##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1SN_2A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3080107
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.817816394040856	12.0	12.0	12.0	2.0	32.0
2	14.56162204754575	12.0	12.0	12.0	12.0	22.0
3	13.720648016448779	12.0	12.0	12.0	12.0	22.0
4	13.797413206748987	12.0	12.0	12.0	12.0	22.0
5	22.432278813690562	22.0	22.0	22.0	22.0	27.0
6	26.82399475083171	27.0	22.0	32.0	22.0	32.0
7	34.935273677180696	37.0	37.0	37.0	27.0	41.0
8	37.55533103233102	41.0	37.0	41.0	27.0	41.0
9	38.52007186763317	41.0	37.0	41.0	32.0	41.0
10-11	38.88015773477999	41.0	41.0	41.0	37.0	41.0
12-13	39.057914221811124	41.0	41.0	41.0	37.0	41.0
14-15	39.01592882974521	41.0	41.0	41.0	37.0	41.0
16-17	38.9193469577518	41.0	41.0	41.0	37.0	41.0
18-19	38.850192055016265	41.0	41.0	41.0	37.0	41.0
20-21	38.854114483685144	41.0	41.0	41.0	37.0	41.0
22-23	38.850609410647095	41.0	41.0	41.0	34.5	41.0
24-25	38.87506375590199	41.0	41.0	41.0	37.0	41.0
26-27	38.83407946542117	41.0	41.0	41.0	37.0	41.0
28-29	38.786842794747066	41.0	41.0	41.0	34.5	41.0
30-31	38.668167696771576	41.0	41.0	41.0	32.0	41.0
32-33	38.70888381475059	41.0	41.0	41.0	32.0	41.0
34-35	38.68586204959763	41.0	41.0	41.0	32.0	41.0
36-37	38.608682425643	41.0	41.0	41.0	32.0	41.0
38-39	38.506710643493875	41.0	41.0	41.0	32.0	41.0
40-41	38.561147875706915	41.0	41.0	41.0	32.0	41.0
42-43	38.29954527553751	41.0	41.0	41.0	32.0	41.0
44-45	38.40773177685061	41.0	41.0	41.0	32.0	41.0
46-47	38.42548862750547	41.0	41.0	41.0	32.0	41.0
48-49	38.36803964927192	41.0	41.0	41.0	32.0	41.0
50-51	38.22551732780712	41.0	41.0	41.0	32.0	41.0
52-53	38.211209545642404	41.0	41.0	41.0	32.0	41.0
54-55	38.0846891033331	41.0	37.0	41.0	32.0	41.0
56-57	37.85108926410673	41.0	37.0	41.0	27.0	41.0
58-59	37.864762490394	41.0	37.0	41.0	29.5	41.0
60-61	37.862103491859216	41.0	37.0	41.0	29.5	41.0
62-63	37.87463422536945	41.0	37.0	41.0	29.5	41.0
64-65	37.83541091267284	41.0	37.0	41.0	27.0	41.0
66-67	37.72265655056788	41.0	37.0	41.0	27.0	41.0
68-69	37.7052578043555	41.0	37.0	41.0	27.0	41.0
70-71	37.688822985694976	41.0	37.0	41.0	27.0	41.0
72-73	37.56901756984416	41.0	37.0	41.0	27.0	41.0
74-75	37.44068469049939	41.0	37.0	41.0	27.0	41.0
76-77	36.411504697726414	39.0	34.5	41.0	24.5	41.0
78-79	36.92971640270939	41.0	37.0	41.0	27.0	41.0
80-81	37.58827128408201	41.0	37.0	41.0	27.0	41.0
82-83	37.782452200524204	41.0	37.0	41.0	27.0	41.0
84-85	37.701748023688786	41.0	37.0	41.0	27.0	41.0
86-87	37.54779330718056	41.0	37.0	41.0	27.0	41.0
88-89	37.44151096049585	41.0	37.0	41.0	27.0	41.0
90-91	37.29563161279786	41.0	37.0	41.0	27.0	41.0
92-93	37.2018119825058	41.0	37.0	41.0	27.0	41.0
94-95	37.03710065916542	41.0	37.0	41.0	27.0	41.0
96-97	36.974641465377665	41.0	37.0	41.0	27.0	41.0
98-99	36.827314440699624	41.0	37.0	41.0	27.0	41.0
100	36.731722631713765	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.4082182802532301
1101	2	-0.5567327027353564
1101	3	0.04716534536128236
1101	4	0.17424131906996898
1101	5	-1.1006444939988285
1101	6	-0.6810257620703801
1101	7	-0.3754293087463978
1101	8	-0.17503858800487393
1101	9	-0.09576528996396405
1101	10-11	-0.0918958873407334
1101	12-13	-0.18897734951112
1101	14-15	-0.11840939671525064
1101	16-17	-0.17683697940846343
1101	18-19	0.0229965272748629
1101	20-21	-0.0631982926244774
1101	22-23	0.08799291492043437
1101	24-25	-0.0767418549136849
1101	26-27	0.05917133410538611
1101	28-29	-0.008436792564978646
1101	30-31	-0.10927461234005165
1101	32-33	-0.11757175971723655
1101	34-35	0.0346451907341887
1101	36-37	0.17589591381047143
1101	38-39	0.03054389187954598
1101	40-41	0.12793488875815484
1101	42-43	0.23235324853767736
1101	44-45	0.025833150167748897
1101	46-47	-0.08231059076886993
1101	48-49	0.06745262641901917
1101	50-51	-0.10016832084443905
1101	52-53	0.04541303002523733
1101	54-55	0.07984681255062043
1101	56-57	0.25713291036306174
1101	58-59	0.14769061044196974
