##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1SN_30A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3840203
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.793955684113575	12.0	12.0	12.0	2.0	27.0
2	14.558199397271446	12.0	12.0	12.0	12.0	22.0
3	13.676561108878879	12.0	12.0	12.0	12.0	22.0
4	13.766389693461518	12.0	12.0	12.0	12.0	22.0
5	22.430425162419798	22.0	22.0	22.0	22.0	27.0
6	26.799383261770277	27.0	22.0	32.0	22.0	32.0
7	34.95557135911825	37.0	37.0	37.0	27.0	41.0
8	37.58548154876188	41.0	37.0	41.0	27.0	41.0
9	38.54219138936145	41.0	41.0	41.0	32.0	41.0
10-11	38.89480595166454	41.0	41.0	41.0	37.0	41.0
12-13	39.0722463109372	41.0	41.0	41.0	37.0	41.0
14-15	39.02519359002636	41.0	41.0	41.0	37.0	41.0
16-17	38.93787021154871	41.0	41.0	41.0	37.0	41.0
18-19	38.872306490047535	41.0	41.0	41.0	37.0	41.0
20-21	38.885721666276496	41.0	41.0	41.0	37.0	41.0
22-23	38.86782769035908	41.0	41.0	41.0	37.0	41.0
24-25	38.892074715841844	41.0	41.0	41.0	37.0	41.0
26-27	38.862985368221416	41.0	41.0	41.0	37.0	41.0
28-29	38.805087647710295	41.0	41.0	41.0	34.5	41.0
30-31	38.68656018965664	41.0	41.0	41.0	32.0	41.0
32-33	38.73271738499241	41.0	41.0	41.0	32.0	41.0
34-35	38.71248590243797	41.0	41.0	41.0	32.0	41.0
36-37	38.62545690943942	41.0	41.0	41.0	32.0	41.0
38-39	38.52735037705038	41.0	41.0	41.0	32.0	41.0
40-41	38.583717970117725	41.0	41.0	41.0	32.0	41.0
42-43	38.32823473134103	41.0	41.0	41.0	32.0	41.0
44-45	38.44648264688091	41.0	41.0	41.0	32.0	41.0
46-47	38.459934930523204	41.0	41.0	41.0	32.0	41.0
48-49	38.40519094954095	41.0	41.0	41.0	32.0	41.0
50-51	38.29689029460161	41.0	41.0	41.0	32.0	41.0
52-53	38.25197053384938	41.0	41.0	41.0	32.0	41.0
54-55	38.12523478576523	41.0	39.0	41.0	32.0	41.0
56-57	37.919383298226684	41.0	37.0	41.0	29.5	41.0
58-59	37.90796541224513	41.0	37.0	41.0	29.5	41.0
60-61	37.9005115094176	41.0	37.0	41.0	29.5	41.0
62-63	37.88469112179747	41.0	37.0	41.0	29.5	41.0
64-65	37.86633037368077	41.0	37.0	41.0	29.5	41.0
66-67	37.752799917087714	41.0	37.0	41.0	27.0	41.0
68-69	37.76591667159262	41.0	37.0	41.0	27.0	41.0
70-71	37.751003527678094	41.0	37.0	41.0	27.0	41.0
72-73	37.59206414348408	41.0	37.0	41.0	27.0	41.0
74-75	37.5076208210868	41.0	37.0	41.0	27.0	41.0
76-77	36.46968975338022	39.0	34.5	41.0	24.5	41.0
78-79	36.98529556901029	41.0	37.0	41.0	27.0	41.0
80-81	37.63566105750139	41.0	37.0	41.0	27.0	41.0
82-83	37.82212828332253	41.0	37.0	41.0	27.0	41.0
84-85	37.73353856553938	41.0	37.0	41.0	27.0	41.0
86-87	37.592243691284025	41.0	37.0	41.0	27.0	41.0
88-89	37.5017393611744	41.0	37.0	41.0	27.0	41.0
90-91	37.341385468424455	41.0	37.0	41.0	27.0	41.0
92-93	37.23222756713643	41.0	37.0	41.0	27.0	41.0
94-95	37.07529120205364	41.0	37.0	41.0	27.0	41.0
96-97	37.03187943449865	41.0	37.0	41.0	27.0	41.0
98-99	36.907539783704145	41.0	37.0	41.0	27.0	41.0
100	36.77544312110584	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.3648120966735373
1101	2	-0.5261030763979733
1101	3	0.002230445235200662
1101	4	0.1812873099208563
1101	5	-1.1591737380153404
1101	6	-0.7293671443182994
1101	7	-0.41758634886348744
1101	8	-0.13320101724847433
1101	9	-0.07402317087618115
1101	10-11	-0.12223266258115473
1101	12-13	-0.1273160048372688
1101	14-15	-0.04306125611030609
1101	16-17	-0.11524221757826325
1101	18-19	-0.030253326367102318
1101	20-21	0.03482732560621571
1101	22-23	0.07589528187636319
1101	24-25	0.0021107135085500772
1101	26-27	-0.03635620857922106
1101	28-29	0.06283555152430154
1101	30-31	0.012138097973135586
1101	32-33	-0.15217844453790264
1101	34-35	-0.0377417176019037
1101	36-37	-0.07727219898556115
1101	38-39	-0.0017813005910554125
1101	40-41	-0.023081069879353322
1101	42-43	0.014691209672633931
1101	44-45	-0.1313437281033245
1101	46-47	-0.04955385993836359
1101	48-49	-0.020670102564189108
1101	50-51	-0.09767000318763053
1101	52-53	-0.0065907146836323705
