##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1SN_32A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2552635
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.789251107189237	12.0	12.0	12.0	2.0	27.0
2	14.636185745318073	12.0	12.0	12.0	12.0	22.0
3	13.735743653127063	12.0	12.0	12.0	12.0	22.0
4	13.731596957653561	12.0	12.0	12.0	12.0	22.0
5	22.42927210509924	22.0	22.0	22.0	22.0	27.0
6	26.74596485592339	27.0	22.0	32.0	22.0	32.0
7	34.93436390239889	37.0	37.0	37.0	27.0	41.0
8	37.53628152869486	41.0	37.0	41.0	27.0	41.0
9	38.50647193977987	41.0	37.0	41.0	32.0	41.0
10-11	38.86929486589348	41.0	41.0	41.0	37.0	41.0
12-13	39.05460181342025	41.0	41.0	41.0	37.0	41.0
14-15	39.00633149666913	41.0	41.0	41.0	37.0	41.0
16-17	38.90778195864274	41.0	41.0	41.0	37.0	41.0
18-19	38.84523639298216	41.0	41.0	41.0	37.0	41.0
20-21	38.857143108983465	41.0	41.0	41.0	37.0	41.0
22-23	38.84163051121684	41.0	41.0	41.0	34.5	41.0
24-25	38.86532622172774	41.0	41.0	41.0	37.0	41.0
26-27	38.828765569695626	41.0	41.0	41.0	37.0	41.0
28-29	38.78395364006213	41.0	41.0	41.0	34.5	41.0
30-31	38.65748902604564	41.0	41.0	41.0	32.0	41.0
32-33	38.7042657880974	41.0	41.0	41.0	32.0	41.0
34-35	38.67931960503558	41.0	41.0	41.0	32.0	41.0
36-37	38.60034767994641	41.0	41.0	41.0	32.0	41.0
38-39	38.50171391522878	41.0	41.0	41.0	32.0	41.0
40-41	38.555417245317095	41.0	41.0	41.0	32.0	41.0
42-43	38.28399144413518	41.0	41.0	41.0	32.0	41.0
44-45	38.40137309094328	41.0	41.0	41.0	32.0	41.0
46-47	38.42484315227206	41.0	41.0	41.0	32.0	41.0
48-49	38.36112957786757	41.0	41.0	41.0	32.0	41.0
50-51	38.23103303057429	41.0	41.0	41.0	32.0	41.0
52-53	38.21067935682148	41.0	41.0	41.0	32.0	41.0
54-55	38.07745251475436	41.0	37.0	41.0	32.0	41.0
56-57	37.86781208437556	41.0	37.0	41.0	27.0	41.0
58-59	37.86697608549597	41.0	37.0	41.0	29.5	41.0
60-61	37.86135777343803	41.0	37.0	41.0	29.5	41.0
62-63	37.850047108184285	41.0	37.0	41.0	29.5	41.0
64-65	37.83266311086388	41.0	37.0	41.0	27.0	41.0
66-67	37.712763281863644	41.0	37.0	41.0	27.0	41.0
68-69	37.71604714344197	41.0	37.0	41.0	27.0	41.0
70-71	37.7101348214688	41.0	37.0	41.0	27.0	41.0
72-73	37.56660019940179	41.0	37.0	41.0	27.0	41.0
74-75	37.47805150364232	41.0	37.0	41.0	27.0	41.0
76-77	36.439172267088715	39.0	34.5	41.0	24.5	41.0
78-79	36.954276071588765	41.0	37.0	41.0	27.0	41.0
80-81	37.600948823470645	41.0	37.0	41.0	27.0	41.0
82-83	37.797254601617546	41.0	37.0	41.0	27.0	41.0
84-85	37.70712401107092	41.0	37.0	41.0	27.0	41.0
86-87	37.550725426862826	41.0	37.0	41.0	27.0	41.0
88-89	37.44985436617456	41.0	37.0	41.0	27.0	41.0
90-91	37.294804192530464	41.0	37.0	41.0	27.0	41.0
92-93	37.171220914858566	41.0	37.0	41.0	27.0	41.0
94-95	37.01461959896342	41.0	37.0	41.0	27.0	41.0
96-97	36.96621021023374	41.0	37.0	41.0	27.0	41.0
98-99	36.85367767032889	41.0	37.0	41.0	27.0	41.0
100	36.73003034119645	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.5941514418715705
1101	2	-0.6015171272723645
1101	3	-0.040311312299674995
1101	4	0.008832851663239438
1101	5	-1.045672347680675
1101	6	-0.8920068640650065
1101	7	-0.456926542277003
1101	8	-0.14605788026509714
1101	9	-0.11695259598344165
1101	10-11	-0.15998090373862794
1101	12-13	-0.11707710964282825
1101	14-15	-0.009498353400545057
1101	16-17	-0.21583870987424802
1101	18-19	0.004378714225779845
1101	20-21	-0.12004933853162925
1101	22-23	-0.05883489693330546
1101	24-25	-0.034507558462763654
1101	26-27	-0.06934075324414124
1101	28-29	0.0869504327440751
1101	30-31	-0.3430671884767804
1101	32-33	-0.1379322466251054
1101	34-35	0.04978437261151214
1101	36-37	-0.047341007070215824
1101	38-39	-0.11191160279517476
1101	40-41	-0.2010805855746156
1101	42-43	0.05639344893464937
1101	44-45	-0.0014019744880471308
1101	46-47	0.0972895276273249
1101	48-49	0.08714069743321318
1101	50-51	0.06465537181491499
1101	52-53	0.08034542024378055
1101	54-55	-0.10729014734537401
1101	56-57	0.09763272041051607
1101	58-59	-5.636258455368193E-4
1101	60-61	0.03328515522048292
1101	62-63	-0.010715133128520904
1101	64-65	0.059497503856540845
1101	66-67	0.008028286656674766
1101	68-69	-0.0801069224604376
1101	70-71	-0.07692015192092327
1101	72-73	-0.0872696369856385
1101	74-75	-0.22025526752758395
1101	76-77	-0.482170380686604
1101	78-79	-0.35835137963587727
1101	80-81	-0.1566582992995862
