FastQCFastQC Report
Tue 7 Mar 2017
1SN_2A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_2A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3080107
Sequences flagged as poor quality0
Sequence length94
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA170740.5543313917341184No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG86510.2808668659887465No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA73530.23872547284883283No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT72720.23609569407816028No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA66340.2153821279585417No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG60560.1966165461134954No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG56890.1847013756340283No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT56890.1847013756340283No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG50250.16314368299542842No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC48460.15733219657628777No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC45430.14749487598969777No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA44370.14405343710462007No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA43840.14233271766208122No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT42490.13794975304429358No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA40090.13015781594600445No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG38820.12603458256482647No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC38210.12405413188567799No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA32830.10658720622367988No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA32280.10480155397198865No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC31770.1031457673386022No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG31480.10220424160589227No Hit
CTGCAGGTGGAGATAGAGGTGATGCAGGTGGAGATAGCGGTGATGCAGGT30940.1004510557587772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTT450.087.9998863
GCTGCTC202.5504523E-587.9998863
GCTGCAT401.8189894E-1187.9998863
AGCTGCA1300.087.9998862
GCAGCTT202.5504523E-587.9998863
GCAGCTC202.5504523E-587.9998863
GCAGCCA401.8189894E-1187.9998863
CAGCTGC5000.087.119891
CAGCAGC4150.085.87941
AGCTGCC1500.085.066552
AGCTGCG1150.084.173812
AGCAGCA1850.083.243132
AGCAGCG850.082.8234252
CAGCGTA66650.082.454511
GCTGCCC1550.082.322483
CAGCCCT186150.082.019771
AGCCCTT156800.080.114692
GCTGCAA501.8189894E-1279.19993
AGCAGCC950.078.736742
GCCCTTC144350.078.0019763
Produced by FastQC (version 0.11.5)