1101	54-55	-0.05346359144244417
1101	56-57	-0.009753873222692278
1101	58-59	-0.08938937256129265
1101	60-61	0.03216435672594997
1101	62-63	-0.21703551222222472
1101	64-65	-0.09428038777072345
1101	66-67	-0.1728418836955683
1101	68-69	-0.016038977543104238
1101	70-71	-0.12333144536739127
1101	72-73	0.06799643206004902
1101	74-75	0.0042518147549657215
1101	76-77	-0.47922785021083314
1101	78-79	-0.23001108086932476
1101	80-81	-0.16774605588694413
1101	82-83	-0.14113672979531344
1101	84-85	-0.11516299738634217
1101	86-87	-0.13449461073221158
1101	88-89	-0.4112418963860165
1101	90-91	-0.2353363680036935
1101	92-93	-0.08755094201534064
1101	94-95	0.04970461151530259
1101	96-97	-0.21223087575486943
1101	98-99	-0.39782009242731675
1101	100	-0.5286423199401469
1102	1	1.766190839432813
1102	2	0.7021984395697878
1102	3	-0.1178967072407282
1102	4	-0.02693408208815562
1102	5	-0.7463598709200383
1102	6	-0.29651877794509574
1102	7	-0.03822730543756592
1102	8	-0.09571665686262776
1102	9	-0.19996659276233686
1102	10-11	-0.08979895436502972
1102	12-13	-0.11863871697887163
1102	14-15	-0.05604191651001145
1102	16-17	-0.14585743130357542
1102	18-19	-0.06808382014898484
1102	20-21	0.019097514966190943
1102	22-23	0.02240902982106263
1102	24-25	0.018463564261445242
1102	26-27	-0.003187943001471183
1102	28-29	-0.09400648354703378
1102	30-31	-0.08832973048507853
1102	32-33	-0.03807646986661695
1102	34-35	-0.03205231990033042
1102	36-37	-0.03263178288847257
1102	38-39	-0.07719688001572678
1102	40-41	-0.04038528296767652
1102	42-43	0.038602841685019484
1102	44-45	0.028825925517601547
1102	46-47	-0.3111620071025314
1102	48-49	-0.2501766365449214
1102	50-51	0.027400407978475982
1102	52-53	-0.04726932916735649
1102	54-55	-0.12341789656978364
1102	56-57	-0.07299880581221885
1102	58-59	-0.04377188637592866
1102	60-61	-0.02921698313054577
1102	62-63	-0.09328891871882661
1102	64-65	-0.2686974008632532
1102	66-67	-0.1488450563656798
1102	68-69	-0.35996326163044756
1102	70-71	-0.30802243466840906
1102	72-73	-0.0768234996082171
1102	74-75	-0.35209694732460406
1102	76-77	-0.12290646293977403
1102	78-79	-0.19111604044760355
1102	80-81	-0.07540073671598435
1102	82-83	-0.3050403248446898
1102	84-85	-0.28433431279853494
1102	86-87	0.051713399206946065
1102	88-89	-0.21820343302526624
1102	90-91	-0.26183720065412075
1102	92-93	-0.20038656857381199
1102	94-95	-0.12792209787478726
1102	96-97	-0.12680813676489322
1102	98-99	-0.1569867927275581
1102	100	-0.4191799366121103
1103	1	1.4341039528564679
1103	2	0.6778486403028996
1103	3	0.41041736855019195
1103	4	1.099436904374281
1103	5	2.9306622610077753
1103	6	-0.1235778893929087
1103	7	-0.5615888247829872
1103	8	-0.27836291491309595
1103	9	-0.24139895083206397
1103	10-11	-0.2750188430485707
1103	12-13	-0.38999119568641305
1103	14-15	-0.3358792606066956
1103	16-17	-0.31512484091968673
1103	18-19	-0.4278947297372113
1103	20-21	-0.290086632246485
1103	22-23	-0.15659128149071222
1103	24-25	-0.25675412904427475
1103	26-27	-0.2625722834793791
1103	28-29	-0.27931229787925105
1103	30-31	-0.12826095397633708
1103	32-33	-0.4136503722226905
1103	34-35	-0.3716674629687873
1103	36-37	-0.32391640282718015
1103	38-39	-0.15370777682756653
1103	40-41	-0.36850447398893493
1103	42-43	-0.12946421811024322
1103	44-45	-0.4103303475946021
1103	46-47	-0.49947386952072037
1103	48-49	-0.40273776072232437
1103	50-51	-0.2858413193160061
1103	52-53	-0.2407048795323874
1103	54-55	-0.30444056474561876
1103	56-57	-0.2688345159719603
1103	58-59	-0.4477530781061887
1103	60-61	-0.2431497145944448
1103	62-63	-0.23532473576827329
1103	64-65	-0.42053791926034023
1103	66-67	-0.4775462024608714
1103	68-69	-0.37312626123635795
1103	70-71	-0.38511447678367716
1103	72-73	-0.2929199378776133
1103	74-75	-0.41032286609922863
1103	76-77	-0.5687907593301702