1101	82-83	-0.17824118797280875
1101	84-85	-0.1795185905264205
1101	86-87	-0.02123546098598439
1101	88-89	-0.4025956623340221
1101	90-91	-0.18921826008429576
1101	92-93	-0.13069179930855768
1101	94-95	0.01855917283526054
1101	96-97	-0.17109150160940345
1101	98-99	-0.11245638417504011
1101	100	-0.6049887569857972
1102	1	1.6295893030208024
1102	2	0.6381378799377515
1102	3	-0.03602523261541535
1102	4	0.09735475006592864
1102	5	-0.7678403477503615
1102	6	-0.23861767377573884
1102	7	0.003929176996457784
1102	8	-0.17910880087146097
1102	9	-0.10512598562510789
1102	10-11	-0.23224796867084763
1102	12-13	-0.07055384286218924
1102	14-15	-0.11461074569423602
1102	16-17	-0.1939102155413721
1102	18-19	0.016602851050905088
1102	20-21	0.014437052521969918
1102	22-23	0.044222156825419745
1102	24-25	-0.1767574949139643
1102	26-27	-0.12405072917874804
1102	28-29	-0.2863969024427959
1102	30-31	-0.13760302089482934
1102	32-33	-0.1508005822166325
1102	34-35	0.05445926802324408
1102	36-37	-0.10326167678353215
1102	38-39	-0.06551949203257834
1102	40-41	0.041527857190686746
1102	42-43	0.04881461080473315
1102	44-45	0.03632760298566495
1102	46-47	-0.345724914778458
1102	48-49	-0.333226515660769
1102	50-51	-0.10569105428753289
1102	52-53	-0.0928998013217992
1102	54-55	-0.1417968502592757
1102	56-57	0.0071650606139854744
1102	58-59	0.041705335652203246
1102	60-61	0.07257425198808676
1102	62-63	-0.051967808711289365
1102	64-65	-0.1415834790949546
1102	66-67	-0.17399300476586177
1102	68-69	-0.34086260681174707
1102	70-71	-0.35501872027132464
1102	72-73	-0.09874366037318083
1102	74-75	-0.387374859643252
1102	76-77	-0.14605611083265302
1102	78-79	-0.1728595514971829
1102	80-81	-0.316692991798611
1102	82-83	-0.20378879651852344
1102	84-85	-0.20892030527505057
1102	86-87	-0.2575723694179004
1102	88-89	-0.1994605286756439
1102	90-91	-0.3963180669434436
1102	92-93	-0.2407295207692144
1102	94-95	0.007720241532105376
1102	96-97	-8.277285447562122E-4
1102	98-99	-0.23574086345892198
1102	100	-0.4341111737566621
1103	1	1.2296892952418297
1103	2	0.7231555771023164
1103	3	0.43732124934384764
1103	4	1.0917141840159612
1103	5	2.7261463437225864
1103	6	-0.036220939648085704
1103	7	-0.3170847473659961
1103	8	-0.44010875549411566
1103	9	-0.36189936468151984
1103	10-11	-0.2868555518734581
1103	12-13	-0.5180194371109295
1103	14-15	-0.3569553444936773
1103	16-17	-0.45504346611507174
1103	18-19	-0.4831500044805921
1103	20-21	-0.2748816066423103
1103	22-23	-0.20564697815273547
1103	24-25	-0.14644542842967212
1103	26-27	-0.015734196267203515
1103	28-29	-0.18331578071482824
1103	30-31	-0.26467457772670855
1103	32-33	-0.45854487439487457
1103	34-35	-0.6152815787180117
1103	36-37	-0.40776270425627104
1103	38-39	-0.2734194447104912
1103	40-41	-0.37885151362001324
1103	42-43	-0.2659684368674178
1103	44-45	-0.7393033041722887
1103	46-47	-0.6162346644251642
1103	48-49	-0.3553260930032138
1103	50-51	-0.250651413935401
1103	52-53	-0.3154088281722167
1103	54-55	-0.491025759517548
1103	56-57	-0.40761912149178414
1103	58-59	-0.6144968560735649
1103	60-61	-0.6936983880658474
1103	62-63	-0.5077775674334646
1103	64-65	-0.5603041458826326
1103	66-67	-0.5223848640701618
1103	68-69	-0.49939362870956217
1103	70-71	-0.6593828053992254
1103	72-73	-0.622812290366312
1103	74-75	-0.5611149900707133
1103	76-77	-0.6007529091368369
1103	78-79	-0.7762907270297177
1103	80-81	-0.46957372392966334
1103	82-83	-0.6388457126741542
1103	84-85	-0.4946622572787831
1103	86-87	-0.47091274164177577
1103	88-89	-0.5932625505604889
1103	90-91	-0.7532983991056099
1103	92-93	-0.4250676921918526
1103	94-95	-0.4860853457155585
1103	96-97	-0.3962098544085464
1103	98-99	-0.5193415719937562
1103	100	-0.5918922539967753
1104	1	1.574819232602703
1104	2	-0.6486930536374462
1104	3	-0.04570535934280073
1104	4	-0.02334961988733575
1104	5	-1.1240406677181127
1104	6	-0.7270826158918098
1104	7	-0.6018727579266425
1104	8	-0.1659849267422402
1104	9	-0.0504023672181404
1104	10-11	-0.13627575169743977
1104	12-13	0.019276079791275436
1104	14-15	-0.12323310501550111
1104	16-17	-0.09010099316378728
1104	18-19	-0.17372048643949256
1104	20-21	-0.0810804858678722
1104	22-23	-0.016720126157082404
1104	24-25	-0.12121506139546057
1104	26-27	-0.15829806488034137
1104	28-29	-0.1299091311315621
1104	30-31	-0.16223242832462859
