FastQCFastQC Report
Tue 7 Mar 2017
1SN_30A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_30A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3840203
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTGCG404.0563464E-1092.59361
TCACCGG137300.080.202298
CACCGGC133900.078.698259
CTTCACC161150.070.429616
CCTTCAC175350.068.4469765
CGGGGCA18200.067.8287394
CGTAGTG31000.066.773824
TTCACCG171550.065.749497
GTGTGGC59800.065.384257
GGCCCGA9300.062.5846829
CAGCTGC1200.061.729061
AACGGGC9700.060.9716765
ACCTTCA70950.058.879884
CGGGCCC10350.058.049567
ATGCAGC900.057.372282
GCGTAGT20950.056.9087453
GGCGCGC4400.056.539576
AGGCTGC504.6063178E-756.329152
TGTGGCA69450.056.299188
TGTGTGG66400.055.562766
Produced by FastQC (version 0.11.5)