1103	78-79	-0.6211469196964288
1103	80-81	-0.2917830239757748
1103	82-83	-0.352017811424993
1103	84-85	-0.4327043347343178
1103	86-87	-0.2865119077410654
1103	88-89	-0.5166762958159481
1103	90-91	-0.6057592431472685
1103	92-93	-0.2645650204005676
1103	94-95	-0.3812274911263387
1103	96-97	-0.19309127212433452
1103	98-99	-0.27099852180354134
1103	100	-0.3518025313687545
1104	1	1.6421044829409226
1104	2	-0.5057827695056787
1104	3	0.058208299208519776
1104	4	0.22498571463717632
1104	5	-1.1429287298612323
1104	6	-0.39331741338283166
1104	7	-0.36131755262351106
1104	8	-0.04287757794214997
1104	9	-0.008554313111652334
1104	10-11	-0.11990134939735242
1104	12-13	-0.03583257151484531
1104	14-15	-0.035277892038109826
1104	16-17	0.009301879225965592
1104	18-19	-0.023667882400090434
1104	20-21	-0.030249190278496485
1104	22-23	0.022340220218382
1104	24-25	-0.03370362985740627
1104	26-27	-0.1699944021174673
1104	28-29	0.010187906664206992
1104	30-31	-0.12464120098417197
1104	32-33	-0.01821340185700393
1104	34-35	-0.0340197811519829
1104	36-37	-0.14036268712241906
1104	38-39	-0.1805497842919621
1104	40-41	-0.02789136217712951
1104	42-43	0.016402185482810694
1104	44-45	-0.1368661880737463
1104	46-47	-0.3238430618189412
1104	48-49	-0.23746835754268858
1104	50-51	-0.1866243282366895
1104	52-53	-0.22841417646466766
1104	54-55	-0.14969884767531738
1104	56-57	-0.3204203399579981
1104	58-59	-0.1886017389947412
1104	60-61	-0.12638600982268144
1104	62-63	-0.1738752716865406
1104	64-65	-0.25557776202116145
1104	66-67	-0.6051417076904215
1104	68-69	-0.4652756096502131
1104	70-71	-0.2981068035231189
1104	72-73	-0.05142667700835801
1104	74-75	-0.18811097708277913
1104	76-77	-0.489984900600561
1104	78-79	-0.26947396667948453
1104	80-81	-0.21045557294736028
1104	82-83	-0.2928581177216145
1104	84-85	-0.23611763567679844
1104	86-87	-0.42593132121989896
1104	88-89	-0.44576894752021445
1104	90-91	-0.42568942828791734
1104	92-93	-0.3915786751168042
1104	94-95	-0.42175293373965417
1104	96-97	-0.2386499962629074
1104	98-99	-0.1976638404520017
1104	100	-0.07455395777127904
1105	1	1.3813717239028271
1105	2	0.3458742663949206
1105	3	0.029193224304354004
1105	4	-0.2179381624228789
1105	5	-1.0315650714889664
1105	6	0.42321614719692136
1105	7	0.3113284548164117
1105	8	0.05868504094374316
1105	9	0.24597113244673352
1105	10-11	0.021533522921799886
1105	12-13	0.026086054852470397
1105	14-15	0.10386970216102043
1105	16-17	0.05785548318913669
1105	18-19	0.06835412195208335
1105	20-21	0.12200680572071576
1105	22-23	0.10421334657669945
1105	24-25	0.08001613075915515
1105	26-27	-0.03762797279603802
1105	28-29	0.004958397231497713
1105	30-31	-0.0034418316759996515
1105	32-33	0.00654728732495613
1105	34-35	-0.03946492797908263
1105	36-37	-0.1638764145954852
1105	38-39	0.05820283206229959
1105	40-41	0.0016808768687681663
1105	42-43	0.09384393744758768
1105	44-45	0.12452576081793865
1105	46-47	-0.187919912427013
1105	48-49	-0.062683182189879
1105	50-51	-0.03674345622466291
1105	52-53	-0.12267204835357859
1105	54-55	-0.06431603682890596
1105	56-57	0.07787576148392361
1105	58-59	-0.019548765861742368
1105	60-61	-0.1526913509897483
1105	62-63	-0.34146166844274006
1105	64-65	-0.3104802185236224
1105	66-67	-0.13404368968686242
1105	68-69	-0.21198899832874218
1105	70-71	-0.3306395174623944
1105	72-73	-0.22247520389542075
1105	74-75	-0.09066768954458126
1105	76-77	-0.2923165685514846
1105	78-79	-0.21685749136002386
1105	80-81	-0.36917766459205126
1105	82-83	-0.20281247245567613
1105	84-85	-0.07003629109551213
1105	86-87	-0.26609749516578773
1105	88-89	-0.12833712836373223
1105	90-91	-0.05309005870305583
1105	92-93	-0.1322676184347813
1105	94-95	-0.02733614383515004
1105	96-97	-0.15771429597917574
1105	98-99	-0.2593427928589094
1105	100	-0.2937895742210088
1106	1	1.3982747399521234
1106	2	-0.6201488195506055