1104	32-33	-0.1686027249927946
1104	34-35	-0.2856981806748351
1104	36-37	-0.2929642107013706
1104	38-39	-0.2668724265399689
1104	40-41	-0.06080749798611862
1104	42-43	-0.13550854402691215
1104	44-45	-0.18008247536864985
1104	46-47	-0.44359851491954316
1104	48-49	-0.3882666376012338
1104	50-51	-0.3268715179888204
1104	52-53	-0.29745831825866986
1104	54-55	-0.21747623060519317
1104	56-57	-0.3501131034765663
1104	58-59	-0.28193842308707673
1104	60-61	-0.17929721293513978
1104	62-63	-0.21048923332360658
1104	64-65	-0.22809838152998907
1104	66-67	-0.5528146668345713
1104	68-69	-0.6276533869535896
1104	70-71	-0.36408591664838497
1104	72-73	-0.27555339923414834
1104	74-75	-0.43873897412620266
1104	76-77	-0.57653201562335
1104	78-79	-0.39364202381493385
1104	80-81	-0.4517073368095055
1104	82-83	-0.46414208374626753
1104	84-85	-0.4522611339159468
1104	86-87	-0.3301154516899274
1104	88-89	-0.3280298539719979
1104	90-91	-0.33318269790976984
1104	92-93	-0.6311086794051874
1104	94-95	-0.41765145328920283
1104	96-97	-0.5297822906046363
1104	98-99	-0.6541612903765284
1104	100	-0.4381934394810614
1105	1	1.466664394732776
1105	2	0.3690723641165423
1105	3	0.01579779627286193
1105	4	-0.20126173884833243
1105	5	-1.1780258229909109
1105	6	0.5594557784903031
1105	7	0.19130663596657627
1105	8	0.08421547390031492
1105	9	0.21221771443420323
1105	10-11	0.0106750632387147
1105	12-13	-0.04154908671982582
1105	14-15	-0.011641098501094405
1105	16-17	-0.10611756808268069
1105	18-19	-0.11626978896589435
1105	20-21	0.04473877576886309
1105	22-23	0.09443972926873556
1105	24-25	-0.06232901912163413
1105	26-27	-0.14131619379708127
1105	28-29	0.006949626495575956
1105	30-31	-0.05891557943226644
1105	32-33	-0.21336667287751254
1105	34-35	-0.0963785031571689
1105	36-37	-0.1979975208596585
1105	38-39	-0.08549040021566157
1105	40-41	-0.004283008630764584
1105	42-43	0.09178626158843173
1105	44-45	-0.11933707228160273
1105	46-47	-0.34712921709165556
1105	48-49	-0.1858373849805517
1105	50-51	-0.25176353974161714
1105	52-53	-0.17742170645019684
1105	54-55	-0.2305179472728085
1105	56-57	-0.19187794661643665
1105	58-59	-0.0382492575791602
1105	60-61	-0.14016115002600316
1105	62-63	-0.44406292837812344
1105	64-65	-0.24697770939020103
1105	66-67	-0.3194506979970626
1105	68-69	-0.30404523886188173
1105	70-71	-0.31403833637700984
1105	72-73	-0.2744498067847658
1105	74-75	-0.3704999759230603
1105	76-77	-0.2520581764391139
1105	78-79	-0.3009331150738106
1105	80-81	-0.42371374804274353
1105	82-83	-0.25409919404042824
1105	84-85	-0.27208230511285336
1105	86-87	-0.1786970913402044
1105	88-89	-0.12079634317495902
1105	90-91	-0.40681505281177266
1105	92-93	-0.2476099064126842
1105	94-95	-0.22566228047956116
1105	96-97	-0.3959948216369753
1105	98-99	-0.4998168822290694
1105	100	-0.35513034939045696
1106	1	1.4053519710864268
1106	2	-0.636261504852941
1106	3	-0.2250207384118017
1106	4	-0.2991474145305819
1106	5	-1.1773098542597182
1106	6	-0.6544050622962949
1106	7	-0.2172227681826655
1106	8	-0.17711232836207103
1106	9	-0.36699118131054576
1106	10-11	-0.17249778848239572
1106	12-13	-0.12525485456392715
1106	14-15	-0.12245050017092751
1106	16-17	-0.1472616429608422
1106	18-19	-0.026022420788486045
1106	20-21	-0.17537747401438253
1106	22-23	-0.2649993278651763
1106	24-25	-0.302621660061547
1106	26-27	-0.23808005436325175
1106	28-29	-0.27926583733054855
1106	30-31	-0.07449152821833849
1106	32-33	-0.24291813514066973
1106	34-35	-0.11522260400636952
1106	36-37	-0.37550833492983315
1106	38-39	-0.1023913815439812
1106	40-41	-0.13168170003608282
1106	42-43	-0.012534109506979974
1106	44-45	-0.09641057657388075
1106	46-47	-0.27770182730571946
1106	48-49	-0.29829174932978475
1106	50-51	-0.20784856779582128
1106	52-53	-0.2294598830798762
1106	54-55	-0.413705314524357
1106	56-57	-0.3363478825990214
1106	58-59	-0.1795051805040444
1106	60-61	-0.244654138240179
1106	62-63	-0.43822219277811314
1106	64-65	-0.6098491010058709
1106	66-67	-0.4546295175627435
1106	68-69	-0.4010072168029879
1106	70-71	-0.5309244883255886
1106	72-73	-0.36676328418015913
1106	74-75	-0.3077652264493551
1106	76-77	-0.306727587194878
1106	78-79	-0.2699846310836591
1106	80-81	-0.29245667172165213
1106	82-83	-0.4043486650081931
1106	84-85	-0.507485958475705
1106	86-87	-0.5058979399887278
