FastQCFastQC Report
Tue 7 Mar 2017
1SN_30A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_30A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3840203
Sequences flagged as poor quality0
Sequence length93
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA172620.4495074869739959No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG104220.2713919029801289No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT78400.2041558740514499No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA78390.2041298337613923No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA71910.1872557258040786No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG67290.17522511179747527No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG57700.1502524736322533No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG57120.1487421368089135No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA48280.1257225203980102No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA48000.12499339227639789No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT46890.12210292008000619No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA45430.11830103773159908No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC45140.11754586931992919No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT42540.11077539390495764No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC42150.1097598225927119No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCCG257.804301E-786.999733
GCAGCAT500.086.999733
GCTGCCA700.086.9997253
GCTGCAT750.086.9997253
AGCTGCA1650.086.9997252
CAGCTGC4850.084.309011
AGCAGCA1500.084.099742
AGCAGCG1100.083.04522
CTGCAGC2150.082.9532241
CAGCAGC3800.082.420791
GCTGCAA850.081.8820953
GCAGCAA850.081.8820953
GCTGCCC800.081.562243
CAGCCCT195200.081.1165541
AGCTGCC1400.080.785452
GCAGCTA600.079.749753
CAGCGTA79000.078.905451
AGCCCTT167500.078.5594562
TGCAGCA501.8189894E-1278.299762
CTGCTGC3000.078.299751
Produced by FastQC (version 0.11.5)