1106	3	-0.19248089519277656
1106	4	-0.19968247443954823
1106	5	-1.1771372909062237
1106	6	-0.6388441482944991
1106	7	-0.2686550667885257
1106	8	-0.2055336038968889
1106	9	-0.06708314651840652
1106	10-11	-0.0572296655218949
1106	12-13	-0.02248404042627783
1106	14-15	-0.06894720764207563
1106	16-17	-0.1108592261125807
1106	18-19	-0.008563214074023051
1106	20-21	0.0472248051029851
1106	22-23	-0.040508855692067414
1106	24-25	-0.16710428434618763
1106	26-27	-0.0887623740382395
1106	28-29	-0.11170742536098999
1106	30-31	0.0027331738406246586
1106	32-33	-0.15821650885214922
1106	34-35	-0.11858137869195673
1106	36-37	-0.26374468734582734
1106	38-39	-0.12836245268267277
1106	40-41	-0.1499497280206299
1106	42-43	0.02662264514245294
1106	44-45	-0.03763940658350151
1106	46-47	-0.2190579380203772
1106	48-49	-0.2196111707766235
1106	50-51	-0.20024105500588973
1106	52-53	-0.23648046453106275
1106	54-55	-0.14188038155086957
1106	56-57	-0.21046213071127085
1106	58-59	-0.21248582014966644
1106	60-61	-0.14768517288354843
1106	62-63	-0.308674425263078
1106	64-65	-0.4865406986777998
1106	66-67	-0.33357171441375044
1106	68-69	-0.30685591625390174
1106	70-71	-0.5264273892189308
1106	72-73	-0.2645492440151287
1106	74-75	-0.42564800108374357
1106	76-77	-0.2713060550047004
1106	78-79	-0.22401984833897615
1106	80-81	-0.3571943521107883
1106	82-83	-0.4059463303897246
1106	84-85	-0.41932861241490826
1106	86-87	-0.30191250993622276
1106	88-89	-0.47761674550546473
1106	90-91	-0.45639541872911593
1106	92-93	-0.4178835867334243
1106	94-95	-0.3247063618319501
1106	96-97	-0.32955625704255453
1106	98-99	-0.6931442514535391
1106	100	-0.7007095527160629
1107	1	1.7180983342953287
1107	2	-0.44031516987710084
1107	3	-0.21167046674996293
1107	4	-0.1976462917062225
1107	5	-1.2047820874460093
1107	6	-0.23505447531791646
1107	7	-0.44151312337292836
1107	8	-0.301469336392195
1107	9	-0.276459287324208
1107	10-11	-0.20249017089534505
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2224	26-27	0.2741055961007248
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2224	30-31	0.12120794024936288
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2224	34-35	0.27393950231153497
2224	36-37	0.048501772240534535
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2224	40-41	0.16768822808757733
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2224	52-53	0.2945081069179807
2224	54-55	0.3345116128143033
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2224	60-61	0.3574632047394104
2224	62-63	0.31075382974398735
2224	64-65	0.3778769517713272
2224	66-67	0.3027538587418235
2224	68-69	0.31782537953142764
2224	70-71	0.5135296156859468
2224	72-73	0.4277200870093836
2224	74-75	0.35458859994167113
2224	76-77	0.5462708440406274
2224	78-79	0.3650454674433732
2224	80-81	0.2952008341179422
2224	82-83	0.3258239762677775
2224	84-85	0.10851020820327761
2224	86-87	0.3637318882681271
2224	88-89	0.31487542886497266
2224	90-91	0.5439927266943911
2224	92-93	0.37242956303789043
2224	94-95	0.5151443329556074
2224	96-97	0.46993230578767964
2224	98-99	0.4787645758019963
2224	100	0.6288251864913406
2225	1	1.4461736777589351
2225	2	1.5861203236479628
2225	3	1.016049204912628
2225	4	0.9932650868434987
2225	5	3.2712507599663
2225	6	0.1805770546774994
2225	7	0.2021539746570582
2225	8	0.3599537524112577
2225	9	0.4197352166599586
2225	10-11	0.3061650649994334
2225	12-13	0.2195882188830538
2225	14-15	0.34559298836045826
2225	16-17	0.3636459538409298
2225	18-19	0.218494226935654
2225	20-21	0.24135941838804342
2225	22-23	0.21050500184978205
2225	24-25	0.2649399660939906
2225	26-27	0.2920173087131843
2225	28-29	0.20956482442015556
2225	30-31	0.27152303586072435
2225	32-33	0.33867690856734356
2225	34-35	0.31587667648809514
2225	36-37	0.23243213179151923
2225	38-39	0.25783042540232515
2225	40-41	0.39431957001080775