1101	60-61	-0.04922097966954908
1101	62-63	-0.04643009306089141
1101	64-65	-0.030977980780470205
1101	66-67	0.008106593421828734
1101	68-69	0.053664160414882645
1101	70-71	-0.051471389718905414
1101	72-73	0.07293634891500034
1101	74-75	0.08934197995197479
1101	76-77	-0.3953019589792248
1101	78-79	-0.03968426660358659
1101	80-81	0.014001641228539086
1101	82-83	-0.1732523381668969
1101	84-85	-0.04756238895606657
1101	86-87	-0.0750989870219243
1101	88-89	-0.2630220086726851
1101	90-91	-0.2354968766313874
1101	92-93	0.06940825764921499
1101	94-95	0.14958759936166643
1101	96-97	-0.18897610372248153
1101	98-99	-0.20376694281035412
1101	100	-0.17718924405574654
1102	1	1.6040946465214674
1102	2	0.8051632342314647
1102	3	-0.19151856333675354
1102	4	-0.03552625872000981
1102	5	-0.7446814425915989
1102	6	-0.09081845118093668
1102	7	0.0012345850999295749
1102	8	-0.19538305940081813
1102	9	-0.1283528744323732
1102	10-11	-0.18916878179414454
1102	12-13	-0.22198730961799384
1102	14-15	-0.1281519976135641
1102	16-17	-0.2400270366160271
1102	18-19	-0.04772007023260727
1102	20-21	-0.04714909379389809
1102	22-23	-0.1245157355799904
1102	24-25	-0.10901541344590981
1102	26-27	-0.004192338040780896
1102	28-29	-0.21138297690788477
1102	30-31	-0.21381957316477695
1102	32-33	-0.22225498067695781
1102	34-35	-0.14518668171536575
1102	36-37	-0.16407271854743044
1102	38-39	-0.23125540414312695
1102	40-41	-0.15866681020818874
1102	42-43	-0.20074450709098812
1102	44-45	-0.21357909437026734
1102	46-47	-0.4061809577436719
1102	48-49	-0.26063047376977266
1102	50-51	-0.24356377467375268
1102	52-53	-0.23196361935028875
1102	54-55	-0.3094125325439947
1102	56-57	-0.13627417156705945
1102	58-59	-0.09712870097013138
1102	60-61	-0.21192882693033965
1102	62-63	-0.42298409127244696
1102	64-65	-0.18235770535176954
1102	66-67	-0.15674599990006755
1102	68-69	-0.23054934173248398
1102	70-71	-0.41535848771596307
1102	72-73	-0.024507471898779443
1102	74-75	-0.29305221508501234
1102	76-77	-0.03218285335090343
1102	78-79	-0.4224887386770604
1102	80-81	-0.2699546984120147
1102	82-83	-0.23678485302531982
1102	84-85	-0.222475574765312
1102	86-87	-0.2577390122085248
1102	88-89	-0.2729848713417269
1102	90-91	-0.5511315235861147
1102	92-93	-0.16717123366102982
1102	94-95	-0.12715488162605482
1102	96-97	-0.10051240302361464
1102	98-99	-0.15042467712398633
1102	100	-0.48579487211030425
1103	1	1.769826697928254
1103	2	0.8373643416178744
1103	3	0.4857909608939579
1103	4	1.114727701601593
1103	5	2.868255165727952
1103	6	-0.04548813692024112
1103	7	-0.4441722378862991
1103	8	-0.27396537040657876
1103	9	-0.18774995914035486
1103	10-11	-0.17206847354801624
1103	12-13	-0.44268457830071384
1103	14-15	-0.4700064165452673
1103	16-17	-0.3708854269255113
1103	18-19	-0.2637380895853383
1103	20-21	-0.2557498022735487
1103	22-23	-0.13741690886119784
1103	24-25	-0.18086783121008665
1103	26-27	-0.19526845758898048
1103	28-29	-0.2236445505110538
1103	30-31	-0.17604090229382052
1103	32-33	-0.32426090236386784
1103	34-35	-0.5090160761023625
1103	36-37	-0.3715009197521155
1103	38-39	-0.2722345610435042
1103	40-41	-0.48445384396932667
1103	42-43	-0.1330088557938751
1103	44-45	-0.48669589621709264
1103	46-47	-0.506568531930597
1103	48-49	-0.5894372251119862
1103	50-51	-0.366631708230571
1103	52-53	-0.27534638852390003
1103	54-55	-0.4679669156536974
1103	56-57	-0.5026310073784543
1103	58-59	-0.48948026077518847
1103	60-61	-0.25381624854206564
1103	62-63	-0.3064976218184441
1103	64-65	-0.3010985287271737
1103	66-67	-0.33846527921440384
1103	68-69	-0.3447626617689252
1103	70-71	-0.5611056750252388
1103	72-73	-0.39681971594834664
1103	74-75	-0.5458228923420236
1103	76-77	-0.5464516753818813
1103	78-79	-0.48616235156944754
1103	80-81	-0.3547179636098363
1103	82-83	-0.4205937492653362
1103	84-85	-0.5852789561856326
1103	86-87	-0.4409796210941508
1103	88-89	-0.38521471372490623
1103	90-91	-0.819961570287397