1106	88-89	-0.548449566368113
1106	90-91	-0.5502398706026312
1106	92-93	-0.519965000226172
1106	94-95	-0.5431294851885511
1106	96-97	-0.41369415713953117
1106	98-99	-0.5937266756613013
1106	100	-0.6081128985843733
1107	1	1.3747137384879178
1107	2	-0.5720636256915643
1107	3	-0.23286384846123553
1107	4	-0.1599890592481028
1107	5	-1.1821910822093997
1107	6	-0.3036453790423188
1107	7	-0.4869637027422229
1107	8	-0.3425472220696193
1107	9	-0.26936932019255977
1107	10-11	-0.11776983841313893
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2221	2	2.004621778308282
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2221	5	-0.7213425486925189
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2221	78-79	0.5571304091175477
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2222	9	0.14731094710517567
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2222	12-13	0.18845380516965093
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2222	18-19	0.09971301505937191
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2222	24-25	0.08216470024818534
2222	26-27	0.12509970144267868
2222	28-29	0.25470783530844443
2222	30-31	0.15676342237362917
2222	32-33	0.10174621848896948
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2222	36-37	0.15644218324467118
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2222	46-47	0.2018463925894025
2222	48-49	0.25902443500240224
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2222	56-57	0.3678303417234119
2222	58-59	0.39257901494757874
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2222	62-63	0.154062112000652
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2222	78-79	0.5399325905010812
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2222	84-85	0.16734203198301145
2222	86-87	0.20119623947041987
2222	88-89	0.25844515882320707
2222	90-91	0.4031812707968143
2222	92-93	0.4533373796720994
2222	94-95	0.4335654619699909
2222	96-97	0.3661789947649581
2222	98-99	0.32947684298605395
2222	100	0.6298998923899646
2223	1	1.3731788744696516
2223	2	-0.9584827385477599
2223	3	-0.00479597251927899
2223	4	0.045468066187138945
2223	5	-1.0886869002748263
2223	6	0.17039471358140545
2223	7	0.22289058015525143
2223	8	0.2619570719404223
2223	9	0.2083468477382766
2223	10-11	0.2525618859723977
2223	12-13	0.10178387278486412
2223	14-15	0.10639587572986642
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2223	18-19	0.008581759168095004
2223	20-21	0.058744104760627636
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2223	24-25	0.1312484436517991
2223	26-27	0.1256247212967665
2223	28-29	0.08447717541072564
2223	30-31	0.07513689064049345
2223	32-33	0.1061990993718922
2223	34-35	0.09674891504686656
2223	36-37	-0.0745404824719742
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2223	40-41	0.0787611875828702
2223	42-43	0.23780150606270212
2223	44-45	0.27062224484948416
2223	46-47	0.23709592314242656
2223	48-49	0.3291123125082862
2223	50-51	0.35646478319218744
2223	52-53	0.16514632230624926
2223	54-55	0.09393112276398341
2223	56-57	0.22447273816674596
2223	58-59	0.20916320367640395
2223	60-61	0.17079373723565539
2223	62-63	0.20409773801655007
2223	64-65	0.15315841579856482
2223	66-67	0.3228034113787501
2223	68-69	0.3418047797973216
2223	70-71	0.30030498527095517
2223	72-73	0.3761359762541119
2223	74-75	0.21610474475926367
2223	76-77	0.2936113409932304
2223	78-79	0.3813120224145621
2223	80-81	0.18797020175336598
2223	82-83	0.13438701264941955
2223	84-85	0.18778106696330354
2223	86-87	0.2783992075628703
2223	88-89	0.14240760677583353
2223	90-91	0.3487471306826606
2223	92-93	0.4587511424473618
2223	94-95	0.5791318785123352
2223	96-97	0.4930433301542152
2223	98-99	0.6091016379509071
2223	100	0.45010002895635637
2224	1	1.3200556134135066
2224	2	-0.9096178267800035
2224	3	-0.02945950162035693
2224	4	-0.20807584243990895
2224	5	-1.100410329212739
2224	6	0.6719584662104516
2224	7	0.18469464893018284
2224	8	-0.00933986185945912
2224	9	0.22209523258364783
2224	10-11	0.20023087594664446
2224	12-13	0.143195022774961
2224	14-15	0.11495501632612815
2224	16-17	0.05798465519943363
2224	18-19	-0.05763722278933159
2224	20-21	0.05913928711983374
2224	22-23	0.024302938973455923
2224	24-25	0.18869466018155379
2224	26-27	0.07671831066551249
2224	28-29	0.001090396571377994
