-21 0.13100235773444524 2227 22-23 0.17884442805908662 2227 24-25 0.17209548689415755 2227 26-27 0.12674543758625134 2227 28-29 0.11415766045288933 2227 30-31 0.060711549817568766 2227 32-33 0.12978417772220752 2227 34-35 0.12963737415037713 2227 36-37 0.22260127709702715 2227 38-39 0.14046877216867415 2227 40-41 0.04123157830865409 2227 42-43 0.3102550129701598 2227 44-45 0.27293225082802053 2227 46-47 0.2140767642853092 2227 48-49 0.027935157033972757 2227 50-51 0.19711524606112363 2227 52-53 0.17013536925490058 2227 54-55 0.40389428972764563 2227 56-57 0.49794015797085933 2227 58-59 0.4198251646545046 2227 60-61 0.2473385289474095 2227 62-63 0.3383515202734628 2227 64-65 0.2533106433414858 2227 66-67 0.13061079637613915 2227 68-69 -0.022770815616070195 2227 70-71 0.38077075764405066 2227 72-73 0.24960734675821072 2227 74-75 0.24606318973248165 2227 76-77 0.4809982430518147 2227 78-79 0.4421611950219031 2227 80-81 0.3097510720096466 2227 82-83 0.4328846486857785 2227 84-85 0.24572924674819063 2227 86-87 0.30616705296194624 2227 88-89 0.35801797875919306 2227 90-91 0.42625159899106535 2227 92-93 0.07760366697854693 2227 94-95 0.38807148914634837 2227 96-97 0.23337157627066318 2227 98-99 0.4046207525633392 2227 100 0.5068394497058151 2228 1 1.7162418942894782 2228 2 1.508536362300534 2228 3 -0.2094851327934517 2228 4 0.08490998418806228 2228 5 3.157411084783181 2228 6 0.315857656308701 2228 7 0.19868143068218558 2228 8 0.31469410709367907 2228 9 0.20983320430844543 2228 10-11 0.2875221168385593 2228 12-13 0.18801277981826559 2228 14-15 0.3037795501296543 2228 16-17 0.20465479773411488 2228 18-19 0.19215444990046393 2228 20-21 0.11828963253013569 2228 22-23 0.3708205813605332 2228 24-25 0.26336828786308075 2228 26-27 0.2961866493943859 2228 28-29 0.16251310308383182 2228 30-31 0.22430499509523827 2228 32-33 0.0810751604565425 2228 34-35 0.16493993923519668 2228 36-37 0.3447547836287299 2228 38-39 0.19916953774723822 2228 40-41 0.2282596214689434 2228 42-43 0.5115698172513632 2228 44-45 0.3084386896591269 2228 46-47 0.23139551254404012 2228 48-49 0.22156660553880414 2228 50-51 0.2100106837391209 2228 52-53 0.37781729815689147 2228 54-55 0.39100671195090086 2228 56-57 0.4339707192016675 2228 58-59 0.356081123668865 2228 60-61 0.30703859027882174 2228 62-63 0.2469490913946828 2228 64-65 0.20963365577802762 2228 66-67 0.49950534600621666 2228 68-69 0.34719509901524503 2228 70-71 0.47663918241342884 2228 72-73 0.4529711668346721 2228 74-75 0.21935318961639894 2228 76-77 0.4260471340560201 2228 78-79 0.5281178006280172 2228 80-81 0.36484245001237525 2228 82-83 0.3466758007102939 2228 84-85 0.37956063179338884 2228 86-87 0.5142497159061676 2228 88-89 0.4021977581595735 2228 90-91 0.642008427673268 2228 92-93 0.5954570094438765 2228 94-95 0.7322687779882884 2228 96-97 0.6989551243606869 2228 98-99 0.6812446565303389 2228 100 0.7459112848500666 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 19.0 13 17.0 14 132.0 15 383.0 16 1565.0 17 4196.0 18 6773.0 19 8803.0 20 10422.0 21 11977.0 22 13409.0 23 15324.0 24 17376.0 25 19502.0 26 22084.0 27 24471.0 28 27614.0 29 31332.0 30 35380.0 31 40930.0 32 47803.0 33 56985.0 34 70665.0 35 92241.0 36 129987.0 37 215126.0 38 512433.0 39 839767.0 40 558.