1103	92-93	-0.503424871142343
1103	94-95	-0.49016556631281105
1103	96-97	-0.26466148668789913
1103	98-99	-0.38001719076729756
1103	100	-0.4114879836715275
1104	1	1.769711162736236
1104	2	-0.4236899005042769
1104	3	-0.06832063399784616
1104	4	0.10856678748715431
1104	5	-1.110702574799813
1104	6	-0.33574059781983223
1104	7	-0.15651326696375634
1104	8	-0.23717085029575458
1104	9	-0.10011349007187675
1104	10-11	-0.20041358872008885
1104	12-13	-0.17676149062820912
1104	14-15	-0.3279237819247669
1104	16-17	-0.11781913668350086
1104	18-19	-0.07347549184806468
1104	20-21	-0.035276883722552554
1104	22-23	-0.08963134712909948
1104	24-25	-8.253527711516995E-4
1104	26-27	-0.10776210315341928
1104	28-29	-0.15962588167452196
1104	30-31	-0.14837978296687027
1104	32-33	-0.18751716272076635
1104	34-35	-0.13256899782231812
1104	36-37	-0.3055165709194583
1104	38-39	-0.31564072005954813
1104	40-41	-0.2204951984158896
1104	42-43	-0.24439028257647522
1104	44-45	-0.20737237677307263
1104	46-47	-0.4296194119786634
1104	48-49	-0.3941911406546623
1104	50-51	-0.22268820053812988
1104	52-53	-0.12128694951878316
1104	54-55	-0.2222147595469437
1104	56-57	-0.31124792459143436
1104	58-59	-0.1456992450778003
1104	60-61	-0.16066599997635223
1104	62-63	-0.2533290829315007
1104	64-65	-0.3651532335402621
1104	66-67	-0.6267508910117598
1104	68-69	-0.6224108521577136
1104	70-71	-0.5352166454768437
1104	72-73	-0.2219008106497995
1104	74-75	-0.2539199286992968
1104	76-77	-0.3680260706926859
1104	78-79	-0.24987280277287738
1104	80-81	-0.46111872328123127
1104	82-83	-0.37110755076927404
1104	84-85	-0.1856035275500716
1104	86-87	-0.2823363233456533
1104	88-89	-0.4174944898947288
1104	90-91	-0.3046475868980494
1104	92-93	-0.40674199653874155
1104	94-95	-0.3226453146441486
1104	96-97	-0.2658327246969918
1104	98-99	-0.5317833937527254
1104	100	-0.2237907485937214
1105	1	1.718183259913454
1105	2	0.3941405272378997
1105	3	0.011954245678101216
1105	4	-0.13397304101797225
1105	5	-1.1877376521297833
1105	6	0.2810792523187473
1105	7	-0.2213406085685179
1105	8	-0.25814711608552443
1105	9	-0.1592403844033683
1105	10-11	-0.29560660470927047
1105	12-13	-0.2338734886895466
1105	14-15	-0.3028709821738431
1105	16-17	-0.3981809995357466
1105	18-19	-0.27972712679476075
1105	20-21	-0.13881936184436228
1105	22-23	-0.25332782719920033
1105	24-25	-0.2153191251288007
1105	26-27	-0.10454597242829777
1105	28-29	-0.19977335186031553
1105	30-31	-0.25995195573279517
1105	32-33	-0.22022933667428646
1105	34-35	-0.16649424204123164
1105	36-37	-0.16300197876945077
1105	38-39	-0.1497835024619789
1105	40-41	-0.23366965906402726
1105	42-43	-0.06041605013881224
1105	44-45	-0.16555706035170914
1105	46-47	-0.3775599007214012
1105	48-49	-0.4095987579415308
1105	50-51	-0.209018441314619
1105	52-53	-0.278373630982216
1105	54-55	-0.42003548593671525
1105	56-57	-0.1377477825617106
1105	58-59	-0.1880176837458407
1105	60-61	-0.24839041897848801
1105	62-63	-0.4795916629937409
1105	64-65	-0.5159839870681324
1105	66-67	-0.35333183431382054
1105	68-69	-0.40021698238533077
1105	70-71	-0.6392237293096841
1105	72-73	-0.44221800234498687
1105	74-75	-0.4303224521743161
1105	76-77	-0.5022919054021813
1105	78-79	-0.5416518093351357
1105	80-81	-0.614857074035271
1105	82-83	-0.4389369754766719
1105	84-85	-0.38149601987485227
1105	86-87	-0.49285185915078245
1105	88-89	-0.4697832949031593
1105	90-91	-0.3403261359159586
1105	92-93	-0.3897493836892991
1105	94-95	-0.3175500985583639
1105	96-97	-0.34573868818010567
1105	98-99	-0.4911083272715828
1105	100	-0.37571400760619156
1106	1	1.2675088934500653
1106	2	-0.7088759664370397
1106	3	-0.11526047653619287
1106	4	-0.24471707985945024
1106	5	-1.2060635171676957
1106	6	-0.7086030834976746
1106	7	-0.40518594032891286
1106	8	-0.32270585240554084
1106	9	-0.23193337879342835
1106	10-11	-0.15111134097220003
1106	12-13	-0.08154166804280294
1106	14-15	-0.12578160364384416
1106	16-17	-0.19000821076959085
