FastQCFastQC Report
Tue 7 Mar 2017
1SN_31A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_31A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3353715
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG133200.079.9578
CACCGGC131700.078.016469
CTTCACC147000.075.069166
CCTTCAC164650.070.699485
CGTAGTG31200.068.601964
GTGTGGC53600.068.3931357
GGGCCCG8300.067.8624348
CAGCGTA16000.067.801791
TTCACCG164050.066.9523547
CGGGGCA14550.066.4554394
CAGCTGC900.064.678131
CGGGCCC8800.064.006617
GCCTTCA47300.063.113494
ACCTTCA63950.062.97574
GCGTAGT21250.062.2897263
GGCCCGA9300.059.555979
TGTGGCA61600.058.444138
AGCGTAG19000.057.5670432
TGCTGCA1200.057.3283421
GGCGCGC3850.057.3011556
Produced by FastQC (version 0.11.5)