2225	42-43	0.4585755838901804
2225	44-45	0.46811819263360377
2225	46-47	0.43562208397383273
2225	48-49	0.4445732073808273
2225	50-51	0.3663430341302032
2225	52-53	0.38254730414862337
2225	54-55	0.3584630298142599
2225	56-57	0.5839892944458995
2225	58-59	0.5038434895140895
2225	60-61	0.4149209025200591
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2225	64-65	0.44334581676876894
2225	66-67	0.46641697087653
2225	68-69	0.5596374567007629
2225	70-71	0.6077839258877944
2225	72-73	0.4593480184206342
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2225	78-79	0.5439789765192486
2225	80-81	0.5054698848708412
2225	82-83	0.5062098364248229
2225	84-85	0.3701820561653335
2225	86-87	0.3599017342907942
2225	88-89	0.43544658312332984
2225	90-91	0.44310812356903995
2225	92-93	0.4954546694911599
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2225	98-99	0.5177311696223938
2225	100	0.604759819158744
2226	1	1.4122081950328411
2226	2	1.2608684761442142
2226	3	0.7369701066437511
2226	4	0.9290817208239961
2226	5	3.232575330239303
2226	6	-0.4509887938170145
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2226	18-19	0.20320956885108643
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2226	70-71	0.5379500655041127
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2226	76-77	0.746194185738716
2226	78-79	0.613777082870314
2226	80-81	0.4245306862361957
2226	82-83	0.30686706594959645
2226	84-85	0.34043839094861994
2226	86-87	0.48987027243860837
2226	88-89	0.455611348563302
2226	90-91	0.6251978555876931
2226	92-93	0.4178475940098565
2226	94-95	0.5762287543783344
2226	96-97	0.40180186254502814
2226	98-99	0.5880743659226368
2226	100	0.6707879745051386
2227	1	1.2380560070438484
2227	2	1.734897910126838
2227	3	0.5370496944061216
2227	4	-0.1212406770608574
2227	5	3.1080811846611454
2227	6	0.04358979572596411
2227	7	0.2096120647387565
2227	8	0.34670831577020067
2227	9	0.36314658685582657
2227	10-11	0.3288523775041341
2227	12-13	0.25904318006546845
2227	14-15	0.26417381944322216
2227	16-17	0.22552140956749867
2227	18-19	0.11184011976026653
2227	20-21	0.20726121197886016
2227	22-23	0.22079600166901514
2227	24-25	0.2757910822136225
2227	26-27	0.140478535480014
2227	28-29	0.20786661882341662
2227	30-31	0.23913090553494243
2227	32-33	0.2691875355861626
2227	34-35	0.24489952764212575
2227	36-37	0.2919534064786049
2227	38-39	0.2686878886886177
2227	40-41	0.2919518737378013
2227	42-43	0.37534895348078123
2227	44-45	0.23343454049760481
2227	46-47	0.32288146711724863
2227	48-49	0.2052300155718214
2227	50-51	0.24116988342375123
2227	52-53	0.3557869301701757
2227	54-55	0.4372130458937491
2227	56-57	0.5629679439313122
2227	58-59	0.46947950607968636
2227	60-61	0.3853265186285242
2227	62-63	0.41415848122747434
2227	64-65	0.48469853846768274
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2227	68-69	0.29128022360504957
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2227	72-73	0.27354673179070943
2227	74-75	0.30324378260441165
2227	76-77	0.4935340659523888
2227	78-79	0.4827442630354213
2227	80-81	0.3483877924791159
2227	82-83	0.30555791470587224
2227	84-85	0.25021992928564174
2227	86-87	0.4120931316626937
2227	88-89	0.40467518064661334
2227	90-91	0.3732607703883204
2227	92-93	0.32759068741081876
2227	94-95	0.57415677466647
2227	96-97	0.36700042726741344
2227	98-99	0.4461198118838965
2227	100	0.5044535979875704
2228	1	1.0730146525961786
2228	2	1.7257965186177682
2228	3	-0.21925251729893702
2228	4	-0.05378581427437368
2228	5	3.2012115078876953
2228	6	0.23286209932161483
2228	7	0.3341827601319096
2228	8	0.589123878614302
2228	9	0.5119419574598041
2228	10-11	0.3965483267826073
2228	12-13	0.43235111594348297
2228	14-15	0.28841673089806363
2228	16-17	0.3633831062274808
2228	18-19	0.21046979986643777
2228	20-21	0.2638871337457118
2228	22-23	0.371915782338867