1106	18-19	-0.08219309134651809
1106	20-21	-0.20613470784366683
1106	22-23	-0.25846525334868886
1106	24-25	-0.22985455275610178
1106	26-27	-0.2577372020173385
1106	28-29	-0.2688035516808185
1106	30-31	-0.2679272548610143
1106	32-33	-0.3168169123482585
1106	34-35	-0.35773611967534436
1106	36-37	-0.4112645297869406
1106	38-39	-0.2861873200135818
1106	40-41	-0.40922018818720574
1106	42-43	-0.2715357236268403
1106	44-45	-0.17517704937917955
1106	46-47	-0.2756351101032237
1106	48-49	-0.33089901830121704
1106	50-51	-0.3019987193064608
1106	52-53	-0.4102370103303983
1106	54-55	-0.28588880607764366
1106	56-57	-0.24265830572294078
1106	58-59	-0.1561164872329499
1106	60-61	-0.2942185593887814
1106	62-63	-0.4588469264771078
1106	64-65	-0.5932784662377699
1106	66-67	-0.5353318945675767
1106	68-69	-0.5617525654039213
1106	70-71	-0.8317923789594275
1106	72-73	-0.5665005068719537
1106	74-75	-0.7874790136506036
1106	76-77	-0.697429268126676
1106	78-79	-0.44249750261811016
1106	80-81	-0.3827762261696819
1106	82-83	-0.5093851207706948
1106	84-85	-0.6819296091281188
1106	86-87	-0.5830489682386357
1106	88-89	-0.6280402010944002
1106	90-91	-0.5880733586931512
1106	92-93	-0.6729143236099588
1106	94-95	-0.6228745492184586
1106	96-97	-0.5667847727954154
1106	98-99	-0.7964680580958188
1106	100	-0.7911407152454117
1107	1	1.2847847248776443
1107	2	-0.47452457692793537
1107	3	-0.13639292307358275
1107	4	-0.22227654164738553
1107	5	-1.135967846049006
1107	6	-0.40395078779678784
1107	7	-0.4234225425382334
1107	8	-0.45716414457909593
1107	9	-0.35543076486541025
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2223	5	-1.1126003180357422
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2223	8	0.2533350039248603
2223	9	0.009385051720798288
2223	10-11	0.1377526771374491
2223	12-13	0.12706942561740675
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2224	9	0.4096079422868826
2224	10-11	0.29862094097082803
2224	12-13	0.2915757723175716
2224	14-15	0.2352431851238066
2224	16-17	0.2588178845540341
2224	18-19	0.042430040348904186
2224	20-21	0.151004981506766
2224	22-23	0.16949142550605245
2224	24-25	0.2612292995012311
2224	26-27	0.1303888428591904
2224	28-29	0.06292945251887261
2224	30-31	0.15944754012341633
2224	32-33	0.11092257473863754
2224	34-35	0.17716622124405745
2224	36-37	0.09368375996152878
2224	38-39	0.12790531732407828
2224	40-41	0.27978614660568724
2224	42-43	0.2633891878135586
2224	44-45	0.2551093428141158
2224	46-47	0.3039560704458921
2224	48-49	0.24693324550620588
2224	50-51	0.269340757960272
2224	52-53	0.2649466622339034
2224	54-55	0.24589325930718076
2224	56-57	0.4659556742201829
2224	58-59	0.3504875680165256
2224	60-61	0.36761999565438686
2224	62-63	0.33022858498278396
2224	64-65	0.24285479962157552
2224	66-67	0.28875683054643986
2224	68-69	0.3531692415999146
2224	70-71	0.3545359833156496
2224	72-73	0.2802876287950937
2224	74-75	0.30847995235027525
2224	76-77	0.4425630024598206
2224	78-79	0.41883000831275297
2224	80-81	0.2975658003780026
2224	82-83	0.4280179150831742
2224	84-85	0.15160210720557643
2224	86-87	0.2075474795573271
2224	88-89	0.49989327368087544
2224	90-91	0.5430261266197718
2224	92-93	0.33074566455833576
2224	94-95	0.5431617267564519
2224	96-97	0.4487431516907492
2224	98-99	0.35331902665608794
2224	100	0.5999157356074747
2225	1	1.182924415536517
2225	2	1.4845699304402302
2225	3	0.8106141406214604
2225	4	1.0037160292940346
2225	5	3.2611975418452808
2225	6	-0.07296795988301241
2225	7	0.37553617138212303
2225	8	0.5673639275193096
2225	9	0.4594577992558939
2225	10-11	0.3168498853862971
2225	12-13	0.37139595628229216
2225	14-15	0.4121320239650146
2225	16-17	0.34860005862512367
2225	18-19	0.2945545642374512
2225	20-21	0.39296580998020403
2225	22-23	0.48279646873143633
2225	24-25	0.46147031857729104
2225	26-27	0.25857819692807027
2225	28-29	0.2693504377732552
2225	30-31	0.1469582081808838
2225	32-33	0.3335734289734589