2224	30-31	0.061695031491410646
2224	32-33	0.07429307543459629
2224	34-35	0.07410798396328033
2224	36-37	0.0029808092744758596
2224	38-39	0.11542107898822707
2224	40-41	0.13672378201636093
2224	42-43	0.31642864820573635
2224	44-45	0.1702355508941693
2224	46-47	0.14406678905393733
2224	48-49	0.09619711166321565
2224	50-51	0.09579816053946644
2224	52-53	0.21557262881308503
2224	54-55	0.2927182241626909
2224	56-57	0.3868875004442742
2224	58-59	0.37878697875256506
2224	60-61	0.300146637911908
2224	62-63	0.3717522033885814
2224	64-65	0.18246132920741331
2224	66-67	0.2589083657771596
2224	68-69	0.3230020220092129
2224	70-71	0.4402528287447822
2224	72-73	0.330781962653802
2224	74-75	0.1021073667329162
2224	76-77	0.35942005009562905
2224	78-79	0.2593195110731159
2224	80-81	0.25694131288543076
2224	82-83	0.26263406292907376
2224	84-85	0.15187417532249725
2224	86-87	0.2677580501218415
2224	88-89	0.29726533169495184
2224	90-91	0.2971829064756193
2224	92-93	0.29415863299222167
2224	94-95	0.39033327061057577
2224	96-97	0.16847175876201703
2224	98-99	0.23660123710961756
2224	100	0.6696044947999624
2225	1	1.3085982401305785
2225	2	1.5230165738898673
2225	3	0.6150678038897972
2225	4	0.9554784246388497
2225	5	3.196412187366395
2225	6	-0.10318289472506592
2225	7	0.21020304052919414
2225	8	0.3003121535997053
2225	9	-0.021062246660100925
2225	10-11	0.152883632939961
2225	12-13	0.1705848417218263
2225	14-15	0.10487091830566442
2225	16-17	0.18764171002505492
2225	18-19	-0.037694552768201106
2225	20-21	0.024730569590701634
2225	22-23	0.20963282586554755
2225	24-25	0.2016102881575108
2225	26-27	0.06719831511088614
2225	28-29	0.19352665241790135
2225	30-31	0.1847174633785258
2225	32-33	0.11133586789509309
2225	34-35	0.14965776351494497
2225	36-37	0.07848900926259716
2225	38-39	0.10721965378641585
2225	40-41	0.02551741900185789
2225	42-43	0.20612132349616985
2225	44-45	0.16791265205130657
2225	46-47	0.22972739634550265
2225	48-49	0.1732890714785782
2225	50-51	0.26694558404361146
2225	52-53	0.13120414957125348
2225	54-55	0.10932399050087582
2225	56-57	0.3787065776078933
2225	58-59	0.30801856159265384
2225	60-61	0.19976878123301844
2225	62-63	0.1281158885259046
2225	64-65	0.10564164846947932
2225	66-67	0.28330349809406385
2225	68-69	0.4052431413234032
2225	70-71	0.19771262398938916
2225	72-73	0.3162591025163408
2225	74-75	0.24687361102530048
2225	76-77	0.30335719405864126
2225	78-79	0.37921351192770203
2225	80-81	0.289549462863107
2225	82-83	0.22209542555076922
2225	84-85	0.11585190247242139
2225	86-87	0.19468254201765944
2225	88-89	0.23226229466231985
2225	90-91	0.3871068561126165
2225	92-93	0.3801814806749775
2225	94-95	0.4825183245777893
2225	96-97	0.43037232803798275
2225	98-99	0.5641357222798931
2225	100	0.39117853995498564
2226	1	1.505820109860716
2226	2	1.3855261867312496
2226	3	0.9133554185750086
2226	4	1.1141667514769846
2226	5	3.1718516643055104
2226	6	-0.46673004466093104
2226	7	0.29723021486600487
2226	8	0.32031718361928796
2226	9	0.2812073394564294
2226	10-11	0.1192966900452106
2226	12-13	0.07000336992891931
2226	14-15	0.15587162847103997
2226	16-17	0.22905566534808486
2226	18-19	0.20102622075402365
2226	20-21	0.19045480832246398
2226	22-23	0.2873215930215096
2226	24-25	0.28353912102478773
2226	26-27	0.23793451351686912
2226	28-29	0.3411730160500994
2226	30-31	0.301768331553788
2226	32-33	0.26044594429329493
2226	34-35	0.22721743575336717
2226	36-37	0.36051964651562685
2226	38-39	0.3015212646157863
2226	40-41	0.3036993841647728
2226	42-43	0.3148584216488288
2226	44-45	0.3085695435603597
2226	46-47	0.3030855115711688
2226	48-49	0.27876143465516634
2226	50-51	0.28790024048103646
2226	52-53	0.1739450783360823
2226	54-55	0.4259037147824145
2226	56-57	0.35136587939837227
2226	58-59	0.3674703544353477
2226	60-61	0.3197683837238685
2226	62-63	0.34051142770884013
2226	64-65	0.33665963117172026
2226	66-67	0.40207913552291075
2226	68-69	0.4494248496342479
2226	70-71	0.48774628613413284
2226	72-73	0.4453719844117856
2226	74-75	0.4250589970601908
2226	76-77	0.7288611133459355
2226	78-79	0.5262093365527818
2226	80-81	0.4069498715330866
2226	82-83	0.39750598752167576
2226	84-85	0.34977045972826915
2226	86-87	0.5607076263736843
2226	88-89	0.5269870425115641
2226	90-91	0.366429022751511
2226	92-93	0.30515497369486866