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.20448247950037 24.298598270146215 18.13809768016231 25.358821570191107 2 22.435258158825462 47.16898292852469 10.498649407196753 19.8971095054531 3 32.203888406990025 23.417626748015525 22.449379206954937 21.929105638039513 4 18.87644122955388 18.754036948992457 25.653243691284267 36.7162781301694 5 18.330694457119517 36.52334630177816 24.793489846602583 20.352469394499735 6 18.428024245173603 27.53378632811081 29.046850315912025 24.991339110803562 7 20.158961650202855 24.895869974136943 36.52489684460106 18.420271531059146 8 21.79877143846959 27.659779007776635 33.87360152112681 16.667848032626964 9 21.179174526442072 27.59142221989887 27.228417994448172 24.00098525921089 10-11 20.076472772025017 30.14058107256806 27.823693534768047 21.95925262063888 12-13 25.32297807000834 26.07133648817113 26.51846873706958 22.08721670475095 14-15 21.88500377003412 26.993134196380236 28.619343509029033 22.50251852455661 16-17 21.575293916467388 29.218849816194226 27.734758828569326 21.471097438769064 18-19 21.79593616016487 29.3147398144842 29.296598463456363 19.592725561894568 20-21 23.189165338368316 28.78445416905524 29.46175342470608 18.564627067870358 22-23 22.212899275852198 30.451088348158002 28.350789492104195 18.98522288388561 24-25 22.76549058731904 27.7154656457302 26.37338223007043 23.145661536880326 26-27 20.58892717499072 25.94450208525859 29.690037629459248 23.77653311029144 28-29 19.88117030174583 27.77776547182784 30.44216361045939 21.89890061596694 30-31 20.47769465299483 29.72432803282694 27.77268632020978 22.02529099396845 32-33 21.031097686855915 29.829475730460725 28.360380706994366 20.779045875688993 34-35 21.801385210656747 29.146417315753425 29.33347923202943 19.718718241560396 36-37 22.133267826590835 27.582872083761213 28.35599488586676 21.9278652037812 38-39 21.72198189884681 29.166872484089666 28.479778244371108 20.631367372692416 40-41 21.470063129914717 29.280983497618784 29.637102669177096 19.611850703289402 42-43 20.859374186655963 29.56829941294143 28.982747083258296 20.589579317144313 44-45 20.814863414897793 30.112117536462122 28.33632514262779 20.736693906012295 46-47 20.130452532418758 27.764114212197793 28.526113654431924 23.579319600951525 48-49 21.23293876439873 27.74451124332076 29.918350611921863 21.104199380358647 50-51 22.154532413876208 30.38370618719748 27.883345132225863 19.578416266700454 52-53 20.59834457454653 30.562395876919844 27.599978646531202 21.23928090200242 54-55 22.81116792655152 27.861969496902724 27.96503487207664 21.361827704469118 56-57 21.963668586619406 28.117301727957468 29.206911829862648 20.71211785556048 58-59 21.650380733713252 27.373836819293935 28.567434573439115 22.408347873553694 60-61 19.65199982811087 30.172677691493572 29.2839710287077 20.89135145168786 62-63 20.88985674520407 30.741041452286616 26.975255788399828 21.393846014109485 64-65 20.194269725341275 30.064272215070037 28.43283535804692 21.308622701541772 66-67 20.76276517604863 29.983643988279667 28.932774665370708 20.320816170300994 68-69 21.466357207853367 29.512079614614795 29.01386805500794 20.007695122523895 70-71 19.987072902979435 30.56441087789963 28.653455451132647 20.79506076798829 72-73 20.430395246655923 30.7003048810202 27.939563384861565 20.92973648746231 74-75 20.20775944790043 28.957982061548577 29.997798229191492 20.8364602613595 76-77 19.738365834187608 30.22725641636771 28.388179724747637 21.646198024697046 