2225	34-35	0.4264911702744527
2225	36-37	0.3598494230405649
2225	38-39	0.430719313815473
2225	40-41	0.33410848788869885
2225	42-43	0.6090233840742059
2225	44-45	0.5864404398065162
2225	46-47	0.547404500265877
2225	48-49	0.33640782392037494
2225	50-51	0.35198323579442103
2225	52-53	0.3878207554490203
2225	54-55	0.37955868531827264
2225	56-57	0.4175612168686982
2225	58-59	0.5582547040777186
2225	60-61	0.5619641471882133
2225	62-63	0.41416268799670775
2225	64-65	0.36219065377738247
2225	66-67	0.5341839874696177
2225	68-69	0.5873779483152575
2225	70-71	0.4478530379835206
2225	72-73	0.5829457531135276
2225	74-75	0.48173612006794286
2225	76-77	0.580564401160693
2225	78-79	0.5082184520407296
2225	80-81	0.4635129658356263
2225	82-83	0.42500201572641316
2225	84-85	0.41368941142696514
2225	86-87	0.541095359379689
2225	88-89	0.4299706763644835
2225	90-91	0.553843536889552
2225	92-93	0.4002243521502038
2225	94-95	0.6378024107764944
2225	96-97	0.5339635458557126
2225	98-99	0.5846447896197517
2225	100	0.5919779106268379
2226	1	1.282792014559762
2226	2	1.4509266044008413
2226	3	0.9183544444301681
2226	4	0.9965846114143524
2226	5	3.2052125420038458
2226	6	-0.29164680426826095
2226	7	0.19160980852416287
2226	8	0.11705155635259246
2226	9	0.26460954504602086
2226	10-11	0.14952834921783875
2226	12-13	0.20193182346905303
2226	14-15	0.1357854188212002
2226	16-17	0.04900796462835899
2226	18-19	0.15553591710587256
2226	20-21	0.15271472381650852
2226	22-23	0.22885615223761135
2226	24-25	0.17528283245165
2226	26-27	0.1063908059408476
2226	28-29	0.24793239952760615
2226	30-31	0.21888246213705287
2226	32-33	0.17473863685091828
2226	34-35	0.07354227546583303
2226	36-37	0.1521749233101204
2226	38-39	0.12525519685863173
2226	40-41	0.23294819549659707
2226	42-43	0.26213084292683675
2226	44-45	0.20716119266859323
2226	46-47	0.19457690695094243
2226	48-49	0.15960653638034472
2226	50-51	0.11266926313086145
2226	52-53	0.2570573488590995
2226	54-55	0.26853491656807904
2226	56-57	0.3472717582063538
2226	58-59	0.2786650678236313
2226	60-61	0.38133631285592884
2226	62-63	0.38268732884914414
2226	64-65	0.2728084985748964
2226	66-67	0.3057594005289488
2226	68-69	0.38182860318475775
2226	70-71	0.4600957678221036
2226	72-73	0.3042349580734083
2226	74-75	0.34415208112461215
2226	76-77	0.4467993772175234
2226	78-79	0.3160903640059729
2226	80-81	0.32071890881599074
2226	82-83	0.2570929997748763
2226	84-85	0.12539460915633072
2226	86-87	0.2696947631788831
2226	88-89	0.29310493254055103
2226	90-91	0.21594514472896265
2226	92-93	0.11146733569125189
2226	94-95	0.41617110389163514
2226	96-97	0.5493236447996708
2226	98-99	0.3839685139720217
2226	100	0.5641419926995823
2227	1	1.6415806334465533
2227	2	1.643049355811545
2227	3	0.6099268199282637
2227	4	-0.021975790610476054
2227	5	3.0717397232746535
2227	6	0.02942667917437447
2227	7	0.145417392203413
2227	8	0.145794179157555
2227	9	0.004624058346948345
2227	10-11	0.025406326668942825
2227	12-13	-0.02120112081831138
2227	14-15	0.1235935001297861
2227	16-17	0.06614340984944533
2227	18-19	-0.021027792502366083
2227	20-21	0.08572803460200618
2227	22-23	0.22679663811351247
2227	24-25	0.21938416662040794
2227	26-27	0.07580991160396877
2227	28-29	0.07178080586167113
2227	30-31	0.1376399445588632
2227	32-33	0.050772722861935904
2227	34-35	0.09256354405834344
2227	36-37	0.1768537952153295
2227	38-39	0.07084963214790463
2227	40-41	0.16434967208111573
2227	42-43	0.3582737360713253
2227	44-45	0.26431477418790195
2227	46-47	0.21776462243269634
2227	48-49	0.1764500106429452
2227	50-51	0.1982543159227319
2227	52-53	0.28916456400493473
2227	54-55	0.18597866287515075
2227	56-57	0.3662232308215536
2227	58-59	0.3941250632735702
2227	60-61	0.25825110688586506
2227	62-63	0.22637923768387225
2227	64-65	0.31513909281571983
2227	66-67	0.3437878186803829
2227	68-69	0.2977797278856613
2227	70-71	0.43256471793609563
2227	72-73	0.3411238636564988
