FastQCFastQC Report
Tue 7 Mar 2017
1SN_3A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_3A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2442489
Sequences flagged as poor quality0
Sequence length94
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA129720.5310975811968857No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG74030.303092460191223No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA56720.23222213078544057No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA52610.21539503350885103No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG50880.208312094752525No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT45610.18673574374337No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG43570.17838360786885837No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG42400.17359341229377082No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA37110.15193517759957156No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT35180.14403340199280323No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT34870.14276420487461766No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA34560.14149500775643206No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA32820.13437112715758393No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA31080.1272472465587358No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG30780.1260189912830723No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC30510.12491356153497518No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC30350.12425849205462133No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC29980.12274364388130306No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA29760.12184292334581649No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC25620.10489300054166058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCTT257.290291E-787.999863
GCAGCCA202.5503508E-587.999863
AGCAGCT401.8189894E-1187.999862
GCAGCAT356.0572347E-1087.9998553
AGCAGCG700.087.9998552
CAGCAGC2900.086.482611
CAGCTGC3950.085.772011
AGCTGCC1550.085.1611562
GCTGCCC1050.083.809393
CTGCTGC1000.083.599871
AGCTGCA950.083.368282
CAGCGTA52100.082.679331
CAGCCCT143550.081.99221
AGCCCTT121050.080.403012
AGCTGCG550.079.999872
AGCAGCA1600.079.749872
AGCTGCT1050.079.618922
GCCCTTC111450.078.761643
GCTGCCA455.0931703E-1178.222093
GCAGCAC455.0931703E-1178.222093
Produced by FastQC (version 0.11.5)