FastQCFastQC Report
Tue 7 Mar 2017
1SN_32A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_32A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2552635
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG102650.078.100698
CACCGGC100000.076.368379
CTTCACC112150.073.66136
CGTAGTG21600.071.059144
CGCTGCA459.667201E-870.591651
GTGTGGC39550.070.0215157
CCTTCAC125200.069.8823555
TTCACCG122700.066.677427
GCGTAGT14700.065.7772753
CAGCGTA10500.065.0451661
GCCTTCA38500.060.836724
ACCTTCA46950.060.5847054
TGTGGCA46100.059.461788
GCTGCCT1250.059.2969861
CGGGGCA12150.058.3346694
CAGCAGC1000.058.2381131
AGTCCTT26250.057.9407652
CGGGCCC6900.057.822367
AGCGTAG13250.057.7484362
GCAGCCT651.8795618E-857.0163351
Produced by FastQC (version 0.11.5)