2227	74-75	0.3263181433368061
2227	76-77	0.48146040586103567
2227	78-79	0.4623833984207124
2227	80-81	0.37117247610969883
2227	82-83	0.18244979840921616
2227	84-85	0.22831290683777183
2227	86-87	0.2583064306154341
2227	88-89	0.32705108521045645
2227	90-91	0.35074755018604975
2227	92-93	0.38072556429065685
2227	94-95	0.28682264134783253
2227	96-97	0.3254695642124972
2227	98-99	0.4154905884150253
2227	100	0.4213916369265789
2228	1	1.4357164947965604
2228	2	1.4893979967920892
2228	3	-0.11286393599587896
2228	4	-0.12791573473677254
2228	5	3.214287583275709
2228	6	0.2507531980632578
2228	7	0.41992648076792705
2228	8	0.5444334748379163
2228	9	0.39258292757440927
2228	10-11	0.31483048099477884
2228	12-13	0.40918918053830566
2228	14-15	0.35710726836242657
2228	16-17	0.3385933860623922
2228	18-19	0.2700696808164693
2228	20-21	0.31953320417994746
2228	22-23	0.2809836900499505
2228	24-25	0.4472821075611151
2228	26-27	0.2893308146257567
2228	28-29	0.31820425732188795
2228	30-31	0.2756262093600981
2228	32-33	0.34624294102091824
2228	34-35	0.28695649926995515
2228	36-37	0.32491572752000053
2228	38-39	0.3366688181769888
2228	40-41	0.32258226090048225
2228	42-43	0.5210636747715824
2228	44-45	0.38956660783401276
2228	46-47	0.5277255071253251
2228	48-49	0.4064925009788709
2228	50-51	0.33691094311765823
2228	52-53	0.39517893387887426
2228	54-55	0.3903955457656352
2228	56-57	0.6651267843392361
2228	58-59	0.5740769691166463
2228	60-61	0.49694476122191844
2228	62-63	0.5516563707775504
2228	64-65	0.3594037730622759
2228	66-67	0.4634655485597534
2228	68-69	0.534378914323355
2228	70-71	0.6176675223141856
2228	72-73	0.5401121776201236
2228	74-75	0.49536190116577217
2228	76-77	0.6511745260837003
2228	78-79	0.5860182962159826
2228	80-81	0.3009168256565289
2228	82-83	0.4816302263214496
2228	84-85	0.41615075794222633
2228	86-87	0.4552644871296465
2228	88-89	0.4227462124068424
2228	90-91	0.38439966274962245
2228	92-93	0.4662149447580006
2228	94-95	0.7646742817788734
2228	96-97	0.5575907351669755
2228	98-99	0.5575172028501143
2228	100	0.7482461713384723
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	28.0
13	46.0
14	137.0
15	520.0
16	1912.0
17	5120.0
18	8599.0
19	10753.0
20	12634.0
21	14629.0
22	16886.0
23	19307.0
24	21378.0
25	24520.0
26	28115.0
27	31265.0
28	35383.0
29	40421.0
30	46355.0
31	53301.0
32	62928.0
33	75562.0
34	91892.0
35	119129.0
36	167989.0
37	274891.0
38	672330.0
39	1242639.0
40	1437.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.95588238524975	18.61463381054244	19.482808242201262	34.94667556200655
2	20.089853041542018	50.74794199066339	12.480397205380143	16.681807762414454
3	39.44564263514222	15.803119826681348	24.46889020413901	20.28234733403742
4	17.824056112336358	16.056065584734554	22.467563626848026	43.65231467608106
5	17.9841804197062	35.32091579935373	26.15561732108657	20.539286459853507
6	17.473548808531653	27.322849498410285	31.74668931955935	23.45691237349871
7	20.82976338159681	26.813419144205053	35.98303565428084	16.373781819917298
8	20.76343451704762	27.634202318296087	34.29799679037124	17.30436637428505
9	20.87235930440079	27.971333463415395	27.8979269226686	23.25838030951522
10-11	19.485686698546512	30.22620642724425	28.84201750134005	21.44608937286919
12-13	24.17907884368952	26.60285827732608	27.34565714762507	21.872405731359333
14-15	21.59236675868728	27.324359186223074	28.67624403957395	22.407030015515694
16-17	20.988491633569872	29.571635011381098	28.238320941447814	21.201552413601217
18-19	21.600856723483957	29.35571718774705	29.682377917390536	19.36104817137846
20-21	22.8444498843709	28.807245982038935	29.89368551157476	18.454618622015403
22-23	21.9412182758586	30.33630000516216	28.601620008655544	19.1208617103237
24-25	22.151048648634607	28.180758006134205	26.745613058247653	22.92258028698354
26-27	20.16227033671233	26.634415622574153	29.92548635485715	23.277827685856366
