FastQCFastQC Report
Tue 7 Mar 2017
1SN_2A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_2A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3080107
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG126350.078.273588
CACCGGC125200.076.480689
CTTCACC137150.074.710886
CCTTCAC149500.071.4903955
GTGTGGC47000.071.306037
CGTAGTG26650.070.979644
CGGGGCA14500.070.5690894
TGCAGCC900.067.799543
TTCACCG152050.066.617897
GCGTAGT19050.064.801743
TGTGGCA53350.062.37898
GGCGCGC3500.061.6901326
CAGCGTA12750.061.454311
GCCTTCA43650.060.6485984
GCAGCCT704.947651E-1060.505211
ACCTTCA59100.060.360394
ATCGGAA44050.057.96674394
TGTGTGG53950.057.8570256
CGGGCCC7600.057.437467
GCAGCCC1150.057.142094
Produced by FastQC (version 0.11.5)