2226	94-95	0.6392428988990062
2226	96-97	0.5414037992957716
2226	98-99	0.4567585640727785
2226	100	0.6411041981001802
2227	1	1.3871724569698074
2227	2	1.5641668402144173
2227	3	0.7135444410423393
2227	4	-0.0919408086525415
2227	5	3.130433053572574
2227	6	0.05258543633683033
2227	7	0.06353633511736945
2227	8	0.23478711308679578
2227	9	0.19950252217174125
2227	10-11	0.2277601085251888
2227	12-13	0.1484342334712423
2227	14-15	0.27006071631745243
2227	16-17	0.24731681248179171
2227	18-19	0.1400241709274681
2227	20-21	0.23746813796380195
2227	22-23	0.36968404696771273
2227	24-25	0.15645977773844777
2227	26-27	0.23909431922561453
2227	28-29	0.2542734460137126
2227	30-31	0.30468933439200896
2227	32-33	0.15669376178566097
2227	34-35	0.24588999038335402
2227	36-37	0.12636349818971127
2227	38-39	0.12401926478500513
2227	40-41	0.15380254851240238
2227	42-43	0.38420870739388135
2227	44-45	0.34754451490893956
2227	46-47	0.22161096326372132
2227	48-49	0.24070100130722238
2227	50-51	0.2982514799915421
2227	52-53	0.3196507301655416
2227	54-55	0.28750504082405115
2227	56-57	0.4325785267897473
2227	58-59	0.5512722058171562
2227	60-61	0.3906331567046166
2227	62-63	0.2863747585039178
2227	64-65	0.3155016137731934
2227	66-67	0.351439478109306
2227	68-69	0.21438021879531988
2227	70-71	0.30183307494431233
2227	72-73	0.21776009003361452
2227	74-75	0.39022925188478297
2227	76-77	0.4366152649177266
2227	78-79	0.4337510061038188
2227	80-81	0.16783231609547045
2227	82-83	0.25309407237603665
2227	84-85	0.3240046591421546
2227	86-87	0.3500387214348706
2227	88-89	0.3875503794599737
2227	90-91	0.33013161330153906
2227	92-93	0.3195309688029724
2227	94-95	0.4538790643003878
2227	96-97	0.2914813888843426
2227	98-99	0.30069023139324
2227	100	0.34914217181009377
2228	1	1.2646081630099246
2228	2	1.7079989887856133
2228	3	-0.14666519965214242
2228	4	-0.050137077013461706
2228	5	3.1435016019713373
2228	6	0.29424103654398337
2228	7	0.34288733428123663
2228	8	0.4688243600409905
2228	9	0.3657007810487798
2228	10-11	0.24699983552358162
2228	12-13	0.23982004352161113
2228	14-15	0.3002291868893536
2228	16-17	0.1746091608568321
2228	18-19	0.1366695147688759
2228	20-21	0.15783592053463025
2228	22-23	0.22214109029904705
2228	24-25	0.12252581773972793
2228	26-27	0.09501943184312012
2228	28-29	0.21768208913558595
2228	30-31	0.12295116248432691
2228	32-33	0.17947678106300202
2228	34-35	0.20767873528772185
2228	36-37	0.25291461076670885
2228	38-39	0.23801565986773454
2228	40-41	0.14445088391437366
2228	42-43	0.43005943604429575
2228	44-45	0.36904035305219907
2228	46-47	0.2825838280156745
2228	48-49	0.2654728007858722
2228	50-51	0.11319539546404656
2228	52-53	0.11287689966609804
2228	54-55	0.2424201401039383
2228	56-57	0.4333093470964826
2228	58-59	0.5929622350094661
2228	60-61	0.4363013249379506
2228	62-63	0.3567695924202354
2228	64-65	0.32182063448555454
2228	66-67	0.42905398341686407
2228	68-69	0.49733092748270025
2228	70-71	0.580776712670378
2228	72-73	0.5113849413958818
2228	74-75	0.3511610333099142
2228	76-77	0.446922840162415
2228	78-79	0.5154154016147388
2228	80-81	0.2758782831304387
2228	82-83	0.1656728033641528
2228	84-85	0.29401267328407954
2228	86-87	0.37492914178674397
2228	88-89	0.3458716337165839
2228	90-91	0.3808810946968677
2228	92-93	0.3598898403423547
2228	94-95	0.5860157734101819
2228	96-97	0.35550743918538075
2228	98-99	0.5664773676828361
2228	100	0.7457976418236072
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	15.0
13	27.0
14	137.0
15	463.0
16	1671.0
17	4278.0
18	7007.0
19	9017.0
20	10487.0
21	12094.0
22	14050.0
23	15834.0
24	18378.0
25	20367.0
26	23205.0
27	26234.0
28	29686.0
29	33493.0
30	38268.0
31	44544.0
32	52059.0
33	61410.0
34	75980.0
35	98304.0
36	137611.0
37	227369.0
38	558388.0
39	1031118.0
40	1141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.909375879487122	19.45000271715959	18.863891511549255	35.77672989180403
2	19.084927067466513	52.973481643066236	10.97835216979772	16.96323911966953
3	41.22473444107755	14.548574316343698	23.457055160647723	20.769636081931026
4	18.668003847005153	15.869993163926688	22.003772572263564	43.4582304168046
5	17.59758053932505	35.09177771205049	26.28331116669638	21.027330581928087
6	17.47092710081935	27.746544257208726	30.847183400682038	23.93534524128988