28-29	20.0309567871173	27.863121327229635	30.65493003473913	21.45099185091393
30-31	20.310853944882577	29.88434500673987	28.22019824946795	21.584602798909607
32-33	20.785316873731986	30.00634393545419	28.801759159665558	20.40658003114827
34-35	21.3766275002784	29.208108679341333	29.945859023728723	19.469404796651546
36-37	21.949107612170614	28.02670491642011	28.75583543039252	21.268352041016758
38-39	21.514670713312178	29.245195420121934	28.885448504293066	20.35468536227282
40-41	20.90319669097355	29.577654331409132	30.016655519697665	19.502493457919652
42-43	20.68312325918971	29.909145895445704	29.17167055777031	20.236060287594277
44-45	20.66166857190351	29.95337499638811	28.996314089088465	20.388642342619914
46-47	19.75708507282107	28.063867155381754	28.934729782751866	23.244317989045307
48-49	20.92016608513925	28.13866531260115	30.123758687604035	20.817409914655563
50-51	21.97590862914827	30.425014455666638	28.20499417715034	19.39408273803475
52-53	20.36828077037897	30.279716181636868	28.30969506035337	21.042307987630796
54-55	22.370692209587688	28.306401398268726	28.112994790588473	21.209911601555113
56-57	21.455793140777928	28.55743850854797	29.428357031785506	20.558411318888595
58-59	21.07421875	27.954608920407313	29.10315357439734	21.868018755195344
60-61	19.746748441081206	30.29971921361631	29.424098150075995	20.529434195226486
62-63	20.494212593727244	31.051977475426153	27.515648604309284	20.938161326537323
64-65	19.74987845372029	30.275989191918125	28.86473745597752	21.109394898384064
66-67	20.215758738251626	30.051391071803675	29.605773435792976	20.127076754151723
68-69	20.802085771695594	30.17351345261707	29.15773705264135	19.866663723045985
70-71	19.779751157995484	30.713332361505625	29.29362518899506	20.213291291503836
72-73	20.226294086536605	30.60940740045719	28.50032482637779	20.66397368662842
74-75	20.05190079435552	29.203043920227444	30.46363324391003	20.281422041507
76-77	19.56807020015863	30.410047443157005	28.71807700187039	21.303805354813974
78-79	19.634058167459767	29.33971125029098	28.464319583702775	22.561910998546477
80-81	20.725109103529686	29.476913426893002	28.34183738071012	21.456140088867187
82-83	20.36490940087471	27.982745404623934	30.028697055004905	21.623648139496453
84-85	20.791355624983158	28.781743621244328	28.425148866581583	22.00175188719093
86-87	21.46574778084008	29.004998852312596	28.833413904127358	20.695839462719963
88-89	20.70252754206266	29.424107668986828	28.307799047240888	21.565565741709623
90-91	20.414891430719777	29.712880104489876	27.381142927826858	22.49108553696349
92-93	20.265270005230338	29.942109154000168	28.455472488455758	21.337148352313733
94-95	20.309667813488296	29.459285018345145	27.944337648010283	22.286709520156283
96-97	20.19571073342582	29.985451804109402	28.164752068678133	21.654085393786644
98-99	20.984287234177255	30.39342464401399	27.544108045597117	21.078180076211638
100	20.796680115333654	31.40085717801362	26.03614095224614	21.766321754406583
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	9.0
2	16.5
3	23.5
4	34.0
5	39.5
6	43.0
7	41.5
8	50.0
9	63.5
10	68.0
11	85.5
12	106.0
13	121.0
14	141.0
15	190.5
16	272.0
17	368.5
18	530.0
19	820.5
20	1303.5
21	2036.0
22	3222.0
23	5102.0
24	7782.5
25	11404.5
26	16340.5
27	21923.5
28	28367.5
29	36165.5
30	44938.5
31	54576.5
32	65844.0
33	78350.5
34	91200.5
35	105375.0
36	119870.0
37	131249.5
38	139497.5
39	145341.5
40	148248.5
41	148244.0
42	146013.0
43	143447.0
44	142224.5
45	139018.0
46	129814.5
47	118923.0
48	110667.5
49	106582.5
50	103721.0
51	96151.5
52	83933.5
53	71006.0
54	59141.5
55	48667.0
56	39156.0
57	29817.5
58	21083.0
59	14604.5
60	11192.5
61	9397.0
62	7707.0
63	5736.5
64	4257.5
65	3853.5
66	4305.5
67	4117.5
68	2890.5
69	2527.5
70	3160.0
71	3053.0
72	1862.5
73	833.5
74	408.5
75	232.5
76	143.0
77	106.0
78	96.5
79	85.5
80	63.0
81	50.0
82	42.5
83	37.0
84	34.0
85	23.0
86	16.5
87	21.0
88	23.5
89	16.0
90	13.5
91	15.5
92	16.5
