FastQCFastQC Report
Tue 7 Mar 2017
1SN_31A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_31A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3353715
Sequences flagged as poor quality0
Sequence length93
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA181320.5406541700770637No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG103670.30911988645427535No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA77290.2304608471501007No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT70090.2089921176963457No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG66210.1974228579351555No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA63500.1893422667102005No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG59960.17878680806210426No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG58590.17470178592993144No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT54920.16375869744447574No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC53590.15979294603149047No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA52360.15612537141647398No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA48880.14574881884715904No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT47690.1422005149513301No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA47460.1415147083160018No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC43460.12958763639724902No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG40460.12064233245818444No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA39720.11843582415321516No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC38520.11485770257758934No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA35640.10627021079608734No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA35060.10454078536786818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTA257.803992E-786.99993
GCTGCAT257.803992E-786.99993
GCAGCCT257.803992E-786.99993
GCAGCAA950.086.99993
TGCAGCG257.803992E-786.99992
TGCTGCC402.0008883E-1186.999892
AGCTGCG800.086.999892
AGCTGCC1700.084.441082
CAGCTGC5000.084.389891
CAGCAGC5250.083.6856161
CTGCAGC1300.083.653751
GCTGCCC1250.083.51993
AGCAGCC1100.083.045362
AGCAGCA1900.082.420962
GCTGCAA800.081.56243
CAGCCCT196350.081.417021
CTGCTGC1300.080.30761
CAGCGTA79750.080.290821
AGCAGCG1250.080.03992
AGCCCTT167450.079.492292
Produced by FastQC (version 0.11.5)