FastQCFastQC Report
Tue 7 Mar 2017
1SN_29A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_29A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2257274
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG93450.077.853958
CACCGGC92600.075.67939
CTTCACC102250.074.550576
CGTAGTG23950.072.5144354
CCTTCAC114050.071.117545
GTGTGGC38050.067.8478857
ACCTTCA45350.067.173144
TTCACCG112800.066.454457
GCGTAGT15450.063.7997633
TAGCAGC250.00327482763.4979971
CAGCTGC1000.063.4979971
TGTGGCA43800.063.4418568
AACGGGC6750.061.889155
CGGGGCA9850.060.5195694
CGGGCCC7000.060.349377
AGCGTAG13100.060.1958052
GCCTTCA32500.060.081154
TGTGTGG40200.059.5487146
TAGTGTG27000.059.281466
GTAGTGT26550.059.2254755
Produced by FastQC (version 0.11.5)