78-79 19.776974350477612 29.025630029850163 28.240058583937973 22.957337035734252 80-81 21.05209879726342 29.077371815233366 28.069472446571496 21.80105694093172 82-83 20.553995660252145 27.670632807536876 29.72264332996349 22.05272820224749 84-85 21.414502625733515 28.64884812388749 27.852622233720854 22.084027016658144 86-87 22.026413275481843 28.88105298692139 28.53913614386202 20.553397593734744 88-89 22.116433361656583 28.364965883627775 28.590171153346912 20.92842960136873 90-91 21.95185431631251 28.721169871269502 28.743674892813193 20.583300919604795 92-93 21.342956149762944 28.676004773899844 29.537442065074952 20.443597011262256 94-95 21.493934719489083 28.459571146435923 29.580702209833632 20.465791924241366 96-97 20.908383297729916 28.996346921109268 29.03378145497622 21.061488326184595 98-99 22.803390284032865 29.435327744881658 28.107199214627908 19.654082756457566 100 21.36767623248219 30.673945653031044 27.903701544429254 20.054676570057513 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 9.0 2 16.5 3 17.5 4 19.5 5 23.0 6 27.0 7 29.0 8 36.0 9 43.5 10 49.5 11 63.0 12 78.0 13 86.0 14 100.5 15 128.5 16 170.5 17 238.5 18 352.5 19 588.5 20 933.5 21 1438.5 22 2224.5 23 3420.5 24 5168.0 25 7645.5 26 11246.5 27 15500.0 28 19632.0 29 24561.0 30 30590.5 31 37265.5 32 44862.5 33 53607.5 34 63531.0 35 75235.0 36 86448.5 37 94393.0 38 100037.0 39 104462.0 40 107177.0 41 107755.0 42 106889.5 43 105389.0 44 104109.0 45 101285.0 46 94010.0 47 85787.0 48 81062.5 49 78751.5 50 75886.5 51 71031.0 52 62807.0 53 52944.0 54 44691.5 55 37270.5 56 29630.0 57 22148.5 58 16327.0 59 12730.0 60 10831.0 61 9714.5 62 8524.5 63 7039.0 64 5974.0 65 5567.5 66 5382.5 67 4740.5 68 3694.5 69 3205.5 70 3060.5 71 2472.5 72 1620.5 73 1009.5 74 665.0 75 450.5 76 307.5 77 224.5 78 160.5 79 103.5 80 72.5 81 55.0 82 36.0 83 25.0 84 20.5 85 18.0 86 14.5 87 14.5 88 12.0 89 9.0 90 11.0 91 12.0 92 10.5 93 9.0 94 7.5 95 9.5 96 10.5 97 13.0 98 35.5 99 74.5 100 71.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.282502700159574 2 0.0051832431508093395 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 4.4301223511190933E-4 30-31 0.009989925901773555 32-33 0.0 34-35 0.0 36-37 0.0 38-39 6.423677409122685E-4 40-41 0.0010632293642685825 42-43 1.5505428228916826E-4 44-45 0.0 46-47 0.002414416681359906 48-49 2.2150611755595464E-5 50-51 0.0 52-53 0.0013068860935801325 54-55 0.020112755474080684 56-57 0.012138535242066316 58-59 0.013999186629536333 60-61 2.2150611755595467E-4 62-63 0.012470794418400247 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.2150611755595467E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2257274.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.54261912259504 #Duplication Level Percentage of deduplicated Percentage of total 1 87.65289438312712 67.96835004143877 2 8.033414254058863 12.458639635130249 3 2.056231002333447 4.78336612326043 4 0.8310372202727491 2.5776321059323966 5 0.4118771999132981 1.5969018419078904 6 0.23245844858646902 1.0815262164341948 7 0.1673170448851408 0.908194131897263 8 0.11073356583447651 0.6869256575671693 9 0.08571930820071365 0.5982209700534249 >10 0.3920412589346314 5.248973219594934 >50 0.01898700176669519 0.997694736459683 >100 0.007000633890278017 0.9538490029128655 >500 2.886781961221298E-4 0.13972631741068325 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 