2228	24-25	0.3261234503086996
2228	26-27	0.42333397326029143
2228	28-29	0.19465943735706048
2228	30-31	0.2787473053055294
2228	32-33	0.40875294079550173
2228	34-35	0.3958048651397945
2228	36-37	0.4193808345926726
2228	38-39	0.37780462206536214
2228	40-41	0.3927393281388305
2228	42-43	0.5775941279029269
2228	44-45	0.5503071400892452
2228	46-47	0.40110361903419545
2228	48-49	0.425011722711794
2228	50-51	0.42378420125103844
2228	52-53	0.4165029570514065
2228	54-55	0.43317016261307373
2228	56-57	0.6706737524929167
2228	58-59	0.550666176957975
2228	60-61	0.5513616647225916
2228	62-63	0.5110143938193161
2228	64-65	0.568750892883898
2228	66-67	0.6562156302779556
2228	68-69	0.6992554935851132
2228	70-71	0.6827625332230056
2228	72-73	0.6278865730651191
2228	74-75	0.48500133157291003
2228	76-77	0.655584634555133
2228	78-79	0.6402541741830916
2228	80-81	0.598651005056162
2228	82-83	0.5467222453487182
2228	84-85	0.5251990663532595
2228	86-87	0.5957889089516399
2228	88-89	0.5609634725764252
2228	90-91	0.6149824357823519
2228	92-93	0.583821167100993
2228	94-95	0.9339265830754826
2228	96-97	0.6763052365497586
2228	98-99	0.836888139978349
2228	100	0.9356039134913274
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	26.0
13	50.0
14	186.0
15	677.0
16	2523.0
17	6198.0
18	10317.0
19	13315.0
20	15341.0
21	17982.0
22	20553.0
23	23578.0
24	27170.0
25	30332.0
26	34747.0
27	38834.0
28	43488.0
29	50140.0
30	56686.0
31	66455.0
32	77541.0
33	91742.0
34	113521.0
35	145456.0
36	204985.0
37	338208.0
38	832938.0
39	1575480.0
40	1734.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.22997535550369	18.793638542100794	18.77601644963591	34.200369652759605
2	19.91672361134838	50.34216288401202	12.871381755757316	16.869731748882284
3	40.250632583746224	16.582586910119073	23.21447590140417	19.952304604730532
4	17.41592827254184	15.913247294478964	22.71017964414902	43.96064478883017
5	17.55222315070323	35.55780775130898	26.30061483728855	20.58935426069924
6	17.568993097500314	28.852016416840463	30.43800028279755	23.140990202861673
7	20.34921070578821	26.63411283205601	35.837089862176555	17.179586599979217
8	21.0021709789821	28.131689913267603	33.38552675470542	17.480612353044876
9	20.91662862614294	28.299337300658323	27.786005062753194	22.998029010445542
10-11	19.837206522676016	30.177193757725828	28.680150502460418	21.30544921713774
12-13	24.097293814936346	26.874868333783397	27.389398425031175	21.638439426249082
14-15	21.7435901175016	27.571146629488076	28.72333832352092	21.961924929489403
16-17	20.657163176009185	29.56900976328595	28.47001838184075	21.30380867886411
18-19	21.317688153464804	29.235212305182824	29.853851476080823	19.59324806527155
20-21	22.612046811067017	28.73247846533113	29.90809340027077	18.747381323331084
22-23	21.758511203704593	30.274792764861647	28.625986699140643	19.34070933229311
24-25	22.004487783588527	28.768166682855046	26.62458208589494	22.60276344766149
26-27	20.31935551323719	26.6359616926501	29.86486651877518	23.17981627533753
28-29	19.865725463726815	27.94033475409563	30.654287598120096	21.539652184057463
30-31	20.28393128615469	29.864961733406687	28.251664295689594	21.59944268474903
32-33	20.852191928395452	29.780847522904384	28.86715884550895	20.49980170319121
34-35	21.44398356024408	29.102589107919556	30.017462618512614	19.43596471332375
36-37	21.88299681032487	28.254222498133565	28.721958188147866	21.1408225033937
38-39	21.60551027904114	29.356615471430487	28.901908861551256	20.135965387977116
40-41	21.142034181575198	29.658418614534526	29.773987347853815	19.42555985603646
42-43	20.496127676650257	29.725683629580846	29.27255630629751	20.505632387471383
44-45	20.550697450108757	30.25724160936284	28.773830445942572	20.41823049458583
46-47	19.965376131587366	28.287968713522517	28.885240859966686	22.861414294923435