93	19.5
94	17.0
95	18.0
96	19.0
97	22.5
98	45.5
99	105.0
100	108.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.333664707102708
2	0.0061361504649026805
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	2.272648320334326E-4
30-31	0.009756154575149499
32-33	0.0
34-35	0.0
36-37	0.0
38-39	8.116601144051165E-4
40-41	0.001525921015081619
42-43	1.2986561830481864E-4
44-45	0.0
46-47	0.0018668182631317676
48-49	0.0
50-51	0.0
52-53	0.0012499565761838792
54-55	0.018976613474791622
56-57	0.012012569693195724
58-59	0.013863154754039389
60-61	2.434980343215349E-4
62-63	0.011671672445145574
64-65	1.4609882059292095E-4
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.6233202288102328E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3080107.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.24960917073047
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.86793449677805	62.89792642186875
2	8.719034133595564	12.773316852642672
3	2.4362831691726496	5.3537036991337565
4	1.0240175332945496	3.0003553639120493
5	0.5652750039327697	2.0703086556029255
6	0.32919980173353514	1.4468254089638044
7	0.21978153613827991	1.1269238137550246
8	0.16166068580048312	0.9473265642526115
9	0.11001827095180614	0.7252915813973395
>10	0.5277474448025316	6.544537667521119
>50	0.027173057309743922	1.344024373059369
>100	0.011198273058785985	1.4342674156106536
>500	6.314872026325244E-4	0.29746034675954974
>1k	4.510622862915065E-5	0.03773183552042526
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	1.623320228810233E-5	0.0	0.0	0.0	0.0
32-33	3.246640457620466E-5	0.0	0.0	0.0	0.0
34-35	3.246640457620466E-5	0.0	0.0	0.0	0.0
36-37	3.246640457620466E-5	0.0	0.0	0.0	0.0
38-39	3.246640457620466E-5	0.0	0.0	0.0	0.0
40-41	3.246640457620466E-5	0.0	0.0	0.0	0.0
42-43	3.246640457620466E-5	0.0	0.0	0.0	0.0
44-45	3.246640457620466E-5	0.0	0.0	0.0	0.0
46-47	3.246640457620466E-5	0.0	0.0	0.0	0.0
48-49	3.246640457620466E-5	0.0	0.0	0.0	0.0
50-51	3.246640457620466E-5	0.0	0.0	0.0	0.0
52-53	3.246640457620466E-5	0.0	0.0	0.0	0.0
54-55	1.4609882059292095E-4	0.0	0.0	0.0	0.0
56-57	1.9479842745722794E-4	0.0	0.0	0.0	0.0
58-59	2.1103162974533027E-4	0.0	0.0	0.0	0.0
60-61	2.4349803432153496E-4	0.0	1.623320228810233E-5	0.0	0.0
62-63	2.597312366096373E-4	0.0	3.246640457620466E-5	0.0	0.0
64-65	3.571304503382512E-4	0.0	3.246640457620466E-5	0.0	0.0
66-67	4.707628663549676E-4	0.0	3.246640457620466E-5	1.623320228810233E-5	0.0
68-69	5.681620800835815E-4	0.0	3.246640457620466E-5	1.785652251691256E-4	0.0
70-71	5.843952823716838E-4	0.0	3.246640457620466E-5	3.2466404576204656E-4	0.0
72-73	6.168616869478885E-4	0.0	3.246640457620466E-5	3.2466404576204656E-4	0.0
74-75	6.493280915240931E-4	0.0	3.246640457620466E-5	3.2466404576204656E-4	0.0
76-77	7.629605075408094E-4	0.0	3.246640457620466E-5	3.2466404576204656E-4	0.0
78-79	9.090593281337304E-4	0.0	3.246640457620466E-5	3.2466404576204656E-4	0.0
80-81	0.0012986561830481862	0.0	3.246640457620466E-5	3.2466404576204656E-4	0.0
82-83	0.0014285218013530049	0.0	3.246640457620466E-5	3.2466404576204656E-4	0.0
84-85	0.0015746206219459258	0.0	3.246640457620466E-5	3.2466404576204656E-4	0.0
86-87	0.0016557866333864375	0.0	3.246640457620466E-5	3.2466404576204656E-4	0.0
88	0.0016557866333864375	0.0	3.246640457620466E-5	3.2466404576204656E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	12635	0.0	78.27358	8
CACCGGC	12520	0.0	76.48068	9
CTTCACC	13715	0.0	74.71088	6
CCTTCAC	14950	0.0	71.490395	5
GTGTGGC	4700	0.0	71.30603	7
CGTAGTG	2665	0.0	70.97964	4
CGGGGCA	1450	0.0	70.56908	94
TGCAGCC	90	0.0	67.79954	3
TTCACCG	15205	0.0	66.61789	7
GCGTAGT	1905	0.0	64.80174	3
TGTGGCA	5335	0.0	62.3789	8
GGCGCGC	350	0.0	61.690132	6
CAGCGTA	1275	0.0	61.45431	1
GCCTTCA	4365	0.0	60.648598	4
GCAGCCT	70	4.947651E-10	60.50521	1
ACCTTCA	5910	0.0	60.36039	4
ATCGGAA	4405	0.0	57.966743	94
TGTGTGG	5395	0.0	57.857025	6
CGGGCCC	760	0.0	57.43746	7
GCAGCCC	115	0.0	57.14209	4
>>END_MODULE