7	20.513547765348356	26.49893149627738	36.21986692182784	16.76765381654643
8	20.955365729922217	27.401489049550758	34.37377455061142	17.269370669915595
9	20.846850411437593	27.80162459576085	27.84346371494554	23.50806127785602
10-11	19.62023556050904	30.06477620184633	28.570868925639587	21.744119312005044
12-13	24.321338538412267	26.4704315344732	27.152824434359008	22.055405492755526
14-15	21.761297639498007	27.214642908210536	28.704025448213315	22.32003400407814
16-17	21.071892377876196	29.630910803933975	27.95125429213342	21.345942526056408
18-19	21.672409098833167	29.20774023704917	29.641938624205967	19.4779120399117
20-21	22.902882707476785	28.806311909066512	29.742305500002935	18.548499883453765
22-23	22.111328098220074	30.30284392402361	28.38363103224707	19.202196945509247
24-25	22.336624703492667	28.03826242294727	26.519439716214816	23.10567315734525
26-27	20.249115130051887	26.4253408732545	29.898007353186024	23.42753664350759
28-29	19.922205618409116	27.82058009661813	30.569630927203807	21.68758335776895
30-31	20.285047122593184	29.776992890962767	28.132021618995566	21.805938367448483
32-33	20.726817582615613	29.984800020371104	28.76801814595506	20.520364251058222
34-35	21.467424837471867	29.204469107412535	29.717311719066767	19.61079433604883
36-37	21.92115206443538	27.89394096688324	28.59660703547511	21.588299933206276
38-39	21.639516377828595	29.176951064840434	28.81743703754487	20.3660955197861
40-41	20.963901441474263	29.59012299248412	29.830760461412797	19.61521510462882
42-43	20.79323584706458	29.64012061271636	29.114271420921067	20.452372119297994
44-45	20.67876919340211	30.029733980768892	28.83700568236352	20.45449114346548
46-47	19.772489189377655	27.94495926085822	28.944770432448397	23.33778111731572
48-49	21.064370738472206	27.939501730564693	30.038607164753284	20.95752036620982
50-51	22.04373128159725	30.446851978445803	28.0603768262991	19.44903991365785
52-53	20.486851571854718	30.187586761840013	28.1475339608882	21.178027705417072
54-55	22.606722545186038	28.136055045494114	28.003245115331488	21.253977293988367
56-57	21.69165764395189	28.573910873274066	29.29824207413194	20.4361894086421
58-59	21.39630413475585	27.782463221875926	28.84412281727689	21.977109826091336
60-61	19.703098256738443	30.273925431388584	29.339888405248075	20.683087906624895
62-63	20.604125597223092	31.013815396773186	27.342155717503218	21.039903288500504
64-65	19.879810352645748	30.110013877830045	28.74955560583045	21.260620163693755
66-67	20.4823838895886	30.01004844014127	29.286952502022416	20.22061516824771
68-69	20.95808840668564	29.902434151376912	29.17522481670901	19.96425262522844
70-71	19.948327904302808	30.63238575041085	29.098167188023353	20.321119157262984
72-73	20.16122555712039	30.575229125981586	28.32998058868581	20.93356472821222
74-75	20.086910192800772	29.115717679965996	30.31120783034002	20.48616429689321
76-77	19.611166500498506	30.296908880431396	28.68028527384448	21.41163934522562
78-79	19.656511800551197	29.384322474619363	28.28210848789584	22.6770572369336
80-81	21.11110784650748	29.333678075476982	28.190622817816507	21.36459126019903
82-83	20.31118824273741	27.835687436707556	29.891151692270927	21.96197262828411
84-85	21.082959373353415	28.82321601012287	28.148246811627985	21.945577804895724
86-87	22.430331794400686	28.731115102629246	28.61782040910667	20.2207326938634
88-89	22.4906420228509	27.940794512337252	28.13365404768014	21.43490941713171
90-91	22.784593175287498	27.991291351877567	28.313017724821606	20.911097748013326
92-93	22.412840065265893	27.782722559237804	29.549210913428674	20.255226462067625
94-95	22.435091581836026	28.158902467450304	29.36207095804923	20.043934992664443
96-97	21.55566698725043	28.300246607916918	29.463182162745554	20.6809042420871
98-99	23.523907648371193	28.694114121290355	28.39984956721192	19.382128663126533
100	22.32833914758671	30.0699865041418	27.866263684388876	19.735410663882615
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	6.5
2	13.0
3	21.0
4	21.5
5	18.0
6	24.5
7	36.5
8	53.5
9	64.0
10	72.5
11	91.0
12	106.5
13	115.5
14	155.5
15	197.5
16	240.5
17	320.5
18	464.0
19	727.5
20	1138.0
21	1709.0
22	2619.5
23	4078.5
24	6398.0
25	9548.5
26	13656.5
27	18351.5
28	23338.0
29	29305.5
30	36383.5
31	44050.0
32	53015.0
33	62884.5
34	73384.0
35	85555.5
36	97343.5
37	106258.5
38	112654.0
39	117625.0
40	121072.5
41	121790.5
42	119994.0
43	118321.0
44	117400.0
45	114104.5
46	105974.0
47	96664.0
48	90434.5
49	87646.0
50	85322.5
51	79286.0
52	69487.0
53	59128.5
54	50284.0
55	42327.0
56	34253.0
57	26436.5
58	19602.5
59	14472.5
60	11447.0
61	10033.5
62	8806.0
63	7134.5
64	5689.0
65	4934.0
66	4882.5
67	4468.5
68	3507.5
69	3128.5
70	3388.0
71	3127.5
72	2111.0
73	1210.5
74	741.5
75	485.0
76	305.0
77	206.0
78	148.5
79	110.0
80	78.0
81	56.0
82	41.5
83	31.0
84	26.0
85	24.5
86	24.0
87	22.0
88	20.5
89	16.0
90	13.0
91	14.0
92	13.5
93	11.5
94	11.5
95	12.0
96	15.5
97	18.0
98	45.5
99	98.5
100	96.5
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.331114710877193
2	0.005445353526845789
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	3.3298924444740433E-4
30-31	0.009793801307276598
32-33	0.0
34-35	0.0
36-37	0.0
38-39	8.814421176548938E-4
40-41	0.0015670082091642557
42-43	7.83504104582128E-5
44-45	0.0
46-47	0.002076285877142639
48-49	0.0
50-51	0.0
52-53	0.0011556685542586387
54-55	0.019254613370105794
56-57	0.010988645066764344
58-59	0.015846370515173534
60-61	2.3505123137463837E-4
62-63	0.011811324376575578
64-65	9.793801307276598E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	9.793801307276598E-5
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2552635.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.01395424626384
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.68785375977029	65.0279869564222
2	8.221580600841348	12.334665420469664
3	2.3144190474431174	5.208411735947386
4	0.9681122295761053	2.904877059786817
5	0.5224850371334797	1.9596834334944153
6	0.32557288705557186	1.4653505792046413
7	0.21234404144401903	1.1150136346543877
8	0.1492039024629493	0.8953899770175751
9	0.10447982266994489	0.7053700173678904
>10	0.46213617338492824	5.851107531482237
>50	0.022694416490044868	1.1507366328782116
>100	0.008746113691517787	1.1799488537447247
>500	3.7196803671487775E-4	0.20145816752985446
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	3.917520522910639E-5	0.0	0.0	0.0	0.0
42-43	3.917520522910639E-5	0.0	0.0	0.0	0.0
44-45	3.917520522910639E-5	0.0	0.0	0.0	0.0
46-47	3.917520522910639E-5	0.0	0.0	0.0	0.0
48-49	3.917520522910639E-5	0.0	0.0	0.0	0.0
50-51	3.917520522910639E-5	0.0	0.0	0.0	0.0
52-53	3.917520522910639E-5	0.0	0.0	0.0	0.0
54-55	5.8762807843659586E-5	0.0	0.0	0.0	0.0
56-57	9.793801307276598E-5	0.0	1.7628842353097876E-4	0.0	0.0
58-59	1.5670082091642556E-4	0.0	3.5257684706195753E-4	0.0	0.0
60-61	2.3505123137463837E-4	0.0	3.5257684706195753E-4	0.0	0.0
62-63	3.134016418328511E-4	0.0	3.5257684706195753E-4	0.0	0.0
64-65	3.9175205229106393E-4	0.0	3.5257684706195753E-4	0.0	0.0
66-67	4.5051486013472354E-4	0.0	3.5257684706195753E-4	0.0	0.0
68-69	6.072156810511491E-4	0.0	3.5257684706195753E-4	5.876280784365959E-5	0.0
70-71	6.659784888948087E-4	0.0	3.5257684706195753E-4	1.1752561568731919E-4	0.0
72-73	7.443288993530215E-4	0.0	3.5257684706195753E-4	1.1752561568731919E-4	0.0
74-75	8.618545150403407E-4	0.0	3.5257684706195753E-4	1.1752561568731919E-4	0.0
76-77	0.0011164933490295324	0.0	3.5257684706195753E-4	1.1752561568731919E-4	0.0
78-79	0.0012536065673314045	0.0	3.5257684706195753E-4	1.1752561568731919E-4	0.0
80-81	0.001586595811778809	0.0	3.5257684706195753E-4	1.1752561568731919E-4	0.0
82-83	0.001782471837924341	0.0	3.5257684706195753E-4	1.1752561568731919E-4	0.0
84-85	0.0019391726588407665	0.0	3.5257684706195753E-4	1.1752561568731919E-4	0.0
86-87	0.001997935466684426	0.0	3.5257684706195753E-4	1.1752561568731919E-4	0.0
88	0.001997935466684426	0.0	3.5257684706195753E-4	1.1752561568731919E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	10265	0.0	78.10069	8
CACCGGC	10000	0.0	76.36837	9
CTTCACC	11215	0.0	73.6613	6
CGTAGTG	2160	0.0	71.05914	4
CGCTGCA	45	9.667201E-8	70.59165	1
GTGTGGC	3955	0.0	70.021515	7
CCTTCAC	12520	0.0	69.882355	5
TTCACCG	12270	0.0	66.67742	7
GCGTAGT	1470	0.0	65.777275	3
CAGCGTA	1050	0.0	65.045166	1
GCCTTCA	3850	0.0	60.83672	4
ACCTTCA	4695	0.0	60.584705	4
TGTGGCA	4610	0.0	59.46178	8
GCTGCCT	125	0.0	59.296986	1
CGGGGCA	1215	0.0	58.33466	94
CAGCAGC	100	0.0	58.238113	1
AGTCCTT	2625	0.0	57.940765	2
CGGGCCC	690	0.0	57.82236	7
AGCGTAG	1325	0.0	57.748436	2
GCAGCCT	65	1.8795618E-8	57.016335	1
>>END_MODULE