4.4301223511190935E-5 0.0 0.0 0.0 0.0 9 4.4301223511190935E-5 0.0 0.0 0.0 0.0 10-11 6.64518352667864E-5 0.0 0.0 0.0 0.0 12-13 8.860244702238187E-5 0.0 0.0 0.0 0.0 14-15 8.860244702238187E-5 0.0 0.0 0.0 0.0 16-17 8.860244702238187E-5 0.0 0.0 0.0 0.0 18-19 8.860244702238187E-5 0.0 0.0 0.0 0.0 20-21 8.860244702238187E-5 0.0 0.0 0.0 0.0 22-23 8.860244702238187E-5 0.0 0.0 0.0 0.0 24-25 8.860244702238187E-5 0.0 0.0 0.0 0.0 26-27 8.860244702238187E-5 0.0 0.0 0.0 0.0 28-29 8.860244702238187E-5 0.0 0.0 0.0 0.0 30-31 8.860244702238187E-5 0.0 0.0 0.0 0.0 32-33 8.860244702238187E-5 0.0 0.0 0.0 0.0 34-35 8.860244702238187E-5 0.0 0.0 0.0 0.0 36-37 8.860244702238187E-5 0.0 0.0 0.0 0.0 38-39 1.1075305877797733E-4 0.0 0.0 0.0 0.0 40-41 1.3290367053357278E-4 0.0 0.0 0.0 0.0 42-43 1.3290367053357278E-4 0.0 0.0 0.0 0.0 44-45 1.3290367053357278E-4 0.0 0.0 0.0 0.0 46-47 1.5505428228916826E-4 0.0 0.0 0.0 0.0 48-49 1.7720489404476374E-4 0.0 0.0 0.0 0.0 50-51 1.7720489404476374E-4 0.0 0.0 4.4301223511190935E-5 0.0 52-53 1.7720489404476374E-4 0.0 0.0 4.4301223511190935E-5 0.0 54-55 1.7720489404476374E-4 0.0 0.0 4.4301223511190935E-5 4.4301223511190935E-5 56-57 2.2150611755595467E-4 0.0 0.0 4.4301223511190935E-5 8.860244702238187E-5 58-59 2.658073410671456E-4 0.0 0.0 4.4301223511190935E-5 8.860244702238187E-5 60-61 3.544097880895275E-4 0.0 0.0 4.4301223511190935E-5 8.860244702238187E-5 62-63 3.987110116007184E-4 0.0 0.0 4.4301223511190935E-5 8.860244702238187E-5 64-65 4.4301223511190933E-4 0.0 0.0 4.4301223511190935E-5 8.860244702238187E-5 66-67 4.4301223511190933E-4 2.2150611755595467E-5 0.0 4.4301223511190935E-5 8.860244702238187E-5 68-69 4.8731345862310023E-4 4.4301223511190935E-5 0.0 4.4301223511190935E-5 8.860244702238187E-5 70-71 5.759159056454821E-4 4.4301223511190935E-5 0.0 4.4301223511190935E-5 8.860244702238187E-5 72-73 6.423677409122685E-4 4.4301223511190935E-5 0.0 4.4301223511190935E-5 8.860244702238187E-5 74-75 7.752714114458413E-4 4.4301223511190935E-5 0.0 4.4301223511190935E-5 8.860244702238187E-5 76-77 8.860244702238187E-4 4.4301223511190935E-5 0.0 4.4301223511190935E-5 8.860244702238187E-5 78-79 0.0011739824230465597 4.4301223511190935E-5 0.0 4.4301223511190935E-5 8.860244702238187E-5 80-81 0.0014840909876248963 4.4301223511190935E-5 0.0 4.4301223511190935E-5 8.860244702238187E-5 82-83 0.0016834464934252554 4.4301223511190935E-5 0.0 4.4301223511190935E-5 8.860244702238187E-5 84-85 0.0017498983286920418 4.4301223511190935E-5 0.0 4.4301223511190935E-5 8.860244702238187E-5 86-87 0.0018163501639588283 1.3290367053357278E-4 0.0 4.4301223511190935E-5 8.860244702238187E-5 88 0.0018163501639588283 1.3290367053357278E-4 0.0 4.4301223511190935E-5 8.860244702238187E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 9345 0.0 77.85395 8 CACCGGC 9260 0.0 75.6793 9 CTTCACC 10225 0.0 74.55057 6 CGTAGTG 2395 0.0 72.514435 4 CCTTCAC 11405 0.0 71.11754 5 GTGTGGC 3805 0.0 67.847885 7 ACCTTCA 4535 0.0 67.17314 4 TTCACCG 11280 0.0 66.45445 7 GCGTAGT 1545 0.0 63.799763 3 TAGCAGC 25 0.003274827 63.497997 1 CAGCTGC 100 0.0 63.497997 1 TGTGGCA 4380 0.0 63.441856 8 AACGGGC 675 0.0 61.88915 5 CGGGGCA 985 0.0 60.51956 94 CGGGCCC 700 0.0 60.34937 7 AGCGTAG 1310 0.0 60.195805 2 GCCTTCA 3250 0.0 60.08115 4 TGTGTGG 4020 0.0 59.548714 6 TAGTGTG 2700 0.0 59.28146 6 GTAGTGT 2655 0.0 59.225475 5 >>END_MODULE