48-49	20.94382770910809	28.33966068981249	30.041068141449813	20.67544345962961
50-51	21.917409053635968	30.51924598777721	28.077031344436737	19.486313614150085
52-53	20.449243804005164	30.161345596074312	28.33401733581727	21.05539326410325
54-55	22.326794627869393	28.389142180705807	28.057701047937915	21.226362143486885
56-57	21.530468394547224	28.72404148934314	29.287322842499723	20.45816727360991
58-59	21.308556550700157	27.991274188772238	28.994661743292284	21.705507517235326
60-61	19.711100532941824	30.371004694033687	29.35079581916955	20.567098953854934
62-63	20.5027050472991	31.010804953894983	27.50477144899306	20.981718549812857
64-65	19.939922368586203	30.206997139210007	28.688637929606752	21.16444256259703
66-67	20.03041245475825	30.214366271783028	29.45749222111435	20.29772905234437
68-69	20.920990374727584	29.837237771024082	29.146232634056062	20.095539220192265
70-71	19.799317900642233	30.801874275917186	29.13487906759096	20.263928755849626
72-73	20.091164451462593	30.49341402004009	28.667338679752085	20.748082848745238
74-75	19.973358179242087	29.41252064018491	30.202309617486367	20.411811563086637
76-77	19.604757873477002	30.673456585498215	28.546095089243977	21.175690451780802
78-79	19.735388988550863	29.453638258185833	28.505758679944783	22.30521407331852
80-81	20.960184990485516	29.513716416929075	28.451961129603358	21.074137462982048
82-83	20.502900497708062	28.193587682734478	29.51832233868887	21.78518948086859
84-85	21.189895430007216	28.730030678065717	28.464068175562595	21.616005716364473
86-87	21.573299640670037	29.063437531817982	29.227387198020523	20.135875629491462
88-89	22.152696094451258	28.192897615047958	29.249456343844322	20.404949946656465
90-91	21.64416829006175	28.176388071151447	28.889709736698816	21.28973390208799
92-93	22.04244150634745	27.990577581445564	29.268166292250697	20.698814619956288
94-95	21.17877622615263	28.650959337305864	29.13776953978735	21.032494896754155
96-97	21.168451251144795	28.573997260040677	28.697076690997847	21.560474797816678
98-99	22.90983054802051	28.86735414768438	28.229783685914523	19.99303161838059
100	21.797910162561717	30.441880286016133	27.794520237602022	19.96568931382013
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	11.5
2	18.0
3	23.5
4	36.0
5	39.5
6	39.5
7	52.5
8	71.5
9	87.5
10	111.5
11	137.5
12	149.0
13	171.0
14	191.5
15	221.5
16	324.0
17	469.0
18	633.0
19	1007.0
20	1654.0
21	2566.5
22	4015.5
23	6204.5
24	9350.0
25	13920.5
26	19951.5
27	27216.5
28	35119.5
29	44240.0
30	54990.0
31	66916.5
32	80637.0
33	96195.5
34	112554.5
35	130365.0
36	147813.5
37	160584.5
38	169892.0
39	178155.0
40	184348.5
41	186898.5
42	186103.5
43	183222.0
44	180375.0
45	175362.5
46	162868.0
47	148378.0
48	139707.5
49	136043.0
50	131847.5
51	121259.0
52	105150.5
53	88934.5
54	74880.0
55	61221.5
56	47620.5
57	35570.5
58	25711.0
59	18410.5
60	14211.5
61	12047.0
62	9870.0
63	7450.5
64	5539.0
65	4576.0
66	4503.0
67	4103.0
68	3128.0
69	2887.5
70	3361.5
71	3267.5
72	2076.5
73	906.5
74	425.0
75	268.5
76	193.0
77	147.5
78	119.5
79	101.5
80	94.0
81	70.0
82	50.5
83	42.5
84	30.5
85	29.0
86	27.0
87	23.5
88	23.5
89	25.0
90	17.0
91	13.5
92	19.5
93	21.5
94	21.0
95	20.5
96	22.5
97	23.5
98	57.0
99	139.0
100	146.5
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.337577727010785
2	0.005702823522610654
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	2.8644319063341183E-4
30-31	0.009517726016046548
32-33	0.0
34-35	0.0
36-37	0.0
38-39	8.983900069866099E-4
40-41	0.0016665785636853052
42-43	5.208058011516579E-5
44-45	0.0
46-47	0.0020181224794626742
48-49	0.0
50-51	0.0
52-53	0.0015884576935125563
54-55	0.019738539863647833
56-57	0.01223893632706396
58-59	0.01458256243224642
60-61	2.3436261051824601E-4
62-63	0.011366586610134934
64-65	1.3020145028791447E-4
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.4322159531670591E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3840203.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.7663169715361
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.20981816198234	62.0040463746176
2	9.152152341763212	13.319368365450565
3	2.4906614631496833	5.437087844890192
4	1.0959487269343602	3.1899260979462842
5	0.5941580928414524	2.1617348057452244
6	0.3620832807803928	1.580848006761586
7	0.24303910391568462	1.2379542330404765
8	0.16890035872829345	0.9832205631863324
9	0.11942149576556786	0.7820876172683041
>10	0.5282062106066892	6.521060755620528
>50	0.02495801606986478	1.2469586116073332
>100	0.010107668772676775	1.2617915070974781
>500	4.724015400970988E-4	0.21817496944541814
>1k	7.267714971285299E-5	0.05574024732273235
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	1.3020145028791447E-5	0.0	0.0
28-29	0.0	0.0	2.6040290057582894E-5	0.0	0.0
30-31	0.0	0.0	2.6040290057582894E-5	0.0	0.0
32-33	0.0	0.0	2.6040290057582894E-5	2.6040290057582894E-5	0.0
34-35	0.0	0.0	2.6040290057582894E-5	2.6040290057582894E-5	0.0
36-37	0.0	0.0	2.6040290057582894E-5	2.6040290057582894E-5	0.0
38-39	0.0	0.0	2.6040290057582894E-5	2.6040290057582894E-5	0.0
40-41	0.0	0.0	2.6040290057582894E-5	2.6040290057582894E-5	0.0
42-43	0.0	0.0	2.6040290057582894E-5	3.906043508637434E-5	0.0
44-45	1.3020145028791447E-5	0.0	2.6040290057582894E-5	5.208058011516579E-5	0.0
46-47	2.6040290057582894E-5	0.0	2.6040290057582894E-5	5.208058011516579E-5	0.0
48-49	2.6040290057582894E-5	0.0	2.6040290057582894E-5	5.208058011516579E-5	0.0
50-51	2.6040290057582894E-5	0.0	2.6040290057582894E-5	1.0416116023033157E-4	0.0
52-53	3.906043508637434E-5	0.0	2.6040290057582894E-5	1.0416116023033157E-4	0.0
54-55	6.510072514395723E-5	0.0	2.6040290057582894E-5	1.0416116023033157E-4	0.0
56-57	1.0416116023033157E-4	0.0	2.6040290057582894E-5	1.3020145028791447E-4	0.0
58-59	1.692618853742888E-4	0.0	2.6040290057582894E-5	1.3020145028791447E-4	0.0
60-61	2.3436261051824604E-4	0.0	2.6040290057582894E-5	1.3020145028791447E-4	0.0
62-63	2.3436261051824604E-4	0.0	2.6040290057582894E-5	1.3020145028791447E-4	0.0
64-65	3.255036257197862E-4	0.0	2.6040290057582894E-5	1.3020145028791447E-4	0.0
66-67	4.426849309789092E-4	0.0	2.6040290057582894E-5	1.3020145028791447E-4	0.0
68-69	4.947655110940749E-4	0.0	2.6040290057582894E-5	1.3020145028791447E-4	0.0
70-71	5.208058011516579E-4	0.0	2.6040290057582894E-5	1.3020145028791447E-4	0.0
72-73	5.468460912092407E-4	0.0	2.6040290057582894E-5	1.3020145028791447E-4	0.0
74-75	5.989266713244065E-4	0.0	2.6040290057582894E-5	1.3020145028791447E-4	0.0
76-77	6.11946816353198E-4	0.0	2.6040290057582894E-5	1.3020145028791447E-4	0.0
78-79	7.030878315547381E-4	0.0	2.6040290057582894E-5	1.3020145028791447E-4	0.0
80-81	0.00108067203738969	0.0	2.6040290057582894E-5	1.3020145028791447E-4	0.0
82-83	0.0014061756631094761	0.0	2.6040290057582894E-5	1.3020145028791447E-4	0.0
84-85	0.0015233569683685993	0.0	2.6040290057582894E-5	1.3020145028791447E-4	0.0
86-87	0.0015363771133973907	1.0416116023033157E-4	2.6040290057582894E-5	1.3020145028791447E-4	0.0
88	0.0015363771133973907	1.0416116023033157E-4	2.6040290057582894E-5	1.3020145028791447E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCTGCG	40	4.0563464E-10	92.5936	1
TCACCGG	13730	0.0	80.20229	8
CACCGGC	13390	0.0	78.69825	9
CTTCACC	16115	0.0	70.42961	6
CCTTCAC	17535	0.0	68.446976	5
CGGGGCA	1820	0.0	67.82873	94
CGTAGTG	3100	0.0	66.77382	4
TTCACCG	17155	0.0	65.74949	7
GTGTGGC	5980	0.0	65.38425	7
GGCCCGA	930	0.0	62.584682	9
CAGCTGC	120	0.0	61.72906	1
AACGGGC	970	0.0	60.971676	5
ACCTTCA	7095	0.0	58.87988	4
CGGGCCC	1035	0.0	58.04956	7
ATGCAGC	90	0.0	57.37228	2
GCGTAGT	2095	0.0	56.908745	3
GGCGCGC	440	0.0	56.53957	6
AGGCTGC	50	4.6063178E-7	56.32915	2
TGTGGCA	6945	0.0	56.29918	8
TGTGTGG	6640	0.0	55.56276	6
>>END_MODULE