FastQCFastQC Report
Tue 7 Mar 2017
1SN_32A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_32A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2552635
Sequences flagged as poor quality0
Sequence length93
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA141920.555974512611478No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG77160.3022758835477849No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA55270.21652135930127106No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG52100.2041028192436443No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT51480.2016739565194397No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA49690.19466159478342968No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT44080.17268430464990098No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG43910.17201832616100618No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG41810.16379153306289382No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC39460.15458535983405383No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA38060.14910083110197894No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT35160.13774002158553808No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA35070.1373874447384761No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA34700.1359379621449992No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG31200.12222664031481195No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA30510.11952355115400361No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA30100.11791736773961024No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC28020.10976892505195612No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC26230.10275656331594607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCA500.086.999323
GCTGCAC450.086.999323
TGCTGCA500.086.999322
AGCTGCC2400.086.999312
GCAGCCT159.388723E-486.999313
GCAGCCG302.2702807E-886.999313
GCAGCCC202.6992644E-586.999313
AGCAGCT950.086.999312
CAGCTGC6000.086.274321
GCTGCCC1500.084.0993353
AGCTGCT1400.083.89222
AGCAGCC1050.082.856492
CAGCAGC4600.082.271091
AGCTGCA1700.081.881712
GCTGCAA800.081.561863
CAGCCCT149750.081.1896741
GCTGCAT700.080.785073
CAGCGTA61300.080.612741
AGCTGCG650.080.307062
CTGCTGC1250.080.039371
Produced by